Functional and structural studies of Escherichia coli RNase P RNA /--by Joanna Kufel. (1996)
Thesis (doctoral)--Uppsala University, 1996.
Genetic and Physical Interactions Involving the Yeast Nuclear Cap-Binding Complex
Fortes, Puri, Kufel, Joanna, Fornerod, Maarten, Polycarpou-Schwarz, Maria, Lafontaine, Denis, Tollervey, David, ...
Yeast strains lacking the yeast nuclear cap-binding complex (yCBC) are viable, although impaired in growth. We have taken advantage of this observation to carry out a genetic screen for components...
Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David
Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...
Kufel, Joanna, Allmang, Christine, Verdone, Loredana, Beggs, Jean D., Tollervey, David
Depletion of any of the five essential proteins Lsm2p to Lsm5p and Lsm8p leads to strong accumulation of all tested unspliced pre-tRNA species, as well as accumulation of 5′ and 3′ unprocessed...
A complex pathway for 3′ processing of the yeast U3 snoRNA
Kufel, Joanna, Allmang, Christine, Verdone, Loredana, Beggs, Jean, Tollervey, David
Mature U3 snoRNA in yeast is generated from the 3′-extended precursors by endonucleolytic cleavage followed by exonucleolytic trimming. These precursors terminate in poly(U) tracts and are normally...
Nuclear Pre-mRNA Decapping and 5′ Degradation in Yeast Require the Lsm2-8p Complex
Kufel, Joanna, Bousquet-Antonelli, Cecile, Beggs, Jean D., Tollervey, David
Previous analyses have identified related cytoplasmic Lsm1-7p and nuclear Lsm2-8p complexes. Here we report that mature heat shock and MET mRNAs that are trapped in the nucleus due to a block in mRNA...
Kikovska, Ema, Brännvall, Mathias, Kufel, Joanna, Kirsebom, Leif A.
Like the translational elongation factor EF-Tu, RNase P interacts with a large number of substrates where RNase P with its RNA subunit generates tRNAs with matured 5′ termini by cleaving tRNA...
3′-processing of yeast tRNATrp precedes 5′-processing
KUFEL, JOANNA, TOLLERVEY, DAVID
Previous analyses of eukaryotic pre-tRNAs processing have reported that 5′-cleavage by RNase P precedes 3′-maturation. Here we report that in contrast to all other yeast tRNAs analyzed to date,...
Genetic and Physical Interactions Involving the Yeast Nuclear Cap-Binding Complex
Fortes, Puri, Kufel, Joanna, Fornerod, Maarten, Polycarpou-Schwarz, Maria, Lafontaine, Denis, Tollervey, David, ...
Yeast strains lacking the yeast nuclear cap-binding complex (yCBC) are viable, although impaired in growth. We have taken advantage of this observation to carry out a genetic screen for components...
Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David
Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...
Kufel, Joanna, Allmang, Christine, Verdone, Loredana, Beggs, Jean D., Tollervey, David
Depletion of any of the five essential proteins Lsm2p to Lsm5p and Lsm8p leads to strong accumulation of all tested unspliced pre-tRNA species, as well as accumulation of 5′ and 3′ unprocessed...
A complex pathway for 3′ processing of the yeast U3 snoRNA
Kufel, Joanna, Allmang, Christine, Verdone, Loredana, Beggs, Jean, Tollervey, David
Mature U3 snoRNA in yeast is generated from the 3′-extended precursors by endonucleolytic cleavage followed by exonucleolytic trimming. These precursors terminate in poly(U) tracts and are normally...
Nuclear Pre-mRNA Decapping and 5′ Degradation in Yeast Require the Lsm2-8p Complex
Kufel, Joanna, Bousquet-Antonelli, Cecile, Beggs, Jean D., Tollervey, David
Previous analyses have identified related cytoplasmic Lsm1-7p and nuclear Lsm2-8p complexes. Here we report that mature heat shock and MET mRNAs that are trapped in the nucleus due to a block in mRNA...
Kikovska, Ema, Brännvall, Mathias, Kufel, Joanna, Kirsebom, Leif A.
Like the translational elongation factor EF-Tu, RNase P interacts with a large number of substrates where RNase P with its RNA subunit generates tRNAs with matured 5′ termini by cleaving tRNA...
3′-processing of yeast tRNATrp precedes 5′-processing
KUFEL, JOANNA, TOLLERVEY, DAVID
Previous analyses of eukaryotic pre-tRNAs processing have reported that 5′-cleavage by RNase P precedes 3′-maturation. Here we report that in contrast to all other yeast tRNAs analyzed to date,...
Apoptotic signals induce specific degradation of ribosomal RNA in yeast
Mroczek, Seweryn, Kufel, Joanna
Organisms exposed to reactive oxygen species, generated endogenously during respiration or by environmental conditions, undergo oxidative stress. Stress response can either repair the damage or...