Suter, Bernhard, Pogoutse, Oxana, Guo, Xinghua, Krogan, Nevan, Lewis, Peter, Greenblatt, Jack F, ...
Abstract Background Histone modifications have been implicated in the regulation of transcription and, more recently, in DNA replication and repair. In yeast, a major conserved histone...
COMPASS: A complex of proteins associated with a trithorax-related SET domain protein
Miller, Trissa, Krogan, Nevan J., Dover, Jim, Erdjument-Bromage, H., Tempst, Paul, Johnston, Mark, ...
The trithorax genes encode an evolutionarily conserved family of proteins that function to maintain specific patterns of gene expression throughout cellular development. Members of this protein...
Krogan, Nevan J., Greenblatt, Jack F.
The Elongator complex associated with elongating RNA polymerase II in Saccharomyces cerevisiae was originally reported to have three subunits, Elp1, Elp2, and Elp3. Using the tandem affinity...
RNA Polymerase II Elongation Factors of Saccharomyces cerevisiae: a Targeted Proteomics Approach
Krogan, Nevan J., Kim, Minkyu, Ahn, Seong Hoon, Zhong, Guoqing, Kobor, Michael S., Cagney, Gerard, ...
To physically characterize the web of interactions connecting the Saccharomyces cerevisiae proteins suspected to be RNA polymerase II (RNAPII) elongation factors, subunits of Spt4/Spt5 and Spt16/Pob3...
ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae
Peng, Wen-Tao, Krogan, Nevan J., Richards, Dawn P., Greenblatt, Jack F., Hughes, Timothy R.
We report that Esf1p (Ydr365cp), an essential, evolutionarily conserved nucleolar protein, is required for the biogenesis of 18S rRNA in Saccharomyces cerevisiae. Depletion of Esf1p resulted in...
Krogan, Nevan J., Baetz, Kristin, Keogh, Michael-Christopher, Datta, Nira, Sawa, Chika, Kwok, Trevor C. Y., ...
NuA4, the only essential histone acetyltransferase complex in Saccharomyces cerevisiae, acetylates the N-terminal tails of histones H4 and H2A. Affinity purification of NuA4 revealed the presence of...
Histone H2B Ubiquitylation Is Associated with Elongating RNA Polymerase II
Xiao, Tiaojiang, Kao, Cheng-Fu, Krogan, Nevan J., Sun, Zu-Wen, Greenblatt, Jack F., Osley, Mary Ann, ...
Rad6-mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3. However, how Rad6 and H2B...
Ingvarsdottir, Kristin, Krogan, Nevan J., Emre, N. C. Tolga, Wyce, Anastasia, Thompson, Natalie J., Emili, Andrew, ...
The SAGA complex is a multisubunit protein complex involved in transcriptional regulation in Saccharomyces cerevisiae. SAGA combines proteins involved in interactions with DNA-bound activators and...
Transitions in RNA polymerase II elongation complexes at the 3′ ends of genes
Kim, Minkyu, Ahn, Seong-Hoon, Krogan, Nevan J, Greenblatt, Jack F, Buratowski, Stephen
To understand the factor interactions of transcribing RNA polymerase II (RNApII) in vivo, chromatin immunoprecipitations were used to map the crosslinking patterns of multiple elongation and...
Prather, Donald, Krogan, Nevan J., Emili, Andrew, Greenblatt, Jack F., Winston, Fred
In order to identify previously unknown transcription elongation factors, a genetic screen was carried out to identify mutations that cause lethality when combined with mutations in the genes...
The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4
Keogh, Michael-Christopher, Mennella, Thomas A., Sawa, Chika, Berthelet, Sharon, Krogan, Nevan J., Wolek, Adam, ...
The histone H2A variant H2A.Z (Saccharomyces cerevisiae Htz1) plays roles in transcription, DNA repair, chromosome stability, and limiting telomeric silencing. The Swr1-Complex (SWR-C) inserts Htz1...
COMPASS: A complex of proteins associated with a trithorax-related SET domain protein
Miller, Trissa, Krogan, Nevan J., Dover, Jim, Erdjument-Bromage, H., Tempst, Paul, Johnston, Mark, ...
The trithorax genes encode an evolutionarily conserved family of proteins that function to maintain specific patterns of gene expression throughout cellular development. Members of this protein...
Krogan, Nevan J., Greenblatt, Jack F.
The Elongator complex associated with elongating RNA polymerase II in Saccharomyces cerevisiae was originally reported to have three subunits, Elp1, Elp2, and Elp3. Using the tandem affinity...
RNA Polymerase II Elongation Factors of Saccharomyces cerevisiae: a Targeted Proteomics Approach
Krogan, Nevan J., Kim, Minkyu, Ahn, Seong Hoon, Zhong, Guoqing, Kobor, Michael S., Cagney, Gerard, ...
To physically characterize the web of interactions connecting the Saccharomyces cerevisiae proteins suspected to be RNA polymerase II (RNAPII) elongation factors, subunits of Spt4/Spt5 and Spt16/Pob3...
ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae
Peng, Wen-Tao, Krogan, Nevan J., Richards, Dawn P., Greenblatt, Jack F., Hughes, Timothy R.
We report that Esf1p (Ydr365cp), an essential, evolutionarily conserved nucleolar protein, is required for the biogenesis of 18S rRNA in Saccharomyces cerevisiae. Depletion of Esf1p resulted in...
Krogan, Nevan J., Baetz, Kristin, Keogh, Michael-Christopher, Datta, Nira, Sawa, Chika, Kwok, Trevor C. Y., ...
NuA4, the only essential histone acetyltransferase complex in Saccharomyces cerevisiae, acetylates the N-terminal tails of histones H4 and H2A. Affinity purification of NuA4 revealed the presence of...
Histone H2B Ubiquitylation Is Associated with Elongating RNA Polymerase II
Xiao, Tiaojiang, Kao, Cheng-Fu, Krogan, Nevan J., Sun, Zu-Wen, Greenblatt, Jack F., Osley, Mary Ann, ...
Rad6-mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3. However, how Rad6 and H2B...
Ingvarsdottir, Kristin, Krogan, Nevan J., Emre, N. C. Tolga, Wyce, Anastasia, Thompson, Natalie J., Emili, Andrew, ...
The SAGA complex is a multisubunit protein complex involved in transcriptional regulation in Saccharomyces cerevisiae. SAGA combines proteins involved in interactions with DNA-bound activators and...
Transitions in RNA polymerase II elongation complexes at the 3′ ends of genes
Kim, Minkyu, Ahn, Seong-Hoon, Krogan, Nevan J, Greenblatt, Jack F, Buratowski, Stephen
To understand the factor interactions of transcribing RNA polymerase II (RNApII) in vivo, chromatin immunoprecipitations were used to map the crosslinking patterns of multiple elongation and...
Prather, Donald, Krogan, Nevan J., Emili, Andrew, Greenblatt, Jack F., Winston, Fred
In order to identify previously unknown transcription elongation factors, a genetic screen was carried out to identify mutations that cause lethality when combined with mutations in the genes...
The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4
Keogh, Michael-Christopher, Mennella, Thomas A., Sawa, Chika, Berthelet, Sharon, Krogan, Nevan J., Wolek, Adam, ...
The histone H2A variant H2A.Z (Saccharomyces cerevisiae Htz1) plays roles in transcription, DNA repair, chromosome stability, and limiting telomeric silencing. The Swr1-Complex (SWR-C) inserts Htz1...
Systems-level analyses identify extensive coupling among gene expression machines
Maciag, Karolina, Altschuler, Steven J, Slack, Michael D, Krogan, Nevan J, Emili, Andrew, Greenblatt, Jack F, ...
Here, we develop computational methods to assess and consolidate large, diverse protein interaction data sets, with the objective of identifying proteins involved in the coupling of multicomponent...
Xiao, Tiaojiang, Shibata, Yoichiro, Rao, Bhargavi, Laribee, R. Nicholas, O'Rourke, Rose, Buck, Michael J., ...
In yeast and other eukaryotes, the histone methyltransferase Set1 mediates methylation of lysine 4 on histone H3 (H3K4me). This modification marks the 5′ end of transcribed genes in a 5′-to-3′...
Suter, Bernhard, Pogoutse, Oxana, Guo, Xinghua, Krogan, Nevan, Lewis, Peter, Greenblatt, Jack F, ...
Suter, Bernhard, Fetchko, Michael J., Imhof, Ralph, Graham, Christopher I., Stoffel-Studer, Ingrid, Zbinden, Caroline, ...
Comprehensive approaches to detect protein–protein interactions (PPIs) have been most successful in the yeast model system. Here we present “Cross-and-Capture,” a novel assay for rapid,...
Chaperone Control of the Activity and Specificity of the Histone H3 Acetyltransferase Rtt109▿ †
Fillingham, Jeffrey, Recht, Judith, Silva, Andrea C., Suter, Bernhard, Emili, Andrew, Stagljar, Igor, ...
Acetylation of Saccharomyces cerevisiae histone H3 on K56 by the histone acetyltransferase (HAT) Rtt109 is important for repairing replication-associated lesions. Rtt109 purifies from yeast in...