Blumenstein, Lars, Domratcheva, Tatiana, Niks, Dimitri, Ngo, Huu, Seidel, Ralf, Dunn, Michael F, ...
Makris, T. M., Von König, Konstanze, Schlichting, Ilme, Sligar, S. G.
Distal pocket water molecules have been widely implicated in the delivery of protons required in O−O bond heterolysis in the P450 reaction cycle. Targeted dehydration of the cytochrome P450cam...
Blumenstein, Lars, Domratcheva, Tatiana, Niks, Dimitri, Ngo, Huu, Seidel, Ralf, Dunn, Michael F., ...
In the PLP−requiring α2α2 tryptophan synthase complex, recognition of the substrate L−Ser at the β−site includes a loop structure (residues β110−115) extensively H−bonded to the...
Ngo, Huu, Harris, Rodney, Kimmich, Novelle, Casino, Patricia, Niks, Dimitri, Blumenstein, Lars, ...
Allosteric interactions regulate substrate channeling in Salmonella typhimurium tryptophan synthase. The channeling of indole between the Alpha− and ß−sites via the interconnecting 25 Å...
Ngo, H., Kimmich, N., Harris, R., Niks, D., Blumenstein, Lars, Kulik, Victor, ...
n the tryptophan synthase bienzyme complex, indole produced by substrate cleavage at the Alpha−site is channeled to the ß−site via a 25 Å long tunnel. Within the ß−site, indole and...
Casino, P., Niks, D., Ngo, H., Pan, P., Brzovic, P., Blumenstein, Lars, ...
Substrate channeling in the tryptophan synthase bienzyme complex from Salmonella typhimurium is regulated by allosteric interactions triggered by binding of ligand to the −site and covalent...
Beitlich, Thorsten, Kühnel, Karin, Schulze−Briese, Clemens, Shoeman, Robert L., Schlichting, Ilme
The X−ray crystallographic analysis of redox−active systems may be complicated by photoreduction. Although radiolytic reduction by the probing X−ray beam may be exploited to generate otherwise...
Crystal Structure of the Human Retinitis Pigmentosa 2 Protein and Its Interaction with Arl3 (2006)
Kühnel,Karin, Veltel,Stefan, Schlichting,Ilme, Wittinghofer,Alfred
Crystal Structure of the Human Retinitis Pigmentosa 2 Protein and Its Interaction with Arl3 (2006)
Kühnel, Karin, Veltel, Stefan, Schlichting, Ilme, Wittinghofer, Alfred
Blankenfeldt, Wulf, Thomae, Nicolas H., Wray, John, Gautel, Mathias, Schlichting, Ilme
Myosin II is the major component of the muscle thick filament. It consists of two N−terminal S1 subfragments (heads) connected to a long dimeric coiled−coil rod. The rod is in itself twofold...
Jung, Astrid, Reinstein, Jochen, Domratcheva, Tatiana, Shoeman, Robert L., Schlichting, Ilme
Proteins containing a sensor of blue light using FAD (BLUF) domain control diverse cellular processes, such as gene expression, nucleotide metabolism and motility, by relaying blue light signals to...
Kühnel, Karin, Blankenfeldt, Wulf, Terner, James, Schlichting, Ilme
Chloroperoxidase (CPO) is a heme−thiolate enzyme catalyzing hydrogen peroxide dependent halogenation reactions. Structural data on substrate binding have not been available so far. CPO was...
Crystal Structure of the Human Retinitis Pigmentosa 2 Protein and Its Interaction with Arl3 (2006)
Kühnel, Karin, Veltel, Stefan, Schlichting, Ilme, Wittinghofer, Alfred
The crystal structure of human retinitis pigmentosa 2 protein (RP2) was solved to 2.1 Å resolution. It consists of an N−terminal β helix and a C−terminal ferredoxin−like α/β domain. RP2 is...
Photodynamics of the small BLUF protein BlrB from Rhodobacter sphaeroides (2006)
Zirak, P., Penzkofer, A., Schiereis, T., Hegemann, P., Jung, Astrid, Schlichting, Ilme
The BLUF protein BlrB from the non−sulphur anoxyphototrophic purple bacterium Rhodobacter sphaeroides is characterized by absorption and emission spectroscopy. BlrB expressed from E. coli binding...
The status of high−valent metal oxo complexes in the P450 cytochromes (2006)
Makris, Thomas M., Von König, Konstanze, Schlichting, Ilme, Sligar, Stephen G.
The oxidative prowess of the P450 cytochromes in physiological reactions is attributed to the production of a high−valent iron−oxo complex, or Compound I intermediate, in the reaction cycle....
Kulik, Victor, Hartmann, Elisabeth, Weyand, Michael, Frey, Monika, Gierl, Alfons, Niks, Dimitri, ...
Zirak, P., Penzkofer, A., Schiereis, T., Hegemann, P., Jung, Astrid, Schlichting, Ilme
The BLUF domain of the transcriptional anti−repressor protein AppA from the non−sulfur anoxyphototrophic purple bacterium Rhodobacter sphaeroides was characterized by absorption and emission...
Production and X−ray crystallographic analysis of fully deuterated cytochrome P450cam (2005)
Meilleur, Flora, Dauvergne, Marie−Thérèse, Schlichting, Ilme, Myles, Dean
Neutron protein crystallography allows H−atom positions to be located in biological structures at the relatively modest resolution of 1.5−2.0 Å. A difficulty of this technique arises from the...
Matter, Hans, Kumar, H. S. Arun, Fedorov, Roman, Frey, Armin, Kotsonis, Peter, Hartmann, Elisabeth, ...
Nitric oxide synthesized from L−arginine by nitric oxide synthase isoforms (NOS−I−III) is physiologically important but also can be deleterious when overproduced. Selective NOS inhibitors are...
Structure and Chemistry of Cytochrome P450 (2005)
Denisov, Ilia G., Makris, Thomas M., Sligar, Stephen G., Schlichting, Ilme
The title to a seminar presentation by I. C. Gunsalus in 1973 was Oxygen: An essential toxin, referring to the complex role that atmospheric dioxygen has in biology. The relatively simple function as...
Kulik, Victor, Hartmann, Elisabeth, Weyand, Michael, Frey, Marco, Gierl, Alfons, ...
Indole is a reaction intermediate in at least two biosynthetic pathways in maize seedlings. In the primary metabolism, the α−subunit (TSA) of the bifunctional tryptophan synthase (TRPS) catalyzes...
Jung, Astrid, Domratcheva, Tatjana, Tarutina, Marina, Wu, Qiong, Ko, Wen−Huang, Shoeman, Robert L., ...
Light is an essential environmental factor, and many species have evolved the capability to respond to it. Blue light is perceived through three flavin−containing photoreceptor families:...
Kulik, Victor, Hartmann, Elisabeth, Weyand, Michael, Frey, Marco, Gierl, Alfons, ...
Indole is a reaction intermediate in at least two biosynthetic pathways in maize seedlings. In the primary metabolism, the α−subunit (TSA) of the bifunctional tryptophan synthase (TRPS) catalyzes...
The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat (2004)
Kühnel,Karin, Jarchau,Thomas, Wolf,Eva, Schlichting,Ilme, Ulrich,Walter, Wittinghofer,Alfred, ...
The VASP tetramerization domain is a right−handed coiled coil based on a 15−residue repeat (2004)
Kühnel,Karin, Jarchau,Thomas, Wolf,Eva, Schlichting,Ilme, Walter,Ulrich, Wittinghofer,Alfred, ...
The vasodilator−stimulated phosphoprotein (VASP) is a key regulator of actin dynamics. We have determined the 1.3−Å resolution crystal structure of the 45−residue−long tetramerization domain...
Time−resolved imaging of macromolecular processes and interactions (2004)
Frank,Joachim, Schlichting,Ilme
Cryo−EM−especially single−particle cryo−EM−and X−ray crystallography are increasingly paired in studies of macromolecular assemblies. The ensuing so−called hybrid methods of structure...
Structural Aspects of Ligand Binding to and Electron Transfer in Bacterial and Fungal P450S (2004)
Pylypenko,Olena, Schlichting,Ilme
Cytochrome P450 enzymes are heme−containing monooxygenases that are named after an absorption band at 450 nm when complexed with carbon monoxide. They catalyze a wide variety of reactions and are...
A survey of active site access channels in cytochromes P450 (2004)
Wade,Rebecca C., Winn,Peter J., Schlichting,Ilme, Sudarko,S.
In cytochrome P450s, the active site is situated deep inside the protein next to the heme cofactor, and is often completely isolated from the surrounding solvent. To identify routes by which...
A survey of active site access channels in cytochromes P450 (2004)
Wade,Rebecca C., Winn,Peter J., Schlichting,Ilme, Sudarko,Sudarko
Fedorov,Roman, Vasan,Ryan, Gosh,Dipak K., Schlichting,Ilme
The high level of amino acid conservation and structural similarity of the substrate−binding sites of the oxygenase domains of the nitric oxide synthase (NOS) isoforms (eNOSoxy, iNOSoxy, nNOSoxy)...
The VASP tetramerization domain is a right-handed coiled coil based on a 15-residue repeat (2004)
Kühnel, Karin, Jarchau, Thomas, Wolf, Eva, Schlichting, Ilme, Ulrich, Walter, Wittinghofer, Alfred, ...
A survey of active site access channels in cytochromes P450 (2004)
Wade, Rebecca C., Winn, Peter J., Schlichting, Ilme, Sudarko, Sudarko
Fedorov, Roman, Vasan, Ryan, Gosh, Dipak K., Schlichting, Ilme
The high level of amino acid conservation and structural similarity of the substrate−binding sites of the oxygenase domains of the nitric oxide synthase (NOS) isoforms (eNOSoxy, iNOSoxy, nNOSoxy)...
The VASP tetramerization domain is a right−handed coiled coil based on a 15−residue repeat (2004)
Kühnel, Karin, Jarchau, Thomas, Wolf, Eva, Schlichting, Ilme, Walter, Ulrich, Wittinghofer, Alfred, ...
The vasodilator−stimulated phosphoprotein (VASP) is a key regulator of actin dynamics. We have determined the 1.3−Å resolution crystal structure of the 45−residue−long tetramerization domain...
Time−resolved imaging of macromolecular processes and interactions (2004)
Frank, Joachim, Schlichting, Ilme
Cryo−EM−especially single−particle cryo−EM−and X−ray crystallography are increasingly paired in studies of macromolecular assemblies. The ensuing so−called hybrid methods of structure...
Structural Aspects of Ligand Binding to and Electron Transfer in Bacterial and Fungal P450S (2004)
Pylypenko, Olena, Schlichting, Ilme
Cytochrome P450 enzymes are heme−containing monooxygenases that are named after an absorption band at 450 nm when complexed with carbon monoxide. They catalyze a wide variety of reactions and are...
A survey of active site access channels in cytochromes P450 (2004)
Wade, Rebecca C., Winn, Peter J., Schlichting, Ilme, Sudarko, S.
In cytochrome P450s, the active site is situated deep inside the protein next to the heme cofactor, and is often completely isolated from the surrounding solvent. To identify routes by which...
Pylypenko,Olena, Vitali,Francesca, Zerbe,Katja, Robinson,John A., Schlichting,Ilme
Gene inactivation studies point to the involvement of OxyC in catalyzing the last oxidative phenol coupling reaction during glycopeptide antibiotic biosynthesis. Presently, the substrate and exact...
Federov,Roman, Hartmann,Elisabeth, Ghosh,Dipak K., Schlichting,Ilme
The high level of amino acid conservation and structural similarity in the immediate vicinity of the substrate binding sites of the oxygenase domains of the nitric-oxide synthase (NOS) isoforms...
Irreversible Photoreduction of Flavin in a Mutated Phot-LOV1 Domain (2003)
Kottke,Tilmann, Dick,Bernhard, Federov,Roman, Schlichting,Ilme, Deutzmann,Rainer, Hegemann,Peter
Phot photoreceptors make up an important protein family regulating biological processes in response to blue light. They contain two light, oxygen, and voltage sensitive (LOV) domains and a...
Fedorov,Roman, Schlichting,Ilme, Hartmann,Elisabeth, Domratcheva,Tatjana, Fuhrmann,Markus, Hegemann,Peter
Phot proteins (phototropins and homologs) are blue-light photoreceptors that control mechanical processes like phototropism, chloroplast relocation, or guard-cell opening in plants. Phot receptors...
Structure of Rab Escort Protein-1 in Complex with Rab Geranylgeranyltransferase (2003)
Pylypenko,Olena, Rak,Alexey, Reents,Reinhard, Niculae,Anca, Sidorovitch,Vadim, Cioaca,Maria-Daniela, ...
Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort...
Fedorov,Roman, Ghosh,Dipak K., Schlichting,Ilme
The crystal structure of the ternary cyanide complex of P450cam and camphor was determined to 1.8 Angstrom resolution and found to be identical with the structure of the active oxygen complex [I....
Fedorov,Roman, Ghosh,Dipak K., Schlichting,Ilme
The crystal structure of the ternary cyanide complex of P450cam and camphor was determined to 1.8 Å resolution and found to be identical with the structure of the active oxygen complex [I....
Fedorov,Roman, Schlichting,Ilme, Hartmann,Elisabeth, Domratcheva,Tatjana, Fuhrmann,Markus, Hegemann,Peter
Irreversible photoreduction of flavin in a mutated Phot-LOV1 domain (2003)
Kottke,Tilman, Dick,Bernhard, Fedorov,Roman, Schlichting,Ilme, Deutzmann,Rainer, Hegemann,Peter
Structure of rab escort protein-1 in complex with Rab geranylgeranyltransferase (2003)
Pylypenko,Olena, Rak,Alexey, Reents,Reinhard, Niculae,Anca, Sidorovitch,Vadim, Cioaca,Maria-Daniela, ...
Fedorov, Roman, Ghosh, Dipak K., Schlichting, Ilme
The crystal structure of the ternary cyanide complex of P450cam and camphor was determined to 1.8 Angstrom resolution and found to be identical with the structure of the active oxygen complex [I....
Fedorov, Roman, Ghosh, Dipak K., Schlichting, Ilme
The crystal structure of the ternary cyanide complex of P450cam and camphor was determined to 1.8 Å resolution and found to be identical with the structure of the active oxygen complex [I....
Structure of Rab Escort Protein-1 in Complex with Rab Geranylgeranyltransferase (2003)
Pylypenko, Olena, Rak, Alexey, Reents, Reinhard, Niculae, Anca, Sidorovitch, Vadim, Cioaca, Maria-Daniela, ...
Posttranslational geranylgeranylation of Rab GTPases is catalyzed by Rab geranylgeranyltransferase (RabGGTase), which consists of a catalytic alpha/beta heterodimer and an accessory Rab escort...
Fedorov, Roman, Schlichting, Ilme, Hartmann, Elisabeth, Domratcheva, Tatjana, Fuhrmann, Markus, Hegemann, Peter
Phot proteins (phototropins and homologs) are blue-light photoreceptors that control mechanical processes like phototropism, chloroplast relocation, or guard-cell opening in plants. Phot receptors...
Federov, Roman, Hartmann, Elisabeth, Ghosh, Dipak K., Schlichting, Ilme
The high level of amino acid conservation and structural similarity in the immediate vicinity of the substrate binding sites of the oxygenase domains of the nitric-oxide synthase (NOS) isoforms...
Irreversible Photoreduction of Flavin in a Mutated Phot-LOV1 Domain (2003)
Kottke, Tilmann, Dick, Bernhard, Federov, Roman, Schlichting, Ilme, Deutzmann, Rainer, Hegemann, Peter
Phot photoreceptors make up an important protein family regulating biological processes in response to blue light. They contain two light, oxygen, and voltage sensitive (LOV) domains and a...
Pylypenko, Olena, Vitali, Francesca, Zerbe, Katja, Robinson, John A., Schlichting, Ilme
Gene inactivation studies point to the involvement of OxyC in catalyzing the last oxidative phenol coupling reaction during glycopeptide antibiotic biosynthesis. Presently, the substrate and exact...
Fedorov, Roman, Schlichting, Ilme, Hartmann, Elisabeth, Domratcheva, Tatjana, Fuhrmann, Markus, Hegemann, Peter