Allemand, Eric, Gattoni, Renata, Bourbon, Henri-Marc, Stevenin, James, Cáceres, Javier F., Soret, Johann, ...
The human splicing factor 2, also called human alternative splicing factor (hASF), is the prototype of the highly conserved SR protein family involved in constitutive and regulated splicing of...
Eperon, Ian C., Makarova, Olga V., Mayeda, Akila, Munroe, Stephen H., Cáceres, Javier F., Hayward, Daniel G., ...
The first component known to recognize and discriminate among potential 5′ splice sites (5′SSs) in pre-mRNA is the U1 snRNP. However, the relative levels of U1 snRNP binding to alternative...
Role of SR protein modular domains in alternative splicing specificity in vivo
Van Der Houven Van Oordt, Willemien, Newton, Kathryn, Screaton, Gavin R., Cáceres, Javier F.
The SR proteins constitute a family of nuclear phosphoproteins which are required for constitutive splicing and also influence alternative splicing regulation. They have a modular structure...
Nuclear Export and Retention Signals in the RS Domain of SR Proteins
Cazalla, Demian, Zhu, Jun, Manche, Lisa, Huber, Elisabeth, Krainer, Adrian R., Cáceres, Javier F.
Splicing factors of the SR protein family share a modular structure consisting of one or two RNA recognition motifs (RRMs) and a C-terminal RS domain rich in arginine and serine residues. The RS...
Newton, Kathryn, Petfalski, Elisabeth, Tollervey, David, Cáceres, Javier F.
Fibrillarin, a protein component of C/D box small nucleolar ribonucleoproteins (snoRNPs), directs 2′-O-methylation of rRNA and is also involved in other aspects of rRNA processing. A gene trap...
Functional characterization of SR and SR-related genes in Caenorhabditis elegans
Longman, Dása, Johnstone, Iain L., Cáceres, Javier F.
The SR proteins constitute a family of nuclear phosphoproteins, which are required for constitutive splicing and also influence alternative splicing regulation. Initially, it was suggested that SR...
A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
Cáceres, Javier F., Screaton, Gavin R., Krainer, Adrian R.
The SR proteins constitute a large family of nuclear phosphoproteins required for constitutive pre-mRNA splicing. These factors also have global, concentration-dependent effects on alternative...
A novel role for shuttling SR proteins in mRNA translation
Sanford, Jeremy R., Gray, Nicola K., Beckmann, Karsten, Cáceres, Javier F.
The Ser-Arg-rich (SR) proteins comprise a large family of nuclear phosphoproteins that are required for constitutive and alternative splicing. A subset of SR proteins shuttles continuously between...
Allemand, Eric, Guil, Sònia, Myers, Michael, Moscat, Jorge, Cáceres, Javier F., Krainer, Adrian R.
Heterogenous nuclear ribonucleoprotein (hnRNP) A1 is an alternative splicing factor that is mainly nuclear, although it shuttles rapidly between nuclear and cytoplasmic compartments. Cells stressed...
A Novel SR-Related Protein Is Required for the Second Step of Pre-mRNA Splicing
Cazalla, Demian, Newton, Kathryn, Cáceres, Javier F.
The SR family proteins and SR-related polypeptides are important regulators of pre-mRNA splicing. A novel SR-related protein of an apparent molecular mass of 53 kDa was isolated in a gene trap screen...
Sanford, Jeremy R., Ellis, Jonathan D., Cazalla, Demian, Cáceres, Javier F.
The Ser/Arg-rich (SR) proteins constitute a family of highly conserved nuclear phosphoproteins that are involved in many steps of mRNA metabolism. Previously, we demonstrated that shuttling SR...
hnRNP A1 Relocalization to the Stress Granules Reflects a Role in the Stress Response
Guil, Sonia, Long, Jennifer C., Cáceres, Javier F.
hnRNP A1 is a nucleocytoplasmic shuttling protein that is involved in many aspects of mRNA metabolism. We have previously shown that activation of the p38 stress-signaling pathway in mammalian cells...
Allemand, Eric, Gattoni, Renata, Bourbon, Henri-Marc, Stevenin, James, Cáceres, Javier F., Soret, Johann, ...
The human splicing factor 2, also called human alternative splicing factor (hASF), is the prototype of the highly conserved SR protein family involved in constitutive and regulated splicing of...
Eperon, Ian C., Makarova, Olga V., Mayeda, Akila, Munroe, Stephen H., Cáceres, Javier F., Hayward, Daniel G., ...
The first component known to recognize and discriminate among potential 5′ splice sites (5′SSs) in pre-mRNA is the U1 snRNP. However, the relative levels of U1 snRNP binding to alternative...
Role of SR protein modular domains in alternative splicing specificity in vivo
Van Der Houven Van Oordt, Willemien, Newton, Kathryn, Screaton, Gavin R., Cáceres, Javier F.
The SR proteins constitute a family of nuclear phosphoproteins which are required for constitutive splicing and also influence alternative splicing regulation. They have a modular structure...
Nuclear Export and Retention Signals in the RS Domain of SR Proteins
Cazalla, Demian, Zhu, Jun, Manche, Lisa, Huber, Elisabeth, Krainer, Adrian R., Cáceres, Javier F.
Splicing factors of the SR protein family share a modular structure consisting of one or two RNA recognition motifs (RRMs) and a C-terminal RS domain rich in arginine and serine residues. The RS...
Newton, Kathryn, Petfalski, Elisabeth, Tollervey, David, Cáceres, Javier F.
Fibrillarin, a protein component of C/D box small nucleolar ribonucleoproteins (snoRNPs), directs 2′-O-methylation of rRNA and is also involved in other aspects of rRNA processing. A gene trap...
Functional characterization of SR and SR-related genes in Caenorhabditis elegans
Longman, Dása, Johnstone, Iain L., Cáceres, Javier F.
The SR proteins constitute a family of nuclear phosphoproteins, which are required for constitutive splicing and also influence alternative splicing regulation. Initially, it was suggested that SR...
A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
Cáceres, Javier F., Screaton, Gavin R., Krainer, Adrian R.
The SR proteins constitute a large family of nuclear phosphoproteins required for constitutive pre-mRNA splicing. These factors also have global, concentration-dependent effects on alternative...
A novel role for shuttling SR proteins in mRNA translation
Sanford, Jeremy R., Gray, Nicola K., Beckmann, Karsten, Cáceres, Javier F.
The Ser-Arg-rich (SR) proteins comprise a large family of nuclear phosphoproteins that are required for constitutive and alternative splicing. A subset of SR proteins shuttles continuously between...
Allemand, Eric, Guil, Sònia, Myers, Michael, Moscat, Jorge, Cáceres, Javier F., Krainer, Adrian R.
Heterogenous nuclear ribonucleoprotein (hnRNP) A1 is an alternative splicing factor that is mainly nuclear, although it shuttles rapidly between nuclear and cytoplasmic compartments. Cells stressed...
A Novel SR-Related Protein Is Required for the Second Step of Pre-mRNA Splicing
Cazalla, Demian, Newton, Kathryn, Cáceres, Javier F.
The SR family proteins and SR-related polypeptides are important regulators of pre-mRNA splicing. A novel SR-related protein of an apparent molecular mass of 53 kDa was isolated in a gene trap screen...
Sanford, Jeremy R., Ellis, Jonathan D., Cazalla, Demian, Cáceres, Javier F.
The Ser/Arg-rich (SR) proteins constitute a family of highly conserved nuclear phosphoproteins that are involved in many steps of mRNA metabolism. Previously, we demonstrated that shuttling SR...
hnRNP A1 Relocalization to the Stress Granules Reflects a Role in the Stress Response
Guil, Sonia, Long, Jennifer C., Cáceres, Javier F.
hnRNP A1 is a nucleocytoplasmic shuttling protein that is involved in many aspects of mRNA metabolism. We have previously shown that activation of the p38 stress-signaling pathway in mammalian cells...
Mechanistic insights and identification of two novel factors in the C. elegans NMD pathway
Longman, Dasa, Plasterk, Ronald H.A., Johnstone, Iain L., Cáceres, Javier F.
The nonsense-mediated mRNA decay (NMD) pathway selectively degrades mRNAs harboring premature termination codons (PTCs). Seven genes (smg-1–7, for suppressor with morphological effect on genitalia)...
Misteli, Tom, Cáceres, Javier F., Clement, Jade Q., Krainer, Adrian R., Wilkinson, Miles F., Spector, David L.
Expression of most RNA polymerase II transcripts requires the coordinated execution of transcription, splicing, and 3′ processing. We have previously shown that upon transcriptional activation of a...
Cáceres, Javier F., Misteli, Tom, Screaton, Gavin R., Spector, David L., Krainer, Adrian R.
SR proteins are required for constitutive pre-mRNA splicing and also regulate alternative splice site selection in a concentration-dependent manner. They have a modular structure that consists of one...
Van Der Houven Van Oordt, Willemien, Diaz-Meco, María T., Lozano, José, Krainer, Adrian R., Moscat, Jorge, Cáceres, Javier F.
Individual members of the serine-arginine (SR) and heterogeneous nuclear ribonucleoprotein (hnRNP) A/B families of proteins have antagonistic effects in regulating alternative splicing. Although...