Lawrence M. Lifshitz

Insulin stimulates membrane fusion and GLUT4 accumulation in clathrin coats on adipocyte plasma membranes (2007)

Huang, Shaohui, Lifshitz, Lawrence M., Jones, Christine, Bellve, Karl D., Standley, Clive, Fonseca, Sonya G., ...

Total internal reflection fluorescence (TIRF) microscopy reveals highly mobile structures containing enhanced green fluorescent protein-tagged glucose transporter 4 (GLUT4) within a zone about 100 nm...

Supervillin modulation of focal adhesions involving TRIP6/ZRP-1 (2006)

Takizawa, Norio, Smith, Tara C., Nebl, Thomas, Crowley, Jessica L., Palmieri, Stephen J., Lifshitz, Lawrence M., ...

Cell-substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that supervillin (SV)--a peripheral membrane protein that binds myosin II and F-actin in such...

Dihydropyridine receptors and type 1 ryanodine receptors constitute the molecular machinery for voltage-induced Ca2+ release in nerve terminals (2006)

De Crescenzo, Valerie, Fogarty, Kevin E., ZhuGe, Ronghua, Tuft, Richard A., Lifshitz, Lawrence M., Carmichael, Jeffrey, ...

Ca2+ stores were studied in a preparation of freshly dissociated terminals from hypothalamic magnocellular neurons. Depolarization from a holding level of -80 mV in the absence of extracellular Ca2+...

Plasma membrane domains specialized for clathrin-mediated endocytosis in primary cells (2006)

Bellve, Karl D., Leonard, Deborah, Standley, Clive, Lifshitz, Lawrence M., Tuft, Richard A., Hayakawa, Akira, ...

Clathrin assembly at the plasma membrane is a fundamental process required for endocytosis. In cultured cells, most of the clathrin is localized to large patches that display little lateral mobility....

Syntillas release Ca2+ at a site different from the microdomain where exocytosis occurs in mouse chromaffin cells (2006)

Zhuge, Ronghua, DeCrescenzo, Valerie, Sorrentino, Vincenzo, Lai, F. Anthony, Tuft, Richard A., Lifshitz, Lawrence M., ...

Spontaneous, short-lived, focal cytosolic Ca2+ transients were found for the first time and characterized in freshly dissociated chromaffin cells from mouse. Produced by release of Ca2+ from...

Syntillas release Ca2+ at a site different from the microdomain where exocytosis occurs in mouse chromaffin cells. (2006)

ZhuGe, Ronghua, DeCrescenzo, Valerie, Sorrentino, Vincenzo, Lai, F. Anthony, Tuft, Richard A., Lifshitz, Lawrence M., ...

Spontaneous, short-lived, focal cytosolic Ca2+ transients were found for the first time and characterized in freshly dissociated chromaffin cells from mouse. Produced by release of Ca2+ from...

Using total fluorescence increase (signal mass) to determine the Ca2+ current underlying localized Ca2+ events (2004)

Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.

The feasibility of determining localized Ca(2+) influx using only wide-field fluorescence images was explored by imaging (using fluo-3) single channel Ca(2+) fluorescence transients (SCCaFTs), due to...

Ca(2+) spark sites in smooth muscle cells are numerous and differ in number of ryanodine receptors, large-conductance K(+) channels, and coupling ratio between them (2004)

Zhuge, Ronghua, Fogarty, Kevin E., Baker, Stephen P., McCarron, John G., Tuft, Richard A., Lifshitz, Lawrence M., ...

Ca(2+) sparks are highly localized Ca(2+) transients caused by Ca(2+) release from sarcoplasmic reticulum through ryanodine receptors (RyR). In smooth muscle, Ca(2+) sparks activate nearby...

Essential role of Ca2+/calmodulin in Early Endosome Antigen-1 localization (2003)

Lawe, Deirdre C., Sitouah, Nachida, Hayes, Susan, Chawla, Anil, Virbasius, Joseph V., Tuft, Richard A., ...

Ca2+ is an essential requirement in membrane fusion, acting through binding proteins such as calmodulin (CaM). Ca2+/CaM is required for early endosome fusion in vitro, however, the molecular basis...

Visualization of single molecules of mRNA in situ (2003)

Femino, Andrea M., Fogarty, Kevin E., Lifshitz, Lawrence M., Carrington, Walter A., Singer, Robert H.

Rho-dependent agonist-induced spatio-temporal change in myosin phosphorylation in smooth muscle cells (2001)

Miyazaki, Koji, Yano, Takeo, Schmidt, David J., Tokui, Toshiya, Shibata, Masao, Lifshitz, Lawrence M., ...

Agonist-induced translocation of RhoA and the spatio-temporal change in myosin regulatory light chain (MLC20) phosphorylation in smooth muscle was clarified at the single cell level. We expressed...

Sequential roles for phosphatidylinositol 3-phosphate and Rab5 in tethering and fusion of early endosomes via their interaction with EEA1 (2001)

Lawe, Deirdre C., Chawla, Anil, Merithew, Eric Lee, Dumas, John J., Carrington, Walter A., Fogarty, Kevin E., ...

Early endosome antigen 1 (EEA1) is a 170-kDa polypeptide required for endosome fusion in mammalian cells. The COOH terminus of EEA1 contains a FYVE domain that interacts specifically with...

Dynamics of signaling between Ca(2+) sparks and Ca(2+)- activated K(+) channels studied with a novel image-based method for direct intracellular measurement of ryanodine receptor Ca(2+) current (2000)

ZhuGe, Ronghua, Fogarty, Kevin E., Tuft, Richard A., Lifshitz, Lawrence M., Sayar, Kemal, Walsh, John V.

Ca(2+) sparks are highly localized cytosolic Ca(2+) transients caused by a release of Ca(2+) from the sarcoplasmic reticulum via ryanodine receptors (RyRs); they are the elementary events underlying...

Imaging Ca(2+) entering the cytoplasm through a single opening of a plasma membrane cation channel (1999)

Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.

Discrete localized fluorescence transients due to openings of a single plasma membrane Ca(2+) permeable cation channel were recorded using wide-field digital imaging microscopy with fluo-3 as the...

Sorting of beta-actin mRNA and protein to neurites and growth cones in culture (1998)

Bassell, Gary J., Zhang, Honglai, Byrd, Anne L., Femino, Andrea M., Singer, Robert H., Taneja, Krishan L., ...

The transport of mRNAs into developing dendrites and axons may be a basic mechanism to localize cytoskeletal proteins to growth cones and influence microfilament organization. Using isoform-specific...

Poly(A) RNA codistribution with microfilaments: evaluation by in situ hybridization and quantitative digital imaging microscopy (1992)

Taneja, Krishan L., Lifshitz, Lawrence M., Fay, Fredric S., Singer, Robert H.

The distribution of poly(A) RNA has been visualized in single cells using high-resolution fluorescent in situ hybridization. Digital imaging microscopy was used to quantitate the signal in various...

Visualization of Ca2+ entry through single stretch-activated cation channels

Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.

Stretch-activated channels (SACs) have been found in smooth muscle and are thought to be involved in myogenic responses. Although SACs have been shown to be Ca2+ permeable when Ca2+ is the only...

Visualization of Ca2+ entry through single stretch-activated cation channels

Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.

Stretch-activated channels (SACs) have been found in smooth muscle and are thought to be involved in myogenic responses. Although SACs have been shown to be Ca2+ permeable when Ca2+ is the only...

Syntillas Release Ca2+ at a Site Different from the Microdomain Where Exocytosis Occurs in Mouse Chromaffin Cells

ZhuGe, Ronghua, DeCrescenzo, Valerie, Sorrentino, Vincenzo, Lai, F. Anthony, Tuft, Richard A., Lifshitz, Lawrence M., ...

Spontaneous, short-lived, focal cytosolic Ca2+ transients were found for the first time and characterized in freshly dissociated chromaffin cells from mouse. Produced by release of Ca2+ from...

Porphyrin Loading of Lipofuscin Granules in Inflamed Striated Muscle

Kiefer, Charles R., McKenney, James B., Trainor, Jane F., Lambrecht, Richard W., Bonkovsky, Herbert L., Lifshitz, Lawrence M., ...

To further the understanding of oxidative effects on inflammation injury to muscle fiber structure, fluorescent imaging analysis of human striated muscle tissues from a variety of inflammatory or...

Supervillin modulation of focal adhesions involving TRIP6/ZRP-1

Takizawa, Norio, Smith, Tara C., Nebl, Thomas, Crowley, Jessica L., Palmieri, Stephen J., Lifshitz, Lawrence M., ...

Cell–substrate contacts, called focal adhesions (FAs), are dynamic in rapidly moving cells. We show that supervillin (SV)—a peripheral membrane protein that binds myosin II and F-actin in such...

Using Total Fluorescence Increase (Signal Mass) to Determine the Ca2+ Current Underlying Localized Ca2+ Events

Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.

The feasibility of determining localized Ca2+ influx using only wide-field fluorescence images was explored by imaging (using fluo-3) single channel Ca2+ fluorescence transients (SCCaFTs), due to...

Correction

Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.

Relationship of Ca2+ sparks to STOCs studied with 2D and 3D imaging in feline oesophageal smooth muscle cells

Kirber, Michael T, Etter, Elaine F, Bellvé, Karl A, Lifshitz, Lawrence M, Tuft, Richard A, Fay, Fredric S, ...

We recorded Ca2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording and a unique, high-speed widefield digital imaging...

Imaging Ca2+ Entering the Cytoplasm through a Single Opening of a Plasma Membrane Cation Channel

Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.

Discrete localized fluorescence transients due to openings of a single plasma membrane Ca2+ permeable cation channel were recorded using wide-field digital imaging microscopy with fluo-3 as the Ca2+...

Dynamics of Signaling between Ca2+ Sparks and Ca2+- Activated K+ Channels Studied with a Novel Image-Based Method for Direct Intracellular Measurement of Ryanodine Receptor Ca2+ Current

ZhuGe, Ronghua, Fogarty, Kevin E., Tuft, Richard A., Lifshitz, Lawrence M., Sayar, Kemal, Walsh, John V.

Ca2+ sparks are highly localized cytosolic Ca2+ transients caused by a release of Ca2+ from the sarcoplasmic reticulum via ryanodine receptors (RyRs); they are the elementary events underlying global...