Igor B. Dawid

Dishevelled and Wnt signaling: is the nucleus the final frontier? (2005)

Habas, Raymond, Dawid, Igor B

Abstract The phosphoprotein Dishevelled (Dsh) is an essential component of Wnt signaling pathways and transduces signals into three separate branches, the canonical, non-canonical and Ca 2+ pathways....

Organizing the Vertebrate Embryo—A Balance of Induction and Competence (2004)

Igor B. Dawid

The current understanding of organizer formation and neural induction in vertebrate embryos is discussed.

Transforming growth factor-$\beta 5$ expression during early development of Xenopus laevis (2000)

Kondaiah, Paturu, Taira, Masanori, Vempati, Uma Devi, Dawid, Igor B

Members of the transforming growth factor-\beta $(TGF-\beta)$ superfamily play various roles during development in both vertebrates and invertebrates. Two isoforms, $TGF-\beta 2$ and -$\beta 5$, have...

Transforming growth factor-$\beta 5$ expression during early development of Xenopus laevis (2000)

Kondaiah, Paturu, Taira, Masanori, Vempati, Uma Devi, Dawid, Igor B

Members of the transforming growth factor-\beta $(TGF-\beta)$ superfamily play various roles during development in both vertebrates and invertebrates. Two isoforms, $TGF-\beta 2$ and -$\beta 5$, have...

Cloning and molecular characterization of the trithorax locus of Drosophila melanogaster (1989)

Mozer, Brian A., Dawid, Igor B.

The trithorax (trx) locus of Drosophila melanogaster affects segment determination primarily in the thoracic region. Mutant flies show transformations of the third and, to a lesser extent, first...

Antagonistic role of vega1 and bozozok/dharma homeobox genes in organizer formation

Kawahara, Atsuo, Wilm, Thomas, Solnica-Krezel, Lilianna, Dawid, Igor B.

During zebrafish development, zygotic gene expression initiated at the midblastula transition converts maternal information on embryo polarity into a transcriptional read-out. Expression of a...

cyclops encodes a nodal-related factor involved in midline signaling

Rebagliati, Michael R., Toyama, Reiko, Haffter, Pascal, Dawid, Igor B.

Ventral structures in the central nervous system are patterned by signals emanating from the underlying mesoderm as well as originating within the neuroectoderm. Mutations in the zebrafish, Danio...

Radiation hybrid mapping of the zebrafish genome

Hukriede, Neil A., Joly, Lucille, Tsang, Michael, Miles, Jennifer, Tellis, Patricia, Epstein, Jonathan A., ...

The zebrafish is an excellent genetic system for the study of vertebrate development and disease. In an effort to provide a rapid and robust tool for zebrafish gene mapping, a panel of radiation...

Transcriptional regulation of the Xlim-1 gene by activin is mediated by an element in intron I

Rebbert, Martha L., Dawid, Igor B.

The Xlim-1 gene is activated in the late blastula stage of Xenopus embryogenesis in the mesoderm, and its RNA product becomes concentrated in the Spemann organizer at early gastrula stage. A major...

Regulation of dorsal fate in the neuraxis by Wnt-1 and Wnt-3a

Saint-Jeannet, Jean-Pierre, He, Xi, Varmus, Harold E., Dawid, Igor B.

Members of the Wnt family of signaling molecules are expressed differentially along the dorsal–ventral axis of the developing neural tube. Thus we asked whether Wnt factors are involved in...

Role of the iroquois3 homeobox gene in organizer formation

Kudoh, Tetsuhiro, Dawid, Igor B.

In zebrafish, the organizer is thought to consist of two regions, the yolk syncytial layer (YSL) and the shield. The dorsal YSL appears to send signals that affect formation of the shield in the...

Ssdp proteins interact with the LIM-domain-binding protein Ldb1 to regulate development

Chen, Lan, Segal, Daniel, Hukriede, Neil A., Podtelejnikov, Alexandre V., Bayarsaihan, Dashzeveg, Kennison, James A., ...

The LIM-domain-binding protein Ldb1 is a key factor in the assembly of transcriptional complexes involving LIM-homeodomain proteins and other transcription factors that regulate animal development....

Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation

Habas, Raymond, Dawid, Igor B., He, Xi

Wnt/Frizzled (Fz) signaling controls cell polarity/movements during vertebrate gastrulation via incompletely defined mechanisms. We demonstrated previously that Wnt/Fz activation of Rho, a GTPase and...

The Mitochondrial Ribosome of Xenopus laevis

Swanson, Ronald F., Dawid, Igor B.

A mitochondrial ribosome with an approximate sedimentation coefficient of 60 S has been isolated from the ovary of the South African clawed toad, Xenopus laevis. This ribosome is active in a...

The LN54 Radiation Hybrid Map of Zebrafish Expressed Sequences

Hukriede, Neil, Fisher, Dan, Epstein, Jonathan, Joly, Lucille, Tellis, Patricia, Zhou, Yi, ...

To increase the density of a gene map of the zebrafish, Danio rerio, we have placed 3119 expressed sequence tags (ESTs) and cDNA sequences on the LN54 radiation hybrid (RH) panel. The ESTs and genes...

Nucleotide sequences at the boundaries between gene and insertion regions in the rDNA of Drosophila melanogaster

Dawid, Igor B., Rebbert, Martha L.

Ribosomal RNA genes interrupted by type 1 insertions of 1 kb and 0.5 kb have been sequenced through the insertion region and compared with an uninterrupted gene. The 0.5 kb insertion is flanked by a...

Alternative splicing generates an isoform of the human Sef gene with altered subcellular localization and specificity

Preger, Ella, Ziv, Inbal, Shabtay, Ariel, Sher, Ifat, Tsang, Michael, Dawid, Igor B., ...

Receptor tyrosine kinases (RTKs) control a multitude of biological processes and are therefore subjected to multiple levels of regulation. Negative feedback is one of the mechanisms that provide an...

Interspecific Recombination of Mitochondrial DNA Molecules in Hybrid Somatic Cells

Horak, Ivan, Coon, Hayden G., Dawid, Igor B.

The mitochondrial DNA (mtDNA) in rodent-human hybrid somatic cells was studied in strains that contain nucleotide sequences from both parental mtDNAs. A test for linkage of rodent to human mtDNA was...

Organizing the Vertebrate Embryo—A Balance of Induction and Competence

Dawid, Igor B

The current understanding of organizer formation and neural induction in vertebrate embryos is discussed

Secondary Structure Maps of RNA: Processing of HeLa Ribosomal RNA

Wellauer, Peter K., Dawid, Igor B.

Ribosomal RNA precursors and mature 28S ribosomal RNA from HeLa cells display a highly reproducible secondary structure of hairpin loops after they are spread and examined in an electron microscope....

Sequences homologous to ribosomal insertions occur in the Drosophila genome outside the nucleolus organizer

Dawid, Igor B., Botchan, Peter

Many repeating units of Drosophila melanogaster rDNA contain a DNA sequence within the gene for 28S rRNA that does not code for rRNA. This sequence has been called the ribosomal insertion [Wellauer,...

Specialized and redundant roles of TBP and a vertebrate-specific TBP paralog in embryonic gene regulation in Xenopus

Jallow, Zainab, Jacobi, Ulrike G., Weeks, Daniel L., Dawid, Igor B., Veenstra, Gert Jan C.

The general transcription factor TATA-binding protein (TBP) is a key initiation factor involved in transcription by all three eukaryotic RNA polymerases. In addition, the related metazoan-specific...

Zebrafish GADD45β genes are involved in somite segmentation

Kawahara, Atsuo, Che, Yong-Suk, Hanaoka, Ryuki, Takeda, Hiroyuki, Dawid, Igor B.

Somites in vertebrates are periodic segmented structures that give rise to the vertebrae and muscles of body. Somites are generated from presomitic mesoderm (PSM), but it is not fully understood how...

Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis

Takahashi, Nobuhiro, Dawid, Igor B.

Zebrafish has become a favorite model organism not only in genetics and developmental biology, but also for the study of cancer, neuroscience and metabolism. However, strategies for reverse genetics...

Dishevelled and Wnt signaling: is the nucleus the final frontier?

Habas, Raymond, Dawid, Igor B

The phosphoprotein Dishevelled (Dsh) is an essential component of Wnt signaling pathways and transduces signals into three separate branches, the canonical, non-canonical and Ca2+ pathways. How Dsh...

Renaturation and Hybridization Studies of Mitochondrial DNA

Dawid, Igor B., Wolstenholme, David R.

The products of the renaturation reaction of mitochondrial DNA from oocytes of Xenopus laevis have been studied by electron microscopy and CsCl equilibrium density gradient centrifugation. The...

The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney

Hong, Sung-Kook, Haldin, Caroline E., Lawson, Nathan D., Weinstein, Brant M., Dawid, Igor B., Hukriede, Neil A.

Mutation of the gene encoding the Mediator component thyroid hormone receptor-associated protein (TRAP)230/MED12 affects the development of multiple systems in zebrafish embryogenesis. We isolated...

The Use of Hybrid Somatic Cells as an Approach to Mitochondrial Genetics in Animals

Dawid, Igor B., Horak, Ivan, Coon, Hayden G.

We have studied the fate of parental mitochondrial DNA (mtDNA) in hybrid somatic cells derived by Sendai virus-induced fusion of human cells and mouse or rat cells. Many hybrid cell strains were...

Antagonistic role of vega1 and bozozok/dharma homeobox genes in organizer formation

Kawahara, Atsuo, Wilm, Thomas, Solnica-Krezel, Lilianna, Dawid, Igor B.

During zebrafish development, zygotic gene expression initiated at the midblastula transition converts maternal information on embryo polarity into a transcriptional read-out. Expression of a...

cyclops encodes a nodal-related factor involved in midline signaling

Rebagliati, Michael R., Toyama, Reiko, Haffter, Pascal, Dawid, Igor B.

Ventral structures in the central nervous system are patterned by signals emanating from the underlying mesoderm as well as originating within the neuroectoderm. Mutations in the zebrafish, Danio...

Radiation hybrid mapping of the zebrafish genome

Hukriede, Neil A., Joly, Lucille, Tsang, Michael, Miles, Jennifer, Tellis, Patricia, Epstein, Jonathan A., ...

The zebrafish is an excellent genetic system for the study of vertebrate development and disease. In an effort to provide a rapid and robust tool for zebrafish gene mapping, a panel of radiation...

Transcriptional regulation of the Xlim-1 gene by activin is mediated by an element in intron I

Rebbert, Martha L., Dawid, Igor B.

The Xlim-1 gene is activated in the late blastula stage of Xenopus embryogenesis in the mesoderm, and its RNA product becomes concentrated in the Spemann organizer at early gastrula stage. A major...

Regulation of dorsal fate in the neuraxis by Wnt-1 and Wnt-3a

Saint-Jeannet, Jean-Pierre, He, Xi, Varmus, Harold E., Dawid, Igor B.

Members of the Wnt family of signaling molecules are expressed differentially along the dorsal–ventral axis of the developing neural tube. Thus we asked whether Wnt factors are involved in...

Role of the iroquois3 homeobox gene in organizer formation

Kudoh, Tetsuhiro, Dawid, Igor B.

In zebrafish, the organizer is thought to consist of two regions, the yolk syncytial layer (YSL) and the shield. The dorsal YSL appears to send signals that affect formation of the shield in the...

Ssdp proteins interact with the LIM-domain-binding protein Ldb1 to regulate development

Chen, Lan, Segal, Daniel, Hukriede, Neil A., Podtelejnikov, Alexandre V., Bayarsaihan, Dashzeveg, Kennison, James A., ...

The LIM-domain-binding protein Ldb1 is a key factor in the assembly of transcriptional complexes involving LIM-homeodomain proteins and other transcription factors that regulate animal development....

Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation

Habas, Raymond, Dawid, Igor B., He, Xi

Wnt/Frizzled (Fz) signaling controls cell polarity/movements during vertebrate gastrulation via incompletely defined mechanisms. We demonstrated previously that Wnt/Fz activation of Rho, a GTPase and...

The Mitochondrial Ribosome of Xenopus laevis

Swanson, Ronald F., Dawid, Igor B.

A mitochondrial ribosome with an approximate sedimentation coefficient of 60 S has been isolated from the ovary of the South African clawed toad, Xenopus laevis. This ribosome is active in a...

The LN54 Radiation Hybrid Map of Zebrafish Expressed Sequences

Hukriede, Neil, Fisher, Dan, Epstein, Jonathan, Joly, Lucille, Tellis, Patricia, Zhou, Yi, ...

To increase the density of a gene map of the zebrafish, Danio rerio, we have placed 3119 expressed sequence tags (ESTs) and cDNA sequences on the LN54 radiation hybrid (RH) panel. The ESTs and genes...

Nucleotide sequences at the boundaries between gene and insertion regions in the rDNA of Drosophila melanogaster

Dawid, Igor B., Rebbert, Martha L.

Ribosomal RNA genes interrupted by type 1 insertions of 1 kb and 0.5 kb have been sequenced through the insertion region and compared with an uninterrupted gene. The 0.5 kb insertion is flanked by a...

Alternative splicing generates an isoform of the human Sef gene with altered subcellular localization and specificity

Preger, Ella, Ziv, Inbal, Shabtay, Ariel, Sher, Ifat, Tsang, Michael, Dawid, Igor B., ...

Receptor tyrosine kinases (RTKs) control a multitude of biological processes and are therefore subjected to multiple levels of regulation. Negative feedback is one of the mechanisms that provide an...

Interspecific Recombination of Mitochondrial DNA Molecules in Hybrid Somatic Cells

Horak, Ivan, Coon, Hayden G., Dawid, Igor B.

The mitochondrial DNA (mtDNA) in rodent-human hybrid somatic cells was studied in strains that contain nucleotide sequences from both parental mtDNAs. A test for linkage of rodent to human mtDNA was...

Organizing the Vertebrate Embryo—A Balance of Induction and Competence

Dawid, Igor B

The current understanding of organizer formation and neural induction in vertebrate embryos is discussed

Secondary Structure Maps of RNA: Processing of HeLa Ribosomal RNA

Wellauer, Peter K., Dawid, Igor B.

Ribosomal RNA precursors and mature 28S ribosomal RNA from HeLa cells display a highly reproducible secondary structure of hairpin loops after they are spread and examined in an electron microscope....

Sequences homologous to ribosomal insertions occur in the Drosophila genome outside the nucleolus organizer

Dawid, Igor B., Botchan, Peter

Many repeating units of Drosophila melanogaster rDNA contain a DNA sequence within the gene for 28S rRNA that does not code for rRNA. This sequence has been called the ribosomal insertion [Wellauer,...

Specialized and redundant roles of TBP and a vertebrate-specific TBP paralog in embryonic gene regulation in Xenopus

Jallow, Zainab, Jacobi, Ulrike G., Weeks, Daniel L., Dawid, Igor B., Veenstra, Gert Jan C.

The general transcription factor TATA-binding protein (TBP) is a key initiation factor involved in transcription by all three eukaryotic RNA polymerases. In addition, the related metazoan-specific...

Zebrafish GADD45β genes are involved in somite segmentation

Kawahara, Atsuo, Che, Yong-Suk, Hanaoka, Ryuki, Takeda, Hiroyuki, Dawid, Igor B.

Somites in vertebrates are periodic segmented structures that give rise to the vertebrae and muscles of body. Somites are generated from presomitic mesoderm (PSM), but it is not fully understood how...

Dishevelled and Wnt signaling: is the nucleus the final frontier?

Habas, Raymond, Dawid, Igor B

The phosphoprotein Dishevelled (Dsh) is an essential component of Wnt signaling pathways and transduces signals into three separate branches, the canonical, non-canonical and Ca2+ pathways. How Dsh...

Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis

Takahashi, Nobuhiro, Dawid, Igor B.

Zebrafish has become a favorite model organism not only in genetics and developmental biology, but also for the study of cancer, neuroscience and metabolism. However, strategies for reverse genetics...

The Use of Hybrid Somatic Cells as an Approach to Mitochondrial Genetics in Animals

Dawid, Igor B., Horak, Ivan, Coon, Hayden G.

We have studied the fate of parental mitochondrial DNA (mtDNA) in hybrid somatic cells derived by Sendai virus-induced fusion of human cells and mouse or rat cells. Many hybrid cell strains were...

The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney

Hong, Sung-Kook, Haldin, Caroline E., Lawson, Nathan D., Weinstein, Brant M., Dawid, Igor B., Hukriede, Neil A.

Mutation of the gene encoding the Mediator component thyroid hormone receptor-associated protein (TRAP)230/MED12 affects the development of multiple systems in zebrafish embryogenesis. We isolated...

Renaturation and Hybridization Studies of Mitochondrial DNA

Dawid, Igor B., Wolstenholme, David R.

The products of the renaturation reaction of mitochondrial DNA from oocytes of Xenopus laevis have been studied by electron microscopy and CsCl equilibrium density gradient centrifugation. The...