Keiko Hirose

The dynamin A ring complex: molecular organisation and nucleotide-dependent conformational changes (2002)

Klockow,Boris, Tichelaar,Willem, Madden,Dean R., Niemann,Hartmut H., Akiba,Toshihiko, Hirose,Keiko, ...

Here we show that Dictyostelium discoideum dynamin A is a fast GTPase, binds to negatively charged lipids, and self-assembles into rings and helices in a nucleotide-dependent manner, similar to human...

The dynamin A ring complex: molecular organisation and nucleotide-dependent conformational changes (2002)

Klockow, Boris, Tichelaar, Willem, Madden, Dean R., Niemann, Hartmut H., Akiba, Toshihiko, Hirose, Keiko, ...

Here we show that Dictyostelium discoideum dynamin A is a fast GTPase, binds to negatively charged lipids, and self-assembles into rings and helices in a nucleotide-dependent manner, similar to human...

Congruent Docking of Dimeric Kinesin and ncd into Three-dimensional Electron Cryomicroscopy Maps of Microtubule–Motor ADP Complexes

Hirose, Keiko, Löwe, Jan, Alonso, Maria, Cross, Robert A., Amos, Linda A.

We present a new map showing dimeric kinesin bound to microtubules in the presence of ADP that was obtained by electron cryomicroscopy and image reconstruction. The directly bound monomer (first...

The dynamin A ring complex: molecular organization and nucleotide-dependent conformational changes

Klockow, Boris, Tichelaar, Willem, Madden, Dean R., Niemann, Hartmut H., Akiba, Toshihiko, Hirose, Keiko, ...

Here we show that Dictyostelium discoideum dynamin A is a fast GTPase, binds to negatively charged lipids, and self-assembles into rings and helices in a nucleotide-dependent manner, similar to human...

Coordination of kinesin's two heads studied with mutant heterodimers

Kaseda, Kuniyoshi, Higuchi, Hideo, Hirose, Keiko

A conventional kinesin molecule has two identical catalytic domains (heads) and is thought to use them alternately to move processively, with 8-nm steps. To clarify how each head contributes to the...

Structural comparison of dimeric Eg5, Neurospora kinesin (Nkin) and Ncd head–Nkin neck chimera with conventional kinesin

Hirose, Keiko, Henningsen, Ulrike, Schliwa, Manfred, Toyoshima, Chikashi, Shimizu, Takashi, Alonso, Maria, ...

Cryo-electron microscopy and 3D image reconstruction of microtubules saturated with kinesin dimers has shown one head bound to tubulin, the other free. The free head of rat kinesin sits on the top...

Murine autoimmune hearing loss mediated by CD4+ T cells specific for inner ear peptides

Solares, C. Arturo, Edling, Andrea E., Johnson, Justin M., Baek, Moo-Jin, Hirose, Keiko, Hughes, Gordon B., ...

Autoimmune sensorineural hearing loss (ASNHL) is characterized typically by bilateral, rapidly progressive hearing loss that responds therapeutically to corticosteroid treatment. Despite its name,...

A Bidirectional Kinesin Motor in Live Drosophila Embryos

Sciambi, Catherine J., Komma, Donald J., Sköld, Helén Nilsson, Hirose, Keiko, Endow, Sharyn A.

Spindle assembly and elongation involve poleward and away-from-the-pole forces produced by microtubule dynamics and spindle-associated motors. Here, we show that a bidirectional Drosophila Kinesin-14...

A role of the double-stranded RNA-binding protein PACT in mouse ear development and hearing

Rowe, Theresa M., Rizzi, Mark, Hirose, Keiko, Peters, Gregory A., Sen, Ganes C.

To determine the physiological functions of the mammalian double-stranded RNA-binding protein PACT, the single-copy mouse Pact gene was disrupted and expression of the protein was completely ablated....

Congruent Docking of Dimeric Kinesin and ncd into Three-dimensional Electron Cryomicroscopy Maps of Microtubule–Motor ADP Complexes

Hirose, Keiko, Löwe, Jan, Alonso, Maria, Cross, Robert A., Amos, Linda A.

We present a new map showing dimeric kinesin bound to microtubules in the presence of ADP that was obtained by electron cryomicroscopy and image reconstruction. The directly bound monomer (first...

The dynamin A ring complex: molecular organization and nucleotide-dependent conformational changes

Klockow, Boris, Tichelaar, Willem, Madden, Dean R., Niemann, Hartmut H., Akiba, Toshihiko, Hirose, Keiko, ...

Here we show that Dictyostelium discoideum dynamin A is a fast GTPase, binds to negatively charged lipids, and self-assembles into rings and helices in a nucleotide-dependent manner, similar to human...

Coordination of kinesin's two heads studied with mutant heterodimers

Kaseda, Kuniyoshi, Higuchi, Hideo, Hirose, Keiko

A conventional kinesin molecule has two identical catalytic domains (heads) and is thought to use them alternately to move processively, with 8-nm steps. To clarify how each head contributes to the...

Structural comparison of dimeric Eg5, Neurospora kinesin (Nkin) and Ncd head–Nkin neck chimera with conventional kinesin

Hirose, Keiko, Henningsen, Ulrike, Schliwa, Manfred, Toyoshima, Chikashi, Shimizu, Takashi, Alonso, Maria, ...

Cryo-electron microscopy and 3D image reconstruction of microtubules saturated with kinesin dimers has shown one head bound to tubulin, the other free. The free head of rat kinesin sits on the top...

Murine autoimmune hearing loss mediated by CD4+ T cells specific for inner ear peptides

Solares, C. Arturo, Edling, Andrea E., Johnson, Justin M., Baek, Moo-Jin, Hirose, Keiko, Hughes, Gordon B., ...

Autoimmune sensorineural hearing loss (ASNHL) is characterized typically by bilateral, rapidly progressive hearing loss that responds therapeutically to corticosteroid treatment. Despite its name,...

A role of the double-stranded RNA-binding protein PACT in mouse ear development and hearing

Rowe, Theresa M., Rizzi, Mark, Hirose, Keiko, Peters, Gregory A., Sen, Ganes C.

To determine the physiological functions of the mammalian double-stranded RNA-binding protein PACT, the single-copy mouse Pact gene was disrupted and expression of the protein was completely ablated....

Orphan Glutamate Receptor δ1 Subunit Required for High-Frequency Hearing▿

Gao, Jiangang, Maison, Stéphane F., Wu, Xudong, Hirose, Keiko, Jones, Sherri M., Bayazitov, Ildar, ...

The function of the orphan glutamate receptor delta subunits (GluRδ1 and GluRδ2) remains unclear. GluRδ2 is expressed exclusively in the Purkinje cells of the cerebellum, and GluRδ1 is...

CC Chemokine Receptor 2 is Protective Against Noise-Induced Hair Cell Death: Studies in CX3CR1+/GFP Mice

Sautter, Nathan B., Shick, Elizabeth H., Ransohoff, Richard M., Charo, Israel F., Hirose, Keiko

Acoustic trauma was recently shown to induce an inflammatory response in the ear characterized by rapid entry of macrophages in the spiral ligament. The current study seeks to elucidate the...