Minimizing the risk of reporting false positives in large-scale RNAi screens (2006)
Echeverri, Christophe J, Beachy, Philip A, Baum, Buzz, Boutros, Michael, Buchholz, Frank, Chanda, Sumit K, ...
Large-scale RNA interference (RNAi)-based analyses, very much as other 'omic' approaches, have inherent rates of false positives and negatives. The variability in the standards of care applied to...
[Mikrofiche-Ausg.].
A New Model for Nuclear Envelope BreakdownV⃞
Terasaki, Mark, Campagnola, Paul, Rolls, Melissa M., Stein, Pascal A., Ellenberg, Jan, Hinkle, Beth, ...
Nuclear envelope breakdown was investigated during meiotic maturation of starfish oocytes. Fluorescent 70-kDa dextran entry, as monitored by confocal microscopy, consists of two phases, a slow...
A Bromodomain Protein, MCAP, Associates with Mitotic Chromosomes and Affects G2-to-M Transition
Dey, Anup, Ellenberg, Jan, Farina, Andrea, Coleman, Allen E., Maruyama, Tetsuo, Sciortino, Selvaggia, ...
We describe a novel nuclear factor called mitotic chromosome-associated protein (MCAP), which belongs to the poorly understood BET subgroup of the bromodomain superfamily. Expression of the 200-kDa...
Forler, Daniel, Rabut, Gwénaël, Ciccarelli, Francesca D., Herold, Andrea, Köcher, Thomas, Niggeweg, Ricarda, ...
Metazoan NXF1-p15 heterodimers promote the nuclear export of bulk mRNA across nuclear pore complexes (NPCs). In vitro, NXF1-p15 forms a stable complex with the nucleoporin RanBP2/Nup358, a component...
Automatic Identification of Subcellular Phenotypes on Human Cell Arrays
Conrad, Christian, Erfle, Holger, Warnat, Patrick, Daigle, Nathalie, Lörch, Thomas, Ellenberg, Jan, ...
Light microscopic analysis of cell morphology provides a high-content readout of cell function and protein localization. Cell arrays and microwell transfection assays on cultured cells have made cell...
Loïodice, Isabelle, Alves, Annabelle, Rabut, Gwénaël, Van Overbeek, Megan, Ellenberg, Jan, Sibarita, Jean-Baptiste, ...
In eukaryotes, bidirectional transport of macromolecules between the cytoplasm and the nucleus occurs through elaborate supramolecular structures embedded in the nuclear envelope, the nuclear pore...
CHUSAINOW, JANET, AJUH, PAUL M., TRINKLE-MULCAHY, LAURA, SLEEMAN, JUDITH E., ELLENBERG, JAN, LAMOND, ANGUS I.
We have analyzed the interaction between the U2AF subunits U2AF35 and U2AF65 in vivo using fluorescence resonance energy transfer (FRET) microscopy. U2 snRNP Auxiliary Factor (U2AF) is an essential...
NuSAP, a Mitotic RanGTP Target That Stabilizes and Cross-links Microtubules
Ribbeck, Katharina, Groen, Aaron C., Santarella, Rachel, Bohnsack, Markus T., Raemaekers, Tim, Köcher, Thomas, ...
Nucleolar and spindle-associated protein (NuSAP) was recently identified as a microtubule- and chromatin-binding protein in vertebrates that is nuclear during interphase. Small interfering...
A New Model for Nuclear Envelope BreakdownV⃞
Terasaki, Mark, Campagnola, Paul, Rolls, Melissa M., Stein, Pascal A., Ellenberg, Jan, Hinkle, Beth, ...
Nuclear envelope breakdown was investigated during meiotic maturation of starfish oocytes. Fluorescent 70-kDa dextran entry, as monitored by confocal microscopy, consists of two phases, a slow...
A Bromodomain Protein, MCAP, Associates with Mitotic Chromosomes and Affects G2-to-M Transition
Dey, Anup, Ellenberg, Jan, Farina, Andrea, Coleman, Allen E., Maruyama, Tetsuo, Sciortino, Selvaggia, ...
We describe a novel nuclear factor called mitotic chromosome-associated protein (MCAP), which belongs to the poorly understood BET subgroup of the bromodomain superfamily. Expression of the 200-kDa...
Forler, Daniel, Rabut, Gwénaël, Ciccarelli, Francesca D., Herold, Andrea, Köcher, Thomas, Niggeweg, Ricarda, ...
Metazoan NXF1-p15 heterodimers promote the nuclear export of bulk mRNA across nuclear pore complexes (NPCs). In vitro, NXF1-p15 forms a stable complex with the nucleoporin RanBP2/Nup358, a component...
Automatic Identification of Subcellular Phenotypes on Human Cell Arrays
Conrad, Christian, Erfle, Holger, Warnat, Patrick, Daigle, Nathalie, Lörch, Thomas, Ellenberg, Jan, ...
Light microscopic analysis of cell morphology provides a high-content readout of cell function and protein localization. Cell arrays and microwell transfection assays on cultured cells have made cell...
Loïodice, Isabelle, Alves, Annabelle, Rabut, Gwénaël, Van Overbeek, Megan, Ellenberg, Jan, Sibarita, Jean-Baptiste, ...
In eukaryotes, bidirectional transport of macromolecules between the cytoplasm and the nucleus occurs through elaborate supramolecular structures embedded in the nuclear envelope, the nuclear pore...
CHUSAINOW, JANET, AJUH, PAUL M., TRINKLE-MULCAHY, LAURA, SLEEMAN, JUDITH E., ELLENBERG, JAN, LAMOND, ANGUS I.
We have analyzed the interaction between the U2AF subunits U2AF35 and U2AF65 in vivo using fluorescence resonance energy transfer (FRET) microscopy. U2 snRNP Auxiliary Factor (U2AF) is an essential...
NuSAP, a Mitotic RanGTP Target That Stabilizes and Cross-links Microtubules
Ribbeck, Katharina, Groen, Aaron C., Santarella, Rachel, Bohnsack, Markus T., Raemaekers, Tim, Köcher, Thomas, ...
Nucleolar and spindle-associated protein (NuSAP) was recently identified as a microtubule- and chromatin-binding protein in vertebrates that is nuclear during interphase. Small interfering...
Dissecting the Contribution of Diffusion and Interactions to the Mobility of Nuclear Proteins
Beaudouin, Joël, Mora-Bermúdez, Felipe, Klee, Thorsten, Daigle, Nathalie, Ellenberg, Jan
Quantitative characterization of protein interactions under physiological conditions is vital for systems biology. Fluorescence photobleaching/activation experiments of GFP-tagged proteins are...
Leung, Anthony Kar Lun, Gerlich, Daniel, Miller, Gail, Lyon, Carol, Lam, Yun Wah, Lleres, David, ...
One of the great mysteries of the nucleolus surrounds its disappearance during mitosis and subsequent reassembly at late mitosis. Here, the relative dynamics of nucleolar disassembly and reformation...
Bubulya, Paula A., Prasanth, Kannanganattu V., Deerinck, Thomas J., Gerlich, Daniel, Beaudouin, Joel, Ellisman, Mark H., ...
Upon completion of mitosis, daughter nuclei assemble all of the organelles necessary for the implementation of nuclear functions. We found that upon entry into daughter nuclei, snRNPs and SR proteins...
Ellenberg, Jan, Siggia, Eric D., Moreira, Jorge E., Smith, Carolyn L., Presley, John F., Worman, Howard J., ...
The mechanisms of localization and retention of membrane proteins in the inner nuclear membrane and the fate of this membrane system during mitosis were studied in living cells using the inner...
Retrograde Transport of Golgi-localized Proteins to the ER
Cole, Nelson B., Ellenberg, Jan, Song, Jia, DiEuliis, Diane, Lippincott-Schwartz, Jennifer
The ER is uniquely enriched in chaperones and folding enzymes that facilitate folding and unfolding reactions and ensure that only correctly folded and assembled proteins leave this compartment. Here...
Hirschberg, Koret, Miller, Chad M., Ellenberg, Jan, Presley, John F., Siggia, Eric D., Phair, Robert D., ...
Quantitative time-lapse imaging data of single cells expressing the transmembrane protein, vesicular stomatitis virus ts045 G protein fused to green fluorescent protein (VSVG–GFP), were used for...
Sloan-Lancaster, Joanne, Presley, John, Ellenberg, Jan, Yamazaki, Tetsuo, Lippincott-Schwartz, Jennifer, Samelson, Lawrence E.
The nonreceptor protein tyrosine kinase ZAP-70 is a critical enzyme required for successful T lymphocyte activation. After antigenic stimulation, ZAP-70 rapidly associates with T cell receptor (TCR)...
Belgareh, Naïma, Rabut, Gwénaël, Baï, Siau Wei, Van Overbeek, Megan, Beaudouin, Joël, Daigle, Nathalie, ...
The nuclear pore complexes (NPCs) are evolutionarily conserved assemblies that allow traffic between the cytoplasm and the nucleus. In this study, we have identified and characterized a novel human...
Lénárt, Péter, Rabut, Gwénaël, Daigle, Nathalie, Hand, Arthur R., Terasaki, Mark, Ellenberg, Jan
Breakdown of the nuclear envelope (NE) was analyzed in live starfish oocytes using a size series of fluorescently labeled dextrans, membrane dyes, and GFP-tagged proteins of the nuclear pore complex...
NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization
Raemaekers, Tim, Ribbeck, Katharina, Beaudouin, Joël, Annaert, Wim, Van Camp, Mark, Stockmans, Ingrid, ...
Here, we report on the identification of nucleolar spindle–associated protein (NuSAP), a novel 55-kD vertebrate protein with selective expression in proliferating cells. Its mRNA and protein levels...
Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p
Kruse, Claudia, Jaedicke, Andreas, Beaudouin, Joël, Böhl, Florian, Ferring, Dunja, Güttler, Thomas, ...
Class V myosins are motor proteins with functions in vesicle transport, organelle segregation, and RNA localization. Although they have been extensively studied, only little is known about the...
Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells
Daigle, Nathalie, Beaudouin, Joël, Hartnell, Lisa, Imreh, Gabriela, Hallberg, Einar, Lippincott-Schwartz, Jennifer, ...
The nuclear pore complex (NPC) and its relationship to the nuclear envelope (NE) was characterized in living cells using POM121–green fluorescent protein (GFP) and GFP-Nup153, and GFP–lamin B1....
Systematic kinetic analysis of mitotic dis- and reassembly of the nuclear pore in living cells
Dultz, Elisa, Zanin, Esther, Wurzenberger, Claudia, Braun, Marion, Rabut, Gwénaël, Sironi, Lucia, ...
During mitosis in higher eukaryotes, nuclear pore complexes (NPCs) disassemble in prophase and are rebuilt in anaphase and telophase. NPC formation is hypothesized to occur by the interaction of...