Simonet, Valérie, Malléa, Monique, Pagès, Jean-Marie
The Escherichia coli OmpF porin is a nonspecific channel involved in the membrane translocation of small hydrophilic molecules and especially in the passage of β-lactam antibiotics. In order to...
Dedieu, Luc, Pagès, Jean-Marie, Bolla, Jean-Michel
Porins allow exchanges between bacteria and their environment. In the gram-negative food-borne pathogen Campylobacter jejuni two porins, major outer membrane protein (MOMP) and Omp50, have been...
mar Operon Involved in Multidrug Resistance of Enterobacter aerogenes
Chollet, Renaud, Bollet, Claude, Chevalier, Jacqueline, Malléa, Monique, Pagès, Jean-Marie, Davin-Regli, Anne
We determined the sequence of the entire marRAB operon in Enterobacter aerogenes. It is functionally and structurally analogous to the Escherichia coli operon. The overexpression of E. aerogenes MarA...
Pradel, Elizabeth, Pagès, Jean-Marie
We identified the genes encoding the AcrA-AcrB-TolC efflux pump in Enterobacter aerogenes and constructed acrAB and tolC mutants from a multidrug-resistant isolate. Both derivatives were more...
Gayet, Stéphane, Chollet, Renaud, Molle, Gérard, Pagès, Jean-Marie, Chevalier, Jacqueline
Two clinical strains of Enterobacter aerogenes that exhibited phenotypes of multiresistance to β-lactam antibiotics, fluoroquinolones, chloramphenicol, tetracycline, and kanamycin were investigated....
Chevalier, Jacqueline, Bredin, Jérôme, Mahamoud, Abdallah, Malléa, Monique, Barbe, Jacques, Pagès, Jean-Marie
In Enterobacter aerogenes and Klebsiella pneumoniae, efflux provides efficient extrusion of antibiotics and contributes to the multidrug resistance phenotype. One of the alkoxyquinoline derivatives...
Use of the omp50 Gene for Identification of Campylobacter Species by PCR
Dedieu, Luc, Pagès, Jean-Marie, Bolla, Jean-Michel
We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and...
Omp35, a New Enterobacter aerogenes Porin Involved in Selective Susceptibility to Cephalosporins
Bornet, Charléric, Saint, Nathalie, Fetnaci, Lilia, Dupont, Myrielle, Davin-Régli, Anne, Bollet, Claude, ...
In Enterobacter aerogenes, β-lactam resistance often involves a decrease in outer membrane permeability induced by modifications of porin synthesis. In ATCC 15038 strain, we observed a different...
Hasdemir, Ufuk Over, Chevalier, Jacqueline, Nordmann, Patrice, Pagès, Jean-Marie
The prevalence of active drug efflux pump and porin alterations was investigated in Turkish nosocomial strains of Klebsiella pneumoniae exhibiting a multidrug-resistant phenotype. MICs of various...
Chollet, Renaud, Chevalier, Jacqueline, Bryskier, André, Pagès, Jean-Marie
The role of the AcrAB-TolC pump in macrolide and ketolide susceptibility in Escherichia coli and Enterobacter aerogenes was studied. Efflux pump inhibitor restored erythromycin, clarithromycin, and...
Thiolas, Aurélie, Bollet, Claude, La Scola, Bernard, Raoult, Didier, Pagès, Jean-Marie
Enterobacter aerogenes is an agent of hospital-acquired infection that exhibits a remarkable resistance to β-lactam antibiotics during therapy. Five successive isolates of E. aerogenes infecting a...
The eefABC Multidrug Efflux Pump Operon Is Repressed by H-NS in Enterobacter aerogenes
Masi, Muriel, Pagès, Jean-Marie, Villard, Claude, Pradel, Elizabeth
The Enterobacter aerogenes eefABC locus, which encodes a tripartite efflux pump, was cloned by complementation of an Escherichia coli tolC mutant. E. aerogenes ΔacrA expressing EefABC became less...
Bredin, Jérôme, Saint, Nathalie, Malléa, Monique, D, Emmanuelle, Molle, Gérard, Pagès, Jean-Marie, ...
The Escherichia coli OmpF pore is governed by an internal constriction consisting of the negatively charged loop 3 folded into the lumen and the positively charged barrel wall located on the opposite...
Colicins, spermine and cephalosporins: a competitive interaction with the OmpF eyelet.
Bredin, Jérôme, Simonet, Valérie, Iyer, Ramkumar, Delcour, Anne H, Pagès, Jean-Marie
The L3 loop is an important feature of the OmpF porin structure, contributing to both channel size and electrostatic properties. Colicins A and N, spermine, and antibiotics that use OmpF to penetrate...
Malléa, Monique, Mahamoud, Abdallah, Chevalier, Jacqueline, Alibert-Franco, Sandrine, Brouant, Pierre, Barbe, Jacques, ...
Over the last decade, MDR (multidrug resistance) has increased worldwide in microbial pathogens by efflux mechanisms, leading to treatment failures in human infections. Several Gram-negative bacteria...
Goulhen, Florence, Dé, Emmanuelle, Pagès, Jean-Marie, Bolla, Jean-Michel
Functional and structural studies of outer membrane proteins from Gram-negative bacteria are frequently carried out using refolded proteins. Recombinant proteins are produced in Escherichia coli as...
Braibant, Martine, Chevalier, Jacqueline, Chaslus-Dancla, Elisabeth, Pagès, Jean-Marie, Cloeckaert, Axel
The florfenicol-chloramphenicol resistance gene floR from Salmonella enterica was previously identified and postulated to belong to the major facilitator (MF) superfamily of drug exporters. Here, we...
Simonet, Valérie, Malléa, Monique, Pagès, Jean-Marie
The Escherichia coli OmpF porin is a nonspecific channel involved in the membrane translocation of small hydrophilic molecules and especially in the passage of β-lactam antibiotics. In order to...
Dedieu, Luc, Pagès, Jean-Marie, Bolla, Jean-Michel
Porins allow exchanges between bacteria and their environment. In the gram-negative food-borne pathogen Campylobacter jejuni two porins, major outer membrane protein (MOMP) and Omp50, have been...
mar Operon Involved in Multidrug Resistance of Enterobacter aerogenes
Chollet, Renaud, Bollet, Claude, Chevalier, Jacqueline, Malléa, Monique, Pagès, Jean-Marie, Davin-Regli, Anne
We determined the sequence of the entire marRAB operon in Enterobacter aerogenes. It is functionally and structurally analogous to the Escherichia coli operon. The overexpression of E. aerogenes MarA...
Pradel, Elizabeth, Pagès, Jean-Marie
We identified the genes encoding the AcrA-AcrB-TolC efflux pump in Enterobacter aerogenes and constructed acrAB and tolC mutants from a multidrug-resistant isolate. Both derivatives were more...
Gayet, Stéphane, Chollet, Renaud, Molle, Gérard, Pagès, Jean-Marie, Chevalier, Jacqueline
Two clinical strains of Enterobacter aerogenes that exhibited phenotypes of multiresistance to β-lactam antibiotics, fluoroquinolones, chloramphenicol, tetracycline, and kanamycin were investigated....
Chevalier, Jacqueline, Bredin, Jérôme, Mahamoud, Abdallah, Malléa, Monique, Barbe, Jacques, Pagès, Jean-Marie
In Enterobacter aerogenes and Klebsiella pneumoniae, efflux provides efficient extrusion of antibiotics and contributes to the multidrug resistance phenotype. One of the alkoxyquinoline derivatives...
Use of the omp50 Gene for Identification of Campylobacter Species by PCR
Dedieu, Luc, Pagès, Jean-Marie, Bolla, Jean-Michel
We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and...
Omp35, a New Enterobacter aerogenes Porin Involved in Selective Susceptibility to Cephalosporins
Bornet, Charléric, Saint, Nathalie, Fetnaci, Lilia, Dupont, Myrielle, Davin-Régli, Anne, Bollet, Claude, ...
In Enterobacter aerogenes, β-lactam resistance often involves a decrease in outer membrane permeability induced by modifications of porin synthesis. In ATCC 15038 strain, we observed a different...
Hasdemir, Ufuk Over, Chevalier, Jacqueline, Nordmann, Patrice, Pagès, Jean-Marie
The prevalence of active drug efflux pump and porin alterations was investigated in Turkish nosocomial strains of Klebsiella pneumoniae exhibiting a multidrug-resistant phenotype. MICs of various...
Chollet, Renaud, Chevalier, Jacqueline, Bryskier, André, Pagès, Jean-Marie
The role of the AcrAB-TolC pump in macrolide and ketolide susceptibility in Escherichia coli and Enterobacter aerogenes was studied. Efflux pump inhibitor restored erythromycin, clarithromycin, and...
Thiolas, Aurélie, Bollet, Claude, La Scola, Bernard, Raoult, Didier, Pagès, Jean-Marie
Enterobacter aerogenes is an agent of hospital-acquired infection that exhibits a remarkable resistance to β-lactam antibiotics during therapy. Five successive isolates of E. aerogenes infecting a...
The eefABC Multidrug Efflux Pump Operon Is Repressed by H-NS in Enterobacter aerogenes
Masi, Muriel, Pagès, Jean-Marie, Villard, Claude, Pradel, Elizabeth
The Enterobacter aerogenes eefABC locus, which encodes a tripartite efflux pump, was cloned by complementation of an Escherichia coli tolC mutant. E. aerogenes ΔacrA expressing EefABC became less...
Braibant, Martine, Chevalier, Jacqueline, Chaslus-Dancla, Elisabeth, Pagès, Jean-Marie, Cloeckaert, Axel
The florfenicol-chloramphenicol resistance gene floR from Salmonella enterica was previously identified and postulated to belong to the major facilitator (MF) superfamily of drug exporters. Here, we...
Bredin, Jérôme, Saint, Nathalie, Malléa, Monique, D, Emmanuelle, Molle, Gérard, Pagès, Jean-Marie, ...
The Escherichia coli OmpF pore is governed by an internal constriction consisting of the negatively charged loop 3 folded into the lumen and the positively charged barrel wall located on the opposite...
Colicins, spermine and cephalosporins: a competitive interaction with the OmpF eyelet.
Bredin, Jérôme, Simonet, Valérie, Iyer, Ramkumar, Delcour, Anne H, Pagès, Jean-Marie
The L3 loop is an important feature of the OmpF porin structure, contributing to both channel size and electrostatic properties. Colicins A and N, spermine, and antibiotics that use OmpF to penetrate...
Malléa, Monique, Mahamoud, Abdallah, Chevalier, Jacqueline, Alibert-Franco, Sandrine, Brouant, Pierre, Barbe, Jacques, ...
Over the last decade, MDR (multidrug resistance) has increased worldwide in microbial pathogens by efflux mechanisms, leading to treatment failures in human infections. Several Gram-negative bacteria...
Goulhen, Florence, Dé, Emmanuelle, Pagès, Jean-Marie, Bolla, Jean-Michel
Functional and structural studies of outer membrane proteins from Gram-negative bacteria are frequently carried out using refolded proteins. Recombinant proteins are produced in Escherichia coli as...
Antibiotic Stress, Genetic Response and Altered Permeability of E. coli
Viveiros, Miguel, Dupont, Myrielle, Rodrigues, Liliana, Couto, Isabel, Davin-Regli, Anne, Martins, Marta, ...
Dupont, Myrielle, James, Chloë E., Chevalier, Jacqueline, Pagès, Jean-Marie
Bacterial adaptation to external stresses and toxic compounds is a key step in the emergence of multidrug-resistant strains that are a serious threat to human health. Although some of the proteins...
Salmi, Chanaz, Loncle, Celine, Vidal, Nicolas, Letourneux, Yves, Fantini, Jacques, Maresca, Marc, ...
We reported that squalamine is a membrane-active molecule that targets the membrane integrity as demonstrated by the ATP release and dye entry. In this context, its activity may depend on the...