Correlation between CpG methylation profiles and hormone receptor status in breast cancers (2007)
Feng, Weiwei, Shen, Lanlan, Wen, Sijin, Rosen, Daniel G, Jelinek, Jaroslav, Hu, Xin, ...
Abstract Introduction Aberrant DNA methylation has been found frequently in human breast cancers, associated with the loss of expression of a number of regulatory genes for growth and correlated to...
Epigenetic Silencing and Resistance to Imatinib Mesylate in CML (1998)
Resistance to Imatinib mesylate is emerging as a real clinical problem in the management of chronic myelogenous leukemia (CML). In this project, we are exploring the hypothesis that epigenetic...
Herman, James G., Umar, Asad, Polyak, Kornelia, Graff, Jeremy R., Ahuja, Nita, Issa, Jean-Pierre J., ...
Inactivation of the genes involved in DNA mismatch repair is associated with microsatellite instability (MSI) in colorectal cancer. We report that hypermethylation of the 5' CpG island of hMLH1 is...
Distinct genetic profiles in colorectal tumors with or without the CpG island methylator phenotype
Toyota, Minoru, Ohe-Toyota, Mutsumi, Ahuja, Nita, Issa, Jean-Pierre J.
Colorectal cancers (CRCs) are characterized by multiple genetic (mutations) and epigenetic (CpG island methylation) alterations, but it is not known whether these evolve independently through...
CpG island methylator phenotype in colorectal cancer
Toyota, Minoru, Ahuja, Nita, Ohe-Toyota, Mutsumi, Herman, James G., Baylin, Stephen B., Issa, Jean-Pierre J.
Aberrant methylation of promoter region CpG islands is associated with transcriptional inactivation of tumor-suppressor genes in neoplasia. To understand global patterns of CpG island methylation in...
Incidence and functional consequences of hMLH1 promoter hypermethylation in colorectal carcinoma
Herman, James G., Umar, Asad, Polyak, Kornelia, Graff, Jeremy R., Ahuja, Nita, Issa, Jean-Pierre J., ...
Inactivation of the genes involved in DNA mismatch repair is associated with microsatellite instability (MSI) in colorectal cancer. We report that hypermethylation of the 5′ CpG island of hMLH1 is...
Mikovits, Judy A., Young, Howard A., Vertino, Paula, Issa, Jean-Pierre J., Pitha, Paula M., Turcoski-Corrales, Susan, ...
The immune response to pathogens is regulated by a delicate balance of cytokines. The dysregulation of cytokine gene expression, including interleukin-12, tumor necrosis factor alpha, and gamma...
Critical Role of Histone Methylation in Tumor Suppressor Gene Silencing in Colorectal Cancer
Kondo, Yutaka, Shen, LanLan, Issa, Jean-Pierre J.
The mechanism of DNA hypermethylation-associated tumor suppressor gene silencing in cancer remains incompletely understood. Here, we show by chromatin immunoprecipitation that for three genes (P16,...
Epigenetic inactivation of CHFR in human tumors
Toyota, Minoru, Sasaki, Yasushi, Satoh, Ayumi, Ogi, Kazuhiro, Kikuchi, Takefumi, Suzuki, Hiromu, ...
Cell-cycle checkpoints controlling the orderly progression through mitosis are frequently disrupted in human cancers. One such checkpoint, entry into metaphase, is regulated by the CHFR gene encoding...
A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements
Yang, Allen S., Estécio, Marcos R. H., Doshi, Ketan, Kondo, Yutaka, Tajara, Eloiza H., Issa, Jean-Pierre J.
We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed...
Kondo, Yutaka, Shen, Lanlan, Yan, Pearlly S., Huang, Tim Hui-Ming, Issa, Jean-Pierre J.
Switching from acetylation to methylation at histone H3 lysine 9 (K9) has recently been shown to contribute to euchromatin gene silencing. To identify genes silenced by K9 modifications, we probed a...
Distinct genetic profiles in colorectal tumors with or without the CpG island methylator phenotype
Toyota, Minoru, Ohe-Toyota, Mutsumi, Ahuja, Nita, Issa, Jean-Pierre J.
Colorectal cancers (CRCs) are characterized by multiple genetic (mutations) and epigenetic (CpG island methylation) alterations, but it is not known whether these evolve independently through...
CpG island methylator phenotype in colorectal cancer
Toyota, Minoru, Ahuja, Nita, Ohe-Toyota, Mutsumi, Herman, James G., Baylin, Stephen B., Issa, Jean-Pierre J.
Aberrant methylation of promoter region CpG islands is associated with transcriptional inactivation of tumor-suppressor genes in neoplasia. To understand global patterns of CpG island methylation in...
Incidence and functional consequences of hMLH1 promoter hypermethylation in colorectal carcinoma
Herman, James G., Umar, Asad, Polyak, Kornelia, Graff, Jeremy R., Ahuja, Nita, Issa, Jean-Pierre J., ...
Inactivation of the genes involved in DNA mismatch repair is associated with microsatellite instability (MSI) in colorectal cancer. We report that hypermethylation of the 5′ CpG island of hMLH1 is...
Mikovits, Judy A., Young, Howard A., Vertino, Paula, Issa, Jean-Pierre J., Pitha, Paula M., Turcoski-Corrales, Susan, ...
The immune response to pathogens is regulated by a delicate balance of cytokines. The dysregulation of cytokine gene expression, including interleukin-12, tumor necrosis factor alpha, and gamma...
Critical Role of Histone Methylation in Tumor Suppressor Gene Silencing in Colorectal Cancer
Kondo, Yutaka, Shen, LanLan, Issa, Jean-Pierre J.
The mechanism of DNA hypermethylation-associated tumor suppressor gene silencing in cancer remains incompletely understood. Here, we show by chromatin immunoprecipitation that for three genes (P16,...
Epigenetic inactivation of CHFR in human tumors
Toyota, Minoru, Sasaki, Yasushi, Satoh, Ayumi, Ogi, Kazuhiro, Kikuchi, Takefumi, Suzuki, Hiromu, ...
Cell-cycle checkpoints controlling the orderly progression through mitosis are frequently disrupted in human cancers. One such checkpoint, entry into metaphase, is regulated by the CHFR gene encoding...
A simple method for estimating global DNA methylation using bisulfite PCR of repetitive DNA elements
Yang, Allen S., Estécio, Marcos R. H., Doshi, Ketan, Kondo, Yutaka, Tajara, Eloiza H., Issa, Jean-Pierre J.
We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed...
Kondo, Yutaka, Shen, Lanlan, Yan, Pearlly S., Huang, Tim Hui-Ming, Issa, Jean-Pierre J.
Switching from acetylation to methylation at histone H3 lysine 9 (K9) has recently been shown to contribute to euchromatin gene silencing. To identify genes silenced by K9 modifications, we probed a...
BRAF Mutations in Aberrant Crypt Foci and Hyperplastic Polyposis
Beach, Robyn, Chan, Annie On-On, Wu, Tsung-Teh, White, Jill A., Morris, Jeffrey S., Lunagomez, Simon, ...
Patients with hyperplastic polyposis have multiple hyperplastic polyps (HPs) and increased risk of colorectal carcinomas. Aberrant crypt foci (ACF) are postulated to be the earliest precursor lesions...
Estécio, Marcos R.H., Gharibyan, Vazganush, Shen, Lanlan, Ibrahim, Ashraf E.K., Doshi, Ketan, He, Rong, ...
CpG Island Methylation in Colorectal Adenomas
Rashid, Asif, Shen, Lanlan, Morris, Jeffrey S., Issa, Jean-Pierre J., Hamilton, Stanley R.
Methylation of cytosines in CpG islands silences gene expression. CpG island methylator phenotype (CIMP) in colorectal cancers is characterized by abnormal methylation of multiple CpG islands...
Concordant CpG Island Methylation in Hyperplastic Polyposis
Chan, Annie On-On, Issa, Jean-Pierre J., Morris, Jeffrey S., Hamilton, Stanley R., Rashid, Asif
The CpG island methylator phenotype (CIMP) is a newly described mechanism for carcinogenesis in colorectal carcinomas and adenomas characterized by methylation of multiple CpG islands. The causes of...
CpG Island Methylation in Aberrant Crypt Foci of the Colorectum
Chan, Annie On-On, Broaddus, Russell R., Houlihan, Patrick S., Issa, Jean-Pierre J., Hamilton, Stanley R., Rashid, Asif
Aberrant crypt foci (ACF) are postulated to be the earliest precursor lesion in colorectal carcinogenesis, and CpG island methylation has been described as an important molecular pathway. We...
Jelinek, Jaroslav, Oki, Yasuhiro, Gharibyan, Vazganush, Bueso-Ramos, Carlos, Prchal, Josef T., Verstovsek, Srdan, ...
An activating 1849G>T mutation of JAK2 (Janus kinase 2) tyrosine kinase was recently described in chronic myeloproliferative disorders (MPDs). Its role in other hematologic neoplasms is unclear. We...
Genome-Wide Profiling of DNA Methylation Reveals a Class of Normally Methylated CpG Island Promoters
Shen, Lanlan, Kondo, Yutaka, Guo, Yi, Zhang, Jiexin, Zhang, Li, Ahmed, Saira, ...
The role of CpG island methylation in normal development and cell differentiation is of keen interest, but remains poorly understood. We performed comprehensive DNA methylation profiling of promoter...
Correlation between CpG methylation profiles and hormone receptor status in breast cancers
Feng, Weiwei, Shen, Lanlan, Wen, Sijin, Rosen, Daniel G, Jelinek, Jaroslav, Hu, Xin, ...
High-throughput methylation profiling by MCA coupled to CpG island microarray
Estécio, Marcos R.H., Yan, Pearlly S., Ibrahim, Ashraf E.K., Tellez, Carmen S., Shen, Lanlan, Huang, Tim H.-M., ...
An abnormal pattern of DNA methylation occurs at specific genes in almost all neoplasms. The lack of high-throughput methods with high specificity and sensitivity to detect changes in DNA methylation...
Kondo, Yutaka, Shen, Lanlan, Ahmed, Saira, Boumber, Yanis, Sekido, Yoshitaka, Haddad, Bassem R., ...
Chung, Woonbok, Kwabi-Addo, Bernard, Ittmann, Michael, Jelinek, Jaroslav, Shen, Lanlan, Yu, Yinhua, ...
DNA hypermethylation is a common epigenetic abnormality in cancer and may serve as a useful marker to clone cancer-related genes as well as a marker of clinical disease activity. To identify CpG...
Integrated genetic and epigenetic analysis identifies three different subclasses of colon cancer
Shen, Lanlan, Toyota, Minoru, Kondo, Yutaka, Lin, E, Zhang, Li, Guo, Yi, ...
Colon cancer has been viewed as the result of progressive accumulation of genetic and epigenetic abnormalities. However, this view does not fully reflect the molecular heterogeneity of the disease....
An Sp1/Sp3 Binding Polymorphism Confers Methylation Protection
Boumber, Yanis A., Kondo, Yutaka, Chen, Xuqi, Shen, Lanlan, Guo, Yi, Tellez, Carmen, ...
Hundreds of genes show aberrant DNA hypermethylation in cancer, yet little is known about the causes of this hypermethylation. We identified RIL as a frequent methylation target in cancer. In search...