Isao Nagano

Trichinella spiralis: nurse cell formation with emphasis on analogy to muscle cell repair (2008)

Wu, Zhiliang, Sofronic-Milosavljevic, Lj, Nagano, Isao, Takahashi, Yuzo

Abstract Trichinella infection results in formation of a capsule in infected muscles. The capsule is a residence of the parasite which is composed of the nurse cell and fibrous wall. The process of...

MOLECULAR COMPARATIVE STUDIES AMONG BLASTOCYSTIS ISOLATES OBTAINED FROM HUMANS AND ANIMALS (2003)

Hisao Yoshikawa, Zhiliang Wu, Isao Nagano, Yuzo Takahashi

This study compared specific polymerase chain reaction (PCR) primers and phylogenetic tree analysis of restriction fragment length polymorphism using the small subunit ribosomal RNA gene in various...

MOLECULAR CLONING AND CHARACTERIZATION OF A SERINE PROTEINASE GENE OF TRICHINELLA SPIRALIS (2003)

Isao Nagano, Zhiliang Wu, Takumi Nakada, Thidarut Boonmars, Yuzo Takahashi

A serine proteinase gene was isolated from a cDNA library of Trichinella spiralis muscle larvae at 30 days postinfection (PI). The library was immunoscreened with T. spiralis–infected sera. A...

Genomic Analysis of Blastocystis hominis Strains Isolated from Two Long-Term Health Care Facilities

Yoshikawa, Hisao, Abe, Niichiro, Iwasawa, Mizue, Kitano, Syoko, Nagano, Isao, Wu, Zhiliang, ...

The genotype Blastocystis hominis is highly polymorphic. Therefore, a genetic marker would be a powerful tool for the identification or classification of B. hominis subtypes and could be used as a...

Intraspecies Polymorphism of Cryptosporidium parvum Revealed by PCR-Restriction Fragment Length Polymorphism (RFLP) and RFLP-Single-Strand Conformational Polymorphism Analyses

Wu, Zhiliang, Nagano, Isao, Boonmars, Thidarut, Nakada, Takumi, Takahashi, Yuzo

A glycoprotein (Cpgp40/15)-encoding gene of Cryptosporidium parvum was analyzed to reveal intraspecies polymorphism within C. parvum isolates. Forty-one isolates were collected from different...

Molecular Expression of a Cysteine Proteinase of Clonorchis sinensis and Its Application to an Enzyme-Linked Immunosorbent Assay for Immunodiagnosis of Clonorchiasis

Nagano, Isao, Pei, Fuquan, Wu, Zhiliang, Wu, Jun, Cui, Huier, Boonmars, Thidarut, ...

We produced a recombinant cysteine proteinase of Clonorchis sinensis and tested its value as an antigen for serologic diagnosis of C. sinensis infections. The predicted amino acid sequence of the...

Genomic Analysis of Blastocystis hominis Strains Isolated from Two Long-Term Health Care Facilities

Yoshikawa, Hisao, Abe, Niichiro, Iwasawa, Mizue, Kitano, Syoko, Nagano, Isao, Wu, Zhiliang, ...

The genotype Blastocystis hominis is highly polymorphic. Therefore, a genetic marker would be a powerful tool for the identification or classification of B. hominis subtypes and could be used as a...

Intraspecies Polymorphism of Cryptosporidium parvum Revealed by PCR-Restriction Fragment Length Polymorphism (RFLP) and RFLP-Single-Strand Conformational Polymorphism Analyses

Wu, Zhiliang, Nagano, Isao, Boonmars, Thidarut, Nakada, Takumi, Takahashi, Yuzo

A glycoprotein (Cpgp40/15)-encoding gene of Cryptosporidium parvum was analyzed to reveal intraspecies polymorphism within C. parvum isolates. Forty-one isolates were collected from different...

Molecular Expression of a Cysteine Proteinase of Clonorchis sinensis and Its Application to an Enzyme-Linked Immunosorbent Assay for Immunodiagnosis of Clonorchiasis

Nagano, Isao, Pei, Fuquan, Wu, Zhiliang, Wu, Jun, Cui, Huier, Boonmars, Thidarut, ...

We produced a recombinant cysteine proteinase of Clonorchis sinensis and tested its value as an antigen for serologic diagnosis of C. sinensis infections. The predicted amino acid sequence of the...

Species-Specific Antibody Responses to the Recombinant 53-Kilodalton Excretory and Secretory Proteins in Mice Infected with Trichinella spp.▿

Nagano, Isao, Wu, Zhiliang, Takahashi, Yuzo

The 53-kDa proteins in larval excretory and secretory (E-S) products were expressed from five Trichinella species (T. spiralis, T. britovi, T. nativa, T. pseudospiralis, and T. papuae), using the...

Trichinella spiralis: nurse cell formation with emphasis on analogy to muscle cell repair

Wu, Zhiliang, Sofronic-Milosavljevic, Lj, Nagano, Isao, Takahashi, Yuzo

Trichinella infection results in formation of a capsule in infected muscles. The capsule is a residence of the parasite which is composed of the nurse cell and fibrous wall. The process of nurse cell...