Yosef Refaeli, Ryan M. Young, Brian C. Turner, Jennifer Duda, Kenneth A. Field, J. Michael Bishop
A variety of circumstantial evidence from humans has implicated the B cell antigen receptor (BCR) in the genesis of B cell lymphomas. We generated mouse models designed to test this possibility...
Field, Kenneth A, Charoenthongtrakul, Soratree, Bishop, J Michael, Refaeli, Yosef
Abstract Background We have used a mouse model based on overexpression of c-Myc in B cells genetically engineered to be self-reactive to test the hypothesis that farnesyl transferase inhibitors...
Padua, Rose Ann, Larghero, Jerome, Robin, Marie, Le Pogam, Carol, Schlageter, Marie-Helene, Muszlak, Sacha, ...
Despite improved molecular characterization of malignancies and development of targeted therapies, acute leukemia is not curable and few patients survive more than 10 years after diagnosis. Recently,...
Yang, Dun, Buchholz, Frank, Huang, Zhongdong, Goga, Andrei, Chen, Chih-Ying, Brodsky, Frances M., ...
Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing...
Hahn, Mounou, Bishop, J. Michael
The RET gene, encoding a receptor tyrosine kinase, is unusual among human protooncogenes in that its mutant alleles are implicated in a developmental defect involving enteric neurons as well as in...
A PMLRARα transgene initiates murine acute promyelocytic leukemia
Brown, Diane, Kogan, Scott, Lagasse, Eric, Weissman, Irving, Alcalay, Myriam, Pelicci, Pier Giuseppe, ...
The malignant cells of acute promyelocytic leukemia (APL) contain a reciprocal chromosomal translocation that fuses the promyelocytic leukemia gene (PML) with the retinoic acid receptor α gene...
Identification of a large Myc-binding protein that contains RCC1-like repeats
Guo, Qingbin, Xie, Jingwu, Dang, Chi V., Liu, Edison T., Bishop, J. Michael
The protooncogene MYC plays an important role in the regulation of cellular proliferation, differentiation, and apoptosis and has been implicated in a variety of human tumors. MYC and the closely...
Kogan, Scott C., Lagasse, Eric, Atwater, Susan, Bae, Suk-chul, Weissman, Irving, Ito, Yoshiaki, ...
Chromosomal translocations involving the genes encoding the alpha and beta subunits of the Pebp2/Cbf transcription factor have been associated with human acute myeloid leukemia and the preleukemic...
Smith-McCune, K., Kalman, D., Robbins, C., Shivakumar, S., Yuschenkoff, L., Bishop, J. Michael
To study intracellular pathways by which the human papillomavirus 16 oncogene E7 participates in carcinogenesis, we expressed an inducible chimera of E7 by fusion to the hormone-binding domain of the...
Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening
Zhu, Jiyue, Wang, He, Bishop, J. Michael, Blackburn, Elizabeth H.
Human fibroblasts whose lifespan in culture has been extended by expression of a viral oncogene eventually undergo a growth crisis marked by failure to proliferate. It has been proposed that telomere...
Transient excess of MYC activity can elicit genomic instability and tumorigenesis
Felsher, Dean W., Bishop, J. Michael
Overexpression of the MYC protooncogene has been implicated in the genesis of diverse human tumors. Tumorigenesis induced by MYC has been attributed to sustained effects on proliferation and...
Src interacts with dynamin and synapsin in neuronal cells
Foster-Barber, Audrey, Bishop, J. Michael
The nonreceptor tyrosine kinase Src is expressed at a high level in cells that are specialized for regulated secretion, such as the neuron, and is concentrated on secretory vesicles or at the site of...
Radany, Eric H., Hong, Karen, Kesharvarzi, Sima, Lander, Eric S., Bishop, J. Michael
Hybrid mice carrying oncogenic transgenes afford powerful systems for investigating loss of heterozygosity (LOH) in tumors. Here, we apply this approach to a neoplasm of key importance in human...
Overexpression of MYC causes p53-dependent G2 arrest of normal fibroblasts
Felsher, Dean W., Zetterberg, Anders, Zhu, Jiyue, Tlsty, Thea, Bishop, J. Michael
Overexpression of the proto-oncogene MYC has been implicated in the genesis of diverse human cancers. One explanation for the role of MYC in tumorigenesis has been that this gene might drive cells...
Ras Family GTPases Control Growth of Astrocyte Processes
Kalman, Daniel, Gomperts, Stephen N., Hardy, Stephen, Kitamura, Marina, Bishop, J. Michael
Astrocytes in neuron-free cultures typically lack processes, although they are highly process-bearing in vivo. We show that basic fibroblast growth factor (bFGF) induces cultured astrocytes to grow...
Cheng, Steven Yan, Bishop, J. Michael
The Suppressor of Fused [Su(fu)] protein plays a conserved role in the regulation of Gli transcription factors of the hedgehog (Hh) signaling pathway that controls cell fate and tissue patterning...
Yang, Dun, Buchholz, Frank, Huang, Zhongdong, Goga, Andrei, Chen, Chih-Ying, Brodsky, Frances M., ...
Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing...
Proposal for Naming Host Cell-Derived Inserts in Retrovirus Genomes †
Coffin, John M., Varmus, Harold E., Bishop, J. Michael, Essex, Myron, Hardy, William D., Martin, G. Steven, ...
We propose a system for naming inserted sequences in transforming retroviruses (i.e., onc genes), based on using trivial names derived from a prototype strain of virus.
Sheiness, Diana, Bister, Klaus, Moscovici, Carlo, Fanshier, Lois, Gonda, Thomas, Bishop, J. Michael
Avian myelocytomatosis virus (MC29V) is a retrovirus that transforms both fibroblasts and macrophages in culture and induces myelocytomatosis, carcinomas, and sarcomas in birds. Previous work...
Drosophila myb is required for the G2/M transition and maintenance of diploidy
Katzen, Alisa L., Jackson, Jean, Harmon, Brian P., Fung, Siau-Min, Ramsay, Gary, Bishop, J. Michael
The myb proto-oncogenes are thought to have a role in the cell division cycle. We have examined this possibility by genetic analysis in Drosophila melanogaster, which possesses a single myb gene. We...
Trumpp, Andreas, Depew, Michael J., Rubenstein, John L.R., Bishop, J. Michael, Martin, Gail R.
In mammals, the first branchial arch (BA1) develops into a number of craniofacial skeletal elements including the jaws and teeth. Outgrowth and patterning of BA1 during early embryogenesis is thought...
Senescence of human fibroblasts induced by oncogenic Raf
Zhu, Jiyue, Woods, Douglas, McMahon, Martin, Bishop, J. Michael
The oncogenes RAS and RAF came to view as agents of neoplastic transformation. However, in normal cells, these genes can have effects that run counter to oncogenic transformation, such as arrest of...
Negative Control of the Myc Protein by the Stress-Responsive Kinase Pak2
Huang, Zhongdong, Traugh, Jolinda A., Bishop, J. Michael
Pak2 is a serine/threonine kinase that participates in the cellular response to stress. Among the potential substrates for Pak2 is the protein Myc, encoded by the proto-oncogene MYC. Here we...
Sheiness, Diana, Bishop, J. Michael
The avian carcinoma virus MC29 (MC29V) contains a sequence of approximately 1,500 nucleotides which may represent a gene responsible for tumorigenesis by MC29V. We present evidence that MC29V has...
Varmus, Harold E., Heasley, Suzanne, Bishop, J. Michael
Labeled, virus-specific DNA synthesized in vitro by the virion-associated polymerase of avian sarcoma virus (ASV) was used to measure virus-specific sequences in cell DNA in three ways: (i) by...
Fanshier, Lois, Garapin, Axel-Claude, McDonnell, Jerome, Faras, Anthony, Levinson, Warren, Bishop, J. Michael
The products of the deoxyribonucleic acid (DNA) polymerase associated with Rous sarcoma virus and avian myeloblastosis virus were characterized by correlative analyses with equilibrium centrifugation...
Garapin, Axel-Claude, Fanshier, Lois, Leong, Jo-Ann, Jackson, Jean, Levinson, Warren, Bishop, J. Michael
The deoxyribonucleic acid (DNA) polymerase(s) of Rous sarcoma virus synthesizes two principal products—single-stranded DNA in the form of a DNA:ribonucleic acid (RNA) hybrid and double-stranded...
Faras, Anthony, Fanshier, Lois, Garapin, Axel-Claude, Levinson, Warren, Bishop, J. Michael
The deoxyribonucleic acid (DNA) polymerase of Rous sarcoma virus synthesizes both single- and double-stranded DNA, utilizing the ribonucleic acid (RNA) of the viral genome as the initial template....
Quintrell, Nancy, Fanshier, Lois, Evans, Barbara, Levinson, Warren, Bishop, J. Michael
Purified preparations of Rous sarcoma virus (RSV) contain ribonuclease which is either a constituent of the virion surface or an adsorbed contaminant. Treatment of the virus with nonionic detergent...
Leong, Jo-Ann, Garapin, Axel-Claude, Jackson, Nola, Fanshier, Lois, Levinson, Warren, Bishop, J. Michael
Cells producing Rous sarcoma virus contain virus-specific ribonucleic acid (RNA) which can be identified by hybridization to single-stranded deoxyribonucleic acid (DNA) synthesized with RNA-directed...
Transcripts from the Cellular Homologs of Retroviral Oncogenes: Distribution Among Chicken Tissues
Gonda, Thomas J., Sheiness, Diana K., Bishop, J. Michael
The oncogenes (v-onc genes) of rapidly transforming retroviruses have homologs (c-onc genes) in the genomes of normal cells. In this study, we characterized and quantitated transcription from four...
Garapin, Axel-Claude, McDonnell, Jerome P., Levinson, Warren, Quintrell, Nancy, Fanshier, Lois, Bishop, J. Michael
Deoxyribonucleic acid (DNA) polymerase activity can be elicited in purified preparations of avian myeloblastosis virus and Rous sarcoma virus (Schmidt-Ruppin strain) by treatment with nonionic...
Varmus, Harold E., Vogt, Peter K., Bishop, J. Michael
A relatively simple but stringent technique was developed to detect the integration of virus-specific DNA into the genomes of higher organisms. In both permissive (duck) and nonpermissive (mammalian)...
Detection of Avian Tumor Virus-Specific Nucleotide Sequences in Avian Cell DNAs
Varmus, Harold E., Weiss, Robin A., Friis, Robert R., Levinson, Warren, Bishop, J. Michael
The effect of unlabeled cellular DNA upon the reassociation kinetics of labeled double-stranded DNA made by DNA polymerase from avian tumor viruses has been used to measure virus-specific nucleotide...
Levinson, Warren, Faras, Anthony, Woodson, Bruce, Jackson, Jean, Bishop, J. Michael
The RNA-dependent DNA polymerase of Rous sarcoma virus is inhibited by N-methyl isatin β-thiosemicarbazone and by thiosemicarbazide, but not by semicarbazide. These inhibitors also inactivate, upon...
Varmus, Harold E., Guntaka, Ramareddy V., Fan, Warner J. W., Heasley, Suzanne, Bishop, J. Michael
Two lines of evidence indicate that synthesis of viral DNA occurs in the cytoplasm of duck embryo fibroblasts infected with avian sarcoma virus: (i) viral DNA is detected first in the cytoplasmic...
Cell division and cell survival in the absence of survivin
Yang, Dun, Welm, Alana, Bishop, J. Michael
The survivin protein contains structural features of the inhibitor of apoptosis protein family. Previous studies have suggested that survivin is essential for cell survival because it counteracts an...
env Gene of Chicken RNA Tumor Viruses: Extent of Conservation in Cellular and Viral Genomes
Fujita, Donald J., Tal, Jacov, Varmus, Harold E., Bishop, J. Michael
The env gene of avian sarcoma-leukosis viruses codes for envelope glycoproteins that determine viral host range, antigenic specificity, and interference patterns. We used molecular hybridization to...
Kim, Suwon, Chin, Koei, Gray, Joe W., Bishop, J. Michael
We have devised a screen for genes that suppress the loss of contact inhibition elicited by overexpression of the protooncogene MYCN. The initial application of this screen detected nine distinctive...
Buchholz, Frank, Refaeli, Yosef, Trumpp, Andreas, Bishop, J. Michael
Transgenic mice have been used to explore the role of chromosomal translocations in the genesis of tumors. But none of these efforts has actually involved induction of a translocation in vivo. Here...
Hahn, Mounou, Bishop, J. Michael
The RET gene, encoding a receptor tyrosine kinase, is unusual among human protooncogenes in that its mutant alleles are implicated in a developmental defect involving enteric neurons as well as in...
A PMLRARα transgene initiates murine acute promyelocytic leukemia
Brown, Diane, Kogan, Scott, Lagasse, Eric, Weissman, Irving, Alcalay, Myriam, Pelicci, Pier Giuseppe, ...
The malignant cells of acute promyelocytic leukemia (APL) contain a reciprocal chromosomal translocation that fuses the promyelocytic leukemia gene (PML) with the retinoic acid receptor α gene...
Identification of a large Myc-binding protein that contains RCC1-like repeats
Guo, Qingbin, Xie, Jingwu, Dang, Chi V., Liu, Edison T., Bishop, J. Michael
The protooncogene MYC plays an important role in the regulation of cellular proliferation, differentiation, and apoptosis and has been implicated in a variety of human tumors. MYC and the closely...
Kogan, Scott C., Lagasse, Eric, Atwater, Susan, Bae, Suk-chul, Weissman, Irving, Ito, Yoshiaki, ...
Chromosomal translocations involving the genes encoding the alpha and beta subunits of the Pebp2/Cbf transcription factor have been associated with human acute myeloid leukemia and the preleukemic...
Smith-McCune, K., Kalman, D., Robbins, C., Shivakumar, S., Yuschenkoff, L., Bishop, J. Michael
To study intracellular pathways by which the human papillomavirus 16 oncogene E7 participates in carcinogenesis, we expressed an inducible chimera of E7 by fusion to the hormone-binding domain of the...
Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening
Zhu, Jiyue, Wang, He, Bishop, J. Michael, Blackburn, Elizabeth H.
Human fibroblasts whose lifespan in culture has been extended by expression of a viral oncogene eventually undergo a growth crisis marked by failure to proliferate. It has been proposed that telomere...
Transient excess of MYC activity can elicit genomic instability and tumorigenesis
Felsher, Dean W., Bishop, J. Michael
Overexpression of the MYC protooncogene has been implicated in the genesis of diverse human tumors. Tumorigenesis induced by MYC has been attributed to sustained effects on proliferation and...
Src interacts with dynamin and synapsin in neuronal cells
Foster-Barber, Audrey, Bishop, J. Michael
The nonreceptor tyrosine kinase Src is expressed at a high level in cells that are specialized for regulated secretion, such as the neuron, and is concentrated on secretory vesicles or at the site of...
Radany, Eric H., Hong, Karen, Kesharvarzi, Sima, Lander, Eric S., Bishop, J. Michael
Hybrid mice carrying oncogenic transgenes afford powerful systems for investigating loss of heterozygosity (LOH) in tumors. Here, we apply this approach to a neoplasm of key importance in human...
Overexpression of MYC causes p53-dependent G2 arrest of normal fibroblasts
Felsher, Dean W., Zetterberg, Anders, Zhu, Jiyue, Tlsty, Thea, Bishop, J. Michael
Overexpression of the proto-oncogene MYC has been implicated in the genesis of diverse human cancers. One explanation for the role of MYC in tumorigenesis has been that this gene might drive cells...
Ras Family GTPases Control Growth of Astrocyte Processes
Kalman, Daniel, Gomperts, Stephen N., Hardy, Stephen, Kitamura, Marina, Bishop, J. Michael
Astrocytes in neuron-free cultures typically lack processes, although they are highly process-bearing in vivo. We show that basic fibroblast growth factor (bFGF) induces cultured astrocytes to grow...
Cheng, Steven Yan, Bishop, J. Michael
The Suppressor of Fused [Su(fu)] protein plays a conserved role in the regulation of Gli transcription factors of the hedgehog (Hh) signaling pathway that controls cell fate and tissue patterning...
Yang, Dun, Buchholz, Frank, Huang, Zhongdong, Goga, Andrei, Chen, Chih-Ying, Brodsky, Frances M., ...
Small interfering RNA (siRNA) has become a powerful tool for selectively silencing gene expression in cultured mammalian cells. Because different siRNAs of the same gene have variable silencing...
Proposal for Naming Host Cell-Derived Inserts in Retrovirus Genomes †
Coffin, John M., Varmus, Harold E., Bishop, J. Michael, Essex, Myron, Hardy, William D., Martin, G. Steven, ...
We propose a system for naming inserted sequences in transforming retroviruses (i.e., onc genes), based on using trivial names derived from a prototype strain of virus.
Sheiness, Diana, Bister, Klaus, Moscovici, Carlo, Fanshier, Lois, Gonda, Thomas, Bishop, J. Michael
Avian myelocytomatosis virus (MC29V) is a retrovirus that transforms both fibroblasts and macrophages in culture and induces myelocytomatosis, carcinomas, and sarcomas in birds. Previous work...
Drosophila myb is required for the G2/M transition and maintenance of diploidy
Katzen, Alisa L., Jackson, Jean, Harmon, Brian P., Fung, Siau-Min, Ramsay, Gary, Bishop, J. Michael
The myb proto-oncogenes are thought to have a role in the cell division cycle. We have examined this possibility by genetic analysis in Drosophila melanogaster, which possesses a single myb gene. We...
Trumpp, Andreas, Depew, Michael J., Rubenstein, John L.R., Bishop, J. Michael, Martin, Gail R.
In mammals, the first branchial arch (BA1) develops into a number of craniofacial skeletal elements including the jaws and teeth. Outgrowth and patterning of BA1 during early embryogenesis is thought...
Senescence of human fibroblasts induced by oncogenic Raf
Zhu, Jiyue, Woods, Douglas, McMahon, Martin, Bishop, J. Michael
The oncogenes RAS and RAF came to view as agents of neoplastic transformation. However, in normal cells, these genes can have effects that run counter to oncogenic transformation, such as arrest of...
Negative Control of the Myc Protein by the Stress-Responsive Kinase Pak2
Huang, Zhongdong, Traugh, Jolinda A., Bishop, J. Michael
Pak2 is a serine/threonine kinase that participates in the cellular response to stress. Among the potential substrates for Pak2 is the protein Myc, encoded by the proto-oncogene MYC. Here we...
Sheiness, Diana, Bishop, J. Michael
The avian carcinoma virus MC29 (MC29V) contains a sequence of approximately 1,500 nucleotides which may represent a gene responsible for tumorigenesis by MC29V. We present evidence that MC29V has...
Varmus, Harold E., Heasley, Suzanne, Bishop, J. Michael
Labeled, virus-specific DNA synthesized in vitro by the virion-associated polymerase of avian sarcoma virus (ASV) was used to measure virus-specific sequences in cell DNA in three ways: (i) by...
Fanshier, Lois, Garapin, Axel-Claude, McDonnell, Jerome, Faras, Anthony, Levinson, Warren, Bishop, J. Michael
The products of the deoxyribonucleic acid (DNA) polymerase associated with Rous sarcoma virus and avian myeloblastosis virus were characterized by correlative analyses with equilibrium centrifugation...
Garapin, Axel-Claude, Fanshier, Lois, Leong, Jo-Ann, Jackson, Jean, Levinson, Warren, Bishop, J. Michael
The deoxyribonucleic acid (DNA) polymerase(s) of Rous sarcoma virus synthesizes two principal products—single-stranded DNA in the form of a DNA:ribonucleic acid (RNA) hybrid and double-stranded...
Faras, Anthony, Fanshier, Lois, Garapin, Axel-Claude, Levinson, Warren, Bishop, J. Michael
The deoxyribonucleic acid (DNA) polymerase of Rous sarcoma virus synthesizes both single- and double-stranded DNA, utilizing the ribonucleic acid (RNA) of the viral genome as the initial template....
Quintrell, Nancy, Fanshier, Lois, Evans, Barbara, Levinson, Warren, Bishop, J. Michael
Purified preparations of Rous sarcoma virus (RSV) contain ribonuclease which is either a constituent of the virion surface or an adsorbed contaminant. Treatment of the virus with nonionic detergent...