Makoto M. Taketo

Carcinogenesis in Mouse Stomach by Simultaneous Activation of the Wnt Signaling and Prostaglandin E2 Pathway (2006)

Oshima, Hiroko, Matsunaga, Akihiro, Fujimura, Takashi, Tsukamoto, Tetsuya, Taketo,Makoto M., Oshima, Masanobu

Background & Aims: Accumulating evidence indicates that prostaglandin E2 (PGE2), a downstream product of cyclooxygenase 2 (COX-2), plays a key role in gastric tumorigenesis. The Wnt pathway is also...

Homozygous Inactivation of Sox9 Causes Complete XY Sex Reversal in Mice1 (2006)

Francisco Barrionuevo, Stefan Bagheri-Fam, Jürgen Klattig, Ralf Kist, Makoto M. Taketo, Christoph Englert, ...

In the presence of the Y-chromosomal gene Sry, the bipotential mouse gonads develop as testes rather than as ovaries. The autosomal gene Sox9, a likely and possibly direct Sry target, can induce...

Multiple functional defects in peripheral autonomic organs in mice lacking muscarinic acetylcholine receptor gene for the M3 subtype

Matsui, Minoru, Motomura, Daisuke, Karasawa, Hiroshi, Fujikawa, Toru, Jiang, Jian, Komiya, Yuriko, ...

Muscarinic acetylcholine receptors consist of five distinct subtypes and have been important targets for drug development. In the periphery, muscarinic acetylcholine receptors mediate cholinergic...

Early embryonic lethality caused by targeted disruption of the mouse selenocysteine tRNA gene (Trsp)

Bösl, Michael R., Takaku, Kazuaki, Oshima, Masanobu, Nishimura, Susumu, Taketo, Makoto M.

Selenoprotein biosynthesis is mediated by tRNASec, which inserts selenocysteine at UGA codons in a complex, context-specific manner. This opal suppressor serves in the conversion of serine to...

Tertiary hypothyroidism and hyperglycemia in mice with targeted disruption of the thyrotropin-releasing hormone gene

Yamada, Masanobu, Saga, Yumiko, Shibusawa, Nobuyuki, Hirato, Jyunko, Murakami, Masami, Iwasaki, Toshiharu, ...

Thyrotropin-releasing hormone (TRH) is a brain hypothalamic hormone that regulates thyrotropin (TSH) secretion from the anterior pituitary and is ubiquitously distributed throughout the brain and...

Activation of β-catenin signaling in differentiated mammary secretory cells induces transdifferentiation into epidermis and squamous metaplasias

Miyoshi, Keiko, Shillingford, Jonathan M., Le Provost, Fabienne, Gounari, Fotini, Bronson, Roderick, Von Boehmer, Harald, ...

Mammary anlagen are formed in the embryo as a derivative of the epidermis, a process that is controlled by Lef-1 and therefore possibly by β-catenin. To investigate the role of β-catenin signaling...

Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene

Sun, Jiying, Hoshino, Hideto, Takaku, Kazuaki, Nakajima, Osamu, Muto, Akihiko, Suzuki, Hiroshi, ...

Heme oxygenase-1 (HO-1) protects cells from various insults including oxidative stress. Transcriptional activators, including the Nrf2/Maf heterodimer, have been the focus of studies on the inducible...

Targeted Disruption of the Mouse Rho-Associated Kinase 2 Gene Results in Intrauterine Growth Retardation and Fetal Death

Thumkeo, Dean, Keel, Jeongsin, Ishizaki, Toshimasa, Hirose, Masaya, Nonomura, Kimiko, Oshima, Hiroko, ...

Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. However, in vivo functions of each ROCK isoform...

Genetic interaction between Wnt/β-catenin and BMP receptor signaling during formation of the AER and the dorsal–ventral axis in the limb

Soshnikova, Natalia, Zechner, Dietmar, Huelsken, Joerg, Mishina, Yuji, Behringer, Richard R., Taketo, Makoto M., ...

By conditional gene ablation in mice, we found that β-catenin, an essential downstream effector of canonical Wnt signaling, is a key regulator of formation of the apical ectodermal ridge (AER) and...

Targeted deletion of keratins 18 and 19 leads to trophoblast fragility and early embryonic lethality

Hesse, Michael, Franz, Thomas, Tamai, Yoshitaka, Taketo, Makoto M., Magin, Thomas M.

It has been reported previously that keratin 8 (K8)-deficient mice of one strain die from a liver defect at around E12.5, while those of another strain suffer from colorectal hyperplasia. These...

Hyperplastic gastric tumors induced by activated macrophages in COX-2/mPGES-1 transgenic mice

Oshima, Hiroko, Oshima, Masanobu, Inaba, Kayo, Taketo, Makoto M

Cyclooxygenase-2 (COX-2), the rate-limiting enzyme for prostanoid biosynthesis, plays a key role in gastrointestinal carcinogenesis. Among various prostanoids, prostaglandin E2 (PGE2) appears to be...

Interactions between Sox9 and β-catenin control chondrocyte differentiation

Akiyama, Haruhiko, Lyons, Jon P., Mori-Akiyama, Yuko, Yang, Xiaohong, Zhang, Ren, Zhang, Zhaoping, ...

Chondrogenesis is a multistep process that is essential for endochondral bone formation. Previous results have indicated a role for β-catenin and Wnt signaling in this pathway. Here we show the...

M3 muscarinic acetylcholine receptor plays a critical role in parasympathetic control of salivation in mice

Nakamura, Takeshi, Matsui, Minoru, Uchida, Keiko, Futatsugi, Akira, Kusakawa, Shinji, Matsumoto, Nagisa, ...

The M1 and M3 subtypes are the major muscarinic acetylcholine receptors in the salivary gland and M3 is reported to be more abundant. However, despite initial reports of salivation abnormalities in...

Carcinogenesis in Mouse Stomach by Simultaneous Activation of the Wnt Signaling and Prostaglandin E2 Pathway

Oshima, Hiroko, Matsunaga,Akihiro, Fujimura,Takashi, Tsukamoto, Tetsuya, Taketo,Makoto M., Oshima,Masanobu

Background & Aims: Accumulating evidence indicates that prostaglandin E2 (PGE2), a downstream product of cyclooxygenase 2 (COX-2), plays a key role in gastric tumorigenesis. The Wnt pathway is also...

Multiple functional defects in peripheral autonomic organs in mice lacking muscarinic acetylcholine receptor gene for the M3 subtype

Matsui, Minoru, Motomura, Daisuke, Karasawa, Hiroshi, Fujikawa, Toru, Jiang, Jian, Komiya, Yuriko, ...

Muscarinic acetylcholine receptors consist of five distinct subtypes and have been important targets for drug development. In the periphery, muscarinic acetylcholine receptors mediate cholinergic...

Early embryonic lethality caused by targeted disruption of the mouse selenocysteine tRNA gene (Trsp)

Bösl, Michael R., Takaku, Kazuaki, Oshima, Masanobu, Nishimura, Susumu, Taketo, Makoto M.

Selenoprotein biosynthesis is mediated by tRNASec, which inserts selenocysteine at UGA codons in a complex, context-specific manner. This opal suppressor serves in the conversion of serine to...

Tertiary hypothyroidism and hyperglycemia in mice with targeted disruption of the thyrotropin-releasing hormone gene

Yamada, Masanobu, Saga, Yumiko, Shibusawa, Nobuyuki, Hirato, Jyunko, Murakami, Masami, Iwasaki, Toshiharu, ...

Thyrotropin-releasing hormone (TRH) is a brain hypothalamic hormone that regulates thyrotropin (TSH) secretion from the anterior pituitary and is ubiquitously distributed throughout the brain and...

Activation of β-catenin signaling in differentiated mammary secretory cells induces transdifferentiation into epidermis and squamous metaplasias

Miyoshi, Keiko, Shillingford, Jonathan M., Le Provost, Fabienne, Gounari, Fotini, Bronson, Roderick, Von Boehmer, Harald, ...

Mammary anlagen are formed in the embryo as a derivative of the epidermis, a process that is controlled by Lef-1 and therefore possibly by β-catenin. To investigate the role of β-catenin signaling...

Hemoprotein Bach1 regulates enhancer availability of heme oxygenase-1 gene

Sun, Jiying, Hoshino, Hideto, Takaku, Kazuaki, Nakajima, Osamu, Muto, Akihiko, Suzuki, Hiroshi, ...

Heme oxygenase-1 (HO-1) protects cells from various insults including oxidative stress. Transcriptional activators, including the Nrf2/Maf heterodimer, have been the focus of studies on the inducible...

Targeted Disruption of the Mouse Rho-Associated Kinase 2 Gene Results in Intrauterine Growth Retardation and Fetal Death

Thumkeo, Dean, Keel, Jeongsin, Ishizaki, Toshimasa, Hirose, Masaya, Nonomura, Kimiko, Oshima, Hiroko, ...

Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. However, in vivo functions of each ROCK isoform...

Genetic interaction between Wnt/β-catenin and BMP receptor signaling during formation of the AER and the dorsal–ventral axis in the limb

Soshnikova, Natalia, Zechner, Dietmar, Huelsken, Joerg, Mishina, Yuji, Behringer, Richard R., Taketo, Makoto M., ...

By conditional gene ablation in mice, we found that β-catenin, an essential downstream effector of canonical Wnt signaling, is a key regulator of formation of the apical ectodermal ridge (AER) and...

Targeted deletion of keratins 18 and 19 leads to trophoblast fragility and early embryonic lethality

Hesse, Michael, Franz, Thomas, Tamai, Yoshitaka, Taketo, Makoto M., Magin, Thomas M.

It has been reported previously that keratin 8 (K8)-deficient mice of one strain die from a liver defect at around E12.5, while those of another strain suffer from colorectal hyperplasia. These...

Hyperplastic gastric tumors induced by activated macrophages in COX-2/mPGES-1 transgenic mice

Oshima, Hiroko, Oshima, Masanobu, Inaba, Kayo, Taketo, Makoto M

Cyclooxygenase-2 (COX-2), the rate-limiting enzyme for prostanoid biosynthesis, plays a key role in gastrointestinal carcinogenesis. Among various prostanoids, prostaglandin E2 (PGE2) appears to be...

Interactions between Sox9 and β-catenin control chondrocyte differentiation

Akiyama, Haruhiko, Lyons, Jon P., Mori-Akiyama, Yuko, Yang, Xiaohong, Zhang, Ren, Zhang, Zhaoping, ...

Chondrogenesis is a multistep process that is essential for endochondral bone formation. Previous results have indicated a role for β-catenin and Wnt signaling in this pathway. Here we show the...

M3 muscarinic acetylcholine receptor plays a critical role in parasympathetic control of salivation in mice

Nakamura, Takeshi, Matsui, Minoru, Uchida, Keiko, Futatsugi, Akira, Kusakawa, Shinji, Matsumoto, Nagisa, ...

The M1 and M3 subtypes are the major muscarinic acetylcholine receptors in the salivary gland and M3 is reported to be more abundant. However, despite initial reports of salivation abnormalities in...

Carcinogenesis in Mouse Stomach by Simultaneous Activation of the Wnt Signaling and Prostaglandin E2 Pathway

Oshima, Hiroko, Matsunaga, Akihiro, Fujimura, Takashi, Tsukamoto, Tetsuya, Taketo,Makoto M., Oshima, Masanobu

Background & Aims: Accumulating evidence indicates that prostaglandin E2 (PGE2), a downstream product of cyclooxygenase 2 (COX-2), plays a key role in gastric tumorigenesis. The Wnt pathway is also...

Continuous tooth generation in mouse is induced by activated epithelial Wnt/β-catenin signaling

Järvinen, Elina, Salazar-Ciudad, Isaac, Birchmeier, Walter, Taketo, Makoto M., Jernvall, Jukka, Thesleff, Irma

The single replacement from milk teeth to permanent teeth makes mammalian teeth different from teeth of most nonmammalian vertebrates and other epithelial organs such as hair and feathers, whose...

Wnt/β-catenin signaling promotes expansion of Isl-1–positive cardiac progenitor cells through regulation of FGF signaling

Cohen, Ethan David, Wang, Zhishan, Lepore, John J., Lu, Min Min, Taketo, Makoto M., Epstein, Douglas J., ...

The anterior heart field (AHF), which contributes to the outflow tract and right ventricle of the heart, is defined in part by expression of the LIM homeobox transcription factor Isl-1. The...

Wnt signaling regulates pancreatic β cell proliferation

Rulifson, Ingrid C., Karnik, Satyajit K., Heiser, Patrick W., Ten Berge, Derk, Chen, Hainan, Gu, Xueying, ...

There is widespread interest in defining factors and mechanisms that stimulate proliferation of pancreatic islet cells. Wnt signaling is an important regulator of organ growth and cell fates, and...

Canonical Wnt signaling is a positive regulator of mammalian cardiac progenitors

Kwon, Chulan, Arnold, Joshua, Hsiao, Edward C., Taketo, Makoto M., Conklin, Bruce R., Srivastava, Deepak

Guiding multipotent cells into distinct lineages and controlling their expansion remain fundamental challenges in developmental and stem cell biology. Members of the Wnt pathway control many pivotal...

ROCK-I regulates closure of the eyelids and ventral body wall by inducing assembly of actomyosin bundles

Shimizu, Yoshihiko, Thumkeo, Dean, Keel, Jeongsin, Ishizaki, Toshimasa, Oshima, Hiroko, Oshima, Masanobu, ...

Rho-associated kinase (ROCK) I mediates signaling from Rho to the actin cytoskeleton. To investigate the in vivo functions of ROCK-I, we generated ROCK-I–deficient mice. Loss of ROCK-I resulted in...

Cytokeratins 8 and 19 in the Mouse Placental Development

Tamai, Yoshitaka, Ishikawa, Tomo-o, Bösl, Michael R., Mori, Masahiko, Nozaki, Masami, Baribault, Heléne, ...

To investigate the expression and biological roles of cytokeratin 19 (K19) in development and in adult tissues, we inactivated the mouse K19 gene (Krt1-19) by inserting a bacterial β-galactosidase...

Distinct roles of Wnt/β-catenin and Bmp signaling during early cardiogenesis

Klaus, Alexandra, Saga, Yumiko, Taketo, Makoto M., Tzahor, Eldad, Birchmeier, Walter

Heart formation requires the coordinated recruitment of multiple cardiac progenitor cell populations derived from both the first and second heart fields. In this study, we have ablated the Bmp...