Kensaku Sakamoto

Improving Sequence Design for DNA Computing (2001)

Masanori Arita, Akio Nishikawa, Masami Hagiya, Ken Komiya, Hidetaka Gouzu, Kensaku Sakamoto

Good design of sequences is a necessity for successful DNA computing, but how to achieve this `goodness' is unknown. We point out the importance of adjusting the composition of sequences to fit each...

Towards Parallel Evaluation and Learning of Boolean (2001)

Masami Hagiya, Masanori Arita, Daisuke Kiga, Kensaku Sakamoto

. A --formula is a Boolean formula in which each variable occurs at most once. The paper treats its molecular representation including its queries using techniques in molecular biology. The novelty...

State Transitions by Molecules (1998)

Kensaku Sakamoto, Daisuke Kiga, Ken Komiya, Hidetaka Gouzu, Shigeyuki Yokoyama, Shuji Ikeda, ...

In our previous paper, we described a method by which a state machine is implemented by a single-stranded DNA molecule whose 3 0 -end sequence encodes the current state of the machine. Successive...

State Transitions by Molecules (1998)

Kensaku Sakamoto, Daisuke Kiga, Ken Komiya, Hidetaka Gouzu, Shigeyuki Yokoyama, Shuji Ikeda, ...

In our previous paper, we described a method by which a state machine is implemented by a single-stranded DNA molecule whose 3 0 -end sequence encodes the current state of the machine. Successive...

Towards Parallel Evaluation and Learning of Boolean (1998)

Masami Hagiya, Masanori Arita, Daisuke Kiga, Kensaku Sakamoto

. A ¯--formula is a Boolean formula in which each variable occurs at most once. The paper treats its molecular representation including its queries using techniques in molecular biology. The novelty...

Towards Parallel Evaluation and Learning of Boolean ...-Formulas with Molecules (1997)

Masami Hagiya, Masanori Arita, Daisuke Kiga, Kensaku Sakamoto, Shigeyuki Yokoyama

A ¯--formula is a Boolean formula in which each variable occurs at most once. The paper treats its molecular representation including its queries using techniques in molecular biology. The novelty...

An engineered Escherichia coli tyrosyl–tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

Kiga, Daisuke, Sakamoto, Kensaku, Kodama, Koichiro, Kigawa, Takanori, Matsuda, Takayoshi, Yabuki, Takashi, ...

Tyrosyl–tRNA synthetase (TyrRS) from Escherichia coli was engineered to preferentially recognize 3-iodo-l-tyrosine rather than l-tyrosine for the site-specific incorporation of 3-iodo-l-tyrosine...

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells

Sakamoto, Kensaku, Hayashi, Akiko, Sakamoto, Ayako, Kiga, Daisuke, Nakayama, Hiroshi, Soma, Akiko, ...

A suppressor tRNATyr and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-l-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNATyr...

The Escherichia coli argU10(Ts) Phenotype Is Caused by a Reduction in the Cellular Level of the argU tRNA for the Rare Codons AGA and AGG

Sakamoto, Kensaku, Ishimaru, Satoshi, Kobayashi, Takatsugu, Walker, James R., Yokoyama, Shigeyuki

The Escherichia coli argU10(Ts) mutation in the argU gene, encoding the minor tRNAArg species for the rare codons AGA and AGG, causes pleiotropic defects, including growth inhibition at high...

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion

Kobayashi, Takatsugu, Sakamoto, Kensaku, Takimura, Tetsuo, Sekine, Ryo, Vincent, Kelly, Kamata, Kenji, ...

The genetic code in a eukaryotic system has been expanded by the engineering of Escherichia coli tyrosyl-tRNA synthetase (TyrRS) with the Y37V and Q195C mutations (37V195C), which specifically...

Solution structure of an RNA fragment with the P7/P9.0 region and the 3'-terminal guanosine of the tetrahymena group I intron.

Kitamura, Aya, Muto, Yutaka, Watanabe, Satoru, Kim, Insil, Ito, Takuhiro, Nishiya, Yoichi, ...

In the second step of the two consecutive transesterifications of the self-splicing reaction of the group I intron, the conserved guanosine at the 3' terminus of the intron (omegaG) binds to the...

An engineered Escherichia coli tyrosyl–tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

Kiga, Daisuke, Sakamoto, Kensaku, Kodama, Koichiro, Kigawa, Takanori, Matsuda, Takayoshi, Yabuki, Takashi, ...

Tyrosyl–tRNA synthetase (TyrRS) from Escherichia coli was engineered to preferentially recognize 3-iodo-l-tyrosine rather than l-tyrosine for the site-specific incorporation of 3-iodo-l-tyrosine...

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells

Sakamoto, Kensaku, Hayashi, Akiko, Sakamoto, Ayako, Kiga, Daisuke, Nakayama, Hiroshi, Soma, Akiko, ...

A suppressor tRNATyr and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-l-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNATyr...

The Escherichia coli argU10(Ts) Phenotype Is Caused by a Reduction in the Cellular Level of the argU tRNA for the Rare Codons AGA and AGG

Sakamoto, Kensaku, Ishimaru, Satoshi, Kobayashi, Takatsugu, Walker, James R., Yokoyama, Shigeyuki

The Escherichia coli argU10(Ts) mutation in the argU gene, encoding the minor tRNAArg species for the rare codons AGA and AGG, causes pleiotropic defects, including growth inhibition at high...

Structural basis of nonnatural amino acid recognition by an engineered aminoacyl-tRNA synthetase for genetic code expansion

Kobayashi, Takatsugu, Sakamoto, Kensaku, Takimura, Tetsuo, Sekine, Ryo, Vincent, Kelly, Kamata, Kenji, ...

The genetic code in a eukaryotic system has been expanded by the engineering of Escherichia coli tyrosyl-tRNA synthetase (TyrRS) with the Y37V and Q195C mutations (37V195C), which specifically...

Solution structure of an RNA fragment with the P7/P9.0 region and the 3'-terminal guanosine of the tetrahymena group I intron.

Kitamura, Aya, Muto, Yutaka, Watanabe, Satoru, Kim, Insil, Ito, Takuhiro, Nishiya, Yoichi, ...

In the second step of the two consecutive transesterifications of the self-splicing reaction of the group I intron, the conserved guanosine at the 3' terminus of the intron (omegaG) binds to the...