Guillaume Chanfreau

Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III

Chanfreau, Guillaume, Buckle, Malcolm, Jacquier, Alain

Ribonucleases III are double-stranded RNA (dsRNA) endonucleases required for the processing of a large number of prokaryotic and eukaryotic transcripts. Although the specificity of bacterial RNase...

Precursors to the U3 Small Nucleolar RNA Lack Small Nucleolar RNP Proteins but Are Stabilized by La Binding

Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David

Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...

A novel family of RNA tetraloop structure forms the recognition site for Saccharomyces cerevisiae RNase III

Wu, Haihong, Yang, Pok K., Butcher, Samuel E., Kang, Sundeep, Chanfreau, Guillaume, Feigon, Juli

RNases III are a family of double-stranded RNA (dsRNA) endoribonucleases involved in the processing and decay of a large number of cellular RNAs as well as in RNA interference. The dsRNA substrates...

Conservation of RNase III Processing Pathways and Specificity in Hemiascomycetes

Chanfreau, Guillaume

Rnt1p, the only known Saccharomyces cerevisiae RNase III endonuclease, plays important functions in the processing of precursors of rRNAs (pre-rRNAs) and of a large number of small nuclear RNAs...

Alternative 3′-end processing of U5 snRNA by RNase III

Chanfreau, Guillaume, Elela, Sherif Abou, Ares, Manuel, Guthrie, Christine

The cellular components required to form the 3′ ends of small nuclear RNAs are unknown. U5 snRNA from Saccharomyces cerevisiae is found in two forms that differ in length at their 3′ ends (U5L...

Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase III

Wu, Haihong, Henras, Anthony, Chanfreau, Guillaume, Feigon, Juli

Specific recognition of double-stranded RNA (dsRNA) by dsRNA-binding domains (dsRBDs) is involved in a large number of biological and regulatory processes. Although structures of dsRBDs in complex...

Cotranscriptional Recruitment of the Pseudouridylsynthetase Cbf5p and of the RNA Binding Protein Naf1p during H/ACA snoRNP Assembly

Yang, Pok Kwan, Hoareau, Coralie, Froment, Carine, Monsarrat, Bernard, Henry, Yves, Chanfreau, Guillaume

H/ACA small nucleolar ribonucleoprotein particles (snoRNPs) are essential for the maturation and pseudouridylation of the precursor of rRNAs and other stable RNAs. Although the RNA and protein...

The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5′-end processing of S. cerevisiae box C/D snoRNAs

LEE, CHRISSIE YOUNG, LEE, ALBERT, CHANFREAU, GUILLAUME

Small nucleolar RNAs (snoRNAs) play important roles in ribosomal RNA metabolism. In Saccharomyces cerevisiae, box C/D snoRNAs are synthesized from excised introns, polycistronic precursors, or...

A cotranscriptional model for 3′-end processing of the Saccharomyces cerevisiae pre-ribosomal RNA precursor

HENRAS, ANTHONY K., BERTRAND, EDOUARD, CHANFREAU, GUILLAUME

Cleavage of the Saccharomyces cerevisiae primary ribosomal RNA (rRNA) transcript in the 3′ external transcribed spacer (ETS) by Rnt1p generates the 35S pre-rRNA, the earliest detectable species in...

Recognition of a conserved class of RNA tetraloops by Saccharomyces cerevisiae RNase III

Chanfreau, Guillaume, Buckle, Malcolm, Jacquier, Alain

Ribonucleases III are double-stranded RNA (dsRNA) endonucleases required for the processing of a large number of prokaryotic and eukaryotic transcripts. Although the specificity of bacterial RNase...

Precursors to the U3 Small Nucleolar RNA Lack Small Nucleolar RNP Proteins but Are Stabilized by La Binding

Kufel, Joanna, Allmang, Christine, Chanfreau, Guillaume, Petfalski, Elisabeth, Lafontaine, Denis L. J., Tollervey, David

Almost all small eukaryotic RNAs are processed from transiently stabilized 3′-extended forms. A key question is how and why such intermediates are stabilized and how they can then be processed to...

A novel family of RNA tetraloop structure forms the recognition site for Saccharomyces cerevisiae RNase III

Wu, Haihong, Yang, Pok K., Butcher, Samuel E., Kang, Sundeep, Chanfreau, Guillaume, Feigon, Juli

RNases III are a family of double-stranded RNA (dsRNA) endoribonucleases involved in the processing and decay of a large number of cellular RNAs as well as in RNA interference. The dsRNA substrates...

Conservation of RNase III Processing Pathways and Specificity in Hemiascomycetes

Chanfreau, Guillaume

Rnt1p, the only known Saccharomyces cerevisiae RNase III endonuclease, plays important functions in the processing of precursors of rRNAs (pre-rRNAs) and of a large number of small nuclear RNAs...

Alternative 3′-end processing of U5 snRNA by RNase III

Chanfreau, Guillaume, Elela, Sherif Abou, Ares, Manuel, Guthrie, Christine

The cellular components required to form the 3′ ends of small nuclear RNAs are unknown. U5 snRNA from Saccharomyces cerevisiae is found in two forms that differ in length at their 3′ ends (U5L...

Structural basis for recognition of the AGNN tetraloop RNA fold by the double-stranded RNA-binding domain of Rnt1p RNase III

Wu, Haihong, Henras, Anthony, Chanfreau, Guillaume, Feigon, Juli

Specific recognition of double-stranded RNA (dsRNA) by dsRNA-binding domains (dsRBDs) is involved in a large number of biological and regulatory processes. Although structures of dsRBDs in complex...

Cotranscriptional Recruitment of the Pseudouridylsynthetase Cbf5p and of the RNA Binding Protein Naf1p during H/ACA snoRNP Assembly

Yang, Pok Kwan, Hoareau, Coralie, Froment, Carine, Monsarrat, Bernard, Henry, Yves, Chanfreau, Guillaume

H/ACA small nucleolar ribonucleoprotein particles (snoRNPs) are essential for the maturation and pseudouridylation of the precursor of rRNAs and other stable RNAs. Although the RNA and protein...

The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5′-end processing of S. cerevisiae box C/D snoRNAs

LEE, CHRISSIE YOUNG, LEE, ALBERT, CHANFREAU, GUILLAUME

Small nucleolar RNAs (snoRNAs) play important roles in ribosomal RNA metabolism. In Saccharomyces cerevisiae, box C/D snoRNAs are synthesized from excised introns, polycistronic precursors, or...

A cotranscriptional model for 3′-end processing of the Saccharomyces cerevisiae pre-ribosomal RNA precursor

HENRAS, ANTHONY K., BERTRAND, EDOUARD, CHANFREAU, GUILLAUME

Cleavage of the Saccharomyces cerevisiae primary ribosomal RNA (rRNA) transcript in the 3′ external transcribed spacer (ETS) by Rnt1p generates the 35S pre-rRNA, the earliest detectable species in...