Length control of the metaphase spindle (2005)
Goshima, Gohta, Wollman, Roy, Stuurman, Nico, Scholey, Jonathan M., Vale, Ronald D.
Author Posting. © Elsevier B. V., 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version...
Length control of the metaphase spindle (2005)
Goshima, Gohta, Wollman, Roy, Stuurman, Nico, Scholey, Jonathan M., Vale, Ronald D.
Author Posting. © Elsevier B. V., 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version...
Snow, J J, Ou, G S, Gunnarson, A L, Walker, MRS, Zhou, H M, Brust-Mascher, I, ...
Cilia have diverse roles in motility and sensory reception and their dysfunction contributes to cilia-related diseases. Assembly and maintenance of cilia depends on the intraflagellar transport (IFT)...
Brust-Mascher, I, Civelekoglu-Scholey, G, Kwon, M, Mogilner, A, Scholey, Jonathan M
It has been proposed that the suppression of poleward flux within interpolar microtubule (ipMT) bundles of Drosophila embryonic spindles couples outward forces generated by a sliding filament...
Intraflagellar Transport (2003)
It has been a decade since a novel form of microtubule (MT)-based motility, i.e., intraflagellar transport (IFT), was discovered in Chlamydomonas flagella. Subsequent research has supported the...
Functional Coordination of Three Mitotic Motors in Drosophila EmbryosV⃞
Sharp, David J., Brown, Heather M., Kwon, Mijung, Rogers, Gregory C., Holland, Gina, Scholey, Jonathan M.
It is well established that multiple microtubule-based motors contribute to the formation and function of the mitotic spindle, but how the activities of these motors interrelate remains unclear. Here...
Signor, Dawn, Wedaman, Karen P., Rose, Lesilee S., Scholey, Jonathan M.
Chemosensation in the nervous system of the nematode Caenorhabditis elegans depends on sensory cilia, whose assembly and maintenance requires the transport of components such as axonemal proteins and...
Microtubule Flux and Sliding in Mitotic Spindles of Drosophila EmbryosV⃞
Brust-Mascher, Ingrid, Scholey, Jonathan M.
We proposed that spindle morphogenesis in Drosophila embryos involves progression through four transient isometric structures in which a constant spacing of the spindle poles is maintained by a...
Kwon, Mijung, Morales-Mulia, Sandra, Brust-Mascher, Ingrid, Rogers, Gregory C., Sharp, David J., Scholey, Jonathan M.
Mitosis requires the concerted activities of multiple microtubule (MT)-based motor proteins. Here we examined the contribution of the chromokinesin, KLP3A, to mitotic spindle morphogenesis and...
Morales-Mulia, Sandra, Scholey, Jonathan M.
Dynein is a critical mitotic motor whose inhibition causes defects in spindle pole organization and separation, chromosome congression or segregation, and anaphase spindle elongation, but results...
Functional Coordination of Three Mitotic Motors in Drosophila EmbryosV⃞
Sharp, David J., Brown, Heather M., Kwon, Mijung, Rogers, Gregory C., Holland, Gina, Scholey, Jonathan M.
It is well established that multiple microtubule-based motors contribute to the formation and function of the mitotic spindle, but how the activities of these motors interrelate remains unclear. Here...
Signor, Dawn, Wedaman, Karen P., Rose, Lesilee S., Scholey, Jonathan M.
Chemosensation in the nervous system of the nematode Caenorhabditis elegans depends on sensory cilia, whose assembly and maintenance requires the transport of components such as axonemal proteins and...
Microtubule Flux and Sliding in Mitotic Spindles of Drosophila EmbryosV⃞
Brust-Mascher, Ingrid, Scholey, Jonathan M.
We proposed that spindle morphogenesis in Drosophila embryos involves progression through four transient isometric structures in which a constant spacing of the spindle poles is maintained by a...
Kwon, Mijung, Morales-Mulia, Sandra, Brust-Mascher, Ingrid, Rogers, Gregory C., Sharp, David J., Scholey, Jonathan M.
Mitosis requires the concerted activities of multiple microtubule (MT)-based motor proteins. Here we examined the contribution of the chromokinesin, KLP3A, to mitotic spindle morphogenesis and...
Morales-Mulia, Sandra, Scholey, Jonathan M.
Dynein is a critical mitotic motor whose inhibition causes defects in spindle pole organization and separation, chromosome congression or segregation, and anaphase spindle elongation, but results...
Efimenko, Evgeni, Blacque, Oliver E., Ou, Guangshuo, Haycraft, Courtney J., Yoder, Bradley K., Scholey, Jonathan M., ...
The intraflagellar transport (IFT) machinery required to build functional cilia consists of a multisubunit complex whose molecular composition, organization, and function are poorly understood. Here,...
The WD Repeat-containing Protein IFTA-1 Is Required for Retrograde Intraflagellar Transport
Blacque, Oliver E., Li, Chunmei, Inglis, Peter N., Esmail, Muneer A., Ou, Guangshuo, Mah, Allan K., ...
The assembly and maintenance of cilia require intraflagellar transport (IFT), a microtubule-dependent bidirectional motility of multisubunit protein complexes along ciliary axonemes. Defects in IFT...
Ou, Guangshuo, Koga, Makato, Blacque, Oliver E., Murayama, Takashi, Ohshima, Yasumi, Schafer, Jenny C., ...
Sensory cilium biogenesis within Caenorhabditis elegans neurons depends on the kinesin-2–dependent intraflagellar transport (IFT) of ciliary precursors associated with IFT particles to the axoneme...
Evans, James E., Snow, Joshua J., Gunnarson, Amy L., Ou, Guangshuo, Stahlberg, Henning, McDonald, Kent L., ...
The diversity of sensory cilia on Caenorhabditis elegans neurons allows the animal to detect a variety of sensory stimuli. Sensory cilia are assembled by intraflagellar transport (IFT) kinesins,...
Pan, Xiaoyu, Ou, Guangshuo, Civelekoglu-Scholey, Gul, Blacque, Oliver E., Endres, Nicholas F., Tao, Li, ...
The assembly and function of cilia on Caenorhabditis elegans neurons depends on the action of two kinesin-2 motors, heterotrimeric kinesin-II and homodimeric OSM-3–kinesin, which cooperate to move...
Quantitative analysis of an anaphase B switch: predicted role for a microtubule catastrophe gradient
Cheerambathur, Dhanya K., Civelekoglu-Scholey, Gul, Brust-Mascher, Ingrid, Sommi, Patrizia, Mogilner, Alex, Scholey, Jonathan M.
Anaphase B in Drosophila embryos is initiated by the inhibition of microtubule (MT) depolymerization at spindle poles, which allows outwardly sliding interpolar (ip) MTs to drive pole–pole...
A standardized kinesin nomenclature
Lawrence, Carolyn J., Dawe, R. Kelly, Christie, Karen R., Cleveland, Don W., Dawson, Scott C., Endow, Sharyn A., ...
In recent years the kinesin superfamily has become so large that several different naming schemes have emerged, leading to confusion and miscommunication. Here, we set forth a standardized kinesin...
Kinesin- and Myosin-driven Steps of Vesicle Recruitment for Ca2+-regulated Exocytosis
Bi, Guo-Qiang, Morris, Robert L., Liao, Guochun, Alderton, Janet M., Scholey, Jonathan M., Steinhardt, Richard A.
Kinesin and myosin have been proposed to transport intracellular organelles and vesicles to the cell periphery in several cell systems. However, there has been little direct observation of the role...
Morris, Robert L., Scholey, Jonathan M.
Heterotrimeric kinesin-II is a plus end– directed microtubule (MT) motor protein consisting of distinct heterodimerized motor subunits associated with an accessory subunit. To probe the...
Sharp, David J., McDonald, Kent L., Brown, Heather M., Matthies, Heinrich J., Walczak, Claire, Vale, Ron D., ...
Previous genetic and biochemical studies have led to the hypothesis that the essential mitotic bipolar kinesin, KLP61F, cross-links and slides microtubules (MTs) during spindle assembly and function....
Mitosis, microtubules, and the matrix
Scholey, Jonathan M., Rogers, Gregory C., Sharp, David J.
The mechanical events of mitosis depend on the action of microtubules and mitotic motors, but whether these spindle components act alone or in concert with a spindle matrix is an important question.
Signor, Dawn, Wedaman, Karen P., Orozco, Jose T., Dwyer, Noelle D., Bargmann, Cornelia I., Rose, Lesilee S., ...
The heterotrimeric motor protein, kinesin-II, and its presumptive cargo, can be observed moving anterogradely at 0.7 μm/s by intraflagellar transport (IFT) within sensory cilia of chemosensory...
Rogers, Gregory C., Chui, Kitty K., Lee, Edwin W., Wedaman, Karen P., Sharp, David J., Holland, Gina, ...
We have investigated the intracellular roles of an Xklp2-related kinesin motor, KRP180, in positioning spindle poles during early sea urchin embryonic cell division using quantitative, real-time...
Intraflagellar transport motors in cilia: moving along the cell's antenna
Intraflagellar transport (IFT), the motor-dependent movement of IFT particles along the axoneme, is critical for the assembly, maintenance, and function of motile and sensory cilia, and,...
Reverse engineering of force integration during mitosis in the Drosophila embryo
Wollman, Roy, Civelekoglu-Scholey, Gul, Scholey, Jonathan M, Mogilner, Alex
The mitotic spindle is a complex macromolecular machine that coordinates accurate chromosome segregation. The spindle accomplishes its function using forces generated by microtubules (MTs) and...