Herbert Tschochner

Functional architecture of RNA polymerase I (2007)

Kuhn, Claus-D., Geiger, Sebastian R., Baumli, Sonja, Gartmann, Marco, Gerber, Jochen, Jennebach, Stefan, ...

Synthesis of ribosomal RNA (rRNA) by RNA polymerase (Pol) I is the first step in ribosome biogenesis and a regulatory switch in eukaryotic cell growth. Here we report the 12 Å cryo-electron...

A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3′ end

Tschochner, Herbert

A novel RNase activity was identified in a yeast RNA polymerase I (pol I) in vitro transcription system. Transcript cleavage occurred at the 3′ end and was dependent on the presence of ternary pol...

Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I

Fath, Stephan, Milkereit, Philipp, Peyroche, Gerald, Riva, Michel, Carles, Christophe, Tschochner, Herbert

Regulation of rDNA transcription depends on the formation and dissociation of a functional complex between RNA polymerase I (pol I) and transcription initiation factor Rrn3p. We analyzed whether...

Localization of the yeast RNA polymerase I-specific subunits

Bischler, Nicolas, Brino, Laurent, Carles, Christophe, Riva, Michel, Tschochner, Herbert, Mallouh, Véronique, ...

The spatial distribution of four subunits specifically associated to the yeast DNA-dependent RNA polymerase I (RNA pol I) was studied by electron microscopy. A structural model of the native enzyme...

SURVEY AND SUMMARY: The pre-ribosomal network

Milkereit, Philipp, Kühn, Holger, Gas, Nicole, Tschochner, Herbert

Recent achievements in yeast functional proteomics have significantly advanced our knowledge about ribosome biogenesis. Here, we present a program developed to integrate data from various proteome...

The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3

Peyroche, Gérald, Milkereit, Philipp, Bischler, Nicolas, Tschochner, Herbert, Schultz, Patrick, Sentenac, André, ...

RNA polymerase I (Pol I) is dedicated to transcription of the large ribosomal DNA (rDNA). The mechanism of Pol I recruitment onto rDNA promoters is poorly understood. Here we present evidence that...

A novel RNA polymerase I-dependent RNase activity that shortens nascent transcripts from the 3′ end

Tschochner, Herbert

A novel RNase activity was identified in a yeast RNA polymerase I (pol I) in vitro transcription system. Transcript cleavage occurred at the 3′ end and was dependent on the presence of ternary pol...

Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I

Fath, Stephan, Milkereit, Philipp, Peyroche, Gerald, Riva, Michel, Carles, Christophe, Tschochner, Herbert

Regulation of rDNA transcription depends on the formation and dissociation of a functional complex between RNA polymerase I (pol I) and transcription initiation factor Rrn3p. We analyzed whether...

Localization of the yeast RNA polymerase I-specific subunits

Bischler, Nicolas, Brino, Laurent, Carles, Christophe, Riva, Michel, Tschochner, Herbert, Mallouh, Véronique, ...

The spatial distribution of four subunits specifically associated to the yeast DNA-dependent RNA polymerase I (RNA pol I) was studied by electron microscopy. A structural model of the native enzyme...

SURVEY AND SUMMARY: The pre-ribosomal network

Milkereit, Philipp, Kühn, Holger, Gas, Nicole, Tschochner, Herbert

Recent achievements in yeast functional proteomics have significantly advanced our knowledge about ribosome biogenesis. Here, we present a program developed to integrate data from various proteome...

The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3

Peyroche, Gérald, Milkereit, Philipp, Bischler, Nicolas, Tschochner, Herbert, Schultz, Patrick, Sentenac, André, ...

RNA polymerase I (Pol I) is dedicated to transcription of the large ribosomal DNA (rDNA). The mechanism of Pol I recruitment onto rDNA promoters is poorly understood. Here we present evidence that...

Site specific phosphorylation of yeast RNA polymerase I

Gerber, Jochen, Reiter, Alarich, Steinbauer, Robert, Jakob, Steffen, Kuhn, Claus-Dieter, Cramer, Patrick, ...

All nuclear RNA polymerases are phosphoprotein complexes. Yeast RNA polymerase I (Pol I) contains approximately 15 phosphate groups, distributed to 5 of the 14 subunits. Information about the...

Association of Yeast RNA Polymerase I with a Nucleolar Substructure Active in Rrna Synthesis and Processing

Fath, Stephan, Milkereit, Philipp, Podtelejnikov, Alexandre V., Bischler, Nicolas, Schultz, Patrick, Bier, Mirko, ...

A novel ribonucleoprotein complex enriched in nucleolar proteins was purified from yeast extracts and constituents were identified by mass spectrometry. When isolated from rapidly growing cells, the...