Gary Ruvkun

Lifespan Regulation by Evolutionarily Conserved Genes Essential for Viability (2007)

Sean P. Curran, Gary Ruvkun

Evolutionarily conserved mechanisms that control aging are predicted to have prereproductive functions in order to be subject to natural selection. Genes that are essential for growth and development...

Lifespan regulation by evolutionarily conserved genes essential for viability (2007)

Sean Patrick Curran, Gary Ruvkun

Evolutionarily conserved mechanisms that control aging are predicted to have pre-reproductive functions in order to be subject to natural selection. Genes that are essential for growth and...

Two Membrane-Associated Tyrosine Phosphatase Homologs Potentiate C. elegans AKT-1/PKB Signaling (2006)

Patrick J. Hu, Jinling Xu, Gary Ruvkun

Akt/protein kinase B (PKB) functions in conserved signaling cascades that regulate growth and metabolism. In humans, Akt/PKB is dysregulated in diabetes and cancer; in Caenorhabditis elegans, Akt/PKB...

Functional proteomics reveals the biochemical niche of C. elegans DCR-1 in multiple small-RNA-mediated pathways (2006)

Duchaine, Thomas F., Wohlschlegel, James A., Kennedy, Scott, Bei, Yanxia, Conte, Darryl, Pang, Ka Ming, ...

In plants, animals, and fungi, members of the Dicer family of RNase III-related enzymes process double-stranded RNA (dsRNA) to initiate small-RNA-mediated gene-silencing mechanisms. To learn how C....

Functional Genomic Analysis of C. elegans Molting (2005)

Alison R. Frand, Sascha Russel, Gary Ruvkun

The authors use a genome-wide RNA-interference screen to identify and characterize genes involved in C. elegans molting. They investigate regulatory networks involved in molting, lending important...

Functional Genomic Analysis of C. elegans Molting (2005)

Alison R. Frand, Sascha Russel, Gary Ruvkun

Although the molting cycle is a hallmark of insects and nematodes, neither the endocrine control of molting via size, stage, and nutritional inputs nor the enzymatic mechanism for synthesis and...

Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing (2001)

Grishok, Alla, Pasquinelli, Amy E., Conte, Darryl, Li, Na, Parrish, Susan, Ha, Ilho, ...

RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and...

A common muscarinic pathway for diapause recovery in the distantly related nematode species Caenorhabditis elegans and Ancylostoma caninum

Tissenbaum, Heidi A., Hawdon, John, Perregaux, Melissa, Hotez, Peter, Guarente, Leonard, Ruvkun, Gary

Converging TGF-β and insulin-like neuroendocrine signaling pathways regulate whether Caenorhabditis elegans develops reproductively or arrests at the dauer larval stage. We examined whether...

DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in HepG2 cells

Nasrin, Nargis, Ogg, Scott, Cahill, Catherine M., Biggs, William, Nui, Simin, Dore, Justin, ...

Insulin negatively regulates expression of the insulin-like growth factor binding protein 1 (IGFBP-1) gene by means of an insulin-responsive element (IRE) that also contributes to glucocorticoid...

Activity of the Caenorhabditis elegans UNC-86 POU transcription factor modulates olfactory sensitivity

Sze, Ji Ying, Ruvkun, Gary

The activity of transcription factors modulates several neural pathways that mediate complex behaviors. We describe here the role of the POU transcription factor UNC-86 in the olfactory behavior of...

daf-28 encodes a C. elegans insulin superfamily member that is regulated by environmental cues and acts in the DAF-2 signaling pathway

Li, Weiqing, Kennedy, Scott G., Ruvkun, Gary

In Caenorhabditis elegans, the decision to enter a developmentally arrested dauer larval stage is triggered by a combination of signals from sensory neurons in response to environmental cues, which...

Identification of many microRNAs that copurify with polyribosomes in mammalian neurons

Kim, John, Krichevsky, Anna, Grad, Yonatan, Hayes, Gabriel D., Kosik, Kenneth S., Church, George M., ...

Localized translation in mammalian dendrites may play a role in synaptic plasticity and contribute to the molecular basis for learning and memory. The regulatory mechanisms that control localized...

The DAF-3 Smad protein antagonizes TGF-β-related receptor signaling in the Caenorhabditis elegans dauer pathway

Patterson, Garth I., Koweek, Allison, Wong, Arthur, Liu, Yanxia, Ruvkun, Gary

Signals from TGF-β superfamily receptors are transduced to the nucleus by Smad proteins, which transcriptionally activate target genes. In Caenorhabditis elegans, defects in a TGF-β-related pathway...

A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans

Paradis, Suzanne, Ailion, Michael, Toker, Alex, Thomas, James H., Ruvkun, Gary

An insulin receptor-like signaling pathway regulates Caenorhabditis elegans metabolism, development, and longevity. Inactivation of the insulin receptor homolog DAF-2, the AGE-1 PI3K, or the AKT-1...

Alternative olfactory neuron fates are specified by the LIM homeobox gene lim-4

Sagasti, Alvaro, Hobert, Oliver, Troemel, Emily R., Ruvkun, Gary, Bargmann, Cornelia I.

The Caenorhabditis elegans AWA, AWB, and AWC olfactory neurons are each required for the recognition of a specific subset of volatile odorants. lim-4 mutants express an AWC reporter gene...

Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor

Paradis, Suzanne, Ruvkun, Gary

A neurosecretory pathway regulates a reversible developmental arrest and metabolic shift at the Caenorhabditis elegans dauer larval stage. Defects in an insulin-like signaling pathway cause arrest at...

A systematic RNAi screen for longevity genes in C. elegans

Hamilton, Benjamin, Dong, Yuqing, Shindo, Mami, Liu, Wenyu, Odell, Ian, Ruvkun, Gary, ...

We report here the first genome-wide functional genomic screen for longevity genes. We systematically surveyed Caenorhabditis elegans genes using large-scale RNA interference (RNAi), and found that...

Functional Genomic Analysis of C. elegans Molting

Frand, Alison R, Russel, Sascha, Ruvkun, Gary

Although the molting cycle is a hallmark of insects and nematodes, neither the endocrine control of molting via size, stage, and nutritional inputs nor the enzymatic mechanism for synthesis and...

A uniform system for microRNA annotation

AMBROS, VICTOR, BARTEL, BONNIE, BARTEL, DAVID P., BURGE, CHRISTOPHER B., CARRINGTON, JAMES C., CHEN, XUEMEI, ...

MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characteristic hairpin secondary structure. Guidelines are presented for...

Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the unusually large and diverse C. elegans insulin gene family

Pierce, Sarah B., Costa, Michael, Wisotzkey, Robert, Devadhar, Sharmila, Homburger, Sheila A., Buchman, Andrew R., ...

The activity of the DAF-2 insulin-like receptor is required for Caenorhabditis elegans reproductive growth and normal adult life span. Informatic analysis identified 37 C. elegans genes predicted to...

Two Membrane-Associated Tyrosine Phosphatase Homologs Potentiate C. elegans AKT-1/PKB Signaling

Hu, Patrick J, Xu, Jinling, Ruvkun, Gary

Akt/protein kinase B (PKB) functions in conserved signaling cascades that regulate growth and metabolism. In humans, Akt/PKB is dysregulated in diabetes and cancer; in Caenorhabditis elegans, Akt/PKB...

A common muscarinic pathway for diapause recovery in the distantly related nematode species Caenorhabditis elegans and Ancylostoma caninum

Tissenbaum, Heidi A., Hawdon, John, Perregaux, Melissa, Hotez, Peter, Guarente, Leonard, Ruvkun, Gary

Converging TGF-β and insulin-like neuroendocrine signaling pathways regulate whether Caenorhabditis elegans develops reproductively or arrests at the dauer larval stage. We examined whether...

DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in HepG2 cells

Nasrin, Nargis, Ogg, Scott, Cahill, Catherine M., Biggs, William, Nui, Simin, Dore, Justin, ...

Insulin negatively regulates expression of the insulin-like growth factor binding protein 1 (IGFBP-1) gene by means of an insulin-responsive element (IRE) that also contributes to glucocorticoid...

Activity of the Caenorhabditis elegans UNC-86 POU transcription factor modulates olfactory sensitivity

Sze, Ji Ying, Ruvkun, Gary

The activity of transcription factors modulates several neural pathways that mediate complex behaviors. We describe here the role of the POU transcription factor UNC-86 in the olfactory behavior of...

daf-28 encodes a C. elegans insulin superfamily member that is regulated by environmental cues and acts in the DAF-2 signaling pathway

Li, Weiqing, Kennedy, Scott G., Ruvkun, Gary

In Caenorhabditis elegans, the decision to enter a developmentally arrested dauer larval stage is triggered by a combination of signals from sensory neurons in response to environmental cues, which...

Regulation of DAF-2 receptor signaling by human insulin and ins-1, a member of the unusually large and diverse C. elegans insulin gene family

Pierce, Sarah B., Costa, Michael, Wisotzkey, Robert, Devadhar, Sharmila, Homburger, Sheila A., Buchman, Andrew R., ...

The activity of the DAF-2 insulin-like receptor is required for Caenorhabditis elegans reproductive growth and normal adult life span. Informatic analysis identified 37 C. elegans genes predicted to...

Identification of many microRNAs that copurify with polyribosomes in mammalian neurons

Kim, John, Krichevsky, Anna, Grad, Yonatan, Hayes, Gabriel D., Kosik, Kenneth S., Church, George M., ...

Localized translation in mammalian dendrites may play a role in synaptic plasticity and contribute to the molecular basis for learning and memory. The regulatory mechanisms that control localized...

The DAF-3 Smad protein antagonizes TGF-β-related receptor signaling in the Caenorhabditis elegans dauer pathway

Patterson, Garth I., Koweek, Allison, Wong, Arthur, Liu, Yanxia, Ruvkun, Gary

Signals from TGF-β superfamily receptors are transduced to the nucleus by Smad proteins, which transcriptionally activate target genes. In Caenorhabditis elegans, defects in a TGF-β-related pathway...

A PDK1 homolog is necessary and sufficient to transduce AGE-1 PI3 kinase signals that regulate diapause in Caenorhabditis elegans

Paradis, Suzanne, Ailion, Michael, Toker, Alex, Thomas, James H., Ruvkun, Gary

An insulin receptor-like signaling pathway regulates Caenorhabditis elegans metabolism, development, and longevity. Inactivation of the insulin receptor homolog DAF-2, the AGE-1 PI3K, or the AKT-1...

Alternative olfactory neuron fates are specified by the LIM homeobox gene lim-4

Sagasti, Alvaro, Hobert, Oliver, Troemel, Emily R., Ruvkun, Gary, Bargmann, Cornelia I.

The Caenorhabditis elegans AWA, AWB, and AWC olfactory neurons are each required for the recognition of a specific subset of volatile odorants. lim-4 mutants express an AWC reporter gene...

Caenorhabditis elegans Akt/PKB transduces insulin receptor-like signals from AGE-1 PI3 kinase to the DAF-16 transcription factor

Paradis, Suzanne, Ruvkun, Gary

A neurosecretory pathway regulates a reversible developmental arrest and metabolic shift at the Caenorhabditis elegans dauer larval stage. Defects in an insulin-like signaling pathway cause arrest at...

A systematic RNAi screen for longevity genes in C. elegans

Hamilton, Benjamin, Dong, Yuqing, Shindo, Mami, Liu, Wenyu, Odell, Ian, Ruvkun, Gary, ...

We report here the first genome-wide functional genomic screen for longevity genes. We systematically surveyed Caenorhabditis elegans genes using large-scale RNA interference (RNAi), and found that...

Functional Genomic Analysis of C. elegans Molting

Frand, Alison R, Russel, Sascha, Ruvkun, Gary

Although the molting cycle is a hallmark of insects and nematodes, neither the endocrine control of molting via size, stage, and nutritional inputs nor the enzymatic mechanism for synthesis and...

A uniform system for microRNA annotation

AMBROS, VICTOR, BARTEL, BONNIE, BARTEL, DAVID P., BURGE, CHRISTOPHER B., CARRINGTON, JAMES C., CHEN, XUEMEI, ...

MicroRNAs (miRNAs) are small noncoding RNA gene products about 22 nt long that are processed by Dicer from precursors with a characteristic hairpin secondary structure. Guidelines are presented for...

Two Membrane-Associated Tyrosine Phosphatase Homologs Potentiate C. elegans AKT-1/PKB Signaling

Hu, Patrick J, Xu, Jinling, Ruvkun, Gary

Akt/protein kinase B (PKB) functions in conserved signaling cascades that regulate growth and metabolism. In humans, Akt/PKB is dysregulated in diabetes and cancer; in Caenorhabditis elegans, Akt/PKB...

Lifespan Regulation by Evolutionarily Conserved Genes Essential for Viability

Curran, Sean P, Ruvkun, Gary

Evolutionarily conserved mechanisms that control aging are predicted to have prereproductive functions in order to be subject to natural selection. Genes that are essential for growth and development...

Gravity Force Transduced by the MEC-4/MEC-10 DEG/ENaC Channel Modulates DAF-16/FoxO Activity in Caenorhabditis elegans

Kim, Nahui, Dempsey, Catherine M., Kuan, Chih-Jen, Zoval, Jim V., O'Rourke, Eyleen, Ruvkun, Gary, ...

The gravity response is an array of behavioral and physiological plasticity elicited by changes in ambient mechanical force and is an evolutionarily ancient adaptive mechanism. We show in...

A Conserved LIM Protein That Affects Muscular Adherens Junction Integrity and Mechanosensory Function in Caenorhabditis elegans

Hobert, Oliver, Moerman, Donald G., Clark, Kathleen A., Beckerle, Mary C., Ruvkun, Gary

We describe here the molecular and functional characterization of the Caenorhabditis elegans unc-97 gene, whose gene product constitutes a novel component of muscular adherens junctions. UNC-97 and...

Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants

Samuelson, Andrew V., Carr, Christopher E., Ruvkun, Gary

Genetic and RNA interference (RNAi) screens for life span regulatory genes have revealed that the daf-2 insulin-like signaling pathway plays a major role in Caenorhabditis elegans longevity. This...

Miniprimer PCR, a New Lens for Viewing the Microbial World▿ †

Isenbarger, Thomas A., Finney, Michael, Ríos-Velázquez, Carlos, Handelsman, Jo, Ruvkun, Gary

Molecular methods based on the 16S rRNA gene sequence are used widely in microbial ecology to reveal the diversity of microbial populations in environmental samples. Here we show that a new PCR...