The Genomic Pattern of tDNA Operon Expression in E. coli (2005)
David H Ardell, Leif A Kirsebom
In fast-growing microorganisms, a tRNA concentration profile enriched in major isoacceptors selects for the biased usage of cognate codons. This optimizes translational rate for the least mass...
Thesis (doctoral)--Uppsala University, 1985.
Inhibition of RNase P RNA cleavage by aminoglycosides
Mikkelsen, Nils E., Brännvall, Mathias, Virtanen, Anders, Kirsebom, Leif A.
A number of aminoglycosides have been reported to interact and interfere with the function of various RNA molecules. Among these are 16S rRNA, the group I intron, and the hammerhead ribozymes. In...
Brännvall, Mathias, Mikkelsen, Nils E., Kirsebom, Leif A.
Lead(II)-induced cleavage can be used as a tool to probe conformational changes in RNA. In this report, we have investigated the conformation of M1 RNA, the catalytic subunit of Escherichia coli...
Metal ion cooperativity in ribozyme cleavage of RNA
Brännvall, Mathias, Kirsebom, Leif A.
Combinations of chemical and genetic approaches were used to study the function of divalent metal ions in cleavage of RNA by the ribozyme RNase P RNA. We show that different divalent metal ions have...
Cross talk between the +73/294 interaction and the cleavage site in RNase P RNA mediated cleavage
Brännvall, Mathias, Kikovska, Ema, Kirsebom, Leif A.
To monitor functionally important metal ions and possible cross talk in RNase P RNA mediated cleavage we studied cleavage of substrates, where the 2′OH at the RNase P cleavage site (at −1) and/or...
Kikovska, Ema, Brännvall, Mathias, Kufel, Joanna, Kirsebom, Leif A.
Like the translational elongation factor EF-Tu, RNase P interacts with a large number of substrates where RNase P with its RNA subunit generates tRNAs with matured 5′ termini by cleaving tRNA...
The Genomic Pattern of tDNA Operon Expression in E. coli
Ardell, David H, Kirsebom, Leif A
In fast-growing microorganisms, a tRNA concentration profile enriched in major isoacceptors selects for the biased usage of cognate codons. This optimizes translational rate for the least mass...
The naturally trans-acting ribozyme RNase P RNA has leadzyme properties
Kikovska, Ema, Mikkelsen, Nils-Egil, Kirsebom, Leif A.
Divalent metal ions promote hydrolysis of RNA backbones generating 5′OH and 2′;3′P as cleavage products. In these reactions, the neighboring 2′OH act as the nucleophile. RNA catalyzed...
Inhibition of Klenow DNA polymerase and poly(A)-specific ribonuclease by aminoglycosides.
Ren, Yan-Guo, Martínez, Javier, Kirsebom, Leif A, Virtanen, Anders
Aminoglycosides are known to bind and perturb the function of catalytic RNA. Here we show that they also are potent inhibitors of protein-based catalysis using Escherichia coli Klenow polymerase...
Lead(II) cleavage analysis of RNase P RNA in vivo
LINDELL, MAGNUS, BRÄNNVALL, MATHIAS, WAGNER, E. GERHART H., KIRSEBOM, LEIF A.
The overall conformation of M1 RNA, the catalytic RNA subunit of RNase P in Escherichia coli, was analyzed in vivo and, in the presence of the C5 protein subunit, in vitro by lead(II) acetate...
Hinas, Andrea, Larsson, Pontus, Avesson, Lotta, Kirsebom, Leif A., Virtanen, Anders, Söderbom, Fredrik
Most eukaryotic mRNAs depend upon precise removal of introns by the spliceosome, a complex of RNAs and proteins. Splicing of pre-mRNA is known to take place in Dictyostelium discoideum, and we...
Inhibition of RNase P RNA cleavage by aminoglycosides
Mikkelsen, Nils E., Brännvall, Mathias, Virtanen, Anders, Kirsebom, Leif A.
A number of aminoglycosides have been reported to interact and interfere with the function of various RNA molecules. Among these are 16S rRNA, the group I intron, and the hammerhead ribozymes. In...
Brännvall, Mathias, Mikkelsen, Nils E., Kirsebom, Leif A.
Lead(II)-induced cleavage can be used as a tool to probe conformational changes in RNA. In this report, we have investigated the conformation of M1 RNA, the catalytic subunit of Escherichia coli...
Metal ion cooperativity in ribozyme cleavage of RNA
Brännvall, Mathias, Kirsebom, Leif A.
Combinations of chemical and genetic approaches were used to study the function of divalent metal ions in cleavage of RNA by the ribozyme RNase P RNA. We show that different divalent metal ions have...
Cross talk between the +73/294 interaction and the cleavage site in RNase P RNA mediated cleavage
Brännvall, Mathias, Kikovska, Ema, Kirsebom, Leif A.
To monitor functionally important metal ions and possible cross talk in RNase P RNA mediated cleavage we studied cleavage of substrates, where the 2′OH at the RNase P cleavage site (at −1) and/or...
Kikovska, Ema, Brännvall, Mathias, Kufel, Joanna, Kirsebom, Leif A.
Like the translational elongation factor EF-Tu, RNase P interacts with a large number of substrates where RNase P with its RNA subunit generates tRNAs with matured 5′ termini by cleaving tRNA...
The Genomic Pattern of tDNA Operon Expression in E. coli
Ardell, David H, Kirsebom, Leif A
In fast-growing microorganisms, a tRNA concentration profile enriched in major isoacceptors selects for the biased usage of cognate codons. This optimizes translational rate for the least mass...
The naturally trans-acting ribozyme RNase P RNA has leadzyme properties
Kikovska, Ema, Mikkelsen, Nils-Egil, Kirsebom, Leif A.
Divalent metal ions promote hydrolysis of RNA backbones generating 5′OH and 2′;3′P as cleavage products. In these reactions, the neighboring 2′OH act as the nucleophile. RNA catalyzed...
Inhibition of Klenow DNA polymerase and poly(A)-specific ribonuclease by aminoglycosides.
Ren, Yan-Guo, Martínez, Javier, Kirsebom, Leif A, Virtanen, Anders
Aminoglycosides are known to bind and perturb the function of catalytic RNA. Here we show that they also are potent inhibitors of protein-based catalysis using Escherichia coli Klenow polymerase...
Lead(II) cleavage analysis of RNase P RNA in vivo
LINDELL, MAGNUS, BRÄNNVALL, MATHIAS, WAGNER, E. GERHART H., KIRSEBOM, LEIF A.
The overall conformation of M1 RNA, the catalytic RNA subunit of RNase P in Escherichia coli, was analyzed in vivo and, in the presence of the C5 protein subunit, in vitro by lead(II) acetate...
Hinas, Andrea, Larsson, Pontus, Avesson, Lotta, Kirsebom, Leif A., Virtanen, Anders, Söderbom, Fredrik
Most eukaryotic mRNAs depend upon precise removal of introns by the spliceosome, a complex of RNAs and proteins. Splicing of pre-mRNA is known to take place in Dictyostelium discoideum, and we...
Eukaryotic RNase P RNA mediates cleavage in the absence of protein
Kikovska, Ema, Svärd, Staffan G., Kirsebom, Leif A.
The universally conserved ribonucleoprotein RNase P is involved in the processing of tRNA precursor transcripts. RNase P consists of one RNA and, depending on its origin, a variable number of protein...
Larsson, Pontus, Hinas, Andrea, Ardell, David H., Kirsebom, Leif A., Virtanen, Anders, Söderbom, Fredrik
Genome data are increasingly important in the computational identification of novel regulatory non-coding RNAs (ncRNAs). However, most ncRNA gene-finders are either specialized to well-characterized...