Josefin Fernius, Kevin G. Hardwick
During cell division all chromosomes must be segregated accurately to each daughter cell. Errors in this process give rise to aneuploidy, which leads to birth defects and is implicated in cancer...
Josefin Fernius, Kevin G. Hardwick
During cell division all chromosomes must be segregated accurately to each daughter cell. Errors in this process give rise to aneuploidy, which leads to birth defects and is implicated in cancer...
Chen, Rey-Huei, Brady, D. Michelle, Smith, Dana, Murray, Andrew W., Hardwick, Kevin G.
The spindle checkpoint arrests the cell cycle at metaphase in the presence of defects in the mitotic spindle or in the attachment of chromosomes to the spindle. When spindle assembly is disrupted,...
Distinct Chromosome Segregation Roles for Spindle Checkpoint Proteins
Warren, Cheryl D., Brady, D. Michelle, Johnston, Raymond C., Hanna, Joseph S., Hardwick, Kevin G., Spencer, Forrest A.
The spindle checkpoint plays a central role in the fidelity of chromosome transmission by ensuring that anaphase is initiated only after kinetochore-microtubule associations of all sister chromatid...
Millband, David N., Hardwick, Kevin G.
The spindle checkpoint delays the metaphase-to-anaphase transition in response to spindle and kinetochore defects. Genetic screens in budding yeast identified the Mad and Bub proteins as key...
Vanoosthuyse, Vincent, Valsdottir, Rebekka, Javerzat, Jean-Paul, Hardwick, Kevin G.
Several lines of evidence suggest that kinetochores are organizing centers for the spindle checkpoint response and the synthesis of a “wait anaphase” signal in cases of incomplete or improper...
Kadura, Sheila, He, Xiangwei, Vanoosthuyse, Vincent, Hardwick, Kevin G., Sazer, Shelley
During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby...
Chen, Rey-Huei, Brady, D. Michelle, Smith, Dana, Murray, Andrew W., Hardwick, Kevin G.
The spindle checkpoint arrests the cell cycle at metaphase in the presence of defects in the mitotic spindle or in the attachment of chromosomes to the spindle. When spindle assembly is disrupted,...
Distinct Chromosome Segregation Roles for Spindle Checkpoint Proteins
Warren, Cheryl D., Brady, D. Michelle, Johnston, Raymond C., Hanna, Joseph S., Hardwick, Kevin G., Spencer, Forrest A.
The spindle checkpoint plays a central role in the fidelity of chromosome transmission by ensuring that anaphase is initiated only after kinetochore-microtubule associations of all sister chromatid...
Millband, David N., Hardwick, Kevin G.
The spindle checkpoint delays the metaphase-to-anaphase transition in response to spindle and kinetochore defects. Genetic screens in budding yeast identified the Mad and Bub proteins as key...
Vanoosthuyse, Vincent, Valsdottir, Rebekka, Javerzat, Jean-Paul, Hardwick, Kevin G.
Several lines of evidence suggest that kinetochores are organizing centers for the spindle checkpoint response and the synthesis of a “wait anaphase” signal in cases of incomplete or improper...
Kadura, Sheila, He, Xiangwei, Vanoosthuyse, Vincent, Hardwick, Kevin G., Sazer, Shelley
During mitosis, the spindle assembly checkpoint (SAC) responds to faulty attachments between kinetochores and the mitotic spindle by imposing a metaphase arrest until the defect is corrected, thereby...
Mad3 KEN Boxes Mediate both Cdc20 and Mad3 Turnover, and Are Critical for the Spindle Checkpoint
King, Emma M.J., Hardwick, Kevin G.
Mitotic progression is controlled by proteolytic destruction of securin and cyclin. The mitotic E3 ubiquitin ligase, known as the anaphase promoting complex or cyclosome (APC/C), in partnership with...
Shugoshin 2 Regulates Localization of the Chromosomal Passenger Proteins in Fission Yeast Mitosis
Vanoosthuyse, Vincent, Prykhozhij, Sergey, Hardwick, Kevin G.
Fission yeast has two members of the Shugoshin family, Sgo1 and Sgo2. Although Sgo1 has clearly been established as a protector of centromere cohesion in meiosis I, the roles of Sgo2 remain elusive....
Novel sfi1 Alleles Uncover Additional Functions for Sfi1p in Bipolar Spindle Assembly and Function
Anderson, Victoria E., Prudden, John, Prochnik, Simon, Giddings, Thomas H., Hardwick, Kevin G.
A variety of spindle and kinetochore defects have been shown to induce a mitotic delay through activation of the spindle checkpoint. With the aim of identifying novel mitotic defects we carried out a...
King, Emma M.J., Rachidi, Najma, Morrice, Nick, Hardwick, Kevin G., Stark, Michael J.R.
The spindle checkpoint delays anaphase onset until all chromosomes are correctly attached to microtubules. Ipl1 protein kinase (Aurora B) is required to correct inappropriate...
Bub1 Kinase Targets Sgo1 to Ensure Efficient Chromosome Biorientation in Budding Yeast Mitosis
Fernius, Josefin, Hardwick, Kevin G
During cell division all chromosomes must be segregated accurately to each daughter cell. Errors in this process give rise to aneuploidy, which leads to birth defects and is implicated in cancer...
Rischitor, Patricia E., May, Karen M., Hardwick, Kevin G.
The spindle checkpoint delays anaphase onset until all chromosomes have attached in a bi-polar manner to the mitotic spindle. Mad and Bub proteins are recruited to unattached kinetochores, and...
Hardwick, Kevin G., Johnston, Raymond C., Smith, Dana L., Murray, Andrew W.
We show that MAD3 encodes a novel 58-kD nuclear protein which is not essential for viability, but is an integral component of the spindle checkpoint in budding yeast. Sequence analysis reveals two...
Cdc28 Activates Exit from Mitosis in Budding Yeast
Rudner, Adam D., Hardwick, Kevin G., Murray, Andrew W.
The activity of the cyclin-dependent kinase 1 (Cdk1), Cdc28, inhibits the transition from anaphase to G1 in budding yeast. CDC28-T18V, Y19F (CDC28-VF), a mutant that lacks inhibitory phosphorylation...
Sczaniecka, Matylda, Feoktistova, Anna, May, Karen M., Chen, Jun-Song, Blyth, Julie, Gould, Kathleen L., ...
Mitotic progression is driven by proteolytic destruction of securin and cyclins. These proteins are labeled for destruction by an ubiquitin-protein isopeptide ligase (E3) known as the...