Kent L. McDonald

The C. elegans Opa1 Homologue EAT-3 is Essential for Resistance to Free Radicals (2008)

Takayuki Kanazawa, Mauro Zappaterra, Ayako Hasegawa, Ashley P Wright, Karolyn F Buttle, ...

The most prevalent form of hereditary blindness, Dominant Optic Atrophy, is caused by mutations in the mitochondrial dynamin family member Opa1. Mutations in Opa1 and a homologous protein in yeast...

Autophagy Counterbalances Endoplasmic Reticulum Expansion during the Unfolded Protein Response (2006)

Sebastián Bernales, Kent L. McDonald, Peter Walter

The protein folding capacity of the endoplasmic reticulum (ER) is regulated by the unfolded protein response (UPR). The UPR senses unfolded proteins in the ER lumen and transmits that information to...

Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans. (2003)

O'Toole, Eileen T, McDonald, Kent L, Mantler, Jana, McIntosh, J Richard, Hyman, Anthony A, Muller-Reichert, Thomas

During mitosis, the connections of microtubules (MTs) to centrosomes and kinetochores are dynamic. From in vitro studies, it is known that the dynamic behavior of MTs is related to the structure of...

Dynamic Localization of the Swe1 Regulator Hsl7 During the Saccharomyces cerevisiae Cell Cycle

Cid, Victor J., Shulewitz, Mark J., McDonald, Kent L., Thorner, Jeremy

In Saccharomyces cerevisiae, entry into mitosis requires activation of the cyclin-dependent kinase Cdc28 in its cyclin B (Clb)-associated form. Clb-bound Cdc28 is susceptible to inhibitory tyrosine...

Rapid Translocation of Polarized MDCK Cell Monolayers by Leptospira interrogans, an Invasive but Nonintracellular Pathogen

Barocchi, Michele A., Ko, Albert I., Galvão Reis, Mitermayer, McDonald, Kent L., Riley, Lee W.

Pathogenic spirochetes of the genus Leptospira are a major cause of human zoonotic infectious disease worldwide. After gaining entry through the skin, the organism causes disease by hematogenously...

The coronin-like protein POD-1 is required for anterior–posterior axis formation and cellular architecture in the nematode Caenorhabditis elegans

Rappleye, Chad A., Paredez, Alexander R., Smith, Carol W., McDonald, Kent L., Aroian, Raffi V.

Establishment of anterior–posterior (a-p) polarity in the Caenorhabditis elegans embryo depends on filamentous (F-) actin. Previously, we isolated an F-actin-binding protein that was enriched in...

Spindle Pole Body Duplication in Fission Yeast Occurs at the G1/S Boundary but Maturation Is Blocked until Exit from S by an Event Downstream of Cdc10+D⃞V⃞

Uzawa, Satoru, Li, Fei, Jin, Ye, McDonald, Kent L., Braunfeld, Michael B., Agard, David A., ...

The regulation and timing of spindle pole body (SPB) duplication and maturation in fission yeast was examined by transmission electron microscopy. When cells are arrested at G1 by nitrogen...

Dynamic Localization of the Swe1 Regulator Hsl7 During the Saccharomyces cerevisiae Cell Cycle

Cid, Victor J., Shulewitz, Mark J., McDonald, Kent L., Thorner, Jeremy

In Saccharomyces cerevisiae, entry into mitosis requires activation of the cyclin-dependent kinase Cdc28 in its cyclin B (Clb)-associated form. Clb-bound Cdc28 is susceptible to inhibitory tyrosine...

Rapid Translocation of Polarized MDCK Cell Monolayers by Leptospira interrogans, an Invasive but Nonintracellular Pathogen

Barocchi, Michele A., Ko, Albert I., Galvão Reis, Mitermayer, McDonald, Kent L., Riley, Lee W.

Pathogenic spirochetes of the genus Leptospira are a major cause of human zoonotic infectious disease worldwide. After gaining entry through the skin, the organism causes disease by hematogenously...

The coronin-like protein POD-1 is required for anterior–posterior axis formation and cellular architecture in the nematode Caenorhabditis elegans

Rappleye, Chad A., Paredez, Alexander R., Smith, Carol W., McDonald, Kent L., Aroian, Raffi V.

Establishment of anterior–posterior (a-p) polarity in the Caenorhabditis elegans embryo depends on filamentous (F-) actin. Previously, we isolated an F-actin-binding protein that was enriched in...

Spindle Pole Body Duplication in Fission Yeast Occurs at the G1/S Boundary but Maturation Is Blocked until Exit from S by an Event Downstream of Cdc10+D⃞V⃞

Uzawa, Satoru, Li, Fei, Jin, Ye, McDonald, Kent L., Braunfeld, Michael B., Agard, David A., ...

The regulation and timing of spindle pole body (SPB) duplication and maturation in fission yeast was examined by transmission electron microscopy. When cells are arrested at G1 by nitrogen...

Autophagy Counterbalances Endoplasmic Reticulum Expansion during the Unfolded Protein Response

Bernales, Sebastián, McDonald, Kent L, Walter, Peter

The protein folding capacity of the endoplasmic reticulum (ER) is regulated by the unfolded protein response (UPR). The UPR senses unfolded proteins in the ER lumen and transmits that information to...

Functional modulation of IFT kinesins extends the sensory repertoire of ciliated neurons in Caenorhabditis elegans

Evans, James E., Snow, Joshua J., Gunnarson, Amy L., Ou, Guangshuo, Stahlberg, Henning, McDonald, Kent L., ...

The diversity of sensory cilia on Caenorhabditis elegans neurons allows the animal to detect a variety of sensory stimuli. Sensory cilia are assembled by intraflagellar transport (IFT) kinesins,...

Actin dynamics coupled to clathrin-coated vesicle formation at the trans-Golgi network

Carreno, Sebastien, Engqvist-Goldstein, Åsa E., Zhang, Claire X., McDonald, Kent L., Drubin, David G.

In diverse species, actin assembly facilitates clathrin-coated vesicle (CCV) formation during endocytosis. This role might be an adaptation specific to the unique environment at the cell cortex, or...

Mycobacterium marinum Escapes from Phagosomes and Is Propelled by Actin-based Motility

Stamm, Luisa M., Morisaki, J. Hiroshi, Gao, Lian-Yong, Jeng, Robert L., McDonald, Kent L., Roth, Robyn, ...

Mycobacteria are responsible for a number of human and animal diseases and are classical intracellular pathogens, living inside macrophages rather than as free-living organisms during infection....

Saccharomyces cerevisiae Duo1p and Dam1p, Novel Proteins Involved in Mitotic Spindle Function

Hofmann, Christian, Cheeseman, Iain M., Goode, Bruce L., McDonald, Kent L., Barnes, Georjana, Drubin, David G.

In this paper, we describe the identification and characterization of two novel and essential mitotic spindle proteins, Duo1p and Dam1p. Duo1p was isolated because its overexpression caused defects...

The Bipolar Kinesin, KLP61F, Cross-links Microtubules within Interpolar Microtubule Bundles of Drosophila Embryonic Mitotic Spindles

Sharp, David J., McDonald, Kent L., Brown, Heather M., Matthies, Heinrich J., Walczak, Claire, Vale, Ron D., ...

Previous genetic and biochemical studies have led to the hypothesis that the essential mitotic bipolar kinesin, KLP61F, cross-links and slides microtubules (MTs) during spindle assembly and function....

Dynamic phosphoregulation of the cortical actin cytoskeleton and endocytic machinery revealed by real-time chemical genetic analysis

Sekiya-Kawasaki, Mariko, Groen, Aaron Chris, Kaksonen, Marko, Watson, Hadiya A., Zhang, Chao, ...

We used chemical genetics to control the activity of budding yeast Prk1p, which is a protein kinase that is related to mammalian GAK and AAK1, and which targets several actin regulatory proteins...

Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans

O'Toole, Eileen T., McDonald, Kent L., Mäntler, Jana, McIntosh, J. Richard, Hyman, Anthony A., Müller-Reichert, Thomas

During mitosis, the connections of microtubules (MTs) to centrosomes and kinetochores are dynamic. From in vitro studies, it is known that the dynamic behavior of MTs is related to the structure of...

Him-10 Is Required for Kinetochore Structure and Function on Caenorhabditis elegans Holocentric Chromosomes

Howe, Mary, McDonald, Kent L., Albertson, Donna G., Meyer, Barbara J.

Macromolecular structures called kinetochores attach and move chromosomes within the spindle during chromosome segregation. Using electron microscopy, we identified a structure on the holocentric...