Hidde Ploegh

Covalent modification of the active site threonine of proteasomal β subunits and the Escherichia coli homolog HslV by a new class of inhibitors

Bogyo, Matthew, McMaster, John S., Gaczynska, Maria, Tortorella, Domenico, Goldberg, Alfred L., Ploegh, Hidde

The proteasome is a multicatalytic protease complex that plays a key role in diverse cellular functions. The peptide vinyl sulfone, carboxybenzyl-leucyl-leucyl-leucine vinyl sulfone (Z-L3VS)...

Major histocompatibility complex (MHC) class I KbDb −/− deficient mice possess functional CD8+ T cells and natural killer cells

Vugmeyster, Yulia, Glas, Rickard, Pérarnau, Béatrice, Lemonnier, François A., Eisen, Herman, Ploegh, Hidde

We obtained mice deficient for major histocompatibility complex (MHC) molecules encoded by the H-2K and H-2D genes. H-2 KbDb −/− mice express no detectable classical MHC class I-region associated...

Functionality of major histocompatibility complex class II molecules in mice doubly deficient for invariant chain and H-2M complexes

Tourne, Sylvie, Miyazaki, Toru, Wolf, Paula, Ploegh, Hidde, Benoist, Christophe, Mathis, Diane

By combining two previously generated null mutations, Ii° and M°, we produced mice lacking the invariant chain and H-2M complexes, both required for normal cell-surface expression of major...

Inhibition of Major Histocompatibility Complex Class I Antigen Presentation in Pig and Primate Cells by Herpes Simplex Virus Type 1 and 2 ICP47

Jugovic, Pieter, Hill, Ann M., Tomazin, Roman, Ploegh, Hidde, Johnson, David C.

Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) express an immediate-early protein, ICP47, that effectively inhibits the human transporter associated with antigen presentation (TAP), blocking...

Varicella-Zoster Virus Retains Major Histocompatibility Complex Class I Proteins in the Golgi Compartment of Infected Cells

Abendroth, Allison, Lin, Ines, Slobedman, Barry, Ploegh, Hidde, Arvin, Ann M.

We sought to examine the effects of varicella-zoster virus (VZV) infection on the expression of major histocompatibility complex class I (MHC I) molecules by human fibroblasts and T lymphocytes. By...

Structural and Functional Analysis of Human Cytomegalovirus US3 Protein

Misaghi, Shahram, Sun, Zhen-Yu J., Stern, Patrick, Gaudet, Rachelle, Wagner, Gerhard, Ploegh, Hidde

Human cytomegalovirus (HCMV) unique short region 3 (US3) protein, a type I membrane protein, prevents maturation of class I major histocompatibility complex (MHC) molecules by retaining them in the...

The SUMO-Specific Protease SENP5 Is Required for Cell Division

Di Bacco, Alessandra, Ouyang, Jian, Lee, Hsiang-Ying, Catic, Andre, Ploegh, Hidde, Gill, Grace

Posttranslational modification of substrates by the small ubiquitin-like modifier, SUMO, regulates diverse biological processes, including transcription, DNA repair, nucleocytoplasmic trafficking,...

Covalent modification of the active site threonine of proteasomal β subunits and the Escherichia coli homolog HslV by a new class of inhibitors

Bogyo, Matthew, McMaster, John S., Gaczynska, Maria, Tortorella, Domenico, Goldberg, Alfred L., Ploegh, Hidde

The proteasome is a multicatalytic protease complex that plays a key role in diverse cellular functions. The peptide vinyl sulfone, carboxybenzyl-leucyl-leucyl-leucine vinyl sulfone (Z-L3VS)...

Major histocompatibility complex (MHC) class I KbDb −/− deficient mice possess functional CD8+ T cells and natural killer cells

Vugmeyster, Yulia, Glas, Rickard, Pérarnau, Béatrice, Lemonnier, François A., Eisen, Herman, Ploegh, Hidde

We obtained mice deficient for major histocompatibility complex (MHC) molecules encoded by the H-2K and H-2D genes. H-2 KbDb −/− mice express no detectable classical MHC class I-region associated...

Functionality of major histocompatibility complex class II molecules in mice doubly deficient for invariant chain and H-2M complexes

Tourne, Sylvie, Miyazaki, Toru, Wolf, Paula, Ploegh, Hidde, Benoist, Christophe, Mathis, Diane

By combining two previously generated null mutations, Ii° and M°, we produced mice lacking the invariant chain and H-2M complexes, both required for normal cell-surface expression of major...

Inhibition of Major Histocompatibility Complex Class I Antigen Presentation in Pig and Primate Cells by Herpes Simplex Virus Type 1 and 2 ICP47

Jugovic, Pieter, Hill, Ann M., Tomazin, Roman, Ploegh, Hidde, Johnson, David C.

Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) express an immediate-early protein, ICP47, that effectively inhibits the human transporter associated with antigen presentation (TAP), blocking...

Varicella-Zoster Virus Retains Major Histocompatibility Complex Class I Proteins in the Golgi Compartment of Infected Cells

Abendroth, Allison, Lin, Ines, Slobedman, Barry, Ploegh, Hidde, Arvin, Ann M.

We sought to examine the effects of varicella-zoster virus (VZV) infection on the expression of major histocompatibility complex class I (MHC I) molecules by human fibroblasts and T lymphocytes. By...

Structural and Functional Analysis of Human Cytomegalovirus US3 Protein

Misaghi, Shahram, Sun, Zhen-Yu J., Stern, Patrick, Gaudet, Rachelle, Wagner, Gerhard, Ploegh, Hidde

Human cytomegalovirus (HCMV) unique short region 3 (US3) protein, a type I membrane protein, prevents maturation of class I major histocompatibility complex (MHC) molecules by retaining them in the...

The SUMO-Specific Protease SENP5 Is Required for Cell Division

Di Bacco, Alessandra, Ouyang, Jian, Lee, Hsiang-Ying, Catic, Andre, Ploegh, Hidde, Gill, Grace

Posttranslational modification of substrates by the small ubiquitin-like modifier, SUMO, regulates diverse biological processes, including transcription, DNA repair, nucleocytoplasmic trafficking,...

Loss of Aire-dependent thymic expression of a peripheral tissue antigen renders it a target of autoimmunity

Gavanescu, Irina, Kessler, Benedikt, Ploegh, Hidde, Benoist, Christophe, Mathis, Diane

Both humans and mice with a mutation in the autoimmune regulator (aire) gene develop multiorgan autoimmune disease. Aire was shown to exert its critical function in medullary epithelial cells of the...

Qa-2–Dependent Selection of Cd8α/α T Cell Receptor α/β+ Cells in Murine Intestinal Intraepithelial Lymphocytes

Das, Gobardhan, Gould, Dina S., Augustine, Mathew M., Fragoso, Gladis, Scitto, Edda, Stroynowski, Iwona, ...

Murine intestinal intraepithelial lymphocytes (iIELs) are made up of a heterogeneous mix of T cells with unique phenotypes. Whereas CD8+ T cells in peripheral lymphoid organs use CD8α/β and are...

Cytokines Regulate Proteolysis in Major Histocompatibility Complex Class II–Dependent Antigen Presentation by Dendritic Cells

Fiebiger, Edda, Meraner, Paul, Weber, Ekkehard, Fang, I-Fei, Stingl, Georg, Ploegh, Hidde, ...

Endo/lysosomal proteases control two key events in antigen (Ag) presentation: the degradation of protein Ag and the generation of peptide-receptive major histocompatibility complex (MHC) class II...

A herpesvirus ubiquitin-specific protease is critical for efficient T cell lymphoma formation

Jarosinski, Keith, Kattenhorn, Lisa, Kaufer, Benedikt, Ploegh, Hidde, Osterrieder, Nikolaus

The herpesvirus ubiquitin-specific protease (USP) family, whose founding member was discovered as a protease domain embedded in the large tegument protein of herpes simplex virus 1 (HSV-1), is...