Scientific Commons: Martin Tabler

Prediction and preliminary validation of oncogene regulation by miRNAs (2007)

Koscianska, Edyta, Baev, Vesselin, Skreka, Konstantinia, Oikonomaki, Katerina, Rusinov, Ventsislav, Tabler, Martin, ...

Abstract Background MicroRNAs (miRNAs) are one of the most abundant groups of regulatory genes in multicellular organisms, playing important roles in many fundamental cellular processes. More than...

Representation of Unique Sequences in Libraries of Randomized Nucleic Acids (2001)

Martin Tabler, Panayiotis Benos, Technology Hellas And

From a library of nucleic acid molecules, which are randomized in parts of their sequence, unique sequence variants can be selected for specific properties. The planning of such an in vitro selection...

Replicating potato spindle tuber viroid RNA is accompanied by short RNA fragments that are characteristic of post-transcriptional gene silencing

Papaefthimiou, Ioannis, Hamilton, Andrew, Denti, Michela, Baulcombe, David, Tsagris, Mina, Tabler, Martin

The low molecular weight fraction of tomato plants inoculated with potato spindle tuber viroid (PSTVd) contains a population of short PSTVd-specific RNAs of either polarity. The main constituents...

Induction of RNA interference in Caenorhabditis elegans by RNAs derived from plants exhibiting post-transcriptional gene silencing

Boutla, Alexandra, Kalantidis, Kriton, Tavernarakis, Nektarios, Tsagris, Mina, Tabler, Martin

The term ‘gene silencing’ refers to transcriptional and post-transcriptional control of gene expression. Related processes are found across kingdoms in plants and animals. We intended to test...

A novel RNA-binding protein from Triturus carnifex identified by RNA-ligand screening with the newt hammerhead ribozyme

Denti, Michela A., Alba, A. Emilio Martínez De, Sägesser, Rudolf, Tsagris, Mina, Tabler, Martin

The newt hammerhead ribozyme is transcribed from Satellite 2 DNA, which consists of tandemly repeated units of 330 bp. However, different transcripts are synthesized in different tissues. In all...

A Bromodomain-Containing Protein from Tomato Specifically Binds Potato Spindle Tuber Viroid RNA In Vitro and In Vivo

Martínez De Alba, Angel Emilio, Sägesser, Rudolf, Tabler, Martin, Tsagris, Mina

For the identification of RNA-binding proteins that specifically interact with potato spindle tuber viroid (PSTVd), we subjected a tomato cDNA expression library prepared from viroid-infected leaves...

Characterization of the RNA motif responsible for the specific interaction of potato spindle tuber viroid RNA (PSTVd) and the tomato protein Virp1

Gozmanova, Mariyana, Denti, Michela Alessandra, Minkov, Ivan Nikiforov, Tsagris, Mina, Tabler, Martin

Viroids are small non-coding parasitic RNAs that are able to infect their host plants systemically. This circular naked RNA makes use of host proteins to accomplish its proliferation. Here we analyze...

Developmental defects by antisense-mediated inactivation of micro-RNAs 2 and 13 in Drosophila and the identification of putative target genes

Boutla, Alexandra, Delidakis, Christos, Tabler, Martin

Micro-RNAs are a class of small non-coding regulatory RNAs that impair translation by imperfect base pairing to mRNAs. For analysis of their cellular function we injected different miRNA-specific DNA...

Linear oligomeric potato spindle tuber viroid (PSTV) RNAs are accurately processed in vitro to the monomeric circular viroid proper when incubated with a nuclear extract from healthy potato cells

Tsagris, Mina, Tabler, Martin, Mühlbach, Hans-Peter, Sänger, Heinz L.

A nuclear extract for the processing of oligomeric viroid RNA in vitro has been prepared from nuclei isolated from healthy potato cells grown in suspension culture. Linear RNA molecules containing...

Infectivity studies on different potato spindle tuber viroid (PSTV) RNAs synthesized in vitro with the SP6 transcription system

Tabler, Martin, Sänger, Heinz L.

We have constructed two sets of clones in which one to six head-to-tail connected DNA copies of the potato spindle tuber viroid (PSTV) RNA genome were inserted into the plasmid pSP62- PL downstream...

MicroInspector: a web tool for detection of miRNA binding sites in an RNA sequence

Rusinov, Ventsislav, Baev, Vesselin, Minkov, Ivan Nikiforov, Tabler, Martin

Regulation of post-transcriptional gene expression by microRNAs (miRNA) has so far been validated for only a few mRNA targets. Based on the large number of miRNA genes and the possibility that one...

Viroid RNA systemic spread may depend on the interaction of a 71-nucleotide bulged hairpin with the host protein VirP1

MANIATAKI, ELSA, TABLER, MARTIN, TSAGRIS, MINA

Viroids are noncoding circular single-stranded RNAs that are propagated systemically in plants. VirP1 is a protein from tomato, which is an excellent host for potato spindle tuber viroid (PSTVd), and...