Homologous high-throughput expression and purification of highly conserved E coliproteins (2007)
Ergin, Asgar, Büssow, Konrad, Sieper, Joachim, Thiel, Andreas, Duchmann, Rainer, Adam, Thomas
Abstract Background Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that...
Homologous high-throughput expression and purification of highly conserved E coli proteins (2007)
Ergin, Asgar, Büssow, Konrad, Sieper, Joachim, Thiel, Andreas, Duchmann, Rainer, Adam, Thomas
Background Genetic factors and a dysregulated immune response towards commensal bacteria contribute to the pathogenesis of Inflammatory Bowel Disease (IBD). Animal models demonstrated that the normal...
Vectors for co-expression of an unrestricted number of proteins (2007)
Scheich, Christoph, Kümmel, Daniel, Soumailakakis, Dimitri, Heinemann, Udo, Büssow, Konrad
A vector system is presented that allows generation of E. coli co-expression clones by a standardized, robust cloning procedure. The number of co-expressed proteins is not limited. Five ‘pQLink’...
Manjasetty, Babu A., Büssow, Konrad, Fieber-Erdmann, Martin, Roske, Yvette, Gobom, Johan, Scheich, Christoph, ...
The human protein PTD012 is the longer product of an alternatively spliced gene and was described to be localized in the nucleus. The X-ray structure analysis at 1.7 Å resolution of PTD012 through...
Recent advances of protein microarrays (2006)
Hultschig, Claus, Kreutzberger, Jürgen, Seitz, Harald, Konthur, Zoltán, Büssow, Konrad, Lehrach, Hans
Technological innovations and novel applications have greatly advanced the field of protein microarrays. Over the past two years, different types of protein microarrays have been used for serum...
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1) (2005)
Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...
Abstract Background Human Aortic Preferentially Expressed Protein-1 (APEG-1) is a novel specific smooth muscle differentiation marker thought to play a role in the growth and differentiation of...
Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...
Abstract Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural...
Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...
Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics...
Cepok, Sabine, Zhou, Dun, Srivastava, Rajneesh, Nessler, Stefan, Stei, Susanne, Büssow, Konrad, ...
MS is a chronic inflammatory and demyelinating disease of the CNS with as yet unknown etiology. A hallmark of this disease is the occurrence of oligoclonal IgG antibodies in the cerebrospinal fluid...
The structure of the TRAPP subunit TPC6 suggests a model for a TRAPP subcomplex (2005)
Kümmel, Daniel, Müller, Jürgen J., Roske, Yvette, Misselwitz, Rolf, Büssow, Konrad, Heinemann, Udo
The TRAPP (transport protein particle) complexes are tethering complexes that have an important role at the different steps of vesicle transport. Recently, the crystal structures of the TRAPP...
The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L (2004)
Soukenik,Michael, Diehl,Anne, Leidert,Martina, Sievert,Volker, Büssow,Konrad, Leitner,Dietmar, ...
The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171–270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains'...
A catalog of human cDNA expression clones and its application to structural genomics (2004)
Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...
Abstract We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli . A cDNA expression library...
A catalog of human cDNA expression clones and its application to structural genomics (2004)
Büssow,Konrad, Quedenau,Claudia, Sievert,Volker, Tischer,Janett, Scheich,Christoph, Seitz,Harald, ...
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825...
Protein arrays from cDNA expression libraries (2004)
Weiner,Hendrik, Faupel,Thomas, Büssow,Konrad
This chapter describes the production of a cDNA expression library from human fetal brain, the construction of a high-density protein array from such a library, and two applications to screen the...
Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...
Abstract Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...
Scheich,Christoph, Leitner,Dietmar, Sievert,Volker, Leidert,Martina, Schlegel,Brigitte, Simon,Bernd, ...
Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...
An automated in vitro protein folding screen applied to a human dynactin subunit (2004)
Scheich,Christoph, Niesen,Frank H., Seckler,Robert, Büssow,Konrad
The preparation of proteins for structural and functional analysis using the Escherichia coli expression system is often hampered by the formation of insoluble intracellular protein aggregates...
A catalog of human cDNA expression clones and its application to structural genomics (2004)
Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825...
Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...
Background High-throughput protein structure analysis of individual protein domains requires analysis of large numbers of expression clones to identify suitable constructs for structure...
An automated in vitro protein folding screen applied to a human dynactin subunit (2004)
Scheich, Christoph, Niesen, Frank H., Seckler, Robert, Büssow, Konrad
The preparation of proteins for structural and functional analysis using the Escherichia coli expression system is often hampered by the formation of insoluble intracellular protein aggregates...
The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L (2004)
Soukenik, Michael, Diehl, Anne, Leidert, Martina, Sievert, Volker, Büssow, Konrad, Leitner, Dietmar, ...
The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171–270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains'...
Protein arrays from cDNA expression libraries (2004)
Weiner, Hendrik, Faupel, Thomas, Büssow, Konrad
This chapter describes the production of a cDNA expression library from human fetal brain, the construction of a high-density protein array from such a library, and two applications to screen the...
Mueller,Uwe, Büssow,Konrad, Diehl,Anne, Bartl,Franz J., Niesen,Frank H., Nyarsik,Lajos, ...
Small peptide tags are often fused to proteins to allow their affinity purification in high-throughput structure analysis schemes. To assess the compatibility of small peptide tags with protein...
Scheich, Christoph, Sievert, Volker, Büssow, Konrad
Abstract Background Functional Genomics, the systematic characterisation of the functions of an organism's genes, includes the study of the gene products, the proteins. Such studies require methods...
Mueller, Uwe, Büssow, Konrad, Diehl, Anne, Bartl, Franz J., Niesen, Frank H., Nyarsik, Lajos, ...
Small peptide tags are often fused to proteins to allow their affinity purification in high-throughput structure analysis schemes. To assess the compatibility of small peptide tags with protein...
Büssow, Konrad, Hoffmann, Steve, Sievert, Volker
Abstract Background Functional genomics involves the parallel experimentation with large sets of proteins. This requires management of large sets of open reading frames as a prerequisite of the...
High-throughput protein arrays: prospects for molecular diagnostics (2002)
Walter,Gerald, Büssow,Konrad, Lueking,Angelika, Glökler,Jörn
High-throughput protein arrays allow the miniaturized and parallel analysis of large numbers of diagnostic markers in complex samples. Using automated colony picking and gridding, cDNA or antibody...
Genetic analysis of the mouse brain proteome (2002)
Klose,Joachim, Nock,Christina, Herrmann,Marion, Stühler,Kai, Marcus,Katrin, Blüggel,Martin, ...
Proteome analysis is a fundamental step in systematic functional genomics. Here we have resolved 8,767 proteins from the mouse brain proteome by large-gel two-dimensional electrophoresis. We detected...
Protein Array Technology: The Tool to Bridge Genomics and Proteomics (2002)
Eickhoff,Holger, Konthur,Zoltán, Lueking,Angelika, Lehrach,Hans, Walter,Gerald, Nordhoff,Eckhard, ...
The generation of protein chips requires much more efforts than DNA microchips. While DNA is DNA and a variety of different DNA molecules behave stable in a hybridisation experiment, proteins are...
High-throughput protein arrays: prospects for molecular diagnostics (2002)
Walter, Gerald, Büssow, Konrad, Lueking, Angelika, Glökler, Jörn
High-throughput protein arrays allow the miniaturized and parallel analysis of large numbers of diagnostic markers in complex samples. Using automated colony picking and gridding, cDNA or antibody...
Genetic analysis of the mouse brain proteome (2002)
Klose, Joachim, Nock, Christina, Herrmann, Marion, Stühler, Kai, Marcus, Katrin, Blüggel, Martin, ...
Proteome analysis is a fundamental step in systematic functional genomics. Here we have resolved 8,767 proteins from the mouse brain proteome by large-gel two-dimensional electrophoresis. We detected...
Protein Array Technology: The Tool to Bridge Genomics and Proteomics (2002)
Eickhoff, Holger, Konthur, Zoltán, Lueking, Angelika, Lehrach, Hans, Walter, Gerald, Nordhoff, Eckhard, ...
The generation of protein chips requires much more efforts than DNA microchips. While DNA is DNA and a variety of different DNA molecules behave stable in a hybridisation experiment, proteins are...
By-passing selection: direct screening for antibody–antigen interactions using protein arrays
Holt, Lucy J., Büssow, Konrad, Walter, Gerald, Tomlinson, Ian M.
We have developed a system to identify highly specific antibody–antigen interactions by protein array screening. This removes the need for selection using animal immunisation or in vitro techniques...
Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...
A catalog of human cDNA expression clones and its application to structural genomics
Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...
A systematic approach for identifying human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli is described.
Cepok, Sabine, Zhou, Dun, Srivastava, Rajneesh, Nessler, Stefan, Stei, Susanne, Büssow, Konrad, ...
MS is a chronic inflammatory and demyelinating disease of the CNS with as yet unknown etiology. A hallmark of this disease is the occurrence of oligoclonal IgG antibodies in the cerebrospinal fluid...
Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...
The structure of the TRAPP subunit TPC6 suggests a model for a TRAPP subcomplex
Kümmel, Daniel, Müller, Jürgen J, Roske, Yvette, Misselwitz, Rolf, Büssow, Konrad, Heinemann, Udo
The TRAPP (transport protein particle) complexes are tethering complexes that have an important role at the different steps of vesicle transport. Recently, the crystal structures of the TRAPP...
By-passing selection: direct screening for antibody–antigen interactions using protein arrays
Holt, Lucy J., Büssow, Konrad, Walter, Gerald, Tomlinson, Ian M.
We have developed a system to identify highly specific antibody–antigen interactions by protein array screening. This removes the need for selection using animal immunisation or in vitro techniques...
Scheich, Christoph, Leitner, Dietmar, Sievert, Volker, Leidert, Martina, Schlegel, Brigitte, Simon, Bernd, ...
A catalog of human cDNA expression clones and its application to structural genomics
Büssow, Konrad, Quedenau, Claudia, Sievert, Volker, Tischer, Janett, Scheich, Christoph, Seitz, Harald, ...
A systematic approach for identifying human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli is described.
Cepok, Sabine, Zhou, Dun, Srivastava, Rajneesh, Nessler, Stefan, Stei, Susanne, Büssow, Konrad, ...
MS is a chronic inflammatory and demyelinating disease of the CNS with as yet unknown etiology. A hallmark of this disease is the occurrence of oligoclonal IgG antibodies in the cerebrospinal fluid...
Büssow, Konrad, Scheich, Christoph, Sievert, Volker, Harttig, Ulrich, Schultz, Jörg, Simon, Bernd, ...
X-ray structure of engineered human Aortic Preferentially Expressed Protein-1 (APEG-1)
Manjasetty, Babu A, Niesen, Frank H, Scheich, Christoph, Roske, Yvette, Goetz, Frank, Behlke, Joachim, ...
The structure of the TRAPP subunit TPC6 suggests a model for a TRAPP subcomplex
Kümmel, Daniel, Müller, Jürgen J, Roske, Yvette, Misselwitz, Rolf, Büssow, Konrad, Heinemann, Udo
The TRAPP (transport protein particle) complexes are tethering complexes that have an important role at the different steps of vesicle transport. Recently, the crystal structures of the TRAPP...
Vectors for co-expression of an unrestricted number of proteins
Scheich, Christoph, Kümmel, Daniel, Soumailakakis, Dimitri, Heinemann, Udo, Büssow, Konrad
A vector system is presented that allows generation of E. coli co-expression clones by a standardized, robust cloning procedure. The number of co-expressed proteins is not limited. Five ‘pQLink’...
Homologous high-throughput expression and purification of highly conserved E coli proteins
Ergin, Asgar, Büssow, Konrad, Sieper, Joachim, Thiel, Andreas, Duchmann, Rainer, Adam, Thomas
Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold
Manjasetty, Babu A., Büssow, Konrad, Fieber-Erdmann, Martin, Roske, Yvette, Gobom, Johan, Scheich, Christoph, ...
The human protein PTD012 is the longer product of an alternatively spliced gene and was described to be localized in the nucleus. The X-ray structure analysis at 1.7 Å resolution of PTD012 through...
An automated in vitro protein folding screen applied to a human dynactin subunit
Scheich, Christoph, Niesen, Frank H., Seckler, Robert, Büssow, Konrad
The preparation of proteins for structural and functional analysis using the Escherichia coli expression system is often hampered by the formation of insoluble intracellular protein aggregates...