Myosin Va Bound to Phagosomes Binds to F-Actin and Delays Microtubule-dependent Motility
Al-Haddad, Ahmed, Shonn, Marion A., Redlich, Bärbel, Blocker, Ariel, Burkhardt, Janis K., Yu, Hanry, ...
We established a light microscopy-based assay that reconstitutes the binding of phagosomes purified from mouse macrophages to preassembled F-actin in vitro. Both endogenous myosin Va from mouse...
Molecular Ordering of the Initial Signaling Events of CD95
Algeciras-Schimnich, Alicia, Shen, Le, Barnhart, Bryan C., Murmann, Andrea E., Burkhardt, Janis K., Peter, Marcus E.
Binding of either ligand or agonistic antibodies to the death receptor CD95 (APO-1/Fas) induces the formation of the death-inducing signaling complex (DISC). We now show that signal initiation of...
Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport
Zhao, Huiren, Shiue, Harn, Palkon, Sara, Wang, Yingmin, Cullinan, Patrick, Burkhardt, Janis K., ...
Initiation of Na+-glucose cotransport in intestinal epithelial cells leads to activation of the apical Na+-H+ exchanger NHE3 and subsequent increases in cytoplasmic pH (pHi). This process requires...
Myosin Va Bound to Phagosomes Binds to F-Actin and Delays Microtubule-dependent Motility
Al-Haddad, Ahmed, Shonn, Marion A., Redlich, Bärbel, Blocker, Ariel, Burkhardt, Janis K., Yu, Hanry, ...
We established a light microscopy-based assay that reconstitutes the binding of phagosomes purified from mouse macrophages to preassembled F-actin in vitro. Both endogenous myosin Va from mouse...
Molecular Ordering of the Initial Signaling Events of CD95
Algeciras-Schimnich, Alicia, Shen, Le, Barnhart, Bryan C., Murmann, Andrea E., Burkhardt, Janis K., Peter, Marcus E.
Binding of either ligand or agonistic antibodies to the death receptor CD95 (APO-1/Fas) induces the formation of the death-inducing signaling complex (DISC). We now show that signal initiation of...
Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport
Zhao, Huiren, Shiue, Harn, Palkon, Sara, Wang, Yingmin, Cullinan, Patrick, Burkhardt, Janis K., ...
Initiation of Na+-glucose cotransport in intestinal epithelial cells leads to activation of the apical Na+-H+ exchanger NHE3 and subsequent increases in cytoplasmic pH (pHi). This process requires...
PI3K regulates pleckstrin-2 in T-cell cytoskeletal reorganization
Bach, Tami L., Kerr, Wesley T., Wang, Yanfeng, Bauman, Eve Marie, Kine, Purnima, Whiteman, Eileen L., ...
Pleckstrin-2 is composed of 2 pleckstrin homology (PH) domains and a disheveled–Egl-10–pleckstrin (DEP) domain. A lipid-binding assay revealed that pleckstrin-2 binds with greatest affinity to D3...
Tong, Jiankun, Allenspach, Eric J., Takahashi, Stephenie M., Mody, Purvi D., Park, Chan, Burkhardt, Janis K., ...
CD43 is a large heavily glycosylated protein highly expressed on T cells and actively excluded from the immunological synapse through interactions with ezrin-radixin-moesin proteins. Due to its size...
Burkhardt, Janis K., Echeverri, Christophe J., Nilsson, Tommy, Vallee, Richard B.
Dynactin is a multisubunit complex that plays an accessory role in cytoplasmic dynein function. Overexpression in mammalian cells of one dynactin subunit, dynamitin, disrupts the complex, resulting...
Molecular Requirements for Bi-directional Movement of Phagosomes Along Microtubules
Blocker, Ariel, Severin, Fedor F., Burkhardt, Janis K., Bingham, James B., Yu, Hanry, Olivo, Jean-Christophe, ...
Microtubules facilitate the maturation of phagosomes by favoring their interactions with endocytic compartments. Here, we show that phagosomes move within cells along tracks of several microns...