Analysis of Genetic Interaction Maps Reveals Functional Pleiotropy (2008)
Shuye Pu, Karen Ronen, James Vlasblom, Jack Greenblatt, Shoshana J. Wodak
Epistatic or genetic interactions, representing the effects of mutations on the phenotypes caused by other mutations, can be very helpful for uncovering functional relationships between genes....
Pitre, Sylvain, Dehne, Frank, Chan, Albert, Cheetham, Jim, Duong, Alex, Emili, Andrew, ...
Abstract Background Identification of protein interaction networks has received considerable attention in the post-genomic era. The currently available biochemical approaches used to detect...
The functional landscape of mouse gene expression (2004)
Zhang, Wen, Morris, Quaid D, Chang, Richard, Shai, Ofer, Bakowski, Malina A, Mitsakakis, Nicholas, ...
Abstract Background Large-scale quantitative analysis of transcriptional co-expression has been used to dissect regulatory networks and to predict the functions of new genes discovered by genome...
A cofactor, TIP30, specifically enhances HIV-1 Tatactivated transcription
Xiao, Hua, Tao, Yong, Greenblatt, Jack, Roeder, Robert G.
Replication of HIV-1 requires the viral Tat protein, which increases the extent of transcription elongation by RNA polymerase II after activation at the single viral long terminal repeat (LTR)...
Forget, Diane, Robert, François, Grondin, Gilles, Burton, Zachary F., Greenblatt, Jack, Coulombe, Benoit
RAP74, the large subunit of transcription factor IIF, associates with a preinitiation complex containing RNA polymerase II (pol II) and other general initiation factors. We have mapped the location...
Archambault, Jacques, Chambers, Ross S., Kobor, Michael S., Ho, Yuen, Cartier, Mireille, Bolotin, Diana, ...
One of the essential components of a phosphatase that specifically dephosphorylates the Saccharomyces cerevisiae RNA polymerase II (RPII) large subunit C-terminal domain (CTD) is a novel polypeptide...
Kobor, Michael S., Simon, Lisa D., Omichinski, Jim, Zhong, Guoqing, Archambault, Jacques, Greenblatt, Jack
Transcription by RNA polymerase II is accompanied by cyclic phosphorylation and dephosphorylation of the carboxy-terminal heptapeptide repeat domain (CTD) of its largest subunit. We have used...
Specific Binding of Escherichia coli Ribosomal Protein S1 to boxA Transcriptional Antiterminator RNA
Mogridge, Jeremy, Greenblatt, Jack
We show that ribosomal protein S1 specifically binds the boxA transcriptional antiterminator RNAs of bacteriophage λ and the Escherichia coli ribosomal RNA operons. Although S1 competes with the...
Stimulation of Transcription by Mutations Affecting Conserved Regions of RNA Polymerase II
Archambault, Jacques, Jansma, David B., Kawasoe, Jean H., Arndt, Kim T., Greenblatt, Jack, Friesen, James D.
Mutations that increase the low-level transcription of the Saccharomyces cerevisiae HIS4 gene, which results from deletion of the genes encoding transcription factors BAS1, BAS2, and GCN4, were...
Nguyen, Bao D., Abbott, Karen L., Potempa, Krzysztof, Kobor, Michael S., Archambault, Jacques, Greenblatt, Jack, ...
FCP1 [transcription factor IIF (TFIIF)-associated carboxyl-terminal domain (CTD) phosphatase] is the only identified phosphatase specific for the phosphorylated CTD of RNA polymerase II (RNAP II)....
The α subunit of E. coli RNA polymerase activates RNA binding by NusA
Mah, Thien-Fah, Kuznedelov, Konstantin, Mushegian, Arcady, Severinov, Konstantin, Greenblatt, Jack
The Escherichia coli NusA protein modulates pausing, termination, and antitermination by associating with the transcribing RNA polymerase core enzyme. NusA can be covalently cross-linked to nascent...
Baetz, Kristin K., Krogan, Nevan J., Emili, Andrew, Greenblatt, Jack, Hieter, Philip
The budding yeast centromere-kinetochore complex ensures high-fidelity chromosome segregation in mitosis and meiosis by mediating the attachment and movement of chromosomes along spindle...
Positive Control of Endolysin Synthesis In Vitro by the Gene N Protein of Phage λ
Positive control in vitro by gene N protein of bacteriophage λ was demonstrated. λ DNA was used to direct in vitro synthesis of λ endolysin in a cell-free protein-synthesizing preparation derived...
Bromodomain Factor 1 (Bdf1) Is Phosphorylated by Protein Kinase CK2
Sawa, Chika, Nedea, Eduard, Krogan, Nevan, Wada, Tadashi, Handa, Hiroshi, Greenblatt, Jack, ...
Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is...
Krogan, Nevan J., Kim, Minkyu, Tong, Amy, Golshani, Ashkan, Cagney, Gerard, Canadien, Veronica, ...
Set2 methylates Lys36 of histone H3. We show here that yeast Set2 copurifies with RNA polymerase II (RNAPII). Chromatin immunoprecipitation analyses demonstrated that Set2 and histone H3 Lys36...
Regulation of the Expression of the N Gene of Bacteriophage Lambda
A quantitative assay for the N protein of bacteriophage λ has been used to study the in vivo regulation of N gene expression. The assay makes use of the observation that in a cell-free...
Jeronimo, Célia, Langelier, Marie-France, Zeghouf, Mahel, Cojocaru, Marilena, Bergeron, Dominique, Baali, Dania, ...
We have programmed human cells to express physiological levels of recombinant RNA polymerase II (RNAPII) subunits carrying tandem affinity purification (TAP) tags. Double-affinity chromatography...
The functional landscape of mouse gene expression
Zhang, Wen, Morris, Quaid D, Chang, Richard, Shai, Ofer, Bakowski, Malina A, Mitsakakis, Nicholas, ...
Langelier, Marie-France, Baali, Dania, Trinh, Vincent, Greenblatt, Jack, Archambault, Jacques, Coulombe, Benoit
During transcription by RNA polymerase (RNAP) II, the incoming ribonucleoside triphosphate (NTP) enters the catalytic center in association with an Mg2+ ion, termed metal B [Mg(B)]. When bound to...
Esf2p, a U3-Associated Factor Required for Small-Subunit Processome Assembly and Compaction
Hoang, Tran, Peng, Wen-Tao, Vanrobays, Emmanuel, Krogan, Nevan, Hiley, Shawna, Beyer, Ann L., ...
Esf2p is the Saccharomyces cerevisiae homolog of mouse ABT1, a protein previously identified as a putative partner of the TATA-element binding protein. However, large-scale studies have indicated...
Cho, Eun-Jung, Kobor, Michael S., Kim, Minkyu, Greenblatt, Jack, Buratowski, Stephen
The C-terminal domain (CTD) of the RNA polymerase II (Pol II) largest subunit is hyperphosphorylated during transcription. Using an in vivo cross-linking/chromatin immunoprecipitation assay, we found...
A cofactor, TIP30, specifically enhances HIV-1 Tatactivated transcription
Xiao, Hua, Tao, Yong, Greenblatt, Jack, Roeder, Robert G.
Replication of HIV-1 requires the viral Tat protein, which increases the extent of transcription elongation by RNA polymerase II after activation at the single viral long terminal repeat (LTR)...
Forget, Diane, Robert, François, Grondin, Gilles, Burton, Zachary F., Greenblatt, Jack, Coulombe, Benoit
RAP74, the large subunit of transcription factor IIF, associates with a preinitiation complex containing RNA polymerase II (pol II) and other general initiation factors. We have mapped the location...
Archambault, Jacques, Chambers, Ross S., Kobor, Michael S., Ho, Yuen, Cartier, Mireille, Bolotin, Diana, ...
One of the essential components of a phosphatase that specifically dephosphorylates the Saccharomyces cerevisiae RNA polymerase II (RPII) large subunit C-terminal domain (CTD) is a novel polypeptide...
Kobor, Michael S., Simon, Lisa D., Omichinski, Jim, Zhong, Guoqing, Archambault, Jacques, Greenblatt, Jack
Transcription by RNA polymerase II is accompanied by cyclic phosphorylation and dephosphorylation of the carboxy-terminal heptapeptide repeat domain (CTD) of its largest subunit. We have used...
Specific Binding of Escherichia coli Ribosomal Protein S1 to boxA Transcriptional Antiterminator RNA
Mogridge, Jeremy, Greenblatt, Jack
We show that ribosomal protein S1 specifically binds the boxA transcriptional antiterminator RNAs of bacteriophage λ and the Escherichia coli ribosomal RNA operons. Although S1 competes with the...
Stimulation of Transcription by Mutations Affecting Conserved Regions of RNA Polymerase II
Archambault, Jacques, Jansma, David B., Kawasoe, Jean H., Arndt, Kim T., Greenblatt, Jack, Friesen, James D.
Mutations that increase the low-level transcription of the Saccharomyces cerevisiae HIS4 gene, which results from deletion of the genes encoding transcription factors BAS1, BAS2, and GCN4, were...
Nguyen, Bao D., Abbott, Karen L., Potempa, Krzysztof, Kobor, Michael S., Archambault, Jacques, Greenblatt, Jack, ...
FCP1 [transcription factor IIF (TFIIF)-associated carboxyl-terminal domain (CTD) phosphatase] is the only identified phosphatase specific for the phosphorylated CTD of RNA polymerase II (RNAP II)....
Cho, Eun-Jung, Kobor, Michael S., Kim, Minkyu, Greenblatt, Jack, Buratowski, Stephen
The C-terminal domain (CTD) of the RNA polymerase II (Pol II) largest subunit is hyperphosphorylated during transcription. Using an in vivo cross-linking/chromatin immunoprecipitation assay, we found...
The α subunit of E. coli RNA polymerase activates RNA binding by NusA
Mah, Thien-Fah, Kuznedelov, Konstantin, Mushegian, Arcady, Severinov, Konstantin, Greenblatt, Jack
The Escherichia coli NusA protein modulates pausing, termination, and antitermination by associating with the transcribing RNA polymerase core enzyme. NusA can be covalently cross-linked to nascent...
Baetz, Kristin K., Krogan, Nevan J., Emili, Andrew, Greenblatt, Jack, Hieter, Philip
The budding yeast centromere-kinetochore complex ensures high-fidelity chromosome segregation in mitosis and meiosis by mediating the attachment and movement of chromosomes along spindle...
Positive Control of Endolysin Synthesis In Vitro by the Gene N Protein of Phage λ
Positive control in vitro by gene N protein of bacteriophage λ was demonstrated. λ DNA was used to direct in vitro synthesis of λ endolysin in a cell-free protein-synthesizing preparation derived...
Bromodomain Factor 1 (Bdf1) Is Phosphorylated by Protein Kinase CK2
Sawa, Chika, Nedea, Eduard, Krogan, Nevan, Wada, Tadashi, Handa, Hiroshi, Greenblatt, Jack, ...
Bromodomain factor 1 (Bdf1) associates with Saccharomyces cerevisiae TFIID and corresponds to the C-terminal half of higher eukaryotic TAF1. It also associates with the SWR-C complex, which is...
Krogan, Nevan J., Kim, Minkyu, Tong, Amy, Golshani, Ashkan, Cagney, Gerard, Canadien, Veronica, ...
Set2 methylates Lys36 of histone H3. We show here that yeast Set2 copurifies with RNA polymerase II (RNAPII). Chromatin immunoprecipitation analyses demonstrated that Set2 and histone H3 Lys36...
Regulation of the Expression of the N Gene of Bacteriophage Lambda
A quantitative assay for the N protein of bacteriophage λ has been used to study the in vivo regulation of N gene expression. The assay makes use of the observation that in a cell-free...
Jeronimo, Célia, Langelier, Marie-France, Zeghouf, Mahel, Cojocaru, Marilena, Bergeron, Dominique, Baali, Dania, ...
We have programmed human cells to express physiological levels of recombinant RNA polymerase II (RNAPII) subunits carrying tandem affinity purification (TAP) tags. Double-affinity chromatography...
The functional landscape of mouse gene expression
Zhang, Wen, Morris, Quaid D, Chang, Richard, Shai, Ofer, Bakowski, Malina A, Mitsakakis, Nicholas, ...
Langelier, Marie-France, Baali, Dania, Trinh, Vincent, Greenblatt, Jack, Archambault, Jacques, Coulombe, Benoit
During transcription by RNA polymerase (RNAP) II, the incoming ribonucleoside triphosphate (NTP) enters the catalytic center in association with an Mg2+ ion, termed metal B [Mg(B)]. When bound to...
Esf2p, a U3-Associated Factor Required for Small-Subunit Processome Assembly and Compaction
Hoang, Tran, Peng, Wen-Tao, Vanrobays, Emmanuel, Krogan, Nevan, Hiley, Shawna, Beyer, Ann L., ...
Esf2p is the Saccharomyces cerevisiae homolog of mouse ABT1, a protein previously identified as a putative partner of the TATA-element binding protein. However, large-scale studies have indicated...
McGarvey, Tim, Rosonina, Emanuel, McCracken, Susan, Li, Qiyu, Arnaout, Ramy, Mientjes, Edwin, ...
DEK is an ∼45-kD phosphoprotein that is fused to the nucleoporin CAN as a result of a (6;9) chromosomal translocation in a subset of acute myeloid leukemias (AMLs). It has also been identified as...