Krehenbrink, Martin, Downie, J Allan
Abstract Background Proteins secreted by bacteria play an important role in infection of eukaryotic hosts. Rhizobia infect the roots of leguminous plants and establish a mutually beneficial...
Protein domains and architectural innovation in plant-associated Proteobacteria (2005)
Studholme, David J, Downie, J Allan, Preston, Gail M
Abstract Background Evolution of new complex biological behaviour tends to arise by novel combinations of existing building blocks. The functional and evolutionary building blocks of the proteome are...
Protein domains and architectural innovation in plant-associated Proteobacteria (2005)
Studholme, David J, Downie, J Allan, Preston, Gail M
Background Evolution of new complex biological behaviour tends to arise by novel combinations of existing building blocks. The functional and evolutionary building blocks of the proteome are protein...
Walker, Simon A., Viprey, Virginie, Downie, J. Allan
Changes in intracellular calcium in pea root hairs responding to Rhizobium leguminosarum bv. viciae nodulation (Nod) factors were analyzed by using a microinjected calcium-sensitive fluorescent dye...
Rodelas, Belen, Lithgow, James K., Wisniewski-Dye, Florence, Hardman, Andrea, Wilkinson, Adam, Economou, Anastassios, ...
The rhi genes of Rhizobium leguminosarum biovar viciae are expressed in the rhizosphere and play a role in the interaction with legumes, such as the pea. Previously (K. M. Gray, J. P. Pearson, J. A....
Zorreguieta, Angeles, Finnie, Christine, Downie, J. Allan
Rhizobium leguminosarum secretes two extracellular glycanases, PlyA and PlyB, that can degrade exopolysaccharide (EPS) and carboxymethyl cellulose (CMC), which is used as a model substrate of plant...
Enhanced Symbiotic Performance by Rhizobium tropici Glycogen Synthase Mutants
Marroquí, Silvia, Zorreguieta, Angeles, Santamaría, Carmen, Temprano, Francisco, Soberón, Mario, Megías, Manuel, ...
We isolated a Tn5-induced Rhizobium tropici mutant that has enhanced capacity to oxidize N,N-dimethyl-p-phenylendiamine (DMPD) and therefore has enhanced respiration via cytochrome oxidase. The...
Lloret, Javier, Wulff, Brande B. H., Rubio, Jose M., Downie, J. Allan, Bonilla, Ildefonso, Rivilla, Rafael
The halotolerant strain Rhizobium meliloti EFB1 modifies the production of extracellular polysaccharides in response to salt. EFB1 colonies grown in the presence of 0.3 M NaCl show a decrease in...
Finnie, Christine, Zorreguieta, Angeles, Hartley, Nigel M., Downie, J. Allan
The prsDE genes encode a type I protein secretion system required for the secretion of the nodulation protein NodO and at least three other proteins from Rhizobium leguminosarum bv. viciae. At least...
Energy Supply for Active Transport in Anaerobically Grown Escherichia coli
Boonstra, Johannes, Downie, J. Allan, Konings, Wil N.
Escherichia coli K-12, grown under anaerobic conditions with glucose as the sole source of carbon and energy without any terminal electron acceptor added, contains a fumarate reductase system in...
Downie, J. Allan, Langman, Lyndall, Cox, Graeme B., Yanofsky, Charles, Gibson, Frank
The unc operon of Escherichia coli was split into two fragments by the restriction endonuclease HindIII. The operator-proximal portion was cloned into plasmid pACYC184, forming plasmid pAN51, which...
Mitra, Raka M., Gleason, Cynthia A., Edwards, Anne, Hadfield, James, Downie, J. Allan, Oldroyd, Giles E. D., ...
In the establishment of the legume-rhizobial symbiosis, bacterial lipochitooligosaccharide signaling molecules termed Nod factors activate the formation of a novel root organ, the nodule. Nod factors...
Downie, J.Allan, Ma, Qing-Sheng, Knight, Celia D., Hombrecher, Gerd, Johnston, Andrew W.B.
The region of the Rhizobium leguminosarum plasmid pRL1JI involved in nodulation and nitrogen fixation has been cloned on a series of four overlapping cosmid clones. These clones represent ˜60 kb of...
Haddock, Bruce A., Downie, J. Allan
Measurements were made of electron-transport-dependent quenching of atebrin fluorescence in particles prepared from mutants of Escherichia coli unable to synthesize either ubiquinone or haem. Such...
Kanamori, Norihito, Madsen, Lene Heegaard, Radutoiu, Simona, Frantescu, Mirela, Quistgaard, Esben M. H., Miwa, Hiroki, ...
Nuclear-cytoplasmic partitioning and traffic between cytoplasmic and nuclear compartments are fundamental processes in eukaryotic cells. Nuclear pore complexes mediate transport of proteins, RNAs and...
Russo, Daniela M., Williams, Alan, Edwards, Anne, Posadas, Diana M., Finnie, Christine, Dankert, Marcelo, ...
The type I protein secretion system of Rhizobium leguminosarum bv. viciae encoded by the prsD and prsE genes is responsible for secretion of the exopolysaccharide (EPS)-glycanases PlyA and PlyB. The...
Walker, Simon A., Viprey, Virginie, Downie, J. Allan
Changes in intracellular calcium in pea root hairs responding to Rhizobium leguminosarum bv. viciae nodulation (Nod) factors were analyzed by using a microinjected calcium-sensitive fluorescent dye...
Rodelas, Belen, Lithgow, James K., Wisniewski-Dye, Florence, Hardman, Andrea, Wilkinson, Adam, Economou, Anastassios, ...
The rhi genes of Rhizobium leguminosarum biovar viciae are expressed in the rhizosphere and play a role in the interaction with legumes, such as the pea. Previously (K. M. Gray, J. P. Pearson, J. A....
Zorreguieta, Angeles, Finnie, Christine, Downie, J. Allan
Rhizobium leguminosarum secretes two extracellular glycanases, PlyA and PlyB, that can degrade exopolysaccharide (EPS) and carboxymethyl cellulose (CMC), which is used as a model substrate of plant...
Enhanced Symbiotic Performance by Rhizobium tropici Glycogen Synthase Mutants
Marroquí, Silvia, Zorreguieta, Angeles, Santamaría, Carmen, Temprano, Francisco, Soberón, Mario, Megías, Manuel, ...
We isolated a Tn5-induced Rhizobium tropici mutant that has enhanced capacity to oxidize N,N-dimethyl-p-phenylendiamine (DMPD) and therefore has enhanced respiration via cytochrome oxidase. The...
Lloret, Javier, Wulff, Brande B. H., Rubio, Jose M., Downie, J. Allan, Bonilla, Ildefonso, Rivilla, Rafael
The halotolerant strain Rhizobium meliloti EFB1 modifies the production of extracellular polysaccharides in response to salt. EFB1 colonies grown in the presence of 0.3 M NaCl show a decrease in...
Finnie, Christine, Zorreguieta, Angeles, Hartley, Nigel M., Downie, J. Allan
The prsDE genes encode a type I protein secretion system required for the secretion of the nodulation protein NodO and at least three other proteins from Rhizobium leguminosarum bv. viciae. At least...
Energy Supply for Active Transport in Anaerobically Grown Escherichia coli
Boonstra, Johannes, Downie, J. Allan, Konings, Wil N.
Escherichia coli K-12, grown under anaerobic conditions with glucose as the sole source of carbon and energy without any terminal electron acceptor added, contains a fumarate reductase system in...
Downie, J. Allan, Langman, Lyndall, Cox, Graeme B., Yanofsky, Charles, Gibson, Frank
The unc operon of Escherichia coli was split into two fragments by the restriction endonuclease HindIII. The operator-proximal portion was cloned into plasmid pACYC184, forming plasmid pAN51, which...
Mitra, Raka M., Gleason, Cynthia A., Edwards, Anne, Hadfield, James, Downie, J. Allan, Oldroyd, Giles E. D., ...
In the establishment of the legume-rhizobial symbiosis, bacterial lipochitooligosaccharide signaling molecules termed Nod factors activate the formation of a novel root organ, the nodule. Nod factors...
Downie, J.Allan, Ma, Qing-Sheng, Knight, Celia D., Hombrecher, Gerd, Johnston, Andrew W.B.
The region of the Rhizobium leguminosarum plasmid pRL1JI involved in nodulation and nitrogen fixation has been cloned on a series of four overlapping cosmid clones. These clones represent ˜60 kb of...
Haddock, Bruce A., Downie, J. Allan
Measurements were made of electron-transport-dependent quenching of atebrin fluorescence in particles prepared from mutants of Escherichia coli unable to synthesize either ubiquinone or haem. Such...
Kanamori, Norihito, Madsen, Lene Heegaard, Radutoiu, Simona, Frantescu, Mirela, Quistgaard, Esben M. H., Miwa, Hiroki, ...
Nuclear-cytoplasmic partitioning and traffic between cytoplasmic and nuclear compartments are fundamental processes in eukaryotic cells. Nuclear pore complexes mediate transport of proteins, RNAs and...
Russo, Daniela M., Williams, Alan, Edwards, Anne, Posadas, Diana M., Finnie, Christine, Dankert, Marcelo, ...
The type I protein secretion system of Rhizobium leguminosarum bv. viciae encoded by the prsD and prsE genes is responsible for secretion of the exopolysaccharide (EPS)-glycanases PlyA and PlyB. The...
Heckmann, Anne B., Lombardo, Fabien, Miwa, Hiroki, Perry, Jillian A., Bunnewell, Sue, Parniske, Martin, ...
A new nodulation-defective mutant of Lotus japonicus does not initiate nodule cortical cell division in response to Mesorhizobium loti, but induces root hair deformation, Nod factor-induced calcium...
Saito, Katsuharu, Yoshikawa, Makoto, Yano, Koji, Miwa, Hiroki, Uchida, Hisaki, Asamizu, Erika, ...
In Lotus japonicus, seven genetic loci have been identified thus far as components of a common symbiosis (Sym) pathway shared by rhizobia and arbuscular mycorrhizal fungi. We characterized the nup85...
Sun, Jongho, Miwa, Hiroki, Downie, J. Allan, Oldroyd, Giles E.D.
The rhizobial-derived signaling molecule Nod factor is essential for the establishment of the Medicago truncatula/Sinorhizobium meliloti symbiosis. Nod factor perception and signal transduction in...
Crossman, Lisa C., Castillo-Ramírez, Santiago, McAnnula, Craig, Lozano, Luis, Vernikos, Georgios S., Acosta, José L., ...
This work centres on the genomic comparisons of two closely-related nitrogen-fixing symbiotic bacteria, Rhizobium leguminosarum biovar viciae 3841 and Rhizobium etli CFN42. These strains maintain a...
The Medicago truncatula DMI1 Protein Modulates Cytosolic Calcium Signaling1[W][OA]
Peiter, Edgar, Sun, Jongho, Heckmann, Anne B., Venkateshwaran, Muthusubramanian, Riely, Brendan K., Otegui, Marisa S., ...
In addition to establishing symbiotic relationships with arbuscular mycorrhizal fungi, legumes also enter into a nitrogen-fixing symbiosis with rhizobial bacteria that results in the formation of...
Williams, Alan, Wilkinson, Adam, Krehenbrink, Martin, Russo, Daniela M., Zorreguieta, Angeles, Downie, J. Allan
The Rhizobium leguminosarum biovar viciae genome contains several genes predicted to determine surface polysaccharides. Mutants predicted to affect the initial steps of polysaccharide synthesis were...