Mary E. Porter

The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas

Norrander, Jan M., DeCathelineau, Aimee M., Brown, Jennifer A., Porter, Mary E., Linck, Richard W.

Ciliary and flagellar microtubules contain a specialized set of three protofilaments, termed ribbons, that are composed of tubulin and several associated proteins. Previous studies of sea urchin...

Insights into the Structural Organization of the I1 Inner Arm Dynein from a Domain Analysis of the 1β Dynein Heavy Chain

Perrone, Catherine A., Myster, Steven H., Bower, Raqual, O'Toole, Eileen T., Porter, Mary E.

To identify domains in the dynein heavy chain (Dhc) required for the assembly of an inner arm dynein, we characterized a new motility mutant (ida2-6) obtained by insertional mutagenesis. ida2-6...

Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas

Porter, Mary E., Bower, Raqual, Knott, Julie A., Byrd, Pamela, Dentler, William

A second cytoplasmic dynein heavy chain (cDhc) has recently been identified in several organisms, and its expression pattern is consistent with a possible role in axoneme assembly. We have used a...

The Chlamydomonas IDA7 Locus Encodes a 140-kDa Dynein Intermediate Chain Required to Assemble the I1 Inner Arm Complex

Perrone, Catherine A., Yang, Pinfen, O’Toole, Eileen, Sale, Winfield S., Porter, Mary E.

To identify new loci that are involved in the assembly and targeting of dynein complexes, we have screened a collection of motility mutants that were generated by insertional mutagenesis. One such...

The Chlamydomonas PF6 Locus Encodes a Large Alanine/Proline-Rich Polypeptide That Is Required for Assembly of a Central Pair Projection and Regulates Flagellar Motility

Rupp, Gerald, O'Toole, Eileen, Porter, Mary E.

Efficient motility of the eukaryotic flagellum requires precise temporal and spatial control of its constituent dynein motors. The central pair and its associated structures have been implicated as...

A Novel Dynein Light Intermediate Chain Colocalizes with the Retrograde Motor for Intraflagellar Transport at Sites of Axoneme Assembly in Chlamydomonas and Mammalian Cells

Perrone, Catherine A., Tritschler, Douglas, Taulman, Patrick, Bower, Raqual, Yoder, Bradley K., Porter, Mary E.

The assembly of cilia and flagella depends on bidirectional intraflagellar transport (IFT). Anterograde IFT is driven by kinesin II, whereas retrograde IFT requires cytoplasmic dynein 1b (cDHC1b)....

IC138 Is a WD-Repeat Dynein Intermediate Chain Required for Light Chain Assembly and Regulation of Flagellar Bending

Hendrickson, Triscia W., Perrone, Catherine A., Griffin, Paul, Wuichet, Kristin, Mueller, Joshua, Yang, Pinfen, ...

Increased phosphorylation of dynein IC IC138 correlates with decreases in flagellar microtubule sliding and phototaxis defects. To test the hypothesis that regulation of IC138 phosphorylation...

The FLA3 KAP Subunit Is Required for Localization of Kinesin-2 to the Site of Flagellar Assembly and Processive Anterograde Intraflagellar TransportV⃞

Mueller, Joshua, Perrone, Catherine A., Bower, Raqual, Cole, Douglas G., Porter, Mary E.

Intraflagellar transport (IFT) is a bidirectional process required for assembly and maintenance of cilia and flagella. Kinesin-2 is the anterograde IFT motor, and Dhc1b/Dhc2 drives retrograde IFT. To...

The Rib43a Protein Is Associated with Forming the Specialized Protofilament Ribbons of Flagellar Microtubules in Chlamydomonas

Norrander, Jan M., DeCathelineau, Aimee M., Brown, Jennifer A., Porter, Mary E., Linck, Richard W.

Ciliary and flagellar microtubules contain a specialized set of three protofilaments, termed ribbons, that are composed of tubulin and several associated proteins. Previous studies of sea urchin...

Insights into the Structural Organization of the I1 Inner Arm Dynein from a Domain Analysis of the 1β Dynein Heavy Chain

Perrone, Catherine A., Myster, Steven H., Bower, Raqual, O'Toole, Eileen T., Porter, Mary E.

To identify domains in the dynein heavy chain (Dhc) required for the assembly of an inner arm dynein, we characterized a new motility mutant (ida2-6) obtained by insertional mutagenesis. ida2-6...

Cytoplasmic Dynein Heavy Chain 1b Is Required for Flagellar Assembly in Chlamydomonas

Porter, Mary E., Bower, Raqual, Knott, Julie A., Byrd, Pamela, Dentler, William

A second cytoplasmic dynein heavy chain (cDhc) has recently been identified in several organisms, and its expression pattern is consistent with a possible role in axoneme assembly. We have used a...

The Chlamydomonas IDA7 Locus Encodes a 140-kDa Dynein Intermediate Chain Required to Assemble the I1 Inner Arm Complex

Perrone, Catherine A., Yang, Pinfen, O’Toole, Eileen, Sale, Winfield S., Porter, Mary E.

To identify new loci that are involved in the assembly and targeting of dynein complexes, we have screened a collection of motility mutants that were generated by insertional mutagenesis. One such...

The Chlamydomonas PF6 Locus Encodes a Large Alanine/Proline-Rich Polypeptide That Is Required for Assembly of a Central Pair Projection and Regulates Flagellar Motility

Rupp, Gerald, O'Toole, Eileen, Porter, Mary E.

Efficient motility of the eukaryotic flagellum requires precise temporal and spatial control of its constituent dynein motors. The central pair and its associated structures have been implicated as...

A Novel Dynein Light Intermediate Chain Colocalizes with the Retrograde Motor for Intraflagellar Transport at Sites of Axoneme Assembly in Chlamydomonas and Mammalian Cells

Perrone, Catherine A., Tritschler, Douglas, Taulman, Patrick, Bower, Raqual, Yoder, Bradley K., Porter, Mary E.

The assembly of cilia and flagella depends on bidirectional intraflagellar transport (IFT). Anterograde IFT is driven by kinesin II, whereas retrograde IFT requires cytoplasmic dynein 1b (cDHC1b)....

IC138 Is a WD-Repeat Dynein Intermediate Chain Required for Light Chain Assembly and Regulation of Flagellar Bending

Hendrickson, Triscia W., Perrone, Catherine A., Griffin, Paul, Wuichet, Kristin, Mueller, Joshua, Yang, Pinfen, ...

Increased phosphorylation of dynein IC IC138 correlates with decreases in flagellar microtubule sliding and phototaxis defects. To test the hypothesis that regulation of IC138 phosphorylation...

The FLA3 KAP Subunit Is Required for Localization of Kinesin-2 to the Site of Flagellar Assembly and Processive Anterograde Intraflagellar TransportV⃞

Mueller, Joshua, Perrone, Catherine A., Bower, Raqual, Cole, Douglas G., Porter, Mary E.

Intraflagellar transport (IFT) is a bidirectional process required for assembly and maintenance of cilia and flagella. Kinesin-2 is the anterograde IFT motor, and Dhc1b/Dhc2 drives retrograde IFT. To...

Cytoplasmic dynein nomenclature

Pfister, K. Kevin, Fisher, Elizabeth M.C., Gibbons, Ian R., Hays, Thomas S., Holzbaur, Erika L.F., McIntosh, J. Richard, ...

A variety of names has been used in the literature for the subunits of cytoplasmic dynein complexes. Thus, there is a strong need for a more definitive consensus statement on nomenclature. This is...

Domains in the 1α Dynein Heavy Chain Required for Inner Arm Assembly and Flagellar Motility in Chlamydomonas

Myster, Steven H., Knott, Julie A., Wysocki, Katrina M., O'Toole, Eileen, Porter, Mary E.

Flagellar motility is generated by the activity of multiple dynein motors, but the specific role of each dynein heavy chain (Dhc) is largely unknown, and the mechanism by which the different Dhcs are...

A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest–specific gene product

Rupp, Gerald, Porter, Mary E.

The dynein regulatory complex (DRC) is an important intermediate in the pathway that regulates flagellar motility. To identify subunits of the DRC, we characterized a Chlamydomonas motility mutant...