Karina Deinert

Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p

Hellmuth, Klaus, Grosjean, Henri, Motorin, Yuri, Deinert, Karina, Hurt, Ed, Simos, George

Saccharomyces cerevisiae cells that carry deletions in both the LOS1 (a tRNA export receptor) and the PUS1 (a tRNA:pseudouridine synthase) genes exhibit a thermosensitive growth defect. A...

The intracellular location of two aminoacyl-tRNA synthetases depends on complex formation with Arc1p

Galani, Kyriaki, Großhans, Helge, Deinert, Karina, Hurt, Eduard C., Simos, George

In yeast, two aminoacyl-tRNA synthetases, MetRS and GluRS, are associated with Arc1p. We have studied the mechanism of this complex formation and found that the non-catalytic N-terminally appended...

Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p

Hellmuth, Klaus, Grosjean, Henri, Motorin, Yuri, Deinert, Karina, Hurt, Ed, Simos, George

Saccharomyces cerevisiae cells that carry deletions in both the LOS1 (a tRNA export receptor) and the PUS1 (a tRNA:pseudouridine synthase) genes exhibit a thermosensitive growth defect. A...

The intracellular location of two aminoacyl-tRNA synthetases depends on complex formation with Arc1p

Galani, Kyriaki, Großhans, Helge, Deinert, Karina, Hurt, Eduard C., Simos, George

In yeast, two aminoacyl-tRNA synthetases, MetRS and GluRS, are associated with Arc1p. We have studied the mechanism of this complex formation and found that the non-catalytic N-terminally appended...

Biogenesis of the Signal Recognition Particle (Srp) Involves Import of Srp Proteins into the Nucleolus, Assembly with the Srp-Rna, and Xpo1p-Mediated Export

Grosshans, Helge, Deinert, Karina, Hurt, Ed, Simos, George

The signal recognition particle (SRP) targets nascent secretory proteins to the ER, but how and where the SRP assembles is largely unknown. Here we analyze the biogenesis of yeast SRP, which consists...