John K. Rose

Inhibition of translation by poliovirus: Inactivation of a specific initiation factor (1978)

Rose, John K., Trachsel, Hans, Leong, Kahan, Baltimore, David

Translation of vesicular stomatitis virus (VSV) mRNA, like host mRNA translation, is inhibited in cells infected with poliovirus. To study the mechanism of poliovirus-induced inhibition of protein...

5'-Terminus of Moloney Murine Leukemia Virus 35S RNA Is m7G5' ppp5' GmpCp (1976)

Rose, John K., Haseltine, William A., Baltimore, David

The 5'-terminal sequence m7G5' ppp5' GmpCp was isolated from Moloney murine leukemia virus 35S RNA after digestion of 32P-labeled RNA with RNases T1, T2, and A followed by pH 3.5 ionophoresis on DEAE...

5'-terminal structure of poliovirus polyribosomal RNA is pUp (1976)

Hewlett, Martinez J., Rose, John K., Baltimore, David

Poliovirus RNA purified from virus-specific polyribosomes does not contain m7G in a 5'-5'-pyrophosphate linkage at its 5'-end. The only potential 5'-end found in ribonuclease digests of this RNA is...

Generation of mucosal cytotoxic T cells against soluble protein by tissue-specific environmental and costimulatory signals

Kim, Sung-Kwon, Reed, Douglas S., Olson, Sara, Schnell, Matthias J., Rose, John K., Morton, Phillip A., ...

We compared peripheral and mucosal primary CD8 T cell responses to inflammatory and noninflammatory forms of antigen in a T cell-adoptive transfer system. Immunization with the soluble antigen,...

Attenuated Vesicular Stomatitis Viruses as Vaccine Vectors

Roberts, Anjeanette, Buonocore, Linda, Price, Ryan, Forman, John, Rose, John K.

We showed previously that a single intranasal vaccination of mice with a recombinant vesicular stomatitis virus (VSV) expressing an influenza virus hemagglutinin (HA) protein provided complete...

Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing an Influenza Virus Hemagglutinin Provides Complete Protection from Influenza Virus Challenge

Roberts, Anjeanette, Kretzschmar, Evelyne, Perkins, Archibald S., Forman, John, Price, Ryan, Buonocore, Linda, ...

Since the development of a system for generating vesicular stomatitis virus (VSV) from plasmid DNAs, our laboratory has reported the expression of several different glycoproteins from recombinant...

Successful Vaccine-Induced Seroconversion by Single-Dose Immunization in the Presence of Measles Virus-Specific Maternal Antibodies

Schlereth, Bernd, Rose, John K., Buonocore, Linda, Ter Meulen, Volker, Niewiesk, Stefan

In humans, maternal antibodies inhibit successful immunization against measles, because they interfere with vaccine-induced seroconversion. We have investigated this problem using the cotton rat...

Replication-Competent Rhabdoviruses with Human Immunodeficiency Virus Type 1 Coats and Green Fluorescent Protein: Entry by a pH-Independent Pathway

Boritz, Eli, Gerlach, Jennifer, Johnson, J. Erik, Rose, John K.

We describe a replication-competent, recombinant vesicular stomatitis virus (VSV) in which the gene encoding the single transmembrane glycoprotein (G) was deleted and replaced by an env-G hybrid gene...

Glycoprotein Exchange Vectors Based on Vesicular Stomatitis Virus Allow Effective Boosting and Generation of Neutralizing Antibodies to a Primary Isolate of Human Immunodeficiency Virus Type 1

Rose, Nina F., Roberts, Anjeanette, Buonocore, Linda, Rose, John K.

Live recombinant vesicular stomatitis viruses (VSVs) expressing foreign antigens are highly effective vaccine vectors. However, these vectors induce high-titer neutralizing antibody directed at the...

Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge

Kahn, Jeffrey S., Roberts, Anjeanette, Weibel, Carla, Buonocore, Linda, Rose, John K.

Foreign glycoproteins expressed in recombinant vesicular stomatitis virus (VSV) can elicit specific and protective immunity in the mouse model. We have previously demonstrated the expression of...

Relative Neurotropism of a Recombinant Rhabdovirus Expressing a Green Fluorescent Envelope Glycoprotein

Van Den Pol, Anthony N., Dalton, Kevin P., Rose, John K.

A new recombinant vesicular stomatitis virus (rVSV) that expresses green fluorescent protein (GFP) on the cytoplasmic domain of the VSV glycoprotein (G protein) was used in the mouse as a model for...

High-Level Primary CD8+ T-Cell Response to Human Immunodeficiency Virus Type 1 Gag and Env Generated by Vaccination with Recombinant Vesicular Stomatitis Viruses

Haglund, Karl, Leiner, Ingrid, Kerksiek, Kristen, Buonocore, Linda, Pamer, Eric, Rose, John K.

We investigated the primary cellular immune responses to human immunodeficiency virus type 1 (HIV-1) Env and Gag proteins elicited by recombinant vesicular stomatitis viruses (rVSVs). The primary...

Characterization of Vesicular Stomatitis Virus Recombinants That Express and Incorporate High Levels of Hepatitis C Virus Glycoproteins

Buonocore, Linda, Blight, Keril J., Rice, Charles M., Rose, John K.

We generated recombinant vesicular stomatitis viruses (VSV) expressing genes encoding hybrid proteins consisting of the extracellular domains of hepatitis C virus (HCV) glycoproteins fused at...

Robust Recall and Long-Term Memory T-Cell Responses Induced by Prime-Boost Regimens with Heterologous Live Viral Vectors Expressing Human Immunodeficiency Virus Type 1 Gag and Env Proteins

Haglund, Karl, Leiner, Ingrid, Kerksiek, Kristen, Buonocore, Linda, Pamer, Eric, Rose, John K.

We investigated long-term memory and recall cellular immune responses to human immunodeficiency virus type 1 (HIV-1) Env and Gag proteins elicited by recombinant vesicular stomatitis viruses (VSVs)...

Intranasal Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing Cottontail Rabbit Papillomavirus L1 Protein Provides Complete Protection against Papillomavirus-Induced Disease

Reuter, Jon D., Vivas-Gonzalez, Beatriz E., Gomez, Daniel, Wilson, Jean H., Brandsma, Janet L., Greenstone, Heather L., ...

Immunizations with live recombinant vesicular stomatitis viruses (rVSV) expressing foreign viral proteins have successfully protected animals from challenges with several heterologous viruses. We...

Intranasal Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing Cottontail Rabbit Papillomavirus L1 Protein Provides Complete Protection against Papillomavirus-Induced Disease

Reuter, Jon D., Vivas-Gonzalez, Beatriz E., Gomez, Daniel, Wilson, Jean H., Brandsma, Janet L., Greenstone, Heather L., ...

Development of a Novel Surrogate Virus for Human T-Cell Leukemia Virus Type 1: Inhibition of Infection by Osteoprotegerin

Okuma, Kazu, Dalton, Kevin P., Buonocore, Linda, Ramsburg, Elizabeth, Rose, John K.

To develop a high-titer surrogate virus for human T-cell leukemia virus type 1 (HTLV-1), we generated recombinant vesicular stomatitis viruses (VSVs) in which the gene encoding the single...

Transcription of the Operator Proximal and Distal Ends of the Tryptophan Operon: Evidence that trpE and trpA Are the Delimiting Structural Genes

Rose, John K., Yanofsky, Charles

Measurements of the time required for transcription of regions of known genetic length in the tryptophan operon of Escherichia coli indicate that there are no major unidentified structural genes in...

Complete Protection from Papillomavirus Challenge after a Single Vaccination with a Vesicular Stomatitis Virus Vector Expressing High Levels of L1 Protein

Roberts, Anjeanette, Reuter, Jon D., Wilson, Jean H., Baldwin, Stuart, Rose, John K.

We generated an attenuated, recombinant vesicular stomatitis virus (VSV) expressing high levels of the cottontail rabbit papillomavirus (CRPV) L1 protein from an upstream site in the VSV genome....

5′-Terminus of Moloney Murine Leukemia Virus 35S RNA Is m7G5′ ppp5′ GmpCp

Rose, John K., Haseltine, William A., Baltimore, David

The 5′-terminal sequence m7G5′ ppp5′ GmpCp was isolated from Moloney murine leukemia virus 35S RNA after digestion of 32P-labeled RNA with RNases T1, T2, and A followed by pH 3.5 ionophoresis...

Highly Effective Control of an AIDS Virus Challenge in Macaques by Using Vesicular Stomatitis Virus and Modified Vaccinia Virus Ankara Vaccine Vectors in a Single-Boost Protocol

Ramsburg, Elizabeth, Rose, Nina F., Marx, Preston A., Mefford, Megan, Nixon, Douglas F., Moretto, Walter J., ...

Previous studies have shown that vaccination and boosting of rhesus macaques with attenuated vesicular stomatitis virus (VSV) vectors encoding Env and Gag proteins of simian immunodeficiency...

Interaction of the Operator of the Tryptophan Operon with Repressor

Rose, John K., Yanofsky, Charles

Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a...

Prediction and Identification of a Permissive Epitope Insertion Site in the Vesicular Stomatitis Virus Glycoprotein

Schlehuber, Lisa D., Rose, John K.

We developed a rational approach to identify a site in the vesicular stomatitis virus (VSV) glycoprotein (G) that is exposed on the protein surface and tolerant of foreign epitope insertion. The...

Characterization of Nonpathogenic, Live, Viral Vaccine Vectors Inducing Potent Cellular Immune Responses

Publicover, Jean, Ramsburg, Elizabeth, Rose, John K.

Experimental vaccines based on recombinant vesicular stomatitis viruses (VSV) expressing foreign viral proteins are protective in several animal disease models. Although these attenuated viruses are...

A Single-Cycle Vaccine Vector Based on Vesicular Stomatitis Virus Can Induce Immune Responses Comparable to Those Generated by a Replication-Competent Vector

Publicover, Jean, Ramsburg, Elizabeth, Rose, John K.

Live attenuated vaccine vectors based on recombinant vesicular stomatitis virus (VSV) are effective in several viral disease models. In this study, we asked if a VSV vector capable of only a single...

A Vesicular Stomatitis Virus Recombinant Expressing Granulocyte-Macrophage Colony-Stimulating Factor Induces Enhanced T-Cell Responses and Is Highly Attenuated for Replication in Animals

Ramsburg, Elizabeth, Publicover, Jean, Buonocore, Linda, Poholek, Amanda, Robek, Michael, Palin, Amy, ...

Live attenuated vectors based on recombinant vesicular stomatitis viruses (rVSVs) expressing foreign antigens are highly effective vaccines in animal models. In this study, we report that an rVSV...

Role of N-Linked Glycans in a Human Immunodeficiency Virus Envelope Glycoprotein: Effects on Protein Function and the Neutralizing Antibody Response

Quiñones-Kochs, Miriam I., Buonocore, Linda, Rose, John K.

The envelope (Env) glycoprotein of human immunodeficiency virus (HIV) contains 24 N-glycosylation sites covering much of the protein surface. It has been proposed that one role of these carbohydrates...

Rapid Pathogenesis Induced by a Vesicular Stomatitis Virus Matrix Protein Mutant: Viral Pathogenesis Is Linked to Induction of Tumor Necrosis Factor Alpha

Publicover, Jean, Ramsburg, Elizabeth, Robek, Michael, Rose, John K.

Vesicular stomatitis virus (VSV) matrix (M) protein blocks host mRNA export from the nucleus and thereby inhibits interferon induction in infected cells. M mutants with mutations of methionine 51...

Generation of mucosal cytotoxic T cells against soluble protein by tissue-specific environmental and costimulatory signals

Kim, Sung-Kwon, Reed, Douglas S., Olson, Sara, Schnell, Matthias J., Rose, John K., Morton, Phillip A., ...

We compared peripheral and mucosal primary CD8 T cell responses to inflammatory and noninflammatory forms of antigen in a T cell-adoptive transfer system. Immunization with the soluble antigen,...

Attenuated Vesicular Stomatitis Viruses as Vaccine Vectors

Roberts, Anjeanette, Buonocore, Linda, Price, Ryan, Forman, John, Rose, John K.

We showed previously that a single intranasal vaccination of mice with a recombinant vesicular stomatitis virus (VSV) expressing an influenza virus hemagglutinin (HA) protein provided complete...

Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing an Influenza Virus Hemagglutinin Provides Complete Protection from Influenza Virus Challenge

Roberts, Anjeanette, Kretzschmar, Evelyne, Perkins, Archibald S., Forman, John, Price, Ryan, Buonocore, Linda, ...

Since the development of a system for generating vesicular stomatitis virus (VSV) from plasmid DNAs, our laboratory has reported the expression of several different glycoproteins from recombinant...

Successful Vaccine-Induced Seroconversion by Single-Dose Immunization in the Presence of Measles Virus-Specific Maternal Antibodies

Schlereth, Bernd, Rose, John K., Buonocore, Linda, Ter Meulen, Volker, Niewiesk, Stefan

In humans, maternal antibodies inhibit successful immunization against measles, because they interfere with vaccine-induced seroconversion. We have investigated this problem using the cotton rat...

Replication-Competent Rhabdoviruses with Human Immunodeficiency Virus Type 1 Coats and Green Fluorescent Protein: Entry by a pH-Independent Pathway

Boritz, Eli, Gerlach, Jennifer, Johnson, J. Erik, Rose, John K.

We describe a replication-competent, recombinant vesicular stomatitis virus (VSV) in which the gene encoding the single transmembrane glycoprotein (G) was deleted and replaced by an env-G hybrid gene...

Glycoprotein Exchange Vectors Based on Vesicular Stomatitis Virus Allow Effective Boosting and Generation of Neutralizing Antibodies to a Primary Isolate of Human Immunodeficiency Virus Type 1

Rose, Nina F., Roberts, Anjeanette, Buonocore, Linda, Rose, John K.

Live recombinant vesicular stomatitis viruses (VSVs) expressing foreign antigens are highly effective vaccine vectors. However, these vectors induce high-titer neutralizing antibody directed at the...

Replication-Competent or Attenuated, Nonpropagating Vesicular Stomatitis Viruses Expressing Respiratory Syncytial Virus (RSV) Antigens Protect Mice against RSV Challenge

Kahn, Jeffrey S., Roberts, Anjeanette, Weibel, Carla, Buonocore, Linda, Rose, John K.

Foreign glycoproteins expressed in recombinant vesicular stomatitis virus (VSV) can elicit specific and protective immunity in the mouse model. We have previously demonstrated the expression of...

Relative Neurotropism of a Recombinant Rhabdovirus Expressing a Green Fluorescent Envelope Glycoprotein

Van Den Pol, Anthony N., Dalton, Kevin P., Rose, John K.

A new recombinant vesicular stomatitis virus (rVSV) that expresses green fluorescent protein (GFP) on the cytoplasmic domain of the VSV glycoprotein (G protein) was used in the mouse as a model for...

High-Level Primary CD8+ T-Cell Response to Human Immunodeficiency Virus Type 1 Gag and Env Generated by Vaccination with Recombinant Vesicular Stomatitis Viruses

Haglund, Karl, Leiner, Ingrid, Kerksiek, Kristen, Buonocore, Linda, Pamer, Eric, Rose, John K.

We investigated the primary cellular immune responses to human immunodeficiency virus type 1 (HIV-1) Env and Gag proteins elicited by recombinant vesicular stomatitis viruses (rVSVs). The primary...

Characterization of Vesicular Stomatitis Virus Recombinants That Express and Incorporate High Levels of Hepatitis C Virus Glycoproteins

Buonocore, Linda, Blight, Keril J., Rice, Charles M., Rose, John K.

We generated recombinant vesicular stomatitis viruses (VSV) expressing genes encoding hybrid proteins consisting of the extracellular domains of hepatitis C virus (HCV) glycoproteins fused at...

Robust Recall and Long-Term Memory T-Cell Responses Induced by Prime-Boost Regimens with Heterologous Live Viral Vectors Expressing Human Immunodeficiency Virus Type 1 Gag and Env Proteins

Haglund, Karl, Leiner, Ingrid, Kerksiek, Kristen, Buonocore, Linda, Pamer, Eric, Rose, John K.

We investigated long-term memory and recall cellular immune responses to human immunodeficiency virus type 1 (HIV-1) Env and Gag proteins elicited by recombinant vesicular stomatitis viruses (VSVs)...

Intranasal Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing Cottontail Rabbit Papillomavirus L1 Protein Provides Complete Protection against Papillomavirus-Induced Disease

Reuter, Jon D., Vivas-Gonzalez, Beatriz E., Gomez, Daniel, Wilson, Jean H., Brandsma, Janet L., Greenstone, Heather L., ...

Immunizations with live recombinant vesicular stomatitis viruses (rVSV) expressing foreign viral proteins have successfully protected animals from challenges with several heterologous viruses. We...

Intranasal Vaccination with a Recombinant Vesicular Stomatitis Virus Expressing Cottontail Rabbit Papillomavirus L1 Protein Provides Complete Protection against Papillomavirus-Induced Disease

Reuter, Jon D., Vivas-Gonzalez, Beatriz E., Gomez, Daniel, Wilson, Jean H., Brandsma, Janet L., Greenstone, Heather L., ...

Role of N-Linked Glycans in a Human Immunodeficiency Virus Envelope Glycoprotein: Effects on Protein Function and the Neutralizing Antibody Response

Quiñones-Kochs, Miriam I., Buonocore, Linda, Rose, John K.

The envelope (Env) glycoprotein of human immunodeficiency virus (HIV) contains 24 N-glycosylation sites covering much of the protein surface. It has been proposed that one role of these carbohydrates...

Development of a Novel Surrogate Virus for Human T-Cell Leukemia Virus Type 1: Inhibition of Infection by Osteoprotegerin

Okuma, Kazu, Dalton, Kevin P., Buonocore, Linda, Ramsburg, Elizabeth, Rose, John K.

To develop a high-titer surrogate virus for human T-cell leukemia virus type 1 (HTLV-1), we generated recombinant vesicular stomatitis viruses (VSVs) in which the gene encoding the single...

Transcription of the Operator Proximal and Distal Ends of the Tryptophan Operon: Evidence that trpE and trpA Are the Delimiting Structural Genes

Rose, John K., Yanofsky, Charles

Measurements of the time required for transcription of regions of known genetic length in the tryptophan operon of Escherichia coli indicate that there are no major unidentified structural genes in...

Complete Protection from Papillomavirus Challenge after a Single Vaccination with a Vesicular Stomatitis Virus Vector Expressing High Levels of L1 Protein

Roberts, Anjeanette, Reuter, Jon D., Wilson, Jean H., Baldwin, Stuart, Rose, John K.

We generated an attenuated, recombinant vesicular stomatitis virus (VSV) expressing high levels of the cottontail rabbit papillomavirus (CRPV) L1 protein from an upstream site in the VSV genome....

5′-Terminus of Moloney Murine Leukemia Virus 35S RNA Is m7G5′ ppp5′ GmpCp

Rose, John K., Haseltine, William A., Baltimore, David

The 5′-terminal sequence m7G5′ ppp5′ GmpCp was isolated from Moloney murine leukemia virus 35S RNA after digestion of 32P-labeled RNA with RNases T1, T2, and A followed by pH 3.5 ionophoresis...

Highly Effective Control of an AIDS Virus Challenge in Macaques by Using Vesicular Stomatitis Virus and Modified Vaccinia Virus Ankara Vaccine Vectors in a Single-Boost Protocol

Ramsburg, Elizabeth, Rose, Nina F., Marx, Preston A., Mefford, Megan, Nixon, Douglas F., Moretto, Walter J., ...

Previous studies have shown that vaccination and boosting of rhesus macaques with attenuated vesicular stomatitis virus (VSV) vectors encoding Env and Gag proteins of simian immunodeficiency...

Interaction of the Operator of the Tryptophan Operon with Repressor

Rose, John K., Yanofsky, Charles

Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a...

Prediction and Identification of a Permissive Epitope Insertion Site in the Vesicular Stomatitis Virus Glycoprotein

Schlehuber, Lisa D., Rose, John K.

We developed a rational approach to identify a site in the vesicular stomatitis virus (VSV) glycoprotein (G) that is exposed on the protein surface and tolerant of foreign epitope insertion. The...

Characterization of Nonpathogenic, Live, Viral Vaccine Vectors Inducing Potent Cellular Immune Responses

Publicover, Jean, Ramsburg, Elizabeth, Rose, John K.

Experimental vaccines based on recombinant vesicular stomatitis viruses (VSV) expressing foreign viral proteins are protective in several animal disease models. Although these attenuated viruses are...

A Single-Cycle Vaccine Vector Based on Vesicular Stomatitis Virus Can Induce Immune Responses Comparable to Those Generated by a Replication-Competent Vector

Publicover, Jean, Ramsburg, Elizabeth, Rose, John K.

Live attenuated vaccine vectors based on recombinant vesicular stomatitis virus (VSV) are effective in several viral disease models. In this study, we asked if a VSV vector capable of only a single...

A Vesicular Stomatitis Virus Recombinant Expressing Granulocyte-Macrophage Colony-Stimulating Factor Induces Enhanced T-Cell Responses and Is Highly Attenuated for Replication in Animals

Ramsburg, Elizabeth, Publicover, Jean, Buonocore, Linda, Poholek, Amanda, Robek, Michael, Palin, Amy, ...

Live attenuated vectors based on recombinant vesicular stomatitis viruses (rVSVs) expressing foreign antigens are highly effective vaccines in animal models. In this study, we report that an rVSV...

Rapid Pathogenesis Induced by a Vesicular Stomatitis Virus Matrix Protein Mutant: Viral Pathogenesis Is Linked to Induction of Tumor Necrosis Factor Alpha

Publicover, Jean, Ramsburg, Elizabeth, Robek, Michael, Rose, John K.

Vesicular stomatitis virus (VSV) matrix (M) protein blocks host mRNA export from the nucleus and thereby inhibits interferon induction in infected cells. M mutants with mutations of methionine 51...

Replication and Propagation of Attenuated Vesicular Stomatitis Virus Vectors In Vivo: Vector Spread Correlates with Induction of Immune Responses and Persistence of Genomic RNA▿

Simon, Ian D., Publicover, Jean, Rose, John K.

Live-attenuated vesicular stomatitis virus (VSV) vectors expressing foreign antigens induce potent immune responses and protect against viral diseases in animal models. Highly attenuated (VSV-CT1) or...

Vesicular Stomatitis Virus-Based Therapeutic Vaccination Targeted to the E1, E2, E6, and E7 Proteins of Cottontail Rabbit Papillomavirus▿

Brandsma, Janet L., Shylankevich, Mark, Su, Yuhua, Roberts, Anjeanette, Rose, John K., Zelterman, Daniel, ...

Persistent human papillomavirus (HPV)-associated benign and malignant lesions are a major cause of morbidity and mortality worldwide. Vaccination against HPV early proteins could provide an effective...

Single-Dose, Therapeutic Vaccination of Mice with Vesicular Stomatitis Virus Expressing Human Papillomavirus Type 16 E7 Protein▿

Liao, John B., Publicover, Jean, Rose, John K., DiMaio, Daniel

We are developing recombinant attenuated vesicular stomatitis virus (VSV) as a vaccine vector to generate humoral and cell-mediated immune responses. Here, we explore the use of VSV vaccines for...

Hybrid alphavirus–rhabdovirus propagating replicon particles are versatile and potent vaccine vectors

Rose, Nina F., Publicover, Jean, Chattopadhyay, Anasuya, Rose, John K.

Self-propagating, infectious, virus-like particles are generated in animal cell lines transfected with a Semliki Forest virus RNA replicon encoding a single viral structural protein, the vesicular...