Production of highly knotted DNA by means of cosmid circularization inside phage capsids (2007)
Trigueros, Sonia, Roca, Joaquim
Abstract Background The formation of DNA knots is common during biological transactions. Yet, functional implications of knotted DNA are not fully understood. Moreover, potential applications of DNA...
Production of highly knotted DNA by means of cosmid circularization inside phage capsids (2007)
Trigueros, Sonia, Roca, Joaquim
This article is available from: http://www.biomedcentral.com/1472-6750/7/94
Fractal Dimension and Localization of DNA Knots (2006)
Ercolini, Erika, Valle, Francesco, Adamcik, Jozef, Witz, Guillaume, Metzler, Ralf, Rios, Paolo De Los, ...
The scaling properties of DNA knots of different complexities were studied by atomic force microscope. Following two different protocols DNA knots are adsorbed onto a mica surface in regimes of (i)...
Novel display of knotted DNA molecules by two-dimensional gel electrophoresis
Trigueros, Sonia, Arsuaga, Javier, Vazquez, Maria E., Sumners, De Witt, Roca, Joaquim
We describe a two-dimensional agarose gel electrophoresis procedure that improves the resolution of knotted DNA molecules. The first gel dimension is run at low voltage, and DNA knots migrate...
Knotting probability of DNA molecules confined in restricted volumes: DNA knotting in phage capsids
Arsuaga, Javier, Vázquez, Mariel, Trigueros, Sonia, Sumners, De Witt, Roca, Joaquim
When linear double-stranded DNA is packed inside bacteriophage capsids, it becomes highly compacted. However, the phage is believed to be fully effective only if the DNA is not entangled....
Hanke, Tobias, Ramiro, María J., Trigueros, Sonia, Roca, Joaquim, Larraga, Vicente
We identified a type II topoisomerase enzyme from Leishmania infantum, a parasite protozoon causing disease in humans. This protein, named Li topo II, which displays a variable C-terminal end, is...
DNA knots reveal a chiral organization of DNA in phage capsids
Arsuaga, Javier, Vazquez, Mariel, McGuirk, Paul, Trigueros, Sonia, Sumners, De Witt, Roca, Joaquim
Icosahedral bacteriophages pack their double-stranded DNA genomes to near-crystalline density and achieve one of the highest levels of DNA condensation found in nature. Despite numerous studies, some...
Novel display of knotted DNA molecules by two-dimensional gel electrophoresis
Trigueros, Sonia, Arsuaga, Javier, Vazquez, Maria E., Sumners, De Witt, Roca, Joaquim
We describe a two-dimensional agarose gel electrophoresis procedure that improves the resolution of knotted DNA molecules. The first gel dimension is run at low voltage, and DNA knots migrate...
Knotting probability of DNA molecules confined in restricted volumes: DNA knotting in phage capsids
Arsuaga, Javier, Vázquez, Mariel, Trigueros, Sonia, Sumners, De Witt, Roca, Joaquim
When linear double-stranded DNA is packed inside bacteriophage capsids, it becomes highly compacted. However, the phage is believed to be fully effective only if the DNA is not entangled....
Hanke, Tobias, Ramiro, María J., Trigueros, Sonia, Roca, Joaquim, Larraga, Vicente
We identified a type II topoisomerase enzyme from Leishmania infantum, a parasite protozoon causing disease in humans. This protein, named Li topo II, which displays a variable C-terminal end, is...
DNA knots reveal a chiral organization of DNA in phage capsids
Arsuaga, Javier, Vazquez, Mariel, McGuirk, Paul, Trigueros, Sonia, Sumners, De Witt, Roca, Joaquim
Icosahedral bacteriophages pack their double-stranded DNA genomes to near-crystalline density and achieve one of the highest levels of DNA condensation found in nature. Despite numerous studies, some...
Topoisomerase II, not topoisomerase I, is the proficient relaxase of nucleosomal DNA
Salceda, Javier, Fernández, Xavier, Roca, Joaquim
Eukaryotic topoisomerases I and II efficiently remove helical tension in naked DNA molecules. However, this activity has not been examined in nucleosomal DNA, their natural substrate. Here, we...