Kihoon Kim

Effective inhibition of Rta expression and lytic replication of Kaposi's sarcoma-associated herpesvirus by human RNase P

Zhu, Jiaming, Trang, Phong, Kim, Kihoon, Zhou, Tianhong, Deng, Hongyu, Liu, Fenyong

Ribonuclease P (RNase P) complexed with external guide sequence (EGS) represents a nucleic acid-based gene interference approach to knock-down gene expression. Unlike other strategies, such as...

RNase P ribozyme inhibits cytomegalovirus replication by blocking the expression of viral capsid proteins

Kim, Kihoon, Trang, Phong, Umamoto, Sean, Hai, Rong, Liu, Fenyong

By linking a guide sequence to the catalytic RNA subunit of RNase P (M1 RNA), we constructed a functional ribozyme (M1GS RNA) that targets the overlapping mRNA region of two human cytomegalovirus...

Engineered external guide sequences are highly effective in inducing RNase P for inhibition of gene expression and replication of human cytomegalovirus

Yang, Yong-Hua, Li, Hongjian, Zhou, Tianhong, Kim, Kihoon, Liu, Fenyong

External guide sequences (EGSs), which are RNA molecules derived from natural tRNAs, bind to a target mRNA and render the mRNA susceptible to hydrolysis by RNase P, a tRNA processing enzyme. Using an...

Intracellular expression of engineered RNase P ribozymes effectively blocks gene expression and replication of human cytomegalovirus

KIM, KIHOON, UMAMOTO, SEAN, TRANG, PHONG, HAI, RONG, LIU, FENYONG

A ribozyme (M1GS RNA) constructed from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the overlapping region of two human cytomegalovirus (HCMV) mRNAs, which encode for...

Effective inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external guide sequence RNA

LI, HONGJIAN, TRANG, PHONG, KIM, KIHOON, ZHOU, TIANHONG, UMAMOTO, SEAN, LIU, FENYONG

RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. In this study, a functional EGS RNA was constructed...

Effective inhibition of Rta expression and lytic replication of Kaposi's sarcoma-associated herpesvirus by human RNase P

Zhu, Jiaming, Trang, Phong, Kim, Kihoon, Zhou, Tianhong, Deng, Hongyu, Liu, Fenyong

Ribonuclease P (RNase P) complexed with external guide sequence (EGS) represents a nucleic acid-based gene interference approach to knock-down gene expression. Unlike other strategies, such as...

RNase P ribozyme inhibits cytomegalovirus replication by blocking the expression of viral capsid proteins

Kim, Kihoon, Trang, Phong, Umamoto, Sean, Hai, Rong, Liu, Fenyong

By linking a guide sequence to the catalytic RNA subunit of RNase P (M1 RNA), we constructed a functional ribozyme (M1GS RNA) that targets the overlapping mRNA region of two human cytomegalovirus...

Engineered external guide sequences are highly effective in inducing RNase P for inhibition of gene expression and replication of human cytomegalovirus

Yang, Yong-Hua, Li, Hongjian, Zhou, Tianhong, Kim, Kihoon, Liu, Fenyong

External guide sequences (EGSs), which are RNA molecules derived from natural tRNAs, bind to a target mRNA and render the mRNA susceptible to hydrolysis by RNase P, a tRNA processing enzyme. Using an...

Effective inhibition of human cytomegalovirus gene expression and growth by intracellular expression of external guide sequence RNA

LI, HONGJIAN, TRANG, PHONG, KIM, KIHOON, ZHOU, TIANHONG, UMAMOTO, SEAN, LIU, FENYONG

RNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. In this study, a functional EGS RNA was constructed...

Intracellular expression of engineered RNase P ribozymes effectively blocks gene expression and replication of human cytomegalovirus

KIM, KIHOON, UMAMOTO, SEAN, TRANG, PHONG, HAI, RONG, LIU, FENYONG

A ribozyme (M1GS RNA) constructed from the catalytic RNA subunit of RNase P from Escherichia coli was used to target the overlapping region of two human cytomegalovirus (HCMV) mRNAs, which encode for...