Joachim Hauber

HIV-1 Proviral DNA Excision Using an Evolved Recombinase (2007)

Sarkar, Indrani, Hauber, Ilona, Hauber, Joachim, Buchholz, Frank

HIV-1 integrates into the host chromosome and persists as a provirus flanked by long terminal repeats (LTRs). To date, treatment regimens primarily target the virus enzymes or virus-cell fusion, but...

The strength of the HIV-1 3' splice sites affects Rev function (2006)

Kammler, Susanne, Otte, Marianne, Hauber, Ilona, Kjems, Jørgen, Hauber, Joachim, Schaal, Heiner

Abstract Background The HIV-1 Rev protein is a key component in the early to late switch in HIV-1 splicing from early intronless (e.g. tat , rev ) to late intron-containing Rev-dependent (e.g. gag ,...

Interaction of the HIV-1 Rev cofactor eukaryotic initiation factor 5A with ribosomal protein L5

Schatz, Octavian, Oft, Martin, Dascher, Christiane, Schebesta, Michael, Rosorius, Olaf, Jaksche, Herbert, ...

It has previously been shown that interaction of eukaryotic initiation factor 5A (eIF-5A) with the Rev trans-activator protein of HIV-1 mediates the transport of unspliced or incompletely spliced...

Evidence for specific nucleocytoplasmic transport pathways used by leucine-rich nuclear export signals

Elfgang, Claudia, Rosorius, Olaf, Hofer, Lotte, Jaksche, Herbert, Hauber, Joachim, Bevec, Dorian

Various proteins with different biological activities have been observed to be translocated from the nucleus to the cytoplasm in an energy- and signal-dependent manner in eukaryotic cells. This...

Functional Analysis of the Human Immunodeficiency Virus Type 1 Rev Protein Oligomerization Interface

Thomas, Sarah L., Oft, Martin, Jaksche, Herbert, Casari, Georg, Heger, Peter, Dobrovnik, Marika, ...

The expression of human immunodeficiency virus type 1 (HIV-1) structural proteins requires the action of the viral trans-regulatory protein Rev. Rev is a nuclear shuttle protein that directly binds...

Multimer Formation Is Not Essential for Nuclear Export of Human T-Cell Leukemia Virus Type 1 Rex trans-Activator Protein

Heger, Peter, Rosorius, Olaf, Koch, Claudia, Casari, Georg, Grassmann, Ralph, Hauber, Joachim

The Rex trans-regulatory protein of human T-cell leukemia virus type 1 (HTLV-1) is required for the nuclear export of incompletely spliced and unspliced viral mRNAs and is therefore essential for...

Mature Dendritic Cells Infected with Herpes Simplex Virus Type 1 Exhibit Inhibited T-Cell Stimulatory Capacity

Kruse, Monika, Rosorius, Olaf, Krätzer, Friedrich, Stelz, Gerhard, Kuhnt, Christine, Schuler, Gerold, ...

Mature dendritic cells (DC) are the most potent antigen-presenting cells within the entire immune system. Interference with the function of these cells therefore constitutes a very powerful mechanism...

The Adenovirus Type 5 E1B-55K Oncoprotein Actively Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is Mediated by a CRM1-Independent Mechanism

Dosch, Tanja, Horn, Florian, Schneider, Grit, Krätzer, Friedrich, Dobner, Thomas, Hauber, Joachim, ...

The E1B-55K and E4orf6 proteins of adenovirus type 5 are involved in viral mRNA export. Here we demonstrate that adenovirus infection does not inhibit the function of the E1B-55K nuclear export...

Identification of cellular deoxyhypusine synthase as a novel target for antiretroviral therapy

Hauber, Ilona, Bevec, Dorian, Heukeshoven, Jochen, Krätzer, Friedrich, Horn, Florian, Choidas, Axel, ...

The introduction of highly active antiretroviral therapy (HAART) has significantly decreased morbidity and mortality among patients infected with HIV-1. However, HIV-1 can acquire resistance against...

Interaction of the HIV-1 Rev cofactor eukaryotic initiation factor 5A with ribosomal protein L5

Schatz, Octavian, Oft, Martin, Dascher, Christiane, Schebesta, Michael, Rosorius, Olaf, Jaksche, Herbert, ...

It has previously been shown that interaction of eukaryotic initiation factor 5A (eIF-5A) with the Rev trans-activator protein of HIV-1 mediates the transport of unspliced or incompletely spliced...

Evidence for specific nucleocytoplasmic transport pathways used by leucine-rich nuclear export signals

Elfgang, Claudia, Rosorius, Olaf, Hofer, Lotte, Jaksche, Herbert, Hauber, Joachim, Bevec, Dorian

Various proteins with different biological activities have been observed to be translocated from the nucleus to the cytoplasm in an energy- and signal-dependent manner in eukaryotic cells. This...

Functional Analysis of the Human Immunodeficiency Virus Type 1 Rev Protein Oligomerization Interface

Thomas, Sarah L., Oft, Martin, Jaksche, Herbert, Casari, Georg, Heger, Peter, Dobrovnik, Marika, ...

The expression of human immunodeficiency virus type 1 (HIV-1) structural proteins requires the action of the viral trans-regulatory protein Rev. Rev is a nuclear shuttle protein that directly binds...

Multimer Formation Is Not Essential for Nuclear Export of Human T-Cell Leukemia Virus Type 1 Rex trans-Activator Protein

Heger, Peter, Rosorius, Olaf, Koch, Claudia, Casari, Georg, Grassmann, Ralph, Hauber, Joachim

The Rex trans-regulatory protein of human T-cell leukemia virus type 1 (HTLV-1) is required for the nuclear export of incompletely spliced and unspliced viral mRNAs and is therefore essential for...

Mature Dendritic Cells Infected with Herpes Simplex Virus Type 1 Exhibit Inhibited T-Cell Stimulatory Capacity

Kruse, Monika, Rosorius, Olaf, Krätzer, Friedrich, Stelz, Gerhard, Kuhnt, Christine, Schuler, Gerold, ...

Mature dendritic cells (DC) are the most potent antigen-presenting cells within the entire immune system. Interference with the function of these cells therefore constitutes a very powerful mechanism...

The Adenovirus Type 5 E1B-55K Oncoprotein Actively Shuttles in Virus-Infected Cells, Whereas Transport of E4orf6 Is Mediated by a CRM1-Independent Mechanism

Dosch, Tanja, Horn, Florian, Schneider, Grit, Krätzer, Friedrich, Dobner, Thomas, Hauber, Joachim, ...

The E1B-55K and E4orf6 proteins of adenovirus type 5 are involved in viral mRNA export. Here we demonstrate that adenovirus infection does not inhibit the function of the E1B-55K nuclear export...

Identification of cellular deoxyhypusine synthase as a novel target for antiretroviral therapy

Hauber, Ilona, Bevec, Dorian, Heukeshoven, Jochen, Krätzer, Friedrich, Horn, Florian, Choidas, Axel, ...

The introduction of highly active antiretroviral therapy (HAART) has significantly decreased morbidity and mortality among patients infected with HIV-1. However, HIV-1 can acquire resistance against...

Intranuclear targeting and nuclear export of the adenovirus E1B-55K protein are regulated by SUMO1 conjugation

Kindsmüller, Kathrin, Groitl, Peter, Härtl, Barbara, Blanchette, Paola, Hauber, Joachim, Dobner, Thomas

We have investigated the requirements for CRM1-mediated nuclear export and SUMO1 conjugation of the adenovirus E1B-55K protein during productive infection. Our data show that CRM1 is the major export...

Inhibition of Cd83 Cell Surface Expression during Dendritic Cell Maturation by Interference with Nuclear Export of Cd83 mRNA

Kruse, Monika, Rosorius, Olaf, Krätzer, Friedrich, Bevec, Dorian, Kuhnt, Christine, Steinkasserer, Alexander, ...

Dendritic cells (DCs), nature's adjuvant, must mature to sensitize T cells. However, although the maturation process is essential, it is not yet fully understood at the molecular level. In this...

Cofactor Requirements for Nuclear Export of Rev Response Element (Rre)–And Constitutive Transport Element (Cte)–Containing Retroviral Rnas: An Unexpected Role for Actin

Hofmann, Wilma, Reichart, Beate, Ewald, Andrea, Müller, Eleonora, Schmitt, Iris, Stauber, Roland H., ...

Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the export receptor CRM1/exportin1. However, additional protein factors interacting with leucine-rich...