Maria L. Ghirardi

Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga Chlamydomonas reinhardtii1

Melis, Anastasios, Zhang, Liping, Forestier, Marc, Ghirardi, Maria L., Seibert, Michael

The work describes a novel approach for sustained photobiological production of H2 gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii. This single-organism,...

Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii

Posewitz, Matthew C., Smolinski, Sharon L., Kanakagiri, Saradadevi, Melis, Anastasios, Seibert, Michael, Ghirardi, Maria L.

DNA insertional transformants of Chlamydomonas reinhardtii were screened chemochromically for attenuated H2 production. One mutant, displaying low H2 gas photoproduction, has a nonfunctional copy of...

Photosystem Electron-Transport Capacity and Light-Harvesting Antenna Size in Maize Chloroplasts 1

Ghirardi, Maria L., Melis, Anastasios

Spectrophotometric and kinetic measurements were applied to yield photosystem (PS) stoichiometries and the functional antenna size of PSI, PSIIα, and PSIIβ in Zea mays chloroplasts in situ....

Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System

King, Paul W., Posewitz, Matthew C., Ghirardi, Maria L., Seibert, Michael

Maturation of [FeFe] hydrogenases requires the biosynthesis and insertion of the catalytic iron-sulfur cluster, the H cluster. Two radical S-adenosylmethionine (SAM) proteins proposed to function in...

Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga Chlamydomonas reinhardtii1

Melis, Anastasios, Zhang, Liping, Forestier, Marc, Ghirardi, Maria L., Seibert, Michael

The work describes a novel approach for sustained photobiological production of H2 gas via the reversible hydrogenase pathway in the green alga Chlamydomonas reinhardtii. This single-organism,...

Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii

Posewitz, Matthew C., Smolinski, Sharon L., Kanakagiri, Saradadevi, Melis, Anastasios, Seibert, Michael, Ghirardi, Maria L.

DNA insertional transformants of Chlamydomonas reinhardtii were screened chemochromically for attenuated H2 production. One mutant, displaying low H2 gas photoproduction, has a nonfunctional copy of...

Photosystem Electron-Transport Capacity and Light-Harvesting Antenna Size in Maize Chloroplasts 1

Ghirardi, Maria L., Melis, Anastasios

Spectrophotometric and kinetic measurements were applied to yield photosystem (PS) stoichiometries and the functional antenna size of PSI, PSIIα, and PSIIβ in Zea mays chloroplasts in situ....

Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System

King, Paul W., Posewitz, Matthew C., Ghirardi, Maria L., Seibert, Michael

Maturation of [FeFe] hydrogenases requires the biosynthesis and insertion of the catalytic iron-sulfur cluster, the H cluster. Two radical S-adenosylmethionine (SAM) proteins proposed to function in...

Atomic Resolution Modeling of the Ferredoxin:[FeFe] Hydrogenase Complex from Chlamydomonas reinhardtii

Chang, Christopher H., King, Paul W., Ghirardi, Maria L., Kim, Kwiseon

The [FeFe] hydrogenases HydA1 and HydA2 in the green alga Chlamydomonas reinhardtii catalyze the final reaction in a remarkable metabolic pathway allowing this photosynthetic organism to produce H2...