Xu, Xue-Ming, Carlson, Bradley A., Zhang, Yan, Mix, Heiko, Kryukov, Gregory V., Glass, Richard S., ...
We used comparative genomics and experimental analyses to show that (1) eukaryotes and archaea, which possess the selenocysteine (Sec) protein insertion machinery contain an enzyme,...
Selenoprotein H Is a Nucleolar Thioredoxin-like Protein with a Unique Expression Pattern (2007)
Novoselov, Sergey V., Kryukov, Gregory V., Xu, Xue-Ming, Carlson, Bradley A., Hatfield, Dolph L., Gladyshev, Vadim N.
The human selenoproteome consists of 25 known selenoproteins, but functions of many of these proteins are not known. Selenoprotein H (SelH) is a recently discovered 14-kDa mammalian protein with no...
Kryukov, Gregory V., Pennacchio, Len A., Sunyaev, Shamil R.
The accumulation of mildly deleterious missense mutations in individual human genomes has been proposed to be a genetic basis for complex diseases. The plausibility of this hypothesis depends on...
Is there a twenty third amino acid in the genetic code? (2006)
Lobanov, Alexey V., Kryukov, Gregory V., Hatfield, Dolph L., Gladyshev, Vadim
The universal genetic code includes 20 common amino acids. In addition, selenocysteine (Sec) and pyrrolysine (Pyl), known as the twenty first and twenty second amino acids, are encoded by UGA and...
The Plasmodium selenoproteome (2006)
Lobanov, Alexey V., Delgado, Cesar, Rahlfs, Stefan, Novoselov, Sergey V., Kryukov, Gregory V., Gromer, Stephan, ...
The use of selenocysteine (Sec) as the 21st amino acid in the genetic code has been described in all three major domains of life. However, within eukaryotes, selenoproteins are only known in animals...
Taskov, Kalin, Chapple, Charles, Kryukov, Gregory V., Castenello, Sergi, Lobanov, Alexei V., Korotkov, Konstantin V., ...
Selenocysteine (Sec) is co-translationally inserted into selenoproteins in response to codon UGA with the help of the selenocysteine insertion sequence (SECIS) element.The number of...
Identification and characterization of phosphoseryl-tRNA [Ser]Sec kinase (2004)
Carlson, Bradley A., Xu, Xue-Ming, Kryukov, Gregory V., Rao, Mahadev, Berry, Marla J., Gladyshev, Vadim N., ...
In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA...
The prokaryotic selenoproteome (2004)
Kryukov, Gregory V., Gladyshev, Vadim N.
In the genetic code, the UGA codon has a dual function as it encodes selenocysteine (Sec) and serves as a stop signal. However, only the translation terminator function is used in gene annotation...
Castellano, Sergi, Novoselov, Sergey V., Kryukov, Gregory V., Lescure, Alain, Blanco, Enrique, Krol, Alain, ...
While the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. The dual role of the UGA codon confounds the...
Castellano, Sergi, Novoselov, Sergey V., Kryukov, Gregory V., Lescure, Alain, Blanco, Enrique, Krol, Alain, ...
While the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. The dual role of the UGA codon confounds the...
Characterization of Mammalian Selenoproteomes (2003)
Kryukov, Gregory V., Castellano, Sergi, Novoselov, Sergey V., Lobanov, Alexey V., Zehtab, Omid, Guigo, Roderic, ...
In the genetic code, UGA serves as a stop signal and a selenocysteine codon, but no computational methods for identifying its coding function are available. Consequently, most selenoprotein genes are...
Novoselov, Sergey V., Rao, Mahadev, Onoshko, Natalia V., Zhi, Huijun, Kryukov, Gregory V., Xiang, Youbin, ...
Known eukaryotic selenocysteine (Sec)-containing proteins are animal proteins, whereas selenoproteins have not been found in yeast and plants. Surprisingly, we detected selenoproteins in a member of...
Selenium Metabolism in Drosophila (2001)
Martin-Romero, F. Javier, Kryukov, Gregory V., Lobanov, Alexei V., Carlson, Bradley A., Lee, Byeong Jae, Gladyshev, Vadim N., ...
Selenocysteine is a rare amino acid in protein that is encoded by UGA with the requirement of a downstream mRNA stem-loop structure, the selenocysteine insertion sequence element. To detect...
Identification and Characterization of a New Mammalian Glutaredoxin (Thioltransferase), Grx2 (2001)
Gladyshev, Vadim N., Liu, Aimin, Novoselov, Sergey V., Krysan, Konstantin, Sun, Qi-An, Kryukov, Valentin M., ...
A thiol/disulfide oxidoreductase component of the GSH system, glutaredoxin (Grx), is involved in the reduction of GSH-based mixed disulfides and participates in a variety of cellular redox pathways....
Moustafa, Mohamed E., Carlson, Bradley A., El-Saadani, Muhammed A., Kryukov, Gregory V., Sun, Qi-An, Harney, John W., ...
Selenocysteine (Sec) tRNA (tRNA [Ser]Sec) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein...
Kryukov, Gregory V., Kryukov, Valentin M., Gladyshev, Vadim N.
Mammalian selenium-containing proteins identified thus far contain selenium in the form of a selenocysteine residue encoded by UGA. These proteins lack common amino acid sequence motifs, but...
Moustafa, Mohamed E., Carlson, Bradley A., El-Saadani, Muhammad A., Kryukov, Gregory V., Sun, Qi-An, Harney, John W., ...
Selenocysteine (Sec) tRNA (tRNA[Ser]Sec) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein...
Selenoprotein R is a zinc-containing stereo-specific methionine sulfoxide reductase
Kryukov, Gregory V., Kumar, R. Abhilash, Koc, Ahmet, Sun, Zhaohui, Gladyshev, Vadim N.
Selenoprotein R (SelR) is a mammalian selenocysteine-containing protein with no known function. Here we report that cysteine homologs of SelR are present in all organisms except certain parasites and...
Novoselov, Sergey V., Rao, Mahadev, Onoshko, Natalia V., Zhi, Huijun, Kryukov, Gregory V., Xiang, Youbin, ...
Known eukaryotic selenocysteine (Sec)-containing proteins are animal proteins, whereas selenoproteins have not been found in yeast and plants. Surprisingly, we detected selenoproteins in a member of...
Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase
Carlson, Bradley A., Xu, Xue-Ming, Kryukov, Gregory V., Rao, Mahadev, Berry, Marla J., Gladyshev, Vadim N., ...
In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA...
Taskov, Kalin, Chapple, Charles, Kryukov, Gregory V., Castellano, Sergi, Lobanov, Alexey V., Korotkov, Konstantin V., ...
Selenocysteine (Sec) is co-translationally inserted into selenoproteins in response to codon UGA with the help of the selenocysteine insertion sequence (SECIS) element. The number of selenoproteins...
Castellano, Sergi, Novoselov, Sergey V, Kryukov, Gregory V, Lescure, Alain, Blanco, Enrique, Krol, Alain, ...
While the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. The dual role of the UGA codon confounds the...
The prokaryotic selenoproteome
Kryukov, Gregory V, Gladyshev, Vadim N
In the genetic code, the UGA codon has a dual function as it encodes selenocysteine (Sec) and serves as a stop signal. However, only the translation terminator function is used in gene annotation...
Lobanov, Alexey V., Delgado, Cesar, Rahlfs, Stefan, Novoselov, Sergey V., Kryukov, Gregory V., Gromer, Stephan, ...
The use of selenocysteine (Sec) as the 21st amino acid in the genetic code has been described in all three major domains of life. However, within eukaryotes, selenoproteins are only known in animals...
Evolutionary constraints in conserved nongenic sequences of mammals
Keightley, Peter D., Kryukov, Gregory V., Sunyaev, Shamil, Halligan, Daniel L., Gaffney, Daniel J.
Mammalian genomes contain many highly conserved nongenic sequences (CNGs) whose functional significance is poorly understood. Sets of CNGs have previously been identified by selecting the most...
Moustafa, Mohamed E., Carlson, Bradley A., El-Saadani, Muhammad A., Kryukov, Gregory V., Sun, Qi-An, Harney, John W., ...
Selenocysteine (Sec) tRNA (tRNA[Ser]Sec) serves as both the site of Sec biosynthesis and the adapter molecule for donation of this amino acid to protein. The consequences on selenoprotein...
Selenoprotein R is a zinc-containing stereo-specific methionine sulfoxide reductase
Kryukov, Gregory V., Kumar, R. Abhilash, Koc, Ahmet, Sun, Zhaohui, Gladyshev, Vadim N.
Selenoprotein R (SelR) is a mammalian selenocysteine-containing protein with no known function. Here we report that cysteine homologs of SelR are present in all organisms except certain parasites and...
Novoselov, Sergey V., Rao, Mahadev, Onoshko, Natalia V., Zhi, Huijun, Kryukov, Gregory V., Xiang, Youbin, ...
Known eukaryotic selenocysteine (Sec)-containing proteins are animal proteins, whereas selenoproteins have not been found in yeast and plants. Surprisingly, we detected selenoproteins in a member of...
Identification and characterization of phosphoseryl-tRNA[Ser]Sec kinase
Carlson, Bradley A., Xu, Xue-Ming, Kryukov, Gregory V., Rao, Mahadev, Berry, Marla J., Gladyshev, Vadim N., ...
In 1970, a kinase activity that phosphorylated a minor species of seryl-tRNA to form phosphoseryl-tRNA was found in rooster liver [Maenpaa, P. H. & Bernfield, M. R. (1970) Proc. Natl. Acad. Sci. USA...
Taskov, Kalin, Chapple, Charles, Kryukov, Gregory V., Castellano, Sergi, Lobanov, Alexey V., Korotkov, Konstantin V., ...
Selenocysteine (Sec) is co-translationally inserted into selenoproteins in response to codon UGA with the help of the selenocysteine insertion sequence (SECIS) element. The number of selenoproteins...
Evolutionary constraints in conserved nongenic sequences of mammals
Keightley, Peter D., Kryukov, Gregory V., Sunyaev, Shamil, Halligan, Daniel L., Gaffney, Daniel J.
Mammalian genomes contain many highly conserved nongenic sequences (CNGs) whose functional significance is poorly understood. Sets of CNGs have previously been identified by selecting the most...
Castellano, Sergi, Novoselov, Sergey V, Kryukov, Gregory V, Lescure, Alain, Blanco, Enrique, Krol, Alain, ...
While the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. The dual role of the UGA codon confounds the...
The prokaryotic selenoproteome
Kryukov, Gregory V, Gladyshev, Vadim N
In the genetic code, the UGA codon has a dual function as it encodes selenocysteine (Sec) and serves as a stop signal. However, only the translation terminator function is used in gene annotation...
Lobanov, Alexey V., Delgado, Cesar, Rahlfs, Stefan, Novoselov, Sergey V., Kryukov, Gregory V., Gromer, Stephan, ...
The use of selenocysteine (Sec) as the 21st amino acid in the genetic code has been described in all three major domains of life. However, within eukaryotes, selenoproteins are only known in animals...
Kryukov, Gregory V., Pennacchio, Len A., Sunyaev, Shamil R.
The accumulation of mildly deleterious missense mutations in individual human genomes has been proposed to be a genetic basis for complex diseases. The plausibility of this hypothesis depends on...