Gohta Goshima

Length control of the metaphase spindle (2005)

Goshima, Gohta, Wollman, Roy, Stuurman, Nico, Scholey, Jonathan M., Vale, Ronald D.

Author Posting. © Elsevier B. V., 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version...

Length control of the metaphase spindle (2005)

Goshima, Gohta, Wollman, Roy, Stuurman, Nico, Scholey, Jonathan M., Vale, Ronald D.

Author Posting. © Elsevier B. V., 2005. This is the author's version of the work. It is posted here by permission of Elsevier B. V. for personal use, not for redistribution. The definitive version...

The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore

Goshima, Gohta, Iwasaki, Osamu, Obuse, Chikashi, Yanagida, Mitsuhiro

Mis12 is a kinetochore protein essential for equal chromosome segregation and is evolutionarily conserved from yeast to human. In this study, we report the isolation and characterization of...

Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation

Goshima, Gohta, Saitoh, Shigeaki, Yanagida, Mitsuhiro

High-fidelity chromosome transmission is fundamental in controlling the quality of the cell division cycle. The spindle pole-to-pole distance remains constant from metaphase to anaphase A. We show...

Cell Cycle-dependent Dynamics and Regulation of Mitotic Kinesins in Drosophila S2 CellsD⃞

Goshima, Gohta, Vale, Ronald D.

Constructing a mitotic spindle requires the coordinated actions of several kinesin motor proteins. Here, we have visualized the dynamics of five green fluorescent protein (GFP)-tagged mitotic...

The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore

Goshima, Gohta, Iwasaki, Osamu, Obuse, Chikashi, Yanagida, Mitsuhiro

Mis12 is a kinetochore protein essential for equal chromosome segregation and is evolutionarily conserved from yeast to human. In this study, we report the isolation and characterization of...

Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation

Goshima, Gohta, Saitoh, Shigeaki, Yanagida, Mitsuhiro

High-fidelity chromosome transmission is fundamental in controlling the quality of the cell division cycle. The spindle pole-to-pole distance remains constant from metaphase to anaphase A. We show...

Cell Cycle-dependent Dynamics and Regulation of Mitotic Kinesins in Drosophila S2 CellsD⃞

Goshima, Gohta, Vale, Ronald D.

Constructing a mitotic spindle requires the coordinated actions of several kinesin motor proteins. Here, we have visualized the dynamics of five green fluorescent protein (GFP)-tagged mitotic...

Mechanisms for focusing mitotic spindle poles by minus end–directed motor proteins

Goshima, Gohta, Nédélec, François, Vale, Ronald D.

During the formation of the metaphase spindle in animal somatic cells, kinetochore microtubule bundles (K fibers) are often disconnected from centrosomes, because they are released from centrosomes...

Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway

Goshima, Gohta, Kiyomitsu, Tomomi, Yoda, Kinya, Yanagida, Mitsuhiro

Kinetochores are the chromosomal sites for spindle interaction and play a vital role for chromosome segregation. The composition of kinetochore proteins and their cellular roles are, however, poorly...

The roles of microtubule-based motor proteins in mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line

Goshima, Gohta, Vale, Ronald D.

Kinesins and dyneins play important roles during cell division. Using RNA interference (RNAi) to deplete individual (or combinations of) motors followed by immunofluorescence and time-lapse...

Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle

Goshima, Gohta, Mayer, Mirjam, Zhang, Nan, Stuurman, Nico, Vale, Ronald D.

Since the discovery of γ-tubulin, attention has focused on its involvement as a microtubule nucleator at the centrosome. However, mislocalization of γ-tubulin away from the centrosome does not...