Malcolm Buckle

Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III

Chanfreau, Guillaume, Buckle, Malcolm, Jacquier, Alain

Ribonucleases III are double-stranded RNA (dsRNA) endonucleases required for the processing of a large number of prokaryotic and eukaryotic transcripts. Although the specificity of bacterial RNase...

Stimulation of bacteriophage T4 middle transcription by the T4 proteins MotA and AsiA occurs at two distinct steps in the transcription cycle

Adelman, Karen, Brody, Edward N., Buckle, Malcolm

The bacteriophage T4 encodes proteins that are responsible for tightly regulating mRNA synthesis throughout phage development in Escherichia coli. The three classes of T4 promoters (early, middle,...

Arginine Operator Binding by Heterologous and Chimeric ArgR Repressors from Escherichia coli and Bacillus stearothermophilus

Ghochikyan, Anahit, Miltcheva Karaivanova, Iovka, Lecocq, Michèle, Vusio, Patricia, Arnaud, Marie-Claire, Snapyan, Marina, ...

Bacillus stearothermophilus ArgR binds efficiently to the Escherichia coli carAB operator, whereas the E. coli repressor binds very poorly to the argCo operator of B. stearothermophilus. In order to...

Real-time characterization of intermediates in the pathway to open complex formation by Escherichia coli RNA polymerase at the T7A1 promoter

Sclavi, Bianca, Zaychikov, Evgeny, Rogozina, Anastasia, Walther, Ferdinand, Buckle, Malcolm, Heumann, Hermann

We have used time-resolved x-ray-generated hydroxyl radical footprinting to directly characterize, at single-nucleotide resolution, several intermediates in the pathway to open complex formation by...

E2 Proteins from High- and Low-Risk Human Papillomavirus Types Differ in Their Ability To Bind p53 and Induce Apoptotic Cell Death

Parish, Joanna L., Kowalczyk, Anna, Chen, Hsin-Tien, Roeder, Geraldine E., Sessions, Richard, Buckle, Malcolm, ...

The E2 proteins from oncogenic (high-risk) human papillomaviruses (HPVs) can induce apoptotic cell death in both HPV-transformed and non-HPV-transformed cells. Here we show that the E2 proteins from...

Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III

Chanfreau, Guillaume, Buckle, Malcolm, Jacquier, Alain

Ribonucleases III are double-stranded RNA (dsRNA) endonucleases required for the processing of a large number of prokaryotic and eukaryotic transcripts. Although the specificity of bacterial RNase...

Stimulation of bacteriophage T4 middle transcription by the T4 proteins MotA and AsiA occurs at two distinct steps in the transcription cycle

Adelman, Karen, Brody, Edward N., Buckle, Malcolm

The bacteriophage T4 encodes proteins that are responsible for tightly regulating mRNA synthesis throughout phage development in Escherichia coli. The three classes of T4 promoters (early, middle,...

Arginine Operator Binding by Heterologous and Chimeric ArgR Repressors from Escherichia coli and Bacillus stearothermophilus

Ghochikyan, Anahit, Miltcheva Karaivanova, Iovka, Lecocq, Michèle, Vusio, Patricia, Arnaud, Marie-Claire, Snapyan, Marina, ...

Bacillus stearothermophilus ArgR binds efficiently to the Escherichia coli carAB operator, whereas the E. coli repressor binds very poorly to the argCo operator of B. stearothermophilus. In order to...

Real-time characterization of intermediates in the pathway to open complex formation by Escherichia coli RNA polymerase at the T7A1 promoter

Sclavi, Bianca, Zaychikov, Evgeny, Rogozina, Anastasia, Walther, Ferdinand, Buckle, Malcolm, Heumann, Hermann

We have used time-resolved x-ray-generated hydroxyl radical footprinting to directly characterize, at single-nucleotide resolution, several intermediates in the pathway to open complex formation by...

E2 Proteins from High- and Low-Risk Human Papillomavirus Types Differ in Their Ability To Bind p53 and Induce Apoptotic Cell Death

Parish, Joanna L., Kowalczyk, Anna, Chen, Hsin-Tien, Roeder, Geraldine E., Sessions, Richard, Buckle, Malcolm, ...

The E2 proteins from oncogenic (high-risk) human papillomaviruses (HPVs) can induce apoptotic cell death in both HPV-transformed and non-HPV-transformed cells. Here we show that the E2 proteins from...

Rapid coupling of Surface Plasmon Resonance (SPR and SPRi) and ProteinChip™ based mass spectrometry for the identification of proteins in nucleoprotein interactions

Bouffartigues, Emeline, Leh, Hervé, Anger-Leroy, Marielle, Rimsky, Sylvie, Buckle, Malcolm

We compared coupling approaches of SPR to LC-MS and ProteinChip™-based mass spectrometry (SELDI™) as a means of identifying proteins captured on DNA surfaces. The approach we outline has the...

High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes

Lang, Benjamin, Blot, Nicolas, Bouffartigues, Emeline, Buckle, Malcolm, Geertz, Marcel, Gualerzi, Claudio O., ...

The global transcriptional regulator H-NS selectively silences bacterial genes associated with pathogenicity and responses to environmental insults. Although there is ample evidence that H-NS binds...