Hai-Meng Zhou

Axin stimulates p53 functions by activation of HIPK2 kinase through multimeric complex formation

Rui, Yanning, Xu, Zhen, Lin, Shuyong, Li, Qinxi, Rui, Hongliang, Luo, Wen, ...

Axin and p53 are tumor suppressors, controlling cell growth, apoptosis, and development. We show that Axin interacts with homeodomain-interacting protein kinase-2 (HIPK2), which is linked to...

Two-Dimensional Infrared Correlation Spectroscopy Study of Sequential Events in the Heat-Induced Unfolding and Aggregation Process of Myoglobin

Yan, Yong-Bin, Wang, Qi, He, Hua-Wei, Hu, Xin-Yao, Zhang, Ri-Qing, Zhou, Hai-Meng

Unfolding and aggregation are basic problems in protein science with serious biotechnological and medical implications. Probing the sequential events occurring during the unfolding and aggregation...

Protein Thermal Aggregation Involves Distinct Regions: Sequential Events in the Heat-Induced Unfolding and Aggregation of Hemoglobin

Yan, Yong-Bin, Wang, Qi, He, Hua-Wei, Zhou, Hai-Meng

Protein thermal aggregation plays a crucial role in protein science and engineering. Despite its biological importance, little is known about the mechanism and pathway(s) involved in the formation of...

Osmophobic Effect of Glycerol on Irreversible Thermal Denaturation of Rabbit Creatine Kinase

Meng, Fan-Guo, Hong, Yuan-Kai, He, Hua-Wei, Lyubarev, Arkadii E., Kurganov, Boris I., Yan, Yong-Bin, ...

Protein stability plays an extremely important role not only in its biological function but also in medical science and protein engineering. Osmolytes provide a general method to protect proteins...

Conformational Change in the C-Terminal Domain Is Responsible for the Initiation of Creatine Kinase Thermal Aggregation

He, Hua-Wei, Zhang, Jun, Zhou, Hai-Meng, Yan, Yong-Bin

Protein conformational changes may be associated with particular properties such as its function, transportation, assembly, tendency to aggregate, and potential cytotoxicity. In this research, the...

Axin stimulates p53 functions by activation of HIPK2 kinase through multimeric complex formation

Rui, Yanning, Xu, Zhen, Lin, Shuyong, Li, Qinxi, Rui, Hongliang, Luo, Wen, ...

Axin and p53 are tumor suppressors, controlling cell growth, apoptosis, and development. We show that Axin interacts with homeodomain-interacting protein kinase-2 (HIPK2), which is linked to...

Two-Dimensional Infrared Correlation Spectroscopy Study of Sequential Events in the Heat-Induced Unfolding and Aggregation Process of Myoglobin

Yan, Yong-Bin, Wang, Qi, He, Hua-Wei, Hu, Xin-Yao, Zhang, Ri-Qing, Zhou, Hai-Meng

Unfolding and aggregation are basic problems in protein science with serious biotechnological and medical implications. Probing the sequential events occurring during the unfolding and aggregation...

Protein Thermal Aggregation Involves Distinct Regions: Sequential Events in the Heat-Induced Unfolding and Aggregation of Hemoglobin

Yan, Yong-Bin, Wang, Qi, He, Hua-Wei, Zhou, Hai-Meng

Protein thermal aggregation plays a crucial role in protein science and engineering. Despite its biological importance, little is known about the mechanism and pathway(s) involved in the formation of...

Osmophobic Effect of Glycerol on Irreversible Thermal Denaturation of Rabbit Creatine Kinase

Meng, Fan-Guo, Hong, Yuan-Kai, He, Hua-Wei, Lyubarev, Arkadii E., Kurganov, Boris I., Yan, Yong-Bin, ...

Protein stability plays an extremely important role not only in its biological function but also in medical science and protein engineering. Osmolytes provide a general method to protect proteins...

Conformational Change in the C-Terminal Domain Is Responsible for the Initiation of Creatine Kinase Thermal Aggregation

He, Hua-Wei, Zhang, Jun, Zhou, Hai-Meng, Yan, Yong-Bin

Protein conformational changes may be associated with particular properties such as its function, transportation, assembly, tendency to aggregate, and potential cytotoxicity. In this research, the...

Oligomerization and Aggregation of Bovine Pancreatic Ribonuclease A: Characteristic Events Observed by FTIR Spectroscopy

Yan, Yong-Bin, Zhang, Jun, He, Hua-Wei, Zhou, Hai-Meng

Nonnative protein aggregation, which is a common feature in biotechnology, is also a clinical feature in more than 20 serious degenerative diseases. We studied the specific events of bovine...

Assisting the Reactivation of Guanidine Hydrochloride-Denatured Aminoacylase by Hydroxypropyl Cyclodextrins

Kim, Sung-Hye, Zhang, Jun, Jiang, Yan, Zhou, Hai-Meng, Yan, Yong-Bin

Cyclodextrin is a water-soluble circular oligosaccharide with a cylinder shape characterized by exterior hydrophilic rims and an interior hydrophobic cavity, which makes it an ideal additive to...

The AKAP Yu is required for olfactory long-term memory formation in Drosophila

Lu, Yubing, Lu, Yi-Sheng, Shuai, Yichun, Feng, Chunhua, Tully, Tim, Xie, Zuoping, ...

Extensive neurogenetic analysis has shown that memory formation depends critically on cAMP-protein kinase A (PKA) signaling. Details of how this pathway is involved in memory formation, however,...

Capture of monomeric refolding intermediate of human muscle creatine kinase

Li, Sen, Bai, Ji-Hong, Park, Yong-Doo, Zhou, Hai-Meng

Human muscle creatine kinase (CK) is an enzyme that plays an important physiological role in the energy metabolism of humans. It also serves as a typical model for studying refolding of proteins. A...

Unassisted refolding of urea-denatured arginine kinase from shrimp Feneropenaeus chinensis: Evidence for two equilibrium intermediates in the refolding pathway

Pan, Ji-Cheng, Yu, Zhenhang, Su, Xiao-Yang, Sun, Ye-Qing, Rao, Xue-Ming, Zhou, Hai-Meng

The refolding process and the equilibrium intermediates of urea-denatured arginine kinase (AK) were investigated by 1-anilino-8-naphthalenesulfonate (ANS) intrinsic fluorescence, far-UV circular...

Two-phase unfolding pathway of ribonuclease A during denaturation induced by dithiothreitol

Yan, Yong-Bin, Jiang, Bo, Zhang, Ri-Qing, Zhou, Hai-Meng

The dynamics of the unfolding process of bovine pancreatic ribonuclease A (RNase A) unfolded by dithiothreitol (DTT) at a low concentration of 1:30 were investigated in alkaline phosphate-buffered...

Chaperone-like activity of peptidyl-prolyl cis-trans isomerase during creatine kinase refolding

Ou, Wen-Bin, Luo, Wei, Park, Yong-Doo, Zhou, Hai-Meng

Porcine kidney 18 kD peptidyl-prolyl cis-trans isomerase (PPIase) belongs to the cyclophilin family that is inhibited by the immunosuppressive drug cyclosporin A. The chaperone activity of PPIase was...