Hideaki Usuda

Psr1, a nuclear localized protein that regulates phosphorus metabolism in Chlamydomonas

Wykoff, Dennis D., Grossman, Arthur R., Weeks, Donald P., Usuda, Hideaki, Shimogawara, Kosuke

Understanding the ways in which phosphorus metabolism is regulated in photosynthetic eukaryotes is critical for optimizing crop productivity and managing aquatic ecosystems in which phosphorus can be...

Chlamydomonas reinhardtii Mutants Abnormal in Their Responses to Phosphorus Deprivation1

Shimogawara, Kosuke, Wykoff, Dennis D., Usuda, Hideaki, Grossman, Arthur R.

P-starved plants scavenge inorganic phosphate (Pi) by developing elevated rates of Pi uptake, synthesizing extracellular phosphatases, and secreting organic acids. To elucidate mechanisms controlling...

Inhibition of Photosynthetic Carbon Metabolism in Isolated Chloroplasts by Iodoacetol Phosphate 12

Usuda, Hideaki, Edwards, Gerald E.

Carbon dioxide-dependent and 3-phosphoglycerate (PGA)-dependent O2 evolution by isolated chloroplasts of wheat is inhibited by micromolar levels of iodoacetol phosphate (IAP). Loss of the activity is...

Influence of Varying CO2 and Orthophosphate Concentrations on Rates of Photosynthesis, and Synthesis of Glycolate and Dihydroxyacetone Phosphate by Wheat Chloroplasts 1

Usuda, Hideaki, Edwards, Gerald E.

Intact chloroplasts of wheat (Triticum aestivum) were isolated from mesophyll protoplasts. With decreasing concentrations of bicarbonate from 10 to 0.3 millimolar (pH 8.0), the optimal concentration...

Light-Induced Conversion of Nicotinamide Adenine Dinucleotide to Nicotinamide Adenine Dinucleotide Phosphate in Higher Plant Leaves 1

Muto, Shoshi, Miyachi, Shigetoh, Usuda, Hideaki, Edwards, Gerald E., Bassham, James A.

Light-induced conversion of NAD to NADP was investigated in higher plants. Upon illumination, conversion of NAD to NADP was observed in intact leaves of wheat and pea following incubation in the...

Localization of Glycerate Kinase and Some Enzymes for Sucrose Synthesis in C3 and C4 Plants 1

Usuda, Hideaki, Edwards, Gerald E.

The localization of some key enzymes leading to sucrose synthesis in photosynthetic tissue of C3 and C4 species was investigated. These included UDP-glucose (UDPG) pyrophosphorylase, sucrose...

Nonaqueous Purification of Maize Mesophyll Chloroplasts 1

Usuda, Hideaki

A nonaqueous fractionation method to obtain highly purified mesophyll chloroplasts from lyophilized leaves of Zea mays L. is described. The levels of several metabolites including pyruvate were...

Adenine Nucleotide Levels, the Redox State of the NADP System, and Assimilatory Force in Nonaqueously Purified Mesophyll Chloroplasts from Maize Leaves under Different Light Intensities 1

Usuda, Hideaki

Recently, a nonaqueous fractionation method of obtaining highly purified mesophyll chloroplasts from maize leaves was established. This method is now used to determine adenine nucleotide levels, the...

Changes in Levels of Intermediates of the C4 Cycle and Reductive Pentose Phosphate Pathway under Various Concentrations of CO2 in Maize Leaves 1

Usuda, Hideaki

The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in an attached leaf of maize (Zea mays L) were measured over a range of intercellular...

Diurnal Changes in Maize Leaf Photosynthesis 1: I. Carbon Exchange Rate, Assimilate Export Rate, and Enzyme Activities

Kalt-Torres, Willy, Kerr, Phillip S., Usuda, Hideaki, Huber, Steven C.

Diurnal changes in photosynthetic parameters and enzyme activities were characterized in greenhouse grown maize plants (Zea mays L. cv Pioneer 3184). Rates of net photosynthesis and assimilate export...

Diurnal Changes in Maize Leaf Photosynthesis 1: II. Levels of Metabolic Intermediates of Sucrose Synthesis and the Regulatory Metabolite Fructose 2,6-Bisphosphate

Usuda, Hideaki, Kalt-Torres, Willy, Kerr, Phillip S., Huber, Steven C.

Diurnal changes in the regulatory metabolite, fructose-2,6-bisphosphate (F26BP), and key metabolic intermediates of sucrose biosynthesis were studied in maize (Zea mays L. cv Pioneer 3184) during a...

Changes in Levels of Intermediates of the C4 Cycle and Reductive Pentose Phosphate Pathway under Various Light Intensities in Maize Leaves 1

Usuda, Hideaki

The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in attached leaves of maize (Zea mays L.) were measured over a range of light intensity...

Activation of NADP-Malate Dehydrogenase, Pyruvate,Pi Dikinase, and Fructose 1,6-Bisphosphatase in Relation to Photosynthetic Rate in Maize 1

Usuda, Hideaki, Ku, Maurice S. B., Edwards, Gerald E.

The activity and extent of light activation of three photosynthetic enzymes, pyruvate,Pi dikinase, NADP-malate dehydrogenase (NADP-MDH), and fructose 1,6-bisphosphatase (FBPase), were examined in...

Changes in Levels of Intermediates of the C4 Cycle and Reductive Pentose Phosphate Pathway during Induction of Photosynthesis in Maize Leaves 1

Usuda, Hideaki

Changes in the level of metabolites of the C4 cycle and reductive pentose phosphate (RPP) pathway were measured simultaneously with induction of photosynthesis in maize (Zea mays L.) to evaluate what...

Influence of Nitrate and Ammonia on Photosynthetic Characteristics and Leaf Anatomy of Moricandia arvensis1

Winter, Klaus, Usuda, Hideaki, Tsuzuki, Mikio, Schmitt, Mark, Edwards, Gerald E., Thomas, Richard J., ...

The leaf anatomy and certain photosynthetic properties of nitrate- and ammonia-grown plants of Moricandia arvensis (L.) DC., a species previously reported to be a C3-C4 intermediate, were...

Phosphate Deficiency in Maize 1: III. Changes in Enzyme Activities during the Course of Phosphate Deprivation

Usuda, Hideaki, Shimogawara, Kousuke

The effects of low concentrations of phosphate (low-P) on soluble protein content, the activities of 12 different enzymes, and the rates of photosynthesis and respiration on the basis of leaf area...

Psr1, a nuclear localized protein that regulates phosphorus metabolism in Chlamydomonas

Wykoff, Dennis D., Grossman, Arthur R., Weeks, Donald P., Usuda, Hideaki, Shimogawara, Kosuke

Understanding the ways in which phosphorus metabolism is regulated in photosynthetic eukaryotes is critical for optimizing crop productivity and managing aquatic ecosystems in which phosphorus can be...

Chlamydomonas reinhardtii Mutants Abnormal in Their Responses to Phosphorus Deprivation1

Shimogawara, Kosuke, Wykoff, Dennis D., Usuda, Hideaki, Grossman, Arthur R.

P-starved plants scavenge inorganic phosphate (Pi) by developing elevated rates of Pi uptake, synthesizing extracellular phosphatases, and secreting organic acids. To elucidate mechanisms controlling...

Inhibition of Photosynthetic Carbon Metabolism in Isolated Chloroplasts by Iodoacetol Phosphate 12

Usuda, Hideaki, Edwards, Gerald E.

Carbon dioxide-dependent and 3-phosphoglycerate (PGA)-dependent O2 evolution by isolated chloroplasts of wheat is inhibited by micromolar levels of iodoacetol phosphate (IAP). Loss of the activity is...

Influence of Varying CO2 and Orthophosphate Concentrations on Rates of Photosynthesis, and Synthesis of Glycolate and Dihydroxyacetone Phosphate by Wheat Chloroplasts 1

Usuda, Hideaki, Edwards, Gerald E.

Intact chloroplasts of wheat (Triticum aestivum) were isolated from mesophyll protoplasts. With decreasing concentrations of bicarbonate from 10 to 0.3 millimolar (pH 8.0), the optimal concentration...

Light-Induced Conversion of Nicotinamide Adenine Dinucleotide to Nicotinamide Adenine Dinucleotide Phosphate in Higher Plant Leaves 1

Muto, Shoshi, Miyachi, Shigetoh, Usuda, Hideaki, Edwards, Gerald E., Bassham, James A.

Light-induced conversion of NAD to NADP was investigated in higher plants. Upon illumination, conversion of NAD to NADP was observed in intact leaves of wheat and pea following incubation in the...

Localization of Glycerate Kinase and Some Enzymes for Sucrose Synthesis in C3 and C4 Plants 1

Usuda, Hideaki, Edwards, Gerald E.

The localization of some key enzymes leading to sucrose synthesis in photosynthetic tissue of C3 and C4 species was investigated. These included UDP-glucose (UDPG) pyrophosphorylase, sucrose...

Nonaqueous Purification of Maize Mesophyll Chloroplasts 1

Usuda, Hideaki

A nonaqueous fractionation method to obtain highly purified mesophyll chloroplasts from lyophilized leaves of Zea mays L. is described. The levels of several metabolites including pyruvate were...

Adenine Nucleotide Levels, the Redox State of the NADP System, and Assimilatory Force in Nonaqueously Purified Mesophyll Chloroplasts from Maize Leaves under Different Light Intensities 1

Usuda, Hideaki

Recently, a nonaqueous fractionation method of obtaining highly purified mesophyll chloroplasts from maize leaves was established. This method is now used to determine adenine nucleotide levels, the...

Changes in Levels of Intermediates of the C4 Cycle and Reductive Pentose Phosphate Pathway under Various Concentrations of CO2 in Maize Leaves 1

Usuda, Hideaki

The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in an attached leaf of maize (Zea mays L) were measured over a range of intercellular...

Diurnal Changes in Maize Leaf Photosynthesis 1: I. Carbon Exchange Rate, Assimilate Export Rate, and Enzyme Activities

Kalt-Torres, Willy, Kerr, Phillip S., Usuda, Hideaki, Huber, Steven C.

Diurnal changes in photosynthetic parameters and enzyme activities were characterized in greenhouse grown maize plants (Zea mays L. cv Pioneer 3184). Rates of net photosynthesis and assimilate export...

Diurnal Changes in Maize Leaf Photosynthesis 1: II. Levels of Metabolic Intermediates of Sucrose Synthesis and the Regulatory Metabolite Fructose 2,6-Bisphosphate

Usuda, Hideaki, Kalt-Torres, Willy, Kerr, Phillip S., Huber, Steven C.

Diurnal changes in the regulatory metabolite, fructose-2,6-bisphosphate (F26BP), and key metabolic intermediates of sucrose biosynthesis were studied in maize (Zea mays L. cv Pioneer 3184) during a...

Changes in Levels of Intermediates of the C4 Cycle and Reductive Pentose Phosphate Pathway under Various Light Intensities in Maize Leaves 1

Usuda, Hideaki

The rate of CO2 assimilation and levels of metabolites of the C4 cycle and reductive pentose phosphate pathway in attached leaves of maize (Zea mays L.) were measured over a range of light intensity...

Activation of NADP-Malate Dehydrogenase, Pyruvate,Pi Dikinase, and Fructose 1,6-Bisphosphatase in Relation to Photosynthetic Rate in Maize 1

Usuda, Hideaki, Ku, Maurice S. B., Edwards, Gerald E.

The activity and extent of light activation of three photosynthetic enzymes, pyruvate,Pi dikinase, NADP-malate dehydrogenase (NADP-MDH), and fructose 1,6-bisphosphatase (FBPase), were examined in...

Changes in Levels of Intermediates of the C4 Cycle and Reductive Pentose Phosphate Pathway during Induction of Photosynthesis in Maize Leaves 1

Usuda, Hideaki

Changes in the level of metabolites of the C4 cycle and reductive pentose phosphate (RPP) pathway were measured simultaneously with induction of photosynthesis in maize (Zea mays L.) to evaluate what...

Influence of Nitrate and Ammonia on Photosynthetic Characteristics and Leaf Anatomy of Moricandia arvensis1

Winter, Klaus, Usuda, Hideaki, Tsuzuki, Mikio, Schmitt, Mark, Edwards, Gerald E., Thomas, Richard J., ...

The leaf anatomy and certain photosynthetic properties of nitrate- and ammonia-grown plants of Moricandia arvensis (L.) DC., a species previously reported to be a C3-C4 intermediate, were...

Phosphate Deficiency in Maize 1: III. Changes in Enzyme Activities during the Course of Phosphate Deprivation

Usuda, Hideaki, Shimogawara, Kousuke

The effects of low concentrations of phosphate (low-P) on soluble protein content, the activities of 12 different enzymes, and the rates of photosynthesis and respiration on the basis of leaf area...