Hubert Laude

Isolation from Cattle of a Prion Strain Distinct from That Causing Bovine Spongiform Encephalopathy (2006)

Vincent Béringue, Anna Bencsik, Annick Le Dur, Fabienne Reine, Thanh Lan Laï, Nathalie Chenais, ...

To date, bovine spongiform encephalopathy (BSE) and its human counterpart, variant Creutzfeldt-Jakob disease, have been associated with a single prion strain. This strain is characterised by a unique...

Transmissible Gastroenteritis Coronavirus Induces Programmed Cell Death in Infected Cells through a Caspase-Dependent Pathway

Eleouet, Jean-François, Chilmonczyk, Stefan, Besnardeau, Lydia, Laude, Hubert

In this report, we show that apoptosis (or programmed cell death) is induced in different cell lines infected with a coronavirus, the porcine transmissible gastroenteritis virus (TGEV). Kinetic...

Coronavirus Pseudoparticles Formed with Recombinant M and E Proteins Induce Alpha Interferon Synthesis by Leukocytes

Baudoux, Pierre, Carrat, Charles, Besnardeau, Lydia, Charley, Bernard, Laude, Hubert

Transmissible gastroenteritis virus (TGEV), an enteric coronavirus of swine, is a potent inducer of alpha interferon (IFN-α) both in vivo and in vitro. Incubation of peripheral blood mononuclear...

Successful Transmission of Three Mouse-Adapted Scrapie Strains to Murine Neuroblastoma Cell Lines Overexpressing Wild-Type Mouse Prion Protein

Nishida, Noriyuki, Harris, David A., Vilette, Didier, Laude, Hubert, Frobert, Yveline, Grassi, Jacques, ...

Propagation of the agents responsible for transmissible spongiform encephalopathies (TSEs) in cultured cells has been achieved for only a few cell lines. To establish efficient and versatile models...

The Membrane M Protein Carboxy Terminus Binds to Transmissible Gastroenteritis Coronavirus Core and Contributes to Core Stability

Escors, David, Ortego, Javier, Laude, Hubert, Enjuanes, Luis

The architecture of transmissible gastroenteritis coronavirus includes three different structural levels, the envelope, an internal core, and the nucleocapsid that is released when the core is...

Markedly Increased Susceptibility to Natural Sheep Scrapie of Transgenic Mice Expressing Ovine PrP

Vilotte, Jean-Luc, Soulier, Solange, Essalmani, Rachid, Stinnakre, Marie-George, Vaiman, Daniel, Lepourry, Laurence, ...

The susceptibility of sheep to scrapie is known to involve, as a major determinant, the nature of the prion protein (PrP) allele, with the VRQ allele conferring the highest susceptibility to the...

Organization of Two Transmissible Gastroenteritis Coronavirus Membrane Protein Topologies within the Virion and Core

Escors, David, Camafeita, Emilio, Ortego, Javier, Laude, Hubert, Enjuanes, Luis

The difference in membrane (M) protein compositions between the transmissible gastroenteritis coronavirus (TGEV) virion and the core has been studied. The TGEV M protein adopts two topologies in the...

Binding of Transmissible Gastroenteritis Coronavirus to Cell Surface Sialoglycoproteins

Schwegmann-Weßels, Christel, Zimmer, Gert, Laude, Hubert, Enjuanes, Luis, Herrler, Georg

The surface glycoprotein S of transmissible gastroenteritis virus (TGEV) has two binding activities. (i) Binding to porcine aminopeptidase N (pAPN) is essential for the initiation of infection. (ii)...

Generation of a Replication-Competent, Propagation-Deficient Virus Vector Based on the Transmissible Gastroenteritis Coronavirus Genome

Ortego, Javier, Escors, David, Laude, Hubert, Enjuanes, Luis

Replication-competent propagation-deficient virus vectors based on the transmissible gastroenteritis coronavirus (TGEV) genome that are deficient in the essential E gene have been developed by...

PrP Polymorphisms Tightly Control Sheep Prion Replication in Cultured Cells

Sabuncu, Elifsu, Petit, Stéphanie, Le Dur, Annick, Lan Lai, Thanh, Vilotte, Jean-Luc, Laude, Hubert, ...

Prion diseases are fatal neurodegenerative disorders of animals and humans that are characterized by the conversion of the host-encoded prion protein (PrP) to an abnormal isoform. In several species,...

Cultured Peripheral Neuroglial Cells Are Highly Permissive to Sheep Prion Infection

Archer, Fabienne, Bachelin, Corinne, Andreoletti, Olivier, Besnard, Nathalie, Perrot, Gregory, Langevin, Christelle, ...

Transmissible spongiform encephalopathies arise as a consequence of infection of the central nervous system (CNS) by prions. Spreading of the infectious agent through the peripheral nervous system...

Prion Infection of Epithelial Rov Cells Is a Polarized Event

Paquet, Sophie, Sabuncu, Elifsu, Delaunay, Jean-Louis, Laude, Hubert, Vilette, Didier

During prion infections, the cellular glycosylphosphatidylinositol-anchored glycoprotein PrP is converted into a conformational isoform. This abnormal conformer is thought to recruit and convert the...

Cells release prions in association with exosomes

Fevrier, Benoit, Vilette, Didier, Archer, Fabienne, Loew, Damarys, Faigle, Wolfgang, Vidal, Michel, ...

Prion diseases are infectious neurodegenerative disorders linked to the accumulation in the central nervous system of the abnormally folded prion protein (PrP) scrapie (PrPsc), which is thought to be...

Glycan-Controlled Epitopes of Prion Protein Include a Major Determinant of Susceptibility to Sheep Scrapie†

Moudjou, Mohammed, Treguer, Eric, Rezaei, Human, Sabuncu, Elifsu, Neuendorf, Erdi, Groschup, Martin H., ...

A key feature of prion encephalopathies is the accumulation of a misfolded form of the host glycoprotein PrP. Cell-free and cell culture studies have shown that the efficiency of conversion of PrP...

Prions can infect primary cultured neurons and astrocytes and promote neuronal cell death

Cronier, Sabrina, Laude, Hubert, Peyrin, Jean-Michel

Transmissible spongiform encephalopathies arise as a consequence of infection of the central nervous system by prions, where neurons and glial cells are regarded as primary targets. Neuronal loss and...

A newly identified type of scrapie agent can naturally infect sheep with resistant PrP genotypes

Le Dur, Annick, Béringue, Vincent, Andréoletti, Olivier, Reine, Fabienne, Laï, Thanh Lan, Baron, Thierry, ...

Scrapie in small ruminants belongs to transmissible spongiform encephalopathies (TSEs), or prion diseases, a family of fatal neurodegenerative disorders that affect humans and animals and can...

Isolation from Cattle of a Prion Strain Distinct from That Causing Bovine Spongiform Encephalopathy

Béringue, Vincent, Bencsik, Anna, Le Dur, Annick, Reine, Fabienne, Laï, Thanh Lan, Chenais, Nathalie, ...

To date, bovine spongiform encephalopathy (BSE) and its human counterpart, variant Creutzfeldt-Jakob disease, have been associated with a single prion strain. This strain is characterised by a unique...

Transmissible Gastroenteritis Coronavirus Induces Programmed Cell Death in Infected Cells through a Caspase-Dependent Pathway

Eleouet, Jean-François, Chilmonczyk, Stefan, Besnardeau, Lydia, Laude, Hubert

In this report, we show that apoptosis (or programmed cell death) is induced in different cell lines infected with a coronavirus, the porcine transmissible gastroenteritis virus (TGEV). Kinetic...

Coronavirus Pseudoparticles Formed with Recombinant M and E Proteins Induce Alpha Interferon Synthesis by Leukocytes

Baudoux, Pierre, Carrat, Charles, Besnardeau, Lydia, Charley, Bernard, Laude, Hubert

Transmissible gastroenteritis virus (TGEV), an enteric coronavirus of swine, is a potent inducer of alpha interferon (IFN-α) both in vivo and in vitro. Incubation of peripheral blood mononuclear...

Successful Transmission of Three Mouse-Adapted Scrapie Strains to Murine Neuroblastoma Cell Lines Overexpressing Wild-Type Mouse Prion Protein

Nishida, Noriyuki, Harris, David A., Vilette, Didier, Laude, Hubert, Frobert, Yveline, Grassi, Jacques, ...

Propagation of the agents responsible for transmissible spongiform encephalopathies (TSEs) in cultured cells has been achieved for only a few cell lines. To establish efficient and versatile models...

The Membrane M Protein Carboxy Terminus Binds to Transmissible Gastroenteritis Coronavirus Core and Contributes to Core Stability

Escors, David, Ortego, Javier, Laude, Hubert, Enjuanes, Luis

The architecture of transmissible gastroenteritis coronavirus includes three different structural levels, the envelope, an internal core, and the nucleocapsid that is released when the core is...

Markedly Increased Susceptibility to Natural Sheep Scrapie of Transgenic Mice Expressing Ovine PrP

Vilotte, Jean-Luc, Soulier, Solange, Essalmani, Rachid, Stinnakre, Marie-George, Vaiman, Daniel, Lepourry, Laurence, ...

The susceptibility of sheep to scrapie is known to involve, as a major determinant, the nature of the prion protein (PrP) allele, with the VRQ allele conferring the highest susceptibility to the...

Organization of Two Transmissible Gastroenteritis Coronavirus Membrane Protein Topologies within the Virion and Core

Escors, David, Camafeita, Emilio, Ortego, Javier, Laude, Hubert, Enjuanes, Luis

The difference in membrane (M) protein compositions between the transmissible gastroenteritis coronavirus (TGEV) virion and the core has been studied. The TGEV M protein adopts two topologies in the...

Binding of Transmissible Gastroenteritis Coronavirus to Cell Surface Sialoglycoproteins

Schwegmann-Weßels, Christel, Zimmer, Gert, Laude, Hubert, Enjuanes, Luis, Herrler, Georg

The surface glycoprotein S of transmissible gastroenteritis virus (TGEV) has two binding activities. (i) Binding to porcine aminopeptidase N (pAPN) is essential for the initiation of infection. (ii)...

Generation of a Replication-Competent, Propagation-Deficient Virus Vector Based on the Transmissible Gastroenteritis Coronavirus Genome

Ortego, Javier, Escors, David, Laude, Hubert, Enjuanes, Luis

Replication-competent propagation-deficient virus vectors based on the transmissible gastroenteritis coronavirus (TGEV) genome that are deficient in the essential E gene have been developed by...

PrP Polymorphisms Tightly Control Sheep Prion Replication in Cultured Cells

Sabuncu, Elifsu, Petit, Stéphanie, Le Dur, Annick, Lan Lai, Thanh, Vilotte, Jean-Luc, Laude, Hubert, ...

Prion diseases are fatal neurodegenerative disorders of animals and humans that are characterized by the conversion of the host-encoded prion protein (PrP) to an abnormal isoform. In several species,...

Cultured Peripheral Neuroglial Cells Are Highly Permissive to Sheep Prion Infection

Archer, Fabienne, Bachelin, Corinne, Andreoletti, Olivier, Besnard, Nathalie, Perrot, Gregory, Langevin, Christelle, ...

Transmissible spongiform encephalopathies arise as a consequence of infection of the central nervous system (CNS) by prions. Spreading of the infectious agent through the peripheral nervous system...

Prion Infection of Epithelial Rov Cells Is a Polarized Event

Paquet, Sophie, Sabuncu, Elifsu, Delaunay, Jean-Louis, Laude, Hubert, Vilette, Didier

During prion infections, the cellular glycosylphosphatidylinositol-anchored glycoprotein PrP is converted into a conformational isoform. This abnormal conformer is thought to recruit and convert the...

Cells release prions in association with exosomes

Fevrier, Benoit, Vilette, Didier, Archer, Fabienne, Loew, Damarys, Faigle, Wolfgang, Vidal, Michel, ...

Prion diseases are infectious neurodegenerative disorders linked to the accumulation in the central nervous system of the abnormally folded prion protein (PrP) scrapie (PrPsc), which is thought to be...

Glycan-Controlled Epitopes of Prion Protein Include a Major Determinant of Susceptibility to Sheep Scrapie†

Moudjou, Mohammed, Treguer, Eric, Rezaei, Human, Sabuncu, Elifsu, Neuendorf, Erdi, Groschup, Martin H., ...

A key feature of prion encephalopathies is the accumulation of a misfolded form of the host glycoprotein PrP. Cell-free and cell culture studies have shown that the efficiency of conversion of PrP...

Prions can infect primary cultured neurons and astrocytes and promote neuronal cell death

Cronier, Sabrina, Laude, Hubert, Peyrin, Jean-Michel

Transmissible spongiform encephalopathies arise as a consequence of infection of the central nervous system by prions, where neurons and glial cells are regarded as primary targets. Neuronal loss and...

A newly identified type of scrapie agent can naturally infect sheep with resistant PrP genotypes

Le Dur, Annick, Béringue, Vincent, Andréoletti, Olivier, Reine, Fabienne, Laï, Thanh Lan, Baron, Thierry, ...

Scrapie in small ruminants belongs to transmissible spongiform encephalopathies (TSEs), or prion diseases, a family of fatal neurodegenerative disorders that affect humans and animals and can...

Isolation from Cattle of a Prion Strain Distinct from That Causing Bovine Spongiform Encephalopathy

Béringue, Vincent, Bencsik, Anna, Le Dur, Annick, Reine, Fabienne, Laï, Thanh Lan, Chenais, Nathalie, ...

To date, bovine spongiform encephalopathy (BSE) and its human counterpart, variant Creutzfeldt-Jakob disease, have been associated with a single prion strain. This strain is characterised by a unique...

PrPc Does Not Mediate Internalization of PrPSc but Is Required at an Early Stage for De Novo Prion Infection of Rov Cells▿

Paquet, Sophie, Daude, Nathalie, Courageot, Marie-Pierre, Chapuis, Jérôme, Laude, Hubert, Vilette, Didier

We have studied the interactions of exogenous prions with an epithelial cell line inducibly expressing PrPc protein and permissive to infection by a sheep scrapie agent. We demonstrate that abnormal...

Prion Strain- and Species-Dependent Effects of Antiprion Molecules in Primary Neuronal Cultures▿

Cronier, Sabrina, Beringue, Vincent, Bellon, Anne, Peyrin, Jean-Michel, Laude, Hubert

Transmissible spongiform encephalopathies (TSE) arise as a consequence of infection of the central nervous system by prions and are incurable. To date, most antiprion compounds identified by in vitro...