Frank L. Graham

Site-specific integration mediated by a hybrid adenovirus/adeno-associated virus vector

Recchia, Alessandra, Parks, Robin J., Lamartina, Stefania, Toniatti, Carlo, Pieroni, Luisa, Palombo, Fabio, ...

Adenovirus (Ad) and adeno-associated virus (AAV) have attractive and complementary properties that can be exploited for gene transfer purposes. Ad vectors are probably the most efficient vehicles to...

An adenoviral vector deleted for all viral coding sequences results in enhanced safety and extended expression of a leptin transgene

Morsy, Manal A., Gu, MingCheng, Motzel, Sherri, Zhao, Jing, Lin, Jing, Su, Qin, ...

Adenoviral (Ad)-mediated in vivo gene transfer and expression are limited in part by cellular immune responses to viral-encoded proteins and/or transgene immunogenicity. In an attempt to diminish the...

Administration of helper-dependent adenoviral vectors and sequential delivery of different vector serotype for long-term liver-directed gene transfer in baboons

Morral, Núria, O’Neal, Wanda, Rice, Karen, Leland, Michele, Kaplan, Johanne, Piedra, Pedro A., ...

The efficiency of first-generation adenoviral vectors as gene delivery tools is often limited by the short duration of transgene expression, which can be related to immune responses and to toxic...

Interleukin 12 and B7-1 costimulatory molecule expressed by an adenovirus vector act synergistically to facilitate tumor regression

Pützer, Brigitte M., Hitt, Mary, Muller, William J., Emtage, Peter, Gauldie, Jack, Graham, Frank L.

Stimulation of antitumor immune mechanisms is the primary goal of cancer immunotherapy, and accumulating evidence suggests that effective alteration of the host–tumor relationship involves...

Herpes Simplex Virus gD and Virions Accumulate in Endosomes by Mannose 6-Phosphate-Dependent and -Independent Mechanisms

Brunetti, Craig R., Dingwell, Kevin S., Wale, Cathy, Graham, Frank L., Johnson, David C.

Herpes simplex virus (HSV) glycoprotein D (gD) is modified with mannose 6-phosphate (M6P) and binds to M6P receptors (MPRs). MPRs are involved in the well-characterized pathway by which lysosomal...

Effects of Stuffer DNA on Transgene Expression from Helper-Dependent Adenovirus Vectors

Parks, Robin J., Bramson, Jonathan L., Wan, Yonghong, Addison, Christina L., Graham, Frank L.

We have analyzed transgene (lacZ) expression from a first-generation adenovirus (Ad) vector in comparison to helper-dependent (hd) Ads deleted for various portions of the viral coding sequences and...

Requirement for Both Shc and Phosphatidylinositol 3′ Kinase Signaling Pathways in Polyomavirus Middle T-Mediated Mammary Tumorigenesis

Webster, Marc A., Hutchinson, John N., Rauh, Michael J., Muthuswamy, Senthil K., Anton, Martina, Tortorice, Christopher G., ...

Transgenic mice expressing the polyomavirus (PyV) middle T antigen (MT) develop multifocal mammary tumors which frequently metastasize to the lung. The potent transforming activity of PyV MT is...

ErbB2 Is Required for Muscle Spindle and Myoblast Cell Survival

Andrechek, Eran R., Hardy, William R., Girgis-Gabardo, Adele A., Perry, Robert L. S., Butler, Richard, Graham, Frank L., ...

Signaling mediated by ErbB2 is thought to play a critical role in numerous developmental processes. However, due to the embryonic lethality associated with the germ line inactivation of erbB2, its...

Correction of chromosomal mutation and random integration in embryonic stem cells with helper-dependent adenoviral vectors

Ohbayashi, Fumi, Balamotis, Michael A., Kishimoto, Atsuhiro, Aizawa, Emi, Diaz, Arturo, Hasty, Paul, ...

For gene therapy of inherited diseases, targeted integration/gene repair through homologous recombination (HR) between exogenous and chromosomal DNA would be an ideal strategy to avoid potentially...

Cre Levels Limit Packaging Signal Excision Efficiency in the Cre/loxP Helper-Dependent Adenoviral Vector System

Ng, Philip, Evelegh, Carole, Cummings, Derek, Graham, Frank L.

Helper-dependent (HD) adenovirus vectors devoid of all viral coding sequences have a large cloning capacity and provide long-term transgene expression in vivo with negligible toxicity, making them...

Site-specific integration mediated by a hybrid adenovirus/adeno-associated virus vector

Recchia, Alessandra, Parks, Robin J., Lamartina, Stefania, Toniatti, Carlo, Pieroni, Luisa, Palombo, Fabio, ...

Adenovirus (Ad) and adeno-associated virus (AAV) have attractive and complementary properties that can be exploited for gene transfer purposes. Ad vectors are probably the most efficient vehicles to...

An adenoviral vector deleted for all viral coding sequences results in enhanced safety and extended expression of a leptin transgene

Morsy, Manal A., Gu, MingCheng, Motzel, Sherri, Zhao, Jing, Lin, Jing, Su, Qin, ...

Adenoviral (Ad)-mediated in vivo gene transfer and expression are limited in part by cellular immune responses to viral-encoded proteins and/or transgene immunogenicity. In an attempt to diminish the...

Administration of helper-dependent adenoviral vectors and sequential delivery of different vector serotype for long-term liver-directed gene transfer in baboons

Morral, Núria, O’Neal, Wanda, Rice, Karen, Leland, Michele, Kaplan, Johanne, Piedra, Pedro A., ...

The efficiency of first-generation adenoviral vectors as gene delivery tools is often limited by the short duration of transgene expression, which can be related to immune responses and to toxic...

Interleukin 12 and B7-1 costimulatory molecule expressed by an adenovirus vector act synergistically to facilitate tumor regression

Pützer, Brigitte M., Hitt, Mary, Muller, William J., Emtage, Peter, Gauldie, Jack, Graham, Frank L.

Stimulation of antitumor immune mechanisms is the primary goal of cancer immunotherapy, and accumulating evidence suggests that effective alteration of the host–tumor relationship involves...

Herpes Simplex Virus gD and Virions Accumulate in Endosomes by Mannose 6-Phosphate-Dependent and -Independent Mechanisms

Brunetti, Craig R., Dingwell, Kevin S., Wale, Cathy, Graham, Frank L., Johnson, David C.

Herpes simplex virus (HSV) glycoprotein D (gD) is modified with mannose 6-phosphate (M6P) and binds to M6P receptors (MPRs). MPRs are involved in the well-characterized pathway by which lysosomal...

Effects of Stuffer DNA on Transgene Expression from Helper-Dependent Adenovirus Vectors

Parks, Robin J., Bramson, Jonathan L., Wan, Yonghong, Addison, Christina L., Graham, Frank L.

We have analyzed transgene (lacZ) expression from a first-generation adenovirus (Ad) vector in comparison to helper-dependent (hd) Ads deleted for various portions of the viral coding sequences and...

Requirement for Both Shc and Phosphatidylinositol 3′ Kinase Signaling Pathways in Polyomavirus Middle T-Mediated Mammary Tumorigenesis

Webster, Marc A., Hutchinson, John N., Rauh, Michael J., Muthuswamy, Senthil K., Anton, Martina, Tortorice, Christopher G., ...

Transgenic mice expressing the polyomavirus (PyV) middle T antigen (MT) develop multifocal mammary tumors which frequently metastasize to the lung. The potent transforming activity of PyV MT is...

ErbB2 Is Required for Muscle Spindle and Myoblast Cell Survival

Andrechek, Eran R., Hardy, William R., Girgis-Gabardo, Adele A., Perry, Robert L. S., Butler, Richard, Graham, Frank L., ...

Signaling mediated by ErbB2 is thought to play a critical role in numerous developmental processes. However, due to the embryonic lethality associated with the germ line inactivation of erbB2, its...

Cre Levels Limit Packaging Signal Excision Efficiency in the Cre/loxP Helper-Dependent Adenoviral Vector System

Ng, Philip, Evelegh, Carole, Cummings, Derek, Graham, Frank L.

Helper-dependent (HD) adenovirus vectors devoid of all viral coding sequences have a large cloning capacity and provide long-term transgene expression in vivo with negligible toxicity, making them...

Correction of chromosomal mutation and random integration in embryonic stem cells with helper-dependent adenoviral vectors

Ohbayashi, Fumi, Balamotis, Michael A., Kishimoto, Atsuhiro, Aizawa, Emi, Diaz, Arturo, Hasty, Paul, ...

For gene therapy of inherited diseases, targeted integration/gene repair through homologous recombination (HR) between exogenous and chromosomal DNA would be an ideal strategy to avoid potentially...

Transfer of Tumor Necrosis Factor-α to Rat Lung Induces Severe Pulmonary Inflammation and Patchy Interstitial Fibrogenesis with Induction of Transforming Growth Factor-β1 and Myofibroblasts

Sime, Patricia J., Marr, Robert A., Gauldie, David, Xing, Zhou, Hewlett, Bryan R., Graham, Frank L., ...

Tumor necrosis factor-α is up-regulated in a variety of different human immune-inflammatory and fibrotic pulmonary pathologies. However, its precise role in these pathologies and, in particular, the...