Nir Friedman, Shuki Vardi, Michal Ronen, Uri Alon, Joel Stavans
Oscillations in transcriptional activity in the network responsible for controlling DNA damage are monitored with GFP- promoter fusions in individual E. coli cells.
Nir Friedman, Shuki Vardi, Michal Ronen, Uri Alon, Joel Stavans
The SOS genetic network is responsible for the repair/bypass of DNA damage in bacterial cells. While the initial stages of the response have been well characterized, less is known about the dynamics...
Objective-Type Dark-Field Illumination for Scattering from Microbeads (2001)
Braslavsky, Ido, Amit, Roee, Ali, B. M. Jaffar, Gileadi, Opher, Oppenheim, Amos, Stavans, Joel
We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the back-reflected illumination beam in an objective-type total internal...
Coiling Instability of Multilamellar Membrane Tubes with Anchored Polymers (2000)
Tsafrir, Ilan, Guedeau-Boudeville, Marie-Alice, Kandel, Daniel, Stavans, Joel
We study experimentally a coiling instability of cylindrical multilamellar stacks of phospholipid membranes, induced by polymers with hydrophobic anchors grafted along their hydrophilic backbone. Our...
Pearling Instabilities of Membrane Tubes with Anchored Polymers (2000)
Tsafrir, Ilan, Sagi, Dror, Arzi, Tamar, Guedeau-Boudeville, Marie-Alice, Frette, Vidar, Kandel, Daniel, ...
We have studied the pearling instability induced on hollow tubular lipid vesicles by hydrophilic polymers with hydrophobic side groups along the backbone. The results show that the polymer...
Coiling of Cylindrical Membrane Stacks with Anchored Polymers (1999)
Frette, Vidar, Tsafrir, Ilan, Guedeau-Boudeville, Marie-Alice, Jullien, Ludovic, Kandel, Daniel, Stavans, Joel
We study experimentally a coiling instability of cylindrical multilamellar stacks of phospholipid membranes, induced by polymers with hydrophobic anchors grafted along their hydrophilic backbone. We...
Compaction of single DNA molecules induced by binding of integration host factor (IHF)
Ali, B. M. Jaffar, Amit, Roee, Braslavsky, Ido, Oppenheim, Amos B., Gileadi, Opher, Stavans, Joel
We studied the interaction between the integration host factor (IHF), a major nucleoid-associated protein in bacteria, and single DNA molecules. Force–extension measurements of λ DNA and an...
Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions
Amit, Roee, Gileadi, Opher, Stavans, Joel
Holliday junctions form during DNA repair and homologous recombination processes. These processes entail branch migration, whereby the length of two arms of a cruciform increases at the expense of...
Increased Bending Rigidity of Single DNA Molecules by H-NS, a Temperature and Osmolarity Sensor
Amit, Roee, Oppenheim, Amos B., Stavans, Joel
Histonelike nucleoid structuring protein (H-NS) is an abundant prokaryotic protein participating in nucleoid structure, gene regulation, and silencing. It plays a key role in cell response to changes...
Precise Temporal Modulation in the Response of the SOS DNA Repair Network in Individual Bacteria
Friedman, Nir, Vardi, Shuki, Ronen, Michal, Alon, Uri, Stavans, Joel
The SOS genetic network is responsible for the repair/bypass of DNA damage in bacterial cells. While the initial stages of the response have been well characterized, less is known about the dynamics...
High fidelity of RecA-catalyzed recombination: a watchdog of genetic diversity
Sagi, Dror, Tlusty, Tsvi, Stavans, Joel
Homologous recombination plays a key role in generating genetic diversity, while maintaining protein functionality. The mechanisms by which RecA enables a single-stranded segment of DNA to recognize...
Compaction of single DNA molecules induced by binding of integration host factor (IHF)
Ali, B. M. Jaffar, Amit, Roee, Braslavsky, Ido, Oppenheim, Amos B., Gileadi, Opher, Stavans, Joel
We studied the interaction between the integration host factor (IHF), a major nucleoid-associated protein in bacteria, and single DNA molecules. Force–extension measurements of λ DNA and an...
Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions
Amit, Roee, Gileadi, Opher, Stavans, Joel
Holliday junctions form during DNA repair and homologous recombination processes. These processes entail branch migration, whereby the length of two arms of a cruciform increases at the expense of...
Quantitative kinetic analysis of the bacteriophage λ genetic network
Kobiler, Oren, Rokney, Assaf, Friedman, Nir, Court, Donald L., Stavans, Joel, Oppenheim, Amos B.
The lysis–lysogeny decision of bacteriophage λ has been a paradigm for a developmental genetic network, which is composed of interlocked positive and negative feedback loops. This genetic network...
Precise Temporal Modulation in the Response of the SOS DNA Repair Network in Individual Bacteria
Friedman, Nir, Vardi, Shuki, Ronen, Michal, Alon, Uri, Stavans, Joel
The SOS genetic network is responsible for the repair/bypass of DNA damage in bacterial cells. While the initial stages of the response have been well characterized, less is known about the dynamics...
Increased Bending Rigidity of Single DNA Molecules by H-NS, a Temperature and Osmolarity Sensor
Amit, Roee, Oppenheim, Amos B., Stavans, Joel
Histonelike nucleoid structuring protein (H-NS) is an abundant prokaryotic protein participating in nucleoid structure, gene regulation, and silencing. It plays a key role in cell response to changes...
High fidelity of RecA-catalyzed recombination: a watchdog of genetic diversity
Sagi, Dror, Tlusty, Tsvi, Stavans, Joel
Homologous recombination plays a key role in generating genetic diversity, while maintaining protein functionality. The mechanisms by which RecA enables a single-stranded segment of DNA to recognize...
Noise in timing and precision of gene activities in a genetic cascade
Amir, Amnon, Kobiler, Oren, Rokney, Assaf, Oppenheim, Amos B, Stavans, Joel
Biological developmental pathways require proper timing of gene expression. We investigated timing variations of defined steps along the lytic cascade of bacteriophage λ. Gene expression was...
Host responses influence on the induction of lambda prophage
Rokney, Assaf, Kobiler, Oren, Amir, Amnon, Court, Donald L, Stavans, Joel, Adhya, Sankar, ...
Inactivation of bacteriophage lambda CI repressor leads almost exclusively to lytic development. Prophage induction can be initiated either by DNA damage or by heat treatment of a...