Huang, Shaohui, Lifshitz, Lawrence M., Jones, Christine, Bellve, Karl D., Standley, Clive, Fonseca, Sonya G., ...
Total internal reflection fluorescence (TIRF) microscopy reveals highly mobile structures containing enhanced green fluorescent protein-tagged glucose transporter 4 (GLUT4) within a zone about 100 nm...
Hayakawa, Akira, Leonard, Deborah Marie, Murphy, Stephanie, Hayes, Susan J., Soto, Martha C., Fogarty, Kevin E., ...
The FYVE domain binds with high specificity and avidity to phosphatidylinositol 3-phosphate. It is present in approximately 30 proteins in humans, some of which have been implicated in functions...
De Crescenzo, Valerie, Fogarty, Kevin E., ZhuGe, Ronghua, Tuft, Richard A., Lifshitz, Lawrence M., Carmichael, Jeffrey, ...
Ca2+ stores were studied in a preparation of freshly dissociated terminals from hypothalamic magnocellular neurons. Depolarization from a holding level of -80 mV in the absence of extracellular Ca2+...
Plasma membrane domains specialized for clathrin-mediated endocytosis in primary cells (2006)
Bellve, Karl D., Leonard, Deborah, Standley, Clive, Lifshitz, Lawrence M., Tuft, Richard A., Hayakawa, Akira, ...
Clathrin assembly at the plasma membrane is a fundamental process required for endocytosis. In cultured cells, most of the clathrin is localized to large patches that display little lateral mobility....
Zhuge, Ronghua, DeCrescenzo, Valerie, Sorrentino, Vincenzo, Lai, F. Anthony, Tuft, Richard A., Lifshitz, Lawrence M., ...
Spontaneous, short-lived, focal cytosolic Ca2+ transients were found for the first time and characterized in freshly dissociated chromaffin cells from mouse. Produced by release of Ca2+ from...
ZhuGe, Ronghua, DeCrescenzo, Valerie, Sorrentino, Vincenzo, Lai, F. Anthony, Tuft, Richard A., Lifshitz, Lawrence M., ...
Spontaneous, short-lived, focal cytosolic Ca2+ transients were found for the first time and characterized in freshly dissociated chromaffin cells from mouse. Produced by release of Ca2+ from...
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
The feasibility of determining localized Ca(2+) influx using only wide-field fluorescence images was explored by imaging (using fluo-3) single channel Ca(2+) fluorescence transients (SCCaFTs), due to...
Zhuge, Ronghua, Fogarty, Kevin E., Baker, Stephen P., McCarron, John G., Tuft, Richard A., Lifshitz, Lawrence M., ...
Ca(2+) sparks are highly localized Ca(2+) transients caused by Ca(2+) release from sarcoplasmic reticulum through ryanodine receptors (RyR). In smooth muscle, Ca(2+) sparks activate nearby...
Spontaneous mitochondrial depolarizations are independent of SR Ca2+ release (2004)
O'Reilly, Catherine M., Fogarty, Kevin E., Drummond, Robert M., Tuft, Richard A., Walsh, John V.
The mitochondrial membrane potential (DeltaPsi(m)) underlies many mitochondrial functions, including Ca(2+) influx into the mitochondria, which allows them to serve as buffers of intracellular...
Structural basis for endosomal targeting by FYVE domains (2003)
Hayakawa, Akira, Hayes, Susan J., Lawe, Deirdre C., Sudharshan, Eathiraj, Tuft, Richard A., Fogarty, Kevin E., ...
The FYVE domain is a conserved protein motif characterized by its ability to bind with high affinity and specificity to phosphatidylinositol 3-phosphate (PI3P), a phosphoinositide highly enriched in...
Quantitative analysis of spontaneous mitochondrial depolarizations (2003)
O'Reilly, Catherine M., Fogarty, Kevin E., Drummond, Robert M., Tuft, Richard A., Walsh, John V.
Spontaneous transient depolarizations in mitochondrial membrane potential (DeltaPsi(m)), mitochondrial flickers, have been observed in isolated mitochondria and intact cells using the fluorescent...
Essential role of Ca2+/calmodulin in Early Endosome Antigen-1 localization (2003)
Lawe, Deirdre C., Sitouah, Nachida, Hayes, Susan, Chawla, Anil, Virbasius, Joseph V., Tuft, Richard A., ...
Ca2+ is an essential requirement in membrane fusion, acting through binding proteins such as calmodulin (CaM). Ca2+/CaM is required for early endosome fusion in vitro, however, the molecular basis...
Visualization of single molecules of mRNA in situ (2003)
Femino, Andrea M., Fogarty, Kevin E., Lifshitz, Lawrence M., Carrington, Walter A., Singer, Robert H.
ZhuGe, Ronghua, Fogarty, Kevin E., Tuft, Richard A., Walsh, John V.
Ca(2+) sparks are small, localized cytosolic Ca(2+) transients due to Ca(2+) release from sarcoplasmic reticulum through ryanodine receptors. In smooth muscle, Ca(2+) sparks activate large...
Ballen, Karen K., Heseth, Ann M., Heyes, Christine, Becker, Pamela S., Emmons, Robert V. B., Fogarty, Kevin E., ...
Considerable progress has been made in improving the control of chemotherapy-induced emesis. The impact of available antiemetic options for patients receiving stem cell transplants is unclear, as few...
Kidd, Jackie F., Pilkington, Mary F., Schell, Michael J., Fogarty, Kevin E., Skepper, Jeremy N., Taylor, Colin W., ...
We have characterized the effects of the antimitotic drug paclitaxel (Taxol(TM)) on the Ca(2+) signaling cascade of terminally differentiated mouse pancreatic acinar cells. Using single cell...
Lawe, Deirdre C., Chawla, Anil, Merithew, Eric Lee, Dumas, John J., Carrington, Walter A., Fogarty, Kevin E., ...
Early endosome antigen 1 (EEA1) is a 170-kDa polypeptide required for endosome fusion in mammalian cells. The COOH terminus of EEA1 contains a FYVE domain that interacts specifically with...
ZhuGe, Ronghua, Fogarty, Kevin E., Tuft, Richard A., Lifshitz, Lawrence M., Sayar, Kemal, Walsh, John V.
Ca(2+) sparks are highly localized cytosolic Ca(2+) transients caused by a release of Ca(2+) from the sarcoplasmic reticulum via ryanodine receptors (RyRs); they are the elementary events underlying...
A bimodal pattern of InsP(3)-evoked elementary Ca(2+) signals in pancreatic acinar cells (2000)
Fogarty, Kevin E., Kidd, Jackie F., Tuft, Richard A., Thorn, Peter
InsP(3)-evoked elementary Ca(2+) release events have been postulated to play a role in providing the building blocks of larger Ca(2+) signals. In pancreatic acinar cells, low concentrations of...
Microtubules Regulate Local Ca2+ Spiking in Secretory Epithelial Cells (2000)
Fogarty, Kevin E., Kidd, Jackie F., Turner, Angelina, Skepper, Jeremy N., Carmichael, Jeff, Thorn, Peter
The role of the cytoskeleton in regulating Ca2+ release has been explored in epithelial cells. Trains of local Ca2+ spikes were elicited in pancreatic acinar cells by infusion of inositol...
Mechanisms underlying InsP3-evoked global Ca2+ signals in mouse pancreatic acinar cells (2000)
Fogarty, Kevin E., Kidd, Jackie F., Tuft, Dick A., Thorn, Peter
1. In secretory epithelial cells, complex patterns of Ca2+ signals regulate physiological processes. How these patterns are generated is still not fully understood. In particular, the basis of global...
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
Discrete localized fluorescence transients due to openings of a single plasma membrane Ca(2+) permeable cation channel were recorded using wide-field digital imaging microscopy with fluo-3 as the...
ZhuGe, Ronghua, Tuft, Richard A., Fogarty, Kevin E., Bellve, Karl D., Fay, Fredric S., Walsh, John V.
Localized, transient elevations in cytosolic Ca2+, known as Ca2+ sparks, caused by Ca2+ release from sarcoplasmic reticulum, are thought to trigger the opening of large conductance Ca2+-activated...
Visualization of single RNA transcripts in situ (1998)
Femino, Andrea M., Fay, Fredric S., Fogarty, Kevin E., Singer, Robert H.
Fluorescence in situ hybridization (FISH) and digital imaging microscopy were modified to allow detection of single RNA molecules. Oligodeoxynucleotide probes were synthesized with five fluorochromes...
Chemotaxis of newt eosinophils: calcium regulation of chemotactic response (1993)
Brundage, Rodney A., Fogarty, Kevin E., Tuft, Richard A., Fay, Fredric S.
Local chemical events underlying chemotaxis were characterized in a new model cell, the newt eosinophil. These cells exhibit a chemotactic response to a trypsin-sensitive component of newt serum....
Calcium gradients underlying polarization and chemotaxis of eosinophils (1991)
Brundage, Rodney A., Fogarty, Kevin E., Tuft, Richard A., Fay, Fredric S.
The concentration of intracellular free calcium ([Ca2+]i) in polarized eosinophils was imaged during chemotaxis by monitoring fluorescence of the calcium-sensitive dye Fura-2 with a modified digital...
Lynch, Ronald M., Fogarty, Kevin E., Fay, Fredric S.
Hexokinase isozyme I is proposed to be associated with mitochondria in vivo. Moreover, it has been suggested that this association is modulated in coordination with changes in cell metabolic state....
Intercellular Calcium Waves in HeLa Cells Expressing GFP-labeled Connexin 43, 32, or 26
Paemeleire, Koen, Martin, Patricia E. M., Coleman, Sharon L., Fogarty, Kevin E., Carrington, Walter A., Leybaert, Luc, ...
This study was undertaken to obtain direct evidence for the involvement of gap junctions in the propagation of intercellular Ca2+ waves. Gap junction-deficient HeLa cells were transfected with...
Visualization of Ca2+ entry through single stretch-activated cation channels
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
Stretch-activated channels (SACs) have been found in smooth muscle and are thought to be involved in myogenic responses. Although SACs have been shown to be Ca2+ permeable when Ca2+ is the only...
Thorn, Peter, Fogarty, Kevin E., Parker, Ian
The dynamics of the fusion pore that forms between a secretory vesicle and the plasma membrane are important in the regulation of both exocytosis and endocytosis. Here, we describe characteristics of...
Quantitative Analysis of Spontaneous Mitochondrial Depolarizations
O'Reilly, Catherine M., Fogarty, Kevin E., Drummond, Robert M., Tuft, Richard A., Walsh, John V.
Spontaneous transient depolarizations in mitochondrial membrane potential (ΔΨm), mitochondrial flickers, have been observed in isolated mitochondria and intact cells using the fluorescent probe,...
Rapid, Diffusional Shuttling of Poly(A) RNA between Nuclear Speckles and the NucleoplasmD⃞
Politz, Joan C. Ritland, Tuft, Richard A., Prasanth, Kannanganattu V., Baudendistel, Nina, Fogarty, Kevin E., Lifshitz, Larry M., ...
Speckles are nuclear bodies that contain pre-mRNA splicing factors and polyadenylated RNA. Because nuclear poly(A) RNA consists of both mRNA transcripts and nucleus-restricted RNAs, we tested whether...
Follit, John A., Tuft, Richard A., Fogarty, Kevin E., Pazour, Gregory J.
Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT particles are composed of at least 17 polypeptides...
Intercellular Calcium Waves in HeLa Cells Expressing GFP-labeled Connexin 43, 32, or 26
Paemeleire, Koen, Martin, Patricia E. M., Coleman, Sharon L., Fogarty, Kevin E., Carrington, Walter A., Leybaert, Luc, ...
This study was undertaken to obtain direct evidence for the involvement of gap junctions in the propagation of intercellular Ca2+ waves. Gap junction-deficient HeLa cells were transfected with...
Visualization of Ca2+ entry through single stretch-activated cation channels
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
Stretch-activated channels (SACs) have been found in smooth muscle and are thought to be involved in myogenic responses. Although SACs have been shown to be Ca2+ permeable when Ca2+ is the only...
Thorn, Peter, Fogarty, Kevin E., Parker, Ian
The dynamics of the fusion pore that forms between a secretory vesicle and the plasma membrane are important in the regulation of both exocytosis and endocytosis. Here, we describe characteristics of...
Quantitative Analysis of Spontaneous Mitochondrial Depolarizations
O'Reilly, Catherine M., Fogarty, Kevin E., Drummond, Robert M., Tuft, Richard A., Walsh, John V.
Spontaneous transient depolarizations in mitochondrial membrane potential (ΔΨm), mitochondrial flickers, have been observed in isolated mitochondria and intact cells using the fluorescent probe,...
Rapid, Diffusional Shuttling of Poly(A) RNA between Nuclear Speckles and the NucleoplasmD⃞
Politz, Joan C. Ritland, Tuft, Richard A., Prasanth, Kannanganattu V., Baudendistel, Nina, Fogarty, Kevin E., Lifshitz, Larry M., ...
Speckles are nuclear bodies that contain pre-mRNA splicing factors and polyadenylated RNA. Because nuclear poly(A) RNA consists of both mRNA transcripts and nucleus-restricted RNAs, we tested whether...
Follit, John A., Tuft, Richard A., Fogarty, Kevin E., Pazour, Gregory J.
Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT particles are composed of at least 17 polypeptides...
ZhuGe, Ronghua, DeCrescenzo, Valerie, Sorrentino, Vincenzo, Lai, F. Anthony, Tuft, Richard A., Lifshitz, Lawrence M., ...
Spontaneous, short-lived, focal cytosolic Ca2+ transients were found for the first time and characterized in freshly dissociated chromaffin cells from mouse. Produced by release of Ca2+ from...
Huang, Shaohui, Lifshitz, Larry M., Jones, Christine, Bellve, Karl D., Standley, Clive, Fonseca, Sonya, ...
Total internal reflection fluorescence (TIRF) microscopy reveals highly mobile structures containing enhanced green fluorescent protein-tagged glucose transporter 4 (GLUT4) within a zone about 100 nm...
ZhuGe, Ronghua, Sims, Stephen M, Tuft, Richard A, Fogarty, Kevin E, Walsh, John V
Local changes in cytosolic [Ca2+] were imaged with a wide-field, high-speed, digital imaging system while membrane currents were simultaneously recorded using whole-cell, perforated patch recording...
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
The feasibility of determining localized Ca2+ influx using only wide-field fluorescence images was explored by imaging (using fluo-3) single channel Ca2+ fluorescence transients (SCCaFTs), due to...
Kirber, Michael T, Guerrero-Hernández, Agustín, Bowman, Douglas S, Fogarty, Kevin E, Tuft, Richard A, Singer, Joshua J, ...
A digital imaging microscope with fura-2 as the Ca2+ indicator was used to determine the sources for the rise in intracellular calcium concentration ([Ca2+]i) that occurs when the membrane in a...
Mechanisms underlying InsP3-evoked global Ca2+ signals in mouse pancreatic acinar cells
Fogarty, Kevin E, Kidd, Jackie F, Tuft, Dick A, Thorn, Peter
In secretory epithelial cells, complex patterns of Ca2+ signals regulate physiological processes. How these patterns are generated is still not fully understood. In particular, the basis of global...
Kirber, Michael T, Etter, Elaine F, Bellvé, Karl A, Lifshitz, Lawrence M, Tuft, Richard A, Fay, Fredric S, ...
We recorded Ca2+ sparks and spontaneous transient outward currents (STOCs) simultaneously in smooth muscle cells using whole-cell patch recording and a unique, high-speed widefield digital imaging...
ZhuGe, Ronghua, Tuft, Richard A., Fogarty, Kevin E., Bellve, Karl, Fay, Fredric S., Walsh, John V.
Localized, transient elevations in cytosolic Ca2+, known as Ca2+ sparks, caused by Ca2+ release from sarcoplasmic reticulum, are thought to trigger the opening of large conductance Ca2+-activated...
Imaging Ca2+ Entering the Cytoplasm through a Single Opening of a Plasma Membrane Cation Channel
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
Discrete localized fluorescence transients due to openings of a single plasma membrane Ca2+ permeable cation channel were recorded using wide-field digital imaging microscopy with fluo-3 as the Ca2+...
ZhuGe, Ronghua, Fogarty, Kevin E., Tuft, Richard A., Walsh, John V.
Ca2+ sparks are small, localized cytosolic Ca2+ transients due to Ca2+ release from sarcoplasmic reticulum through ryanodine receptors. In smooth muscle, Ca2+ sparks activate large conductance...
ZhuGe, Ronghua, Fogarty, Kevin E., Tuft, Richard A., Lifshitz, Lawrence M., Sayar, Kemal, Walsh, John V.
Ca2+ sparks are highly localized cytosolic Ca2+ transients caused by a release of Ca2+ from the sarcoplasmic reticulum via ryanodine receptors (RyRs); they are the elementary events underlying global...
Rapizzi, Elena, Pinton, Paolo, Szabadkai, György, Wieckowski, Mariusz R., Vandecasteele, Grégoire, Baird, Geoff, ...
Although the physiological relevance of mitochondrial Ca2+ homeostasis is widely accepted, no information is yet available on the molecular identity of the proteins involved in this process. Here we...
Development and Application of a Three-Dimensional Artificial Visual System
Coggins, James M., Fogarty, Kevin E., Fay, Frederic S.
A three-dimensional artificial visual system has been developed to aid in the analysis of three-dimensional fluorescence images of smooth muscle cells. The artificial visual system consists of three...