Depp, Sebastien, Rochaix, Jean-David, Goldschmidt-Clermont, Michel
Ce travail de thèse concerne l'étude de l'expression des gènes chloroplastiques "psbB/psbT/psbH" chez "Chlamydomonas reinhardtii". Une première série de huits mutations successives dans...
Depp, Sebastien, Rochaix, Jean-David, Goldschmidt-Clermont, Michel
Ce travail de thèse concerne l'étude de l'expression des gènes chloroplastiques "psbB/psbT/psbH" chez "Chlamydomonas reinhardtii". Une première série de huits mutations successives dans...
Vaistij, Fabián E., Boudreau, Eric, Lemaire, Stéphane D., Goldschmidt-Clermont, Michel, Rochaix, Jean-David
Genetic analysis has revealed that the accumulation of several chloroplast mRNAs of the green alga Chlamydomonas reinhardtii requires specific nucleus-encoded functions. To gain insight into this...
Hippler, Michael, Drepper, Friedel, Haehnel, Wolfgang, Rochaix, Jean-David
The PsaF-deficient mutant 3bF of Chlamydomonas reinhardtii was used to modify PsaF by nuclear transformation and site-directed mutagenesis. Four lysine residues in the N-terminal domain of PsaF,...
Fleischmann, Mark M., Rochaix, Jean-David
We have changed the potential phosphorylation site, a threonine residue at position 2 of the D2 polypeptide of the photosystem II complex of Chlamydomonas reinhardtii, to alanine, valine, aspartate,...
Naver, Helle, Boudreau, Eric, Rochaix, Jean-David
The Ycf3 protein is essential for the accumulation of the photosystem I (PSI) complex and acts at a post-translational level. The sequence of Ycf3 is conserved in cyanobacteria, algae, and plants and...
Rivier, Cristian, Goldschmidt-Clermont, Michel, Rochaix, Jean-David
In Chlamydomonas reinhardtii, the psaA mRNA is assembled by a process involving trans-splicing of separate transcripts, encoded at three separate loci of the chloroplast genome. At least 14 nuclear...
Loss of Albino3 Leads to the Specific Depletion of the Light-Harvesting System
Bellafiore, Stéphane, Ferris, Patrick, Naver, Helle, Göhre, Vera, Rochaix, Jean-David
The chloroplast Albino3 (Alb3) protein is a chloroplast homolog of the mitochondrial Oxa1p and YidC proteins of Escherichia coli, which are essential components for integrating membrane proteins. In...
Tab2 is a novel conserved RNA binding protein required for translation of the chloroplast psaB mRNA
Dauvillée, David, Stampacchia, Otello, Girard-Bascou, Jacqueline, Rochaix, Jean-David
The chloroplast psaB mRNA encodes one of the reaction centre polypeptides of photosystem I. Protein pulse-labelling profiles indicate that the mutant strain of Chlamydomonas reinhardtii, F14,...
Boudreau, Eric, Nickelsen, Jörg, Lemaire, Stéphane D., Ossenbühl, Friedrich, Rochaix, Jean-David
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2...
Erickson, Jeanne M., Rahire, Michèle, Bennoun, Pierre, Delepelaire, Philippe, Diner, Bruce, Rochaix, Jean-David
We report the isolation and characterization of a uniparental mutant of Chlamydomonas reinhardtii that is resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and...
The Amplified Ribosomal DNA of Dytiscid Beetles
Gall, Joseph G., Rochaix, Jean-David
During oogenesis in many animals there is a massive extrachromosomal synthesis of the genes for ribosomal RNA. In Dytiscid beetles, as in the toad Xenopus, the amplified ribosomal DNA occurs as...
Spreitzer, Robert J., Goldschmidt-Clermont, Michel, Rahire, Michele, Rochaix, Jean-David
The Chlamydomonas reinhardtii chloroplast mutants 18-5B and 18-7G lack both the chloroplast-encoded large subunit and nuclear-encoded small subunit of the chloroplast enzyme ribulose-1,5-bisphosphate...
Ossenbühl, Friedrich, Göhre, Vera, Meurer, Jörg, Krieger-Liszkay, Anja, Rochaix, Jean-David, Eichacker, Lutz A.
Alb3 homologs Oxa1 and YidC have been shown to be required for the integration of newly synthesized proteins into membranes. Here, we show that although Alb3.1p is not required for integration of the...
Falciatore, Angela, Merendino, Livia, Barneche, Fredy, Ceol, Mauro, Meskauskiene, Rasa, Apel, Klaus, ...
In photosynthetic organisms the accumulation of harmful photodynamic chlorophyll precursors is prevented because of the tight regulation of the tetrapyrrole pathway. FLU is one of the regulatory...
Erickson, Jeanne M., Rahire, Michèle, Rochaix, Jean-David
The chloroplast psbA gene from the green unicellular alga Chlamydomonas reinhardii has been localized, cloned and sequenced. This gene codes for the rapidly-labeled 32-kd protein of photosystem II,...
Finazzi, Giovanni, Rappaport, Fabrice, Furia, Alberto, Fleischmann, Mark, Rochaix, Jean-David, Zito, Francesca, ...
The energetic metabolism of photosynthetic organisms is profoundly influenced by state transitions and cyclic electron flow around photosystem I. The former involve a reversible redistribution of the...
Hurt, Eduard C., Soltanifar, Nouchine, Goldschmidt-Clermont, Michel, Rochaix, Jean-David, Schatz, Gottfried
The cleavable pre-sequences of imported chloroplast and mitochondrial proteins have several features in common. This structural similarity prompted us to test whether a chloroplast pre-sequence...
Erickson, Jeanne M., Rahire, Michéle, Malnoë, Pia, Girard-Bascou, Jacqueline, Pierre, Yves, Bennoun, Pierre, ...
D1 and D2, two chloroplast proteins with apparent mol. wt of 32 000-34 000, play an important role in the photosynthetic reactions mediated by the membrane-bound protein complex of photosystem II...
A novel multifunctional factor involved in trans-splicing of chloroplast introns in Chlamydomonas
Merendino, Livia, Perron, Karl, Rahire, Michèle, Howald, Isabelle, Rochaix, Jean-David, Goldschmidt-Clermont, Michel
In the chloroplast of Chlamydomonas reinhardtii, psaA mRNA is spliced in trans from three separate precursors which assemble to form two group II introns. A fourth transcript, tscA, completes the...
A multiprotein complex involved in chloroplast group II intron splicing
PERRON, KARL, GOLDSCHMIDT-CLERMONT, MICHEL, ROCHAIX, JEAN-DAVID
The psaA gene of the green alga Chlamydomonas reinhardtii consists of three exons that are widely separated on the chloroplast genome and transcribed independently. The exons are flanked by group II...
Zerges, William, Auchincloss, Andrea H, Rochaix, Jean-David
Translation of the chloroplast psbC mRNA in the unicellular eukaryotic alga Chlamydomonas reinhardtii is controlled by interactions between its 547-base 5' untranslated region and the products of the...
Vaistij, Fabián E., Boudreau, Eric, Lemaire, Stéphane D., Goldschmidt-Clermont, Michel, Rochaix, Jean-David
Genetic analysis has revealed that the accumulation of several chloroplast mRNAs of the green alga Chlamydomonas reinhardtii requires specific nucleus-encoded functions. To gain insight into this...
Hippler, Michael, Drepper, Friedel, Haehnel, Wolfgang, Rochaix, Jean-David
The PsaF-deficient mutant 3bF of Chlamydomonas reinhardtii was used to modify PsaF by nuclear transformation and site-directed mutagenesis. Four lysine residues in the N-terminal domain of PsaF,...
Fleischmann, Mark M., Rochaix, Jean-David
We have changed the potential phosphorylation site, a threonine residue at position 2 of the D2 polypeptide of the photosystem II complex of Chlamydomonas reinhardtii, to alanine, valine, aspartate,...
Naver, Helle, Boudreau, Eric, Rochaix, Jean-David
The Ycf3 protein is essential for the accumulation of the photosystem I (PSI) complex and acts at a post-translational level. The sequence of Ycf3 is conserved in cyanobacteria, algae, and plants and...
Rivier, Cristian, Goldschmidt-Clermont, Michel, Rochaix, Jean-David
In Chlamydomonas reinhardtii, the psaA mRNA is assembled by a process involving trans-splicing of separate transcripts, encoded at three separate loci of the chloroplast genome. At least 14 nuclear...
Loss of Albino3 Leads to the Specific Depletion of the Light-Harvesting System
Bellafiore, Stéphane, Ferris, Patrick, Naver, Helle, Göhre, Vera, Rochaix, Jean-David
The chloroplast Albino3 (Alb3) protein is a chloroplast homolog of the mitochondrial Oxa1p and YidC proteins of Escherichia coli, which are essential components for integrating membrane proteins. In...
Tab2 is a novel conserved RNA binding protein required for translation of the chloroplast psaB mRNA
Dauvillée, David, Stampacchia, Otello, Girard-Bascou, Jacqueline, Rochaix, Jean-David
The chloroplast psaB mRNA encodes one of the reaction centre polypeptides of photosystem I. Protein pulse-labelling profiles indicate that the mutant strain of Chlamydomonas reinhardtii, F14,...
Boudreau, Eric, Nickelsen, Jörg, Lemaire, Stéphane D., Ossenbühl, Friedrich, Rochaix, Jean-David
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2...
Erickson, Jeanne M., Rahire, Michèle, Bennoun, Pierre, Delepelaire, Philippe, Diner, Bruce, Rochaix, Jean-David
We report the isolation and characterization of a uniparental mutant of Chlamydomonas reinhardtii that is resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and...
The Amplified Ribosomal DNA of Dytiscid Beetles
Gall, Joseph G., Rochaix, Jean-David
During oogenesis in many animals there is a massive extrachromosomal synthesis of the genes for ribosomal RNA. In Dytiscid beetles, as in the toad Xenopus, the amplified ribosomal DNA occurs as...
Spreitzer, Robert J., Goldschmidt-Clermont, Michel, Rahire, Michele, Rochaix, Jean-David
The Chlamydomonas reinhardtii chloroplast mutants 18-5B and 18-7G lack both the chloroplast-encoded large subunit and nuclear-encoded small subunit of the chloroplast enzyme ribulose-1,5-bisphosphate...
Ossenbühl, Friedrich, Göhre, Vera, Meurer, Jörg, Krieger-Liszkay, Anja, Rochaix, Jean-David, Eichacker, Lutz A.
Alb3 homologs Oxa1 and YidC have been shown to be required for the integration of newly synthesized proteins into membranes. Here, we show that although Alb3.1p is not required for integration of the...
Falciatore, Angela, Merendino, Livia, Barneche, Fredy, Ceol, Mauro, Meskauskiene, Rasa, Apel, Klaus, ...
In photosynthetic organisms the accumulation of harmful photodynamic chlorophyll precursors is prevented because of the tight regulation of the tetrapyrrole pathway. FLU is one of the regulatory...
Erickson, Jeanne M., Rahire, Michèle, Rochaix, Jean-David
The chloroplast psbA gene from the green unicellular alga Chlamydomonas reinhardii has been localized, cloned and sequenced. This gene codes for the rapidly-labeled 32-kd protein of photosystem II,...
Finazzi, Giovanni, Rappaport, Fabrice, Furia, Alberto, Fleischmann, Mark, Rochaix, Jean-David, Zito, Francesca, ...
The energetic metabolism of photosynthetic organisms is profoundly influenced by state transitions and cyclic electron flow around photosystem I. The former involve a reversible redistribution of the...
Hurt, Eduard C., Soltanifar, Nouchine, Goldschmidt-Clermont, Michel, Rochaix, Jean-David, Schatz, Gottfried
The cleavable pre-sequences of imported chloroplast and mitochondrial proteins have several features in common. This structural similarity prompted us to test whether a chloroplast pre-sequence...
Erickson, Jeanne M., Rahire, Michéle, Malnoë, Pia, Girard-Bascou, Jacqueline, Pierre, Yves, Bennoun, Pierre, ...
D1 and D2, two chloroplast proteins with apparent mol. wt of 32 000-34 000, play an important role in the photosynthetic reactions mediated by the membrane-bound protein complex of photosystem II...
A novel multifunctional factor involved in trans-splicing of chloroplast introns in Chlamydomonas
Merendino, Livia, Perron, Karl, Rahire, Michèle, Howald, Isabelle, Rochaix, Jean-David, Goldschmidt-Clermont, Michel
In the chloroplast of Chlamydomonas reinhardtii, psaA mRNA is spliced in trans from three separate precursors which assemble to form two group II introns. A fourth transcript, tscA, completes the...
A multiprotein complex involved in chloroplast group II intron splicing
PERRON, KARL, GOLDSCHMIDT-CLERMONT, MICHEL, ROCHAIX, JEAN-DAVID
The psaA gene of the green alga Chlamydomonas reinhardtii consists of three exons that are widely separated on the chloroplast genome and transcribed independently. The exons are flanked by group II...
Zerges, William, Auchincloss, Andrea H, Rochaix, Jean-David
Translation of the chloroplast psbC mRNA in the unicellular eukaryotic alga Chlamydomonas reinhardtii is controlled by interactions between its 547-base 5' untranslated region and the products of the...
Göhre, Vera, Ossenbühl, Friedrich, Crèvecoeur, Michèle, Eichacker, Lutz Andreas, Rochaix, Jean-David
Proteins of the YidC/Oxa1p/ALB3 family play an important role in inserting proteins into membranes of mitochondria, bacteria, and chloroplasts. In Chlamydomonas reinhardtii, one member of this...
Barneche, Frédy, Winter, Veronika, Crèvecœur, Michèle, Rochaix, Jean-David
Plastid translational control depends to a large extent on the light conditions, and is presumably mediated by nucleus-encoded proteins acting on organelle gene expression. However, the molecular...
Potential for hydrogen production with inducible chloroplast gene expression in Chlamydomonas
Surzycki, Raymond, Cournac, Laurent, Peltier, Gilles, Rochaix, Jean-David
An inducible chloroplast gene expression system was developed in Chlamydomonas reinhardtii by taking advantage of the properties of the copper-sensitive cytochrome c6 promoter and of the...
Zerges, William, Rochaix, Jean-David
Chloroplast subfractions were tested with a UV cross-linking assay for proteins that bind to the 5′ untranslated region of the chloroplast psbC mRNA of the green alga Chlamydomonas reinhardtii....
Auchincloss, Andrea H., Zerges, William, Perron, Karl, Girard-Bascou, Jacqueline, Rochaix, Jean-David
Genetic analysis has revealed that the three nucleus-encoded factors Tbc1, Tbc2, and Tbc3 are involved in the translation of the chloroplast psbC mRNA of the eukaryotic green alga Chlamydomonas...