Scientific Commons: James V. Anderson

Signals from both leaves and apical or axillary meristems of leafy spurge are known to inhibit root bud growth. To test the hypothesis that carbohydrates and growth regulators affect root bud growth,...

Transcriptome analysis of paradormancy release in root buds of leafy spurge (Euphorbia esula) (2005)

David P. Horvath, Mauricio Soto-Suárez, Wun S. Chao, Ying Jia, James V. Anderson

Bud dormancy is the primary mechanism by which the many perennial weeds escape herbicidal and mechanical control. We developed a 2,654-element Euphorbiaceae cDNA microarray using 1,886 sequenced...

Potential model weeds to study genomics, ecology, and physiology in the 21st century (2005)

Wun S. Chao, Dave P. Horvath, James V. Anderson, Michael E. Foley

Plant model systems have contributed greatly to the dramatic progress in understanding the fundamental aspects of plant biology. Using model weeds will also help facilitate focused funding and...

Cloning, characterization, and expression of growth regulator CYCLIN D3-2 in leafy spurge (Euphorbia esula) (2005)

David P. Horvath, James V. Anderson, Ying Jia, Wun S. Chao

We have isolated both a genomic and near full length cDNA clone for a D-class cyclin gene from the perennial weed leafy spurge. Sequence analysis indicates that this gene has the highest similarity...

A molecular approach to understanding root bud dormancy in leafy spurge (2002)

David P. Horvath, James V. Anderson

Leafy spurge is a tenacious perennial weed of the Northern Plains. This plant maintains a perennial growth cycle by controlled production and growth of numerous underground adventitious buds. We are...

Review: A current review on the regulation of dormancy in vegetative buds (2001)

James V. Anderson, Wun S. Chao, David P. Horvath

In this review, we examine current techniques and recent advances directed toward understanding cellular mechanisms involved in controlling dormancy in vegetative propagules. Vegetative propagules...

Random sequencing of cDNAs and identification of mRNAs (2001)

James V. Anderson, David P. Horvath

As a first step toward developing a genomics-based research program to study growth and development of underground adventitious shoot buds of leafy spurge, we initiated a leafy spurge expressed...

Molecular Analysis of Signals Controlling Dormancy and Growth in Underground Adventitious Buds of Leafy Spurge

Horvath, David P., Chao, Wun S., Anderson, James V.

Dormancy and subsequent regrowth of adventitious buds is a critical physiological process for many perennial plants. We have used the expression of hormone and cell cycle-responsive genes as markers...

Purification of Multiple Forms of Glutathione Reductase from Pea (Pisum sativum L.) Seedlings and Enzyme Levels in Ozone-Fumigated Pea Leaves 1

Madamanchi, Nageswara R., Anderson, James V., Alscher, Ruth G., Cramer, Carole L., Hess, John L.

Glutathione reductase was purified from pea seedlings using a procedure that included 2′,5′-ADP Sepharose, fast protein liquid chromatography (FPLC)-anion exchange, and FPLC-hydrophobic...

Purification, Characterization, and Immunological Properties for Two Isoforms of Glutathione Reductase from Eastern White Pine Needles 1

Anderson, James V., Hess, John L., Chevone, Boris I.

Glutathione reductase (EC 1.6.4.2) was purified from Eastern white pine (Pinus strobus L.) needles. The purification steps included affinity chromatography using 2′, 5′-ADP-Sepharose,...

Seasonal Variation in the Antioxidant System of Eastern White Pine Needles 1: Evidence for Thermal Dependence

Anderson, James V., Chevone, Boris I., Hess, John L.

Antioxidant metabolites in eastern white pine (Pinus strobus L.) needles increased two- to fourfold from the summer to the winter season. Antioxidant enzymes in needle tissue increased between 2- and...