Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
The feasibility of determining localized Ca(2+) influx using only wide-field fluorescence images was explored by imaging (using fluo-3) single channel Ca(2+) fluorescence transients (SCCaFTs), due to...
Clarke, Alison L., Petrou, Steven, Walsh, John V., Singer, Joshua J.
Fatty acids and other negatively charged single-chain lipids increase large-conductance Ca(2+)-activated K(+) (BK(Ca)) channel activity, whereas sphingosine and other positively charged single-chain...
Clarke, Alison L., Petrou, Steven, Walsh, John V., Singer, Joshua J.
To determine the mechanism of fatty acid modulation of rabbit pulmonary artery large-conductance Ca2+ -activated K+ (BK(Ca)) channel activity, we studied effects of fatty acids and other lipids on...
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
Discrete localized fluorescence transients due to openings of a single plasma membrane Ca(2+) permeable cation channel were recorded using wide-field digital imaging microscopy with fluo-3 as the...
Guerrero, Agustin, Singer, Joshua J., Fay, Fredric S.
Activation of ryanodine receptors on the sarcoplasmic reticulum of single smooth muscle cells from the stomach muscularis of Bufo marinus by caffeine is accompanied by a rise in cytoplasmic [Ca2+]...
Caffeine activates a Ca(2+)-permeable, nonselective cation channel in smooth muscle cells (1994)
Guerrero, Agustin, Fay, Fredric S., Singer, Joshua J.
The effects of caffeine on cytoplasmic [Ca2+] ([Ca2+]i) and plasma membrane currents were studied in single gastric smooth muscle cells dissociated from the toad, Bufo marinus. Experiments were...
Kirber, Michael T., Ordway, Richard W., Clapp, Lucie H., Walsh, John V., Singer, Joshua J.
Large conductance Ca(2+)-activated K+ channels in rabbit pulmonary artery smooth muscle cells are activated by membrane stretch and by arachidonic acid and other fatty acids. Activation by stretch...
Hyperpolarization-activated cationic channels in smooth muscle cells are stretch sensitive (1991)
Hisada, Tetsuya, Ordway, Richard W., Kirber, Michael T., Singer, Joshua J., Walsh, John V.
The properties of hyperpolarization-activated channels were studied in single smooth muscle cells from the stomach of the toad, Bufo marinus, using the patch-clamp technique. In cell-attached...
Kirber, Michael T., Ordway, Richard W., Clapp, Lucie H., Sims, Stephen M., Walsh, John V., Singer, Joshua J.
Vivaudou, Michel B., Clapp, Lucie H., Walsh, John V., Singer, Joshua J.
Electrophysiological recordings from freshly dissociated smooth muscle cells from the stomach of the toad Bufo marinus revealed two types of Ca2+ currents. One has a low threshold of activation and...
Visualization of Ca2+ entry through single stretch-activated cation channels
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
Stretch-activated channels (SACs) have been found in smooth muscle and are thought to be involved in myogenic responses. Although SACs have been shown to be Ca2+ permeable when Ca2+ is the only...
EVIDENCE FOR A ROLE OF CYCLIC AMP IN NEUROMUSCULAR TRANSMISSION
Goldberg, Alfred L., Singer, Joshua J.
Experiments were undertaken to determine if the effects of epinephrine in promoting neuromuscular transmission were mediated by adenosine 3′:5′-cyclic phosphate (cyclic AMP). Dibutyryl cyclic AMP...
Visualization of Ca2+ entry through single stretch-activated cation channels
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
Stretch-activated channels (SACs) have been found in smooth muscle and are thought to be involved in myogenic responses. Although SACs have been shown to be Ca2+ permeable when Ca2+ is the only...
EVIDENCE FOR A ROLE OF CYCLIC AMP IN NEUROMUSCULAR TRANSMISSION
Goldberg, Alfred L., Singer, Joshua J.
Experiments were undertaken to determine if the effects of epinephrine in promoting neuromuscular transmission were mediated by adenosine 3′:5′-cyclic phosphate (cyclic AMP). Dibutyryl cyclic AMP...
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
The feasibility of determining localized Ca2+ influx using only wide-field fluorescence images was explored by imaging (using fluo-3) single channel Ca2+ fluorescence transients (SCCaFTs), due to...
Kirber, Michael T, Guerrero-Hernández, Agustín, Bowman, Douglas S, Fogarty, Kevin E, Tuft, Richard A, Singer, Joshua J, ...
A digital imaging microscope with fura-2 as the Ca2+ indicator was used to determine the sources for the rise in intracellular calcium concentration ([Ca2+]i) that occurs when the membrane in a...
Zou, Hui, Ugur, Mehmet, Drummond, Robert M, Singer, Joshua J
Extracellular application of ATP generates two whole-cell currents in toad gastric smooth muscle cells: an immediate inward non-selective cation current (due to the activation of a P2X or P2Z-like...
Dopico, Alejandro M., Walsh, John V., Singer, Joshua J.
Bile acids have been reported to produce relaxation of smooth muscle both in vitro and in vivo. The cellular mechanisms underlying bile acid–induced relaxation are largely unknown. Here we...
Imaging Ca2+ Entering the Cytoplasm through a Single Opening of a Plasma Membrane Cation Channel
Zou, Hui, Lifshitz, Lawrence M., Tuft, Richard A., Fogarty, Kevin E., Singer, Joshua J.
Discrete localized fluorescence transients due to openings of a single plasma membrane Ca2+ permeable cation channel were recorded using wide-field digital imaging microscopy with fluo-3 as the Ca2+...