Hsueh, Robert C, Hammill, Adrienne M, Lee, Jamie A, Uhr, Jonathan W, Scheuermann, Richard H
Abstract Background Immature B lymphocytes and certain B cell lymphomas undergo apoptotic cell death following activation of the B cell antigen receptor (BCR) signal transduction pathway. Several...
Partial characterization of Ia antigens from murine lymphoid cells (1974)
Vitetta, Ellen S., Klein, Jan, Uhr, Jonathan W.
Congenic anti-Ia antisera were used to bind radiolabelled Ia antigens from cells of various strains of mice of known H-2 haplotype. The results indicate that Ia antigens are proteins of molecular...
Marches, Radu, Hsueh, Robert, Uhr, Jonathan W.
The p21WAF1 (p21) cyclin-dependent kinase inhibitor plays a major role in regulating cell cycle arrest. It was recently reported that the p53-independent elevation of p21 protein levels is essential...
Detection and characterization of carcinoma cells in the blood
Racila, Emilian, Euhus, David, Weiss, Arthur J., Rao, Chandra, McConnell, John, ...
A highly sensitive assay combining immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis has been developed to detect, enumerate, and characterize carcinoma...
Ghetie, Maria-Ana, Podar, Erika M., Ilgen, Amy, Gordon, Brian E., Uhr, Jonathan W., Vitetta, Ellen S.
Monoclonal antibodies (mAbs) that exert antitumor activity can do so by virtue of their effector function and/or their ability to signal growth arrest or cell death. In this study, we demonstrate...
A role for intracellular pH in membrane IgM-mediated cell death of human B lymphomas
Marches, Radu, Vitetta, Ellen S., Uhr, Jonathan W.
We show that anti-IgM-induced cell death in a human B lymphoma cell line, B104, is associated with early intracellular acidification and cell shrinkage. In contrast, another human B cell lymphoma...
Uhr, Jonathan W., Schenkein, Isaac
P3K cells were uniformly labeled with [14C]leucine and also for 1 hr with [3H]leucine, [3]galactose, or [3H]glucosamine. Subcellular fractionation of labeled cells was performed and the amounts of...
Sherr, Charles J., Uhr, Jonathan W.
Mouse myeloma cells were pulse-labeled in vitro with 3H-D-glucosamine and 14C-L-leucine. Analysis on sucrose gradients revealed incorporation of both isotopes into polyribosomes and release of most...
Specific removal of in vivo antibody by extracorporeal circulation over an immunoadsorbent in gel
Schenkein, Isaac, Bystryn, Jean-Claude, Uhr, Jonathan W.
A method is described for preparation of a solid immunoadsorbent suspended in an agar gel. The immunoadsorbent in gel retains its specificity. Rabbits were injected with antibody or antigen, and an...
Sherr, Charles J., Uhr, Jonathan W.
Mouse myeloma cells pulse-labeled in vitro with 3H-leucine or 3H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides...
Immunoglobulin Synthesis and Secretion by Cells in the Mouse Thymus That Do Not Bear θ Antigen
Vitetta, Ellen S., Uhr, Jonathan W., Boyse, Edward A.
The mouse thymus contains cells that synthesize and secrete minute amounts of immunoglobulin (Ig). These cells were studied by a combination of cytotoxic tests (with alloantisera prepared in congenic...
Marches, Radu, Hsueh, Robert, Uhr, Jonathan W.
The p21WAF1 (p21) cyclin-dependent kinase inhibitor plays a major role in regulating cell cycle arrest. It was recently reported that the p53-independent elevation of p21 protein levels is essential...
Detection and characterization of carcinoma cells in the blood
Racila, Emilian, Euhus, David, Weiss, Arthur J., Rao, Chandra, McConnell, John, ...
A highly sensitive assay combining immunomagnetic enrichment with multiparameter flow cytometric and immunocytochemical analysis has been developed to detect, enumerate, and characterize carcinoma...
Ghetie, Maria-Ana, Podar, Erika M., Ilgen, Amy, Gordon, Brian E., Uhr, Jonathan W., Vitetta, Ellen S.
Monoclonal antibodies (mAbs) that exert antitumor activity can do so by virtue of their effector function and/or their ability to signal growth arrest or cell death. In this study, we demonstrate...
A role for intracellular pH in membrane IgM-mediated cell death of human B lymphomas
Marches, Radu, Vitetta, Ellen S., Uhr, Jonathan W.
We show that anti-IgM-induced cell death in a human B lymphoma cell line, B104, is associated with early intracellular acidification and cell shrinkage. In contrast, another human B cell lymphoma...
Uhr, Jonathan W., Schenkein, Isaac
P3K cells were uniformly labeled with [14C]leucine and also for 1 hr with [3H]leucine, [3]galactose, or [3H]glucosamine. Subcellular fractionation of labeled cells was performed and the amounts of...
Sherr, Charles J., Uhr, Jonathan W.
Mouse myeloma cells were pulse-labeled in vitro with 3H-D-glucosamine and 14C-L-leucine. Analysis on sucrose gradients revealed incorporation of both isotopes into polyribosomes and release of most...
Specific removal of in vivo antibody by extracorporeal circulation over an immunoadsorbent in gel
Schenkein, Isaac, Bystryn, Jean-Claude, Uhr, Jonathan W.
A method is described for preparation of a solid immunoadsorbent suspended in an agar gel. The immunoadsorbent in gel retains its specificity. Rabbits were injected with antibody or antigen, and an...
Sherr, Charles J., Uhr, Jonathan W.
Mouse myeloma cells pulse-labeled in vitro with 3H-leucine or 3H-glucosamine were fractionated on sucrose gradients, and membrane-bound and free polyribosomes were separated. Nascent polypeptides...
Immunoglobulin Synthesis and Secretion by Cells in the Mouse Thymus That Do Not Bear θ Antigen
Vitetta, Ellen S., Uhr, Jonathan W., Boyse, Edward A.
The mouse thymus contains cells that synthesize and secrete minute amounts of immunoglobulin (Ig). These cells were studied by a combination of cytotoxic tests (with alloantisera prepared in congenic...
Uhr, Jonathan W., Salvin, S. B., Pappenheimer, A. M.
A general method for induction of the delayed hypersensitive state directed against single protein antigens is described. The method consists of intradermal injection of minute amounts of washed...
Uhr, Jonathan W., Pappenheimer, A. M., Yoneda, M.
Guinea pigs infected by intradermal injection of living toxigenic diphtheria bacilli and protected by horse antitoxic globulin, given either before or after infection, develop delayed...
DELAYED HYPERSENSITIVITY : IV. SYSTEMIC REACTIVITY OF GUINEA PIGS SENSITIZED TO PROTEIN ANTIGENS
Uhr, Jonathan W., Brandriss, M. W.
Guinea pigs with delayed hypersensitivity to protein antigens show a specific febrile response accompanied by a lymphopenia following injection of a desensitizing dose of specific antigen. No signs...
Uhr, Jonathan W., Pappenheimer, A. M.
Guinea pigs rendered hypersensitive (delayed-type) to protein antigen can be completely and specifically desensitized by a single injection containing a sufficient amount of the corresponding...
Uhr, Jonathan W., Scharff, Matthew
The capacity to develop the delayed type of hypersensitivity to diphtheria toxoid and ovalbumin may persist in guinea pigs and rabbits that have received doses of x-ray sufficient to eliminate a...
ANTIBODY FORMATION : II. THE SPECIFIC ANAMNESTIC ANTIBODY RESPONSE
Uhr, Jonathan W., Baumann, Joyce B.
Diphtheria toxoid-antitoxin precipitates formed in antitoxin excess can prepare guinea pigs, rats, and rabbits for a secondary type of antitoxin response. Priming may occur without the development of...
ANTIBODY FORMATION : I. THE SUPPRESSION OF ANTIBODY FORMATION BY PASSIVELY ADMINISTERED ANTIBODY
Uhr, Jonathan W., Baumann, Joyce B.
The suppression of antibody formation by passively administered antibody is influenced by the dose and nature of the antigen, type of immunization procedure, ratio of antibody to antigen, species...
Uhr, Jonathan W., Finkelstein, Martin S., Baumann, Joyce B.
Injection of a small bacteriophage φX 174 into guinea pigs results in an accelerated elimination of phage detectable as early as 24 hours after injection. The immune nature of the accelerated...
THE EFFECT OF BACTERIAL ENDOTOXIN ON THE NEWBORN GUINEA PIG
Newborn guinea pigs (less than 24 hours old) are highly susceptible to the lethal effect of endotoxin and susceptibility decreases with age. The vulnerability to endotoxin has not been attributed to...
FETAL RESPONSE TO ANTIGENIC STIMULUS : II. ANTIBODY PRODUCTION BY THE FETAL LAMB
Silverstein, Arthur M., Uhr, Jonathan W., Kraner, Keith L., Lukes, Robert J.
The fetal lamb in utero is able to form large amounts of specific antibody in response to antigenic stimulus as early as the 66th to 70th day of the 150 day gestation period. Among the several...
IMMUNOLOGIC TOLERANCE AFTER SPECIFIC IMMUNIZATION
Dorner, Marianne M., Uhr, Jonathan W.
Specific immunologic tolerance to bovine serum albumin (BSA) was induced in approximately one-half of the rabbits that had been primarily immunized and were prepared for a secondary antibody response...
THE CARRIAGE OF IMMUNOLOGICAL MEMORY BY SMALL LYMPHOCYTES IN THE RAT
Gowans, James L., Uhr, Jonathan W.
Lymphocytes were obtained from the thoracic duct of rats 1½ to 15 months after primary immunization with a single dose of bacteriophage φX 174. An intravenous injection of these lymphocytes...
MECHANISMS UNDERLYING BINDING OF IMMUNE COMPLEXES TO MACROPHAGES
Phillips-Quagliata, Julia M., Levine, Bernard B., Quagliata, Franco, Uhr, Jonathan W.
The mechanism of binding of immune complexes to macrophages was investigated using purified antibody and haptens of different valences. Antibody alone bound to macrophages; enhancement of binding...
Sherr, Charles J., Uhr, Jonathan W.
Cells from an established line of Burkitt's lymphoma (Daudi) and a mouse myeloma (P3K) were pulse-labeled in vitro with 3H-leucine, and immunoglobulin was immunologically precipitated from cell...
Vitetta, Ellen S., Baur, Sonia, Uhr, Jonathan W.
The proteins on surfaces of living splenic lymphocytes from normal BALB/c mice were iodinated enzymatically. Such cells were fractionated into two sub-populations: one composed almost exclusively of...
SIGNIFICANCE OF BIVALENCE OF ANTIBODY IN VIRAL NEUTRALIZATION
Rosenstein, Robert W., Nisonoff, Alfred, Uhr, Jonathan W.
The role of bivalence of antibody in its capacity to neutralize virus was studied with rabbit antibodies to the bacteriophage, φX174. Univalent Fab or Fab' fragments of IgG isolated from antiviral...
Sherr, Charles J., Baur, Sonia, Grundke, Inge, Zeligs, Joseph, Zeligs, Barbara, Uhr, Jonathan W.
Cells from an established line of Burkitt lymphoma (Daudi) were enzymatically radioiodinated, and labeled Ig from the cell surface was isolated and studied. Subcellular fractionation of labeled cells...
Vitetta, Ellen S., Bianco, Celso, Nussenzweig, Victor, Uhr, Jonathan W.
Thymocytes, bone marrow cells, and their derived T and B cell populations were examined for the presence of Ig by the cell surface radioiodination technique. Both IgM and IgG were identified on bone...
CELL SURFACE IMMUNOGLOBULIN : V. RELEASE FROM MURINE SPLENIC LYMPHOCYTES
Vitetta, Ellen S., Uhr, Jonathan W.
Turnover and release of cell surface Ig and secretion of total intracellular Ig has been studied in small lymphocytes from normal mouse spleen. The major findings to emerge are: (a) small lymphocytes...
AN ELECTROPHORETIC DIFFERENCE BETWEEN SURFACE AND SECRETED IGM OF MURINE SPLENOCYTES
Melcher, Ulrich, Uhr, Jonathan W.
µ-chains on the surface of murine splenocytes are more heterogeneous on SDS-polyacrylamide gel electrophoresis than both secreted and intracellular µ-chains labeled with [3H]tyrosine. No difference...
Bystryn, Jean-Claude, Siskind, Gregory W., Uhr, Jonathan W.
The binding of antigen to cells with antibody on their surface has been studied in a model system consisting of murine myeloma cells (MOPC 315) and DNP conjugates. Specific binding occurred between...
Vitetta, Ellen S., Grundke-Iqbal, Inge, Holmes, Kathryn V., Uhr, Jonathan W.
Lymphoid cells from the spleen, lymph nodes, and thoracic duct of axenic and control mice were incubated with [3H]tyrosine and synthesis and secretion of protein and Ig studied. It was found that...
Vitetta, Ellen S., Uhr, Jonathan W.
A new method for the detection of cell surface immunoglobulin labeled with isotopic precursors is described. The method consists of the aggregation of surface Ig on cells with specific antibody...
Melcher, Ulrich, Vitetta, Ellen S., McWilliams, Michael, Lamm, Michael E., Phillips-Quagliata, Julia M., Uhr, Jonathan W.
Uhr, Jonathan W., Finkelstein, Martin S.
Injection of a sufficient dose of bacteriophage φX 174 into guinea pigs results in the formation of rapidly sedimenting antibody molecules (19S), and later, slowly sedimenting molecules (7S). Above...
Graf, Martin W., Uhr, Jonathan W.
Rabbits were injected intravenously with bovine serum albumin (BSA) and bacteriophage T2 (T2). 2–3 wk later, anti-BSA was removed from such animals by a procedure which involved exposure of removed...
Bystryn, Jean-Claude, Graf, Martin W., Uhr, Jonathan W.
Rabbits were immunized to two antigens and 18–55 days later exchange transfusion was performed using blood of rabbits immunized to one antigen only. By this means, serum antibody levels to one...
Wernet, Dorothee, Vitetta, Ellen S., Uhr, Jonathan W., Boyse, Edward A.
A/J spleen cells were labeled with [3H]leucine and at intervals thereafter were homogenized and separated into microsomes and cell sap. Ig and H-2 antigens were assayed in the cell fractions and cell...
Zagury, Daniel, Uhr, Jonathan W., Jamieson, James D., Palade, George E.
The subcellular sites of synthesis and route of intracellular transfer of immunoglobulin G (IgG) have been investigated by electron microscope radioautography with precursors used for the polypeptide...
Schenkein, Isaac, Uhr, Jonathan W.
This study was designed to determine the time in the intracellular life of immunoglobulin when the carbohydrate moieties are added. Plasma cells from a mouse myeloma tumor were exposed to...