Nancy J. Sullivan, Thomas W. Geisbert, Joan B. Geisbert, Devon J. Shedlock, Ling Xu, Laurie Lamoreaux, ...
A simplified Ebola vaccine that consists of a modified GP protein (which is well-tolerated by human cells even at high concentrations) in a replication-defective adenoviral vector protects macaques.
Nancy J. Sullivan, Thomas W. Geisbert, Joan B. Geisbert, Devon J. Shedlock, Ling Xu, Laurie Lamoreaux, ...
BackgroundEbola virus causes a hemorrhagic fever syndrome that is associated with high mortality in humans. In the absence of effective therapies for Ebola virus infection, the development of a...
Shedlock, Devon J., Xu, Ling, Lamoreaux, Laurie, Custers, Jerome H., Popernack, Paul M., ...
Ebola virus causes a hemorrhagic fever syndrome that is associated with high mortality in humans. In the absence of effective therapies for Ebola virus infection, the development of a vaccine becomes...
HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells. (2006)
Betts, Michael R., Nason, Martha C., West, Sadie M., De Rosa, Stephen C., Migueles, Stephen A., Abraham, Jonathan, ...
Establishing a CD8(+) T cell-mediated immune correlate of protection in HIV disease is crucial to the development of vaccines designed to generate cell-mediated immunity. Historically, neither the...
Standardization of cytokine flow cytometry assays (2005)
Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, ...
Abstract Background Cytokine flow cytometry (CFC) or intracellular cytokine staining (ICS) can quantitate antigen-specific T cell responses in settings such as experimental vaccination....
Diversity and Recognition Efficiency of T Cell Responses to Cancer (2004)
Tor B. Stuge, Susan P. Holmes, Sahdev Saharan, Andrea Tuettenberg, Mario Roederer, Jeffrey S. Weber, ...
State-of-the art analysis of patients' response to melanoma vaccines yields lessons about cancer vaccines and rationale vaccine design in general.
Diversity and Recognition Efficiency of T Cell Responses to Cancer (2004)
Tor B. Stuge, Susan P. Holmes, Sahdev Saharan, Andrea Tuettenberg, Mario Roederer, Jeffrey S. Weber, ...
Background Melanoma patients vaccinated with tumor-associated antigens frequently develop measurable peptide-specific CD8+ T cell responses; however, such responses often do not confer clinical...
Glutathione deficiency is associated with impaired survival in HIV disease
Herzenberg, Leonore A., De Rosa, Stephen C., Dubs, J. Gregson, Roederer, Mario, Anderson, Michael T., Ela, Stephen W., ...
Glutathione (GSH), a cysteine-containing tripeptide, is essential for the viability and function of virtually all cells. In vitro studies showing that low GSH levels both promote HIV expression and...
Naïve CD4 T cells inhibit CD28-costimulated R5 HIV replication in memory CD4 T cells
Mengozzi, Manuela, Malipatlolla, Meena, De Rosa, Stephen C., Herzenberg, Leonard A., Herzenberg, Leonore A., Roederer, Mario
Stimulation with antibodies to CD3 and CD28 coimmobilized on beads can be used to significantly expand T cells ex vivo. With CD4 T cells from HIV-infected patients, this expansion usually is...
Mascola, John R., Louder, Mark K., Winter, Christine, Prabhakara, Ranjani, De Rosa, Stephen C., Douek, Daniel C., ...
There is currently intensive research on the design of novel human immunodeficiency virus type 1 (HIV-1) vaccine immunogens that can elicit potent neutralizing antibodies. A prerequisite for...
Brenchley, Jason M., Hill, Brenna J., Ambrozak, David R., Price, David A., Guenaga, Francisco J., Casazza, Joseph P., ...
Identification of T-cell subsets that are infected in vivo is essential to understanding the pathogenesis of human immunodeficiency virus (HIV) disease; however, this goal has been beset with...
Diversity and Recognition Efficiency of T Cell Responses to Cancer
Stuge, Tor B, Holmes, Susan P, Saharan, Sahdev, Tuettenberg, Andrea, Roederer, Mario, Weber, Jeffrey S, ...
State-of-the art analysis of patients' response to melanoma vaccines yields lessons about cancer vaccines and rationale vaccine design in general
Song, Kaimei, Rabin, Ronald L., Hill, Brenna J., De Rosa, Stephen C., Perfetto, Stephen P., Zhang, Hongwei H., ...
The pathways for differentiation of human CD4+ T cells into functionally distinct subsets of memory cells in vivo are unknown. The identification of these subsets and pathways has clear implications...
Nishimura, Yoshiaki, Brown, Charles R., Mattapallil, Joseph J., Igarashi, Tatsuhiko, Buckler-White, Alicia, Lafont, Bernard A. P., ...
Unlike HIV-1 and simian immunodeficiency virus (SIV), which induce a slow, unrelenting loss of immune function spanning several years, highly pathogenic simian–human immunodeficiency viruses...
Standardization of cytokine flow cytometry assays
Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, ...
Wille-Reece, Ulrike, Flynn, Barbara J., Loré, Karin, Koup, Richard A., Kedl, Ross M., Mattapallil, Joseph J., ...
Induction and maintenance of antibody and T cell responses will be critical for developing a successful vaccine against HIV. A rational approach for generating such responses is to design vaccines or...
Kuwata, Takeo, Dehghani, Houman, Brown, Charles R., Plishka, Ronald, Buckler-White, Alicia, Igarashi, Tatsuhiko, ...
A minor fraction of simian immunodeficiency virus (SIV)-infected macaques progress rapidly to AIDS in the absence of SIV-specific immune responses. Common mutations in conserved residues of env in...
Sullivan, Nancy J, Geisbert, Thomas W, Geisbert, Joan B, Shedlock, Devon J, Xu, Ling, Lamoreaux, Laurie, ...
A simplified Ebola vaccine that consists of a modified GP protein (which is well-tolerated by human cells even at high concentrations) in a replication-defective adenoviral vector protects macaques.
Chi, Bo, Dickensheets, Harold L., Spann, Kirsten M., Alston, Marc A., Luongo, Cindy, Dumoutier, Laure, ...
The mechanism by which respiratory syncytial virus (RSV) suppresses T-cell proliferation to itself and other antigens is poorly understood. We used monocyte-derived dendritic cells (MDDC) and CD4 T...
Glutathione deficiency is associated with impaired survival in HIV disease
Herzenberg, Leonore A., De Rosa, Stephen C., Dubs, J. Gregson, Roederer, Mario, Anderson, Michael T., Ela, Stephen W., ...
Glutathione (GSH), a cysteine-containing tripeptide, is essential for the viability and function of virtually all cells. In vitro studies showing that low GSH levels both promote HIV expression and...
Naïve CD4 T cells inhibit CD28-costimulated R5 HIV replication in memory CD4 T cells
Mengozzi, Manuela, Malipatlolla, Meena, De Rosa, Stephen C., Herzenberg, Leonard A., Herzenberg, Leonore A., Roederer, Mario
Stimulation with antibodies to CD3 and CD28 coimmobilized on beads can be used to significantly expand T cells ex vivo. With CD4 T cells from HIV-infected patients, this expansion usually is...
Mascola, John R., Louder, Mark K., Winter, Christine, Prabhakara, Ranjani, De Rosa, Stephen C., Douek, Daniel C., ...
There is currently intensive research on the design of novel human immunodeficiency virus type 1 (HIV-1) vaccine immunogens that can elicit potent neutralizing antibodies. A prerequisite for...
Brenchley, Jason M., Hill, Brenna J., Ambrozak, David R., Price, David A., Guenaga, Francisco J., Casazza, Joseph P., ...
Identification of T-cell subsets that are infected in vivo is essential to understanding the pathogenesis of human immunodeficiency virus (HIV) disease; however, this goal has been beset with...
Diversity and Recognition Efficiency of T Cell Responses to Cancer
Stuge, Tor B, Holmes, Susan P, Saharan, Sahdev, Tuettenberg, Andrea, Roederer, Mario, Weber, Jeffrey S, ...
State-of-the art analysis of patients' response to melanoma vaccines yields lessons about cancer vaccines and rationale vaccine design in general
Betts, Michael R., Exley, Barbara, Price, David A., Bansal, Anju, Camacho, Zenaido Tres, Teaberry, Vanessa, ...
Worldwide HIV-1 vaccine efforts are guided by the principle that HIV-specific T cell responses may provide protection from infection or delay overt disease. However, no clear correlates of T...
Song, Kaimei, Rabin, Ronald L., Hill, Brenna J., De Rosa, Stephen C., Perfetto, Stephen P., Zhang, Hongwei H., ...
The pathways for differentiation of human CD4+ T cells into functionally distinct subsets of memory cells in vivo are unknown. The identification of these subsets and pathways has clear implications...
Nishimura, Yoshiaki, Brown, Charles R., Mattapallil, Joseph J., Igarashi, Tatsuhiko, Buckler-White, Alicia, Lafont, Bernard A. P., ...
Unlike HIV-1 and simian immunodeficiency virus (SIV), which induce a slow, unrelenting loss of immune function spanning several years, highly pathogenic simian–human immunodeficiency viruses...
Standardization of cytokine flow cytometry assays
Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, ...
Wille-Reece, Ulrike, Flynn, Barbara J., Loré, Karin, Koup, Richard A., Kedl, Ross M., Mattapallil, Joseph J., ...
Induction and maintenance of antibody and T cell responses will be critical for developing a successful vaccine against HIV. A rational approach for generating such responses is to design vaccines or...
Kuwata, Takeo, Dehghani, Houman, Brown, Charles R., Plishka, Ronald, Buckler-White, Alicia, Igarashi, Tatsuhiko, ...
A minor fraction of simian immunodeficiency virus (SIV)-infected macaques progress rapidly to AIDS in the absence of SIV-specific immune responses. Common mutations in conserved residues of env in...
Sullivan, Nancy J, Geisbert, Thomas W, Geisbert, Joan B, Shedlock, Devon J, Xu, Ling, Lamoreaux, Laurie, ...
A simplified Ebola vaccine that consists of a modified GP protein (which is well-tolerated by human cells even at high concentrations) in a replication-defective adenoviral vector protects macaques.
Chi, Bo, Dickensheets, Harold L., Spann, Kirsten M., Alston, Marc A., Luongo, Cindy, Dumoutier, Laure, ...
The mechanism by which respiratory syncytial virus (RSV) suppresses T-cell proliferation to itself and other antigens is poorly understood. We used monocyte-derived dendritic cells (MDDC) and CD4 T...
A DNA Vaccine for Ebola Virus Is Safe and Immunogenic in a Phase I Clinical Trial▿ †
Martin, Julie E., Sullivan, Nancy J., Enama, Mary E., Gordon, Ingelise J., Roederer, Mario, Koup, Richard A., ...
Ebola viruses represent a class of filoviruses that causes severe hemorrhagic fever with high mortality. Recognized first in 1976 in the Democratic Republic of Congo, outbreaks continue to occur in...
Baumgarth, Nicole, Szubin, Richard, Dolganov, Greg M., Watnik, Mitchell R., Greenspan, Deborah, Da Costa, Maria, ...
Human papilloma virus (HPV) causes focal infections of epithelial layers in skin and mucosa. HIV-infected patients on highly active antiretroviral therapy (HAART) appear to be at increased risk of...
HIV nonprogressors preferentially maintain highly functional HIV-specific CD8+ T cells
Betts, Michael R., Nason, Martha C., West, Sadie M., De Rosa, Stephen C., Migueles, Stephen A., Abraham, Jonathan, ...
Establishing a CD8+ T cell–mediated immune correlate of protection in HIV disease is crucial to the development of vaccines designed to generate cell-mediated immunity. Historically, neither the...
Estimating the Infectivity of CCR5-Tropic Simian Immunodeficiency Virus SIVmac251 in the Gut▿
Wilson, David P., Mattapallil, Joseph J., Lay, Matthew D. H., Zhang, Lei, Roederer, Mario, Davenport, Miles P.
CD4+ T-cell depletion during acute human immunodeficiency virus infection occurs predominantly in the gastrointestinal mucosa. Using experimental data on SIVmac251 viral load in blood and CD4+ T...
PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infection
Petrovas, Constantinos, Casazza, Joseph P., Brenchley, Jason M., Price, David A., Gostick, Emma, Adams, William C., ...
Here, we report on the expression of programmed death (PD)-1 on human virus-specific CD8+ T cells and the effect of manipulating signaling through PD-1 on the survival, proliferation, and cytokine...
Casazza, Joseph P., Betts, Michael R., Price, David A., Precopio, Melissa L., Ruff, Laura E., Brenchley, Jason M., ...
The role of CD4+ T cells in the control of persistent viral infections beyond the provision of cognate help remains unclear. We used polychromatic flow cytometry to evaluate the production of the...
Vaccination preserves CD4 memory T cells during acute simian immunodeficiency virus challenge
Mattapallil, Joseph J., Douek, Daniel C., Buckler-White, Alicia, Montefiori, David, Letvin, Norman L., Nabel, Gary J., ...
Acute simian immunodeficiency virus (SIV)/human immunodeficiency virus infection is accompanied by a massive destruction of CD4 memory T cells across all the tissue compartments. These early events...
Precopio, Melissa L., Betts, Michael R., Parrino, Janie, Price, David A., Gostick, Emma, Ambrozak, David R., ...
Vaccinia virus immunization provides lifelong protection against smallpox, but the mechanisms of this exquisite protection are unknown. We used polychromatic flow cytometry to characterize the...
Wille-Reece, Ulrike, Flynn, Barbara J., Loré, Karin, Koup, Richard A., Miles, Aaron P., Saul, Allan, ...
There is a remarkable heterogeneity in the functional profile (quality) of T cell responses. Importantly, the magnitude and/or quality of a response required for protection may be different depending...
Price, David A., Brenchley, Jason M., Ruff, Laura E., Betts, Michael R., Hill, Brenna J., Roederer, Mario, ...
The forces that govern clonal selection during the genesis and maintenance of specific T cell responses are complex, but amenable to decryption by interrogation of constituent clonotypes within the...
Petrovas, Constantinos, Price, David A., Mattapallil, Joseph, Ambrozak, David R., Geldmacher, Christof, Cecchinato, Valentina, ...
Programmed death-1 (PD-1) is a critical mediator of virus-specific CD8+ T-cell exhaustion. Here, we examined the expression of PD-1 on simian immunodeficiency virus (SIV)-specific CD8+ T cells and...