Investigation of mRNA Splicing in Saccharomyces cerevisiae using Microarrays (2005)
Barrass, J David, Beggs, Jean D
No abstract available.
Yeast Sm-like proteins function in mRNA decapping and decay (2000)
Tharun, Sundaresan, He, Weihai, Mayes, Andrew E., Lennertz, Pascal, Beggs, Jean D, Parker, Roy
One of the main mechanisms of messenger RNA degradation in eukaryotes occurs by deadenylation-dependent decapping which leads to 5'-to-3' decay1, 2. A family of Sm-like (Lsm) proteins has been...
Yeast Sm-like proteins function in mRNA decapping and decay (2000)
Tharun, Sundaresan, He, Weihai, Mayes, Andrew E., Lennertz, Pascal, Beggs, Jean D, Parker, Roy
One of the main mechanisms of messenger RNA degradation in eukaryotes occurs by deadenylation-dependent decapping which leads to 5'-to-3' decay1, 2. A family of Sm-like (Lsm) proteins has been...
Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3
Colley, Alan, Beggs, Jean D., Tollervey, David, Lafontaine, Denis L. J.
Putative RNA helicases are involved in most aspects of gene expression. All previously characterized members of the DEAH-box family of putative RNA helicases are involved in pre-mRNA splicing. Here...
Kufel, Joanna, Allmang, Christine, Verdone, Loredana, Beggs, Jean D., Tollervey, David
Depletion of any of the five essential proteins Lsm2p to Lsm5p and Lsm8p leads to strong accumulation of all tested unspliced pre-tRNA species, as well as accumulation of 5′ and 3′ unprocessed...
Nuclear Pre-mRNA Decapping and 5′ Degradation in Yeast Require the Lsm2-8p Complex
Kufel, Joanna, Bousquet-Antonelli, Cecile, Beggs, Jean D., Tollervey, David
Previous analyses have identified related cytoplasmic Lsm1-7p and nuclear Lsm2-8p complexes. Here we report that mature heat shock and MET mRNAs that are trapped in the nucleus due to a block in mRNA...
Silverman, Edward J., Maeda, Ayaka, Wei, Janet, Smith, Paul, Beggs, Jean D., Lin, Ren-Jang
Prp2 is an RNA-dependent ATPase that activates the spliceosome before the first transesterification reaction of pre-mRNA splicing. Prp2 has extensive homology throughout the helicase domain...
Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors
ALBERS, MICHAEL, DIMENT, ANN, MURARU, MARIELA, RUSSELL, CAROLINE S., BEGGS, JEAN D.
Through exhaustive two-hybrid screens using a budding yeast genomic library, and starting with the splicing factor and DEAH-box RNA helicase Prp22p as bait, we identified yeast Prp45p and Prp46p. We...
Prp8 protein: At the heart of the spliceosome
GRAINGER, RICHARD J., BEGGS, JEAN D.
Pre-messenger RNA (pre-mRNA) splicing is a central step in gene expression. Lying between transcription and protein synthesis, pre-mRNA splicing removes sequences (introns) that would otherwise...
Prp8p dissection reveals domain structure and protein interaction sites
BOON, KUM-LOONG, NORMAN, CHRISTINE M., GRAINGER, RICHARD J., NEWMAN, ANDREW J., BEGGS, JEAN D.
We describe a novel approach to characterize the functional domains of a protein in vivo. This involves the use of a custom-built Tn5-based transposon that causes the expression of a target gene as...
Yeast Ntr1/Spp382 Mediates Prp43 Function in Postspliceosomes
Boon, Kum-Loong, Auchynnikava, Tatsiana, Edwalds-Gilbert, Gretchen, Barrass, J. David, Droop, Alastair P., Dez, Christophe, ...
The Ntr1 and Ntr2 proteins of Saccharomyces cerevisiae have been reported to interact with proteins involved in pre-mRNA splicing, but their roles in the splicing process are unknown. We show here...
Dhr1p, a Putative DEAH-Box RNA Helicase, Is Associated with the Box C+D snoRNP U3
Colley, Alan, Beggs, Jean D., Tollervey, David, Lafontaine, Denis L. J.
Putative RNA helicases are involved in most aspects of gene expression. All previously characterized members of the DEAH-box family of putative RNA helicases are involved in pre-mRNA splicing. Here...
Kufel, Joanna, Allmang, Christine, Verdone, Loredana, Beggs, Jean D., Tollervey, David
Depletion of any of the five essential proteins Lsm2p to Lsm5p and Lsm8p leads to strong accumulation of all tested unspliced pre-tRNA species, as well as accumulation of 5′ and 3′ unprocessed...
Nuclear Pre-mRNA Decapping and 5′ Degradation in Yeast Require the Lsm2-8p Complex
Kufel, Joanna, Bousquet-Antonelli, Cecile, Beggs, Jean D., Tollervey, David
Previous analyses have identified related cytoplasmic Lsm1-7p and nuclear Lsm2-8p complexes. Here we report that mature heat shock and MET mRNAs that are trapped in the nucleus due to a block in mRNA...
Silverman, Edward J., Maeda, Ayaka, Wei, Janet, Smith, Paul, Beggs, Jean D., Lin, Ren-Jang
Prp2 is an RNA-dependent ATPase that activates the spliceosome before the first transesterification reaction of pre-mRNA splicing. Prp2 has extensive homology throughout the helicase domain...
Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors
ALBERS, MICHAEL, DIMENT, ANN, MURARU, MARIELA, RUSSELL, CAROLINE S., BEGGS, JEAN D.
Through exhaustive two-hybrid screens using a budding yeast genomic library, and starting with the splicing factor and DEAH-box RNA helicase Prp22p as bait, we identified yeast Prp45p and Prp46p. We...
Prp8 protein: At the heart of the spliceosome
GRAINGER, RICHARD J., BEGGS, JEAN D.
Pre-messenger RNA (pre-mRNA) splicing is a central step in gene expression. Lying between transcription and protein synthesis, pre-mRNA splicing removes sequences (introns) that would otherwise...
Prp8p dissection reveals domain structure and protein interaction sites
BOON, KUM-LOONG, NORMAN, CHRISTINE M., GRAINGER, RICHARD J., NEWMAN, ANDREW J., BEGGS, JEAN D.
We describe a novel approach to characterize the functional domains of a protein in vivo. This involves the use of a custom-built Tn5-based transposon that causes the expression of a target gene as...
Yeast Ntr1/Spp382 Mediates Prp43 Function in Postspliceosomes
Boon, Kum-Loong, Auchynnikava, Tatsiana, Edwalds-Gilbert, Gretchen, Barrass, J. David, Droop, Alastair P., Dez, Christophe, ...
The Ntr1 and Ntr2 proteins of Saccharomyces cerevisiae have been reported to interact with proteins involved in pre-mRNA splicing, but their roles in the splicing process are unknown. We show here...
The Lsm2-8 complex determines nuclear localization of the spliceosomal U6 snRNA
Spiller, Michael P., Boon, Kum-Loong, Reijns, Martin A. M., Beggs, Jean D.
Lsm proteins are ubiquitous, multifunctional proteins that are involved in the processing and/or turnover of many, if not all, RNAs in eukaryotes. They generally interact only transiently with their...
Genome-Wide Protein Interaction Screens Reveal Functional Networks Involving Sm-Like Proteins
Fromont-Racine, Micheline, Mayes, Andrew E., Brunet-Simon, Adeline, Rain, Jean-Christophe, Colley, Alan, Dix, Ian, ...
A set of seven structurally related Sm proteins forms the core of the snRNP particles containing the spliceosomal U1, U2, U4 and U5 snRNAs. A search of the genomic sequence of Saccharomyces...