Koichi Kawakami

Tol2: a versatile gene transfer vector in vertebrates (2007)

Kawakami, Koichi

Abstract The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has...

Targeting neural circuitry in zebrafish using GAL4 enhancer trapping (2007)

Scott, Ethan K., Mason, Lindsay, Arrenberg, Aristides B., Ziv, Limor, Gosse, Nathan J., Xiao, Tong, ...

We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The...

Migration of zebrafish primordial germ cells: a role for myosin contraction and cytoplasmic flow (2006)

Blaser, Heiko, Reichman-Fried, Michal, Castanon, Irinka, Dumstrei, Karin, Marlow, Florence L, Kawakami, Koichi, ...

The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells...

Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage

Kawakami, Koichi, Shima, Akihiro, Kawakami, Noriko

The Tol2 element of the medaka fish Oryzias latipes belongs to the hAT family of transposons (hobo/Ac/Tam3). We report here identification of a functional transposase of Tol2 that is capable of...

The complexity of alternative splicing of hagoromo mRNAs is increased in an explosively speciated lineage in East African cichlids

Terai, Yohey, Morikawa, Naoko, Kawakami, Koichi, Okada, Norihiro

The adaptive radiation of cichlid fishes in the lakes of East Africa is a prime example of speciation. The choice of cichlid mates on the basis of a variety of coloration represents a potential basis...

Fugu ESTs: New Resources for Transcription Analysis and Genome Annotation

Clark, Melody S., Edwards, Yvonne J.K., Peterson, Dan, Clifton, Sandra W., Thompson, Amanda J., Sasaki, Masahide, ...

The draft Fugu rubripes genome was released in 2002, at which time relatively few cDNAs were available to aid in the annotation of genes. The data presented here describe the sequencing and analysis...

The Zebrafish pob Gene Encodes a Novel Protein Required for Survival of Red Cone Photoreceptor Cells

Taylor, Michael R., Kikkawa, Satoshi, Diez-Juan, Antonio, Ramamurthy, Visvanathan, Kawakami, Koichi, Carmeliet, Peter, ...

The zebrafish mutant, partial optokinetic response b (pob), was isolated using an N-ethyl N-nitrosourea (ENU)-based screening strategy designed to identify larvae with defective optokinetic responses...

Transposition of the Tol2 element, an Ac-like element from the Japanese medaka fish Oryzias latipes, in mouse embryonic stem cells.

Kawakami, Koichi, Noda, Tetsuo

The Tol2 transposable element of the Japanese medaka fish belongs to the hAT family of transposons including hobo of Drosophila, Ac of maize, and Tam3 of snapdragon. To date, Tol2 is the only natural...

Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage

Kawakami, Koichi, Shima, Akihiro, Kawakami, Noriko

The Tol2 element of the medaka fish Oryzias latipes belongs to the hAT family of transposons (hobo/Ac/Tam3). We report here identification of a functional transposase of Tol2 that is capable of...

The complexity of alternative splicing of hagoromo mRNAs is increased in an explosively speciated lineage in East African cichlids

Terai, Yohey, Morikawa, Naoko, Kawakami, Koichi, Okada, Norihiro

The adaptive radiation of cichlid fishes in the lakes of East Africa is a prime example of speciation. The choice of cichlid mates on the basis of a variety of coloration represents a potential basis...

Fugu ESTs: New Resources for Transcription Analysis and Genome Annotation

Clark, Melody S., Edwards, Yvonne J.K., Peterson, Dan, Clifton, Sandra W., Thompson, Amanda J., Sasaki, Masahide, ...

The draft Fugu rubripes genome was released in 2002, at which time relatively few cDNAs were available to aid in the annotation of genes. The data presented here describe the sequencing and analysis...

The Zebrafish pob Gene Encodes a Novel Protein Required for Survival of Red Cone Photoreceptor Cells

Taylor, Michael R., Kikkawa, Satoshi, Diez-Juan, Antonio, Ramamurthy, Visvanathan, Kawakami, Koichi, Carmeliet, Peter, ...

The zebrafish mutant, partial optokinetic response b (pob), was isolated using an N-ethyl N-nitrosourea (ENU)-based screening strategy designed to identify larvae with defective optokinetic responses...

Transposition of the Tol2 element, an Ac-like element from the Japanese medaka fish Oryzias latipes, in mouse embryonic stem cells.

Kawakami, Koichi, Noda, Tetsuo

The Tol2 transposable element of the Japanese medaka fish belongs to the hAT family of transposons including hobo of Drosophila, Ac of maize, and Tam3 of snapdragon. To date, Tol2 is the only natural...

Functional Dissection of the Tol2 Transposable Element Identified the Minimal cis-Sequence and a Highly Repetitive Sequence in the Subterminal Region Essential for Transposition

Urasaki, Akihiro, Morvan, Ghislaine, Kawakami, Koichi

The Tol2 element is a naturally occurring active transposable element found in vertebrate genomes. The Tol2 transposon system has been shown to be active from fish to mammals and considered to be a...

A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart

Seguchi, Osamu, Takashima, Seiji, Yamazaki, Satoru, Asakura, Masanori, Asano, Yoshihiro, Shintani, Yasunori, ...

Marked sarcomere disorganization is a well-documented characteristic of cardiomyocytes in the failing human myocardium. Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC2v),...

Tol2: a versatile gene transfer vector in vertebrates

Kawakami, Koichi

The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has 200 and 150...

Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish

Asakawa, Kazuhide, Suster, Maximiliano L., Mizusawa, Kanta, Nagayoshi, Saori, Kotani, Tomoya, Urasaki, Akihiro, ...

Targeted gene expression is a powerful approach to study the function of genes and cells in vivo. In Drosophila, the P element-mediated Gal4-UAS method has been successfully used for this purpose....